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The map label for this gene is aegA [H]

Identifier: 238916848

GI number: 238916848

Start: 932418

End: 933815

Strand: Direct

Name: aegA [H]

Synonym: EUBELI_00917

Alternate gene names: 238916848

Gene position: 932418-933815 (Clockwise)

Preceding gene: 238916847

Following gene: 238916850

Centisome position: 43.49

GC content: 40.77

Gene sequence:

>1398_bases
ATGGGTTTCAAAATGGACGTAATGAAGAAAGTGCCAGTCAGAGAGCAGGCTCCTGACGTAAGAAATAAGAATTTTGATGA
AGTATGTCTTGGATATAACAAGGAAGAAGCTATGGAAGAGGCAACAAGATGCCTTAACTGTAAGAATGCAAGATGTGTTC
AGGGCTGTCCTGTATCTATCGATATCCCAGCATTTATTCATCAGGTAAAAGAAGGAAATATTGAGGAAGCATATAAGATT
ATTGGTAAGTCAAGTGCACTTCCTGCTGTATGTGGCCGTGTATGTCCACAGGAGACACAGTGTGAAGGTCAGTGTATCAG
AGGAATCAAGGGTGAATCAGTATCAATTGGTAAGCTTGAAAGATTTGTTGCTGACTGGGCAAGAGAGAATAATGTTGAGC
CAGAGAAGCCAACAGAGAAGAAAGGCAAGAAAGTTGCTGTTATCGGTTCAGGTCCTTCAGGACTTACATGTGCCGGAGAC
CTTGCAAAAATGGGATATGACGTAACTATATTCGAGGCTTTACATGAGGCTGGTGGTGTATTAGTATATGGTATTCCTGA
ATTCCGTCTTCCTAAGTCAACAGTAGTTGCCCACGAAGTTGAGAATGTTAAGAAGTTAGGCGTTAAGATTGAGACTAACG
TTGTTATTGGTAAGTCAATGACAATTGACCAGTTACTTGAAGATGAAGGCTTTGATGCAGTATTCATAGGTTCTGGTGCA
GGTCTTCCAAGATTCATGGGTATTCCGGGCGAGAATGCTAACGAAGTATTCTCAGCTAATGAGTACCTTACAAGAAGTAA
TCTTATGAAAGCTTTCAAGGATGAGTATGATACTCCAATAGCAAGATTTAAGAAGGTTGCTATCGTTGGTGGTGGTAATG
TTGCTATGGATGCTGCAAGAACAGCATTAAGACTTGGAGCAGAAACTCATATCGTATACAGAAGAAGTGAGGAGGAGCTT
CCAGCAAGAGCTGAGGAAGTACATCACGCTAAGGAAGAAGGAATTATATTTGACCTTCTTACTAATCCGACAGAGATTCT
TACAGATGACAAGGGTAATGTTACAGGTATGAAGTGTATTAAGATGGAACTTGGTGAACCTGATGCTTCAGGAAGAAGAA
AGCCTGTAGAGATTCCTGGTTCAGAGTTTGTTATGGAATTAGATGCAGTTATCATGTCACTTGGTACTTCACCTAACCCA
CTTATATCTTCAACAACTAAGGGACTTGACATCAACAAGAGAAAATGTATAGTAGCAGAAGAAGAAACTGGTAAAACATC
TAAGGAAGGTGTATATGCCGGTGGAGATGCTGTTACAGGTGCAGCTACAGTTATTCTTGCCATGGGAGCAGGAAAAGCTG
GAGCTAAGGGAATTGATGAATTCCTTTCTAACAACTAA

Upstream 100 bases:

>100_bases
AGAGAAGTGCTATGTACAGAACTGAAGAGGGAAGAGCAATGCTCAAGCTTCAGGAAGGCGATACACATCACGGCGGTTGT
GGACAGTGTAATTAGAAAGG

Downstream 100 bases:

>100_bases
TCAGAATATAGTAAACCCGCTGTGTCTGTTAATTACACAGCGGGTTTTATTGATTATAAGAATTAATTAAACATTTTTTC
AACGAACTGTTCTGAAAAAT

Product: oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MGFKMDVMKKVPVREQAPDVRNKNFDEVCLGYNKEEAMEEATRCLNCKNARCVQGCPVSIDIPAFIHQVKEGNIEEAYKI
IGKSSALPAVCGRVCPQETQCEGQCIRGIKGESVSIGKLERFVADWARENNVEPEKPTEKKGKKVAVIGSGPSGLTCAGD
LAKMGYDVTIFEALHEAGGVLVYGIPEFRLPKSTVVAHEVENVKKLGVKIETNVVIGKSMTIDQLLEDEGFDAVFIGSGA
GLPRFMGIPGENANEVFSANEYLTRSNLMKAFKDEYDTPIARFKKVAIVGGGNVAMDAARTALRLGAETHIVYRRSEEEL
PARAEEVHHAKEEGIIFDLLTNPTEILTDDKGNVTGMKCIKMELGEPDASGRRKPVEIPGSEFVMELDAVIMSLGTSPNP
LISSTTKGLDINKRKCIVAEEETGKTSKEGVYAGGDAVTGAATVILAMGAGKAGAKGIDEFLSNN

Sequences:

>Translated_465_residues
MGFKMDVMKKVPVREQAPDVRNKNFDEVCLGYNKEEAMEEATRCLNCKNARCVQGCPVSIDIPAFIHQVKEGNIEEAYKI
IGKSSALPAVCGRVCPQETQCEGQCIRGIKGESVSIGKLERFVADWARENNVEPEKPTEKKGKKVAVIGSGPSGLTCAGD
LAKMGYDVTIFEALHEAGGVLVYGIPEFRLPKSTVVAHEVENVKKLGVKIETNVVIGKSMTIDQLLEDEGFDAVFIGSGA
GLPRFMGIPGENANEVFSANEYLTRSNLMKAFKDEYDTPIARFKKVAIVGGGNVAMDAARTALRLGAETHIVYRRSEEEL
PARAEEVHHAKEEGIIFDLLTNPTEILTDDKGNVTGMKCIKMELGEPDASGRRKPVEIPGSEFVMELDAVIMSLGTSPNP
LISSTTKGLDINKRKCIVAEEETGKTSKEGVYAGGDAVTGAATVILAMGAGKAGAKGIDEFLSNN
>Mature_464_residues
GFKMDVMKKVPVREQAPDVRNKNFDEVCLGYNKEEAMEEATRCLNCKNARCVQGCPVSIDIPAFIHQVKEGNIEEAYKII
GKSSALPAVCGRVCPQETQCEGQCIRGIKGESVSIGKLERFVADWARENNVEPEKPTEKKGKKVAVIGSGPSGLTCAGDL
AKMGYDVTIFEALHEAGGVLVYGIPEFRLPKSTVVAHEVENVKKLGVKIETNVVIGKSMTIDQLLEDEGFDAVFIGSGAG
LPRFMGIPGENANEVFSANEYLTRSNLMKAFKDEYDTPIARFKKVAIVGGGNVAMDAARTALRLGAETHIVYRRSEEELP
ARAEEVHHAKEEGIIFDLLTNPTEILTDDKGNVTGMKCIKMELGEPDASGRRKPVEIPGSEFVMELDAVIMSLGTSPNPL
ISSTTKGLDINKRKCIVAEEETGKTSKEGVYAGGDAVTGAATVILAMGAGKAGAKGIDEFLSNN

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=474, Percent_Identity=30.379746835443, Blast_Score=190, Evalue=3e-48,
Organism=Escherichia coli, GI1788811, Length=472, Percent_Identity=37.5, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1789606, Length=464, Percent_Identity=35.7758620689655, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI87082180, Length=452, Percent_Identity=36.9469026548673, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1788468, Length=446, Percent_Identity=32.0627802690583, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1789243, Length=435, Percent_Identity=32.8735632183908, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17570289, Length=475, Percent_Identity=30.7368421052632, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71984108, Length=448, Percent_Identity=30.3571428571429, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17543860, Length=162, Percent_Identity=33.9506172839506, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=483, Percent_Identity=28.9855072463768, Blast_Score=183, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6320584, Length=176, Percent_Identity=32.9545454545455, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24665547, Length=480, Percent_Identity=30.625, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24665543, Length=480, Percent_Identity=30.625, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28574881, Length=480, Percent_Identity=30.625, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24665539, Length=480, Percent_Identity=30.625, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24640763, Length=455, Percent_Identity=30.3296703296703, Blast_Score=172, Evalue=6e-43,
Organism=Drosophila melanogaster, GI18858217, Length=455, Percent_Identity=30.3296703296703, Blast_Score=172, Evalue=6e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 50076; Mature: 49945

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKMDVMKKVPVREQAPDVRNKNFDEVCLGYNKEEAMEEATRCLNCKNARCVQGCPVSI
CCCCCCHHHCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCEECCCCCEE
DIPAFIHQVKEGNIEEAYKIIGKSSALPAVCGRVCPQETQCEGQCIRGIKGESVSIGKLE
EHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHH
RFVADWARENNVEPEKPTEKKGKKVAVIGSGPSGLTCAGDLAKMGYDVTIFEALHEAGGV
HHHHHHHHHCCCCCCCCHHHCCCEEEEEECCCCCCEECHHHHHCCCCCHHHHHHHHHCCE
LVYGIPEFRLPKSTVVAHEVENVKKLGVKIETNVVIGKSMTIDQLLEDEGFDAVFIGSGA
EEECCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEEECCCCCHHHHHHCCCCCEEEEECCC
GLPRFMGIPGENANEVFSANEYLTRSNLMKAFKDEYDTPIARFKKVAIVGGGNVAMDAAR
CCCCEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHEEEEEEECCCHHHHHHH
TALRLGAETHIVYRRSEEELPARAEEVHHAKEEGIIFDLLTNPTEILTDDKGNVTGMKCI
HHHHHCCCEEEEEECCCHHCCHHHHHHHHHHHCCEEEEECCCCHHHEECCCCCCCCEEEE
KMELGEPDASGRRKPVEIPGSEFVMELDAVIMSLGTSPNPLISSTTKGLDINKRKCIVAE
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
EETGKTSKEGVYAGGDAVTGAATVILAMGAGKAGAKGIDEFLSNN
CCCCCCCCCCEEECCCCHHHHEEEEEEECCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
GFKMDVMKKVPVREQAPDVRNKNFDEVCLGYNKEEAMEEATRCLNCKNARCVQGCPVSI
CCCCCHHHCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCEECCCCCEE
DIPAFIHQVKEGNIEEAYKIIGKSSALPAVCGRVCPQETQCEGQCIRGIKGESVSIGKLE
EHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHH
RFVADWARENNVEPEKPTEKKGKKVAVIGSGPSGLTCAGDLAKMGYDVTIFEALHEAGGV
HHHHHHHHHCCCCCCCCHHHCCCEEEEEECCCCCCEECHHHHHCCCCCHHHHHHHHHCCE
LVYGIPEFRLPKSTVVAHEVENVKKLGVKIETNVVIGKSMTIDQLLEDEGFDAVFIGSGA
EEECCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEEECCCCCHHHHHHCCCCCEEEEECCC
GLPRFMGIPGENANEVFSANEYLTRSNLMKAFKDEYDTPIARFKKVAIVGGGNVAMDAAR
CCCCEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHEEEEEEECCCHHHHHHH
TALRLGAETHIVYRRSEEELPARAEEVHHAKEEGIIFDLLTNPTEILTDDKGNVTGMKCI
HHHHHCCCEEEEEECCCHHCCHHHHHHHHHHHCCEEEEECCCCHHHEECCCCCCCCEEEE
KMELGEPDASGRRKPVEIPGSEFVMELDAVIMSLGTSPNPLISSTTKGLDINKRKCIVAE
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
EETGKTSKEGVYAGGDAVTGAATVILAMGAGKAGAKGIDEFLSNN
CCCCCCCCCCEEECCCCHHHHEEEEEEECCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]