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The map label for this gene is thiN [H]

Identifier: 238916850

GI number: 238916850

Start: 934535

End: 935215

Strand: Direct

Name: thiN [H]

Synonym: EUBELI_00919

Alternate gene names: 238916850

Gene position: 934535-935215 (Clockwise)

Preceding gene: 238916848

Following gene: 238916851

Centisome position: 43.58

GC content: 33.77

Gene sequence:

>681_bases
ATGATATTACAGATTAGTAAGATTAAGGAGATTGATATGAAAACACTTATAGTGACAGGGGGAAGTCTTGATATAAGCTG
GGCAAAGGATTTTGTCAGGACAATAAATGCGGAATACATAATTGCAGCTGACAGTGGCCTTAAATATATTGATAAACTCG
GACTTGTGCCGGATATGATACTGGGGGATTATGATTCAGTAGAAGATGGACTGCTTGATAAGTATAAAAGTATAGATATT
AAGACTTATCCTAAAGAAAAGGATTATACAGATACGCATATTGCTATTATAAATGCGTTGAAAGCCGGAGCGTCAGTCAT
ATATATACTTGGGGCGACAGGTACAAGGATGGATCACACATTTACCAATATATGCAATATGAAGGCTGCACTTGACAGTG
ATGTGCCATGTTTTATATGTGACAGTCACAATAAAATATATCTTATAAATGACAAGATGGGAGAAGTAAAAGTATCAAAA
AAAGGACAGTATGGTGATTATGTATCATTTGTTCCACTATCTGAAGAAACAATTATAAGCCTTGCAGGATTTAAATATGT
GCTTGATGATTATATACTTCATCAGGGCTTAAGCATATGCCAGAGTAATGAAATTAAAGAAAATGAGGCGGTTATAAATA
TAAAAAAAGGATTTGTGATTGTATTTGAAACAAAGGACTAA

Upstream 100 bases:

>100_bases
TTATAGAACTTAACCATAAGTCCTTTATTGTTCAGATATTATAAAATAAAATTGTGAAAAAATAAAGAACATCTGGATTA
ATGTGGTAAAATACGAAAGA

Downstream 100 bases:

>100_bases
AAATGCTTGTAATTATTGGCAGATTGTAATAAAATTAACAAAGAGTTGCGCAATAGCAACGTAAAAAACAAGGGAGGTCT
CGTAATGAGTAATACAACAC

Product: thiamine pyrophosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MILQISKIKEIDMKTLIVTGGSLDISWAKDFVRTINAEYIIAADSGLKYIDKLGLVPDMILGDYDSVEDGLLDKYKSIDI
KTYPKEKDYTDTHIAIINALKAGASVIYILGATGTRMDHTFTNICNMKAALDSDVPCFICDSHNKIYLINDKMGEVKVSK
KGQYGDYVSFVPLSEETIISLAGFKYVLDDYILHQGLSICQSNEIKENEAVINIKKGFVIVFETKD

Sequences:

>Translated_226_residues
MILQISKIKEIDMKTLIVTGGSLDISWAKDFVRTINAEYIIAADSGLKYIDKLGLVPDMILGDYDSVEDGLLDKYKSIDI
KTYPKEKDYTDTHIAIINALKAGASVIYILGATGTRMDHTFTNICNMKAALDSDVPCFICDSHNKIYLINDKMGEVKVSK
KGQYGDYVSFVPLSEETIISLAGFKYVLDDYILHQGLSICQSNEIKENEAVINIKKGFVIVFETKD
>Mature_226_residues
MILQISKIKEIDMKTLIVTGGSLDISWAKDFVRTINAEYIIAADSGLKYIDKLGLVPDMILGDYDSVEDGLLDKYKSIDI
KTYPKEKDYTDTHIAIINALKAGASVIYILGATGTRMDHTFTNICNMKAALDSDVPCFICDSHNKIYLINDKMGEVKVSK
KGQYGDYVSFVPLSEETIISLAGFKYVLDDYILHQGLSICQSNEIKENEAVINIKKGFVIVFETKD

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 25200; Mature: 25200

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILQISKIKEIDMKTLIVTGGSLDISWAKDFVRTINAEYIIAADSGLKYIDKLGLVPDMI
CEEEECCCCCCCEEEEEEECCCEEHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCHHH
LGDYDSVEDGLLDKYKSIDIKTYPKEKDYTDTHIAIINALKAGASVIYILGATGTRMDHT
HCCCCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHH
FTNICNMKAALDSDVPCFICDSHNKIYLINDKMGEVKVSKKGQYGDYVSFVPLSEETIIS
HHHHHHHHHHCCCCCCEEEECCCCEEEEEECCCCEEEEECCCCCCCEEEEEECCHHHEEE
LAGFKYVLDDYILHQGLSICQSNEIKENEAVINIKKGFVIVFETKD
HHHHHHHHHHHHHHHCHHHHCCCCCCCCCEEEEEECCEEEEEEECC
>Mature Secondary Structure
MILQISKIKEIDMKTLIVTGGSLDISWAKDFVRTINAEYIIAADSGLKYIDKLGLVPDMI
CEEEECCCCCCCEEEEEEECCCEEHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCHHH
LGDYDSVEDGLLDKYKSIDIKTYPKEKDYTDTHIAIINALKAGASVIYILGATGTRMDHT
HCCCCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHH
FTNICNMKAALDSDVPCFICDSHNKIYLINDKMGEVKVSKKGQYGDYVSFVPLSEETIIS
HHHHHHHHHHCCCCCCEEEECCCCEEEEEECCCCEEEEECCCCCCCEEEEEECCHHHEEE
LAGFKYVLDDYILHQGLSICQSNEIKENEAVINIKKGFVIVFETKD
HHHHHHHHHHHHHHHCHHHHCCCCCCCCCEEEEEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]