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The map label for this gene is truB [H]

Identifier: 238916829

GI number: 238916829

Start: 908018

End: 908929

Strand: Direct

Name: truB [H]

Synonym: EUBELI_00897

Alternate gene names: 238916829

Gene position: 908018-908929 (Clockwise)

Preceding gene: 238916828

Following gene: 238916830

Centisome position: 42.35

GC content: 38.49

Gene sequence:

>912_bases
ATGAATGGTGTTTTAAATGTATATAAGGAGCAGGGATTTACATCTCATGATGTAGTTGCAAAGCTTCGGGGAATACTTAA
AACTAAAAAAATTGGTCACACAGGCACACTTGACCCGGATGCGGTCGGGGTGCTTCCTGTGTGCATCGGTAAGGCTACAA
AGCTGTGTGACCTTATAACTGATTGGGGAAAGACTTATGAGGCTGTAATGCTTCTTGGCACAACGACAGATACTCTTGAT
ATATCAGGGAAAATTGTTGCCCAGTCAGAGGTTAATGTGAGTGAAGTGGATGTCCTTAAAGTATGTAATGAATTCATAGG
TGAGTATGAGCAGATACCGCCTATGTATTCAGCTTTAAAGTATAATGGACAGAAGCTTTATGAGCTTGCAAGAAAAGGTA
TTGAGATTGAAAGAAAACCAAGAACTGTCCATATTAATACATTAAGGGTGAATGATATCAATCTTTCGGATAAACAGAAA
ACAGTTACTATTACTGTTGACTGCTCGAAGGGGACTTATATCAGGTCATTATGTGATGATATAGGTAAGAAGCTTGGGTG
TGGAGCATGTATGATGAAGCTTACAAGAACGCGGGTAGGTGAATTCATGCTTGATGATACACTTACACTTAACCAGATAT
CGGCTCTTGTGTTAAAGGGTGAAATAGAAGACAGGATAACTGGCATAGATAAGATATTCAGTGATTATCAGGAGATAAAA
TTTGCAGAAGAATACAGCCGGTATCTTCACAATGGCAATAAGCTGTCAAGAGAACAGATACCTGCAGATGCTGCAATCAG
TGATAATGAGAAATACAGAGTGTACGATTCTGATAAATGCTTTATCGGTGTATATGAATTTCGAGATGAAGTGCTTTATC
CGGTTAAGATATTTTACGAAGACAACAAGTAA

Upstream 100 bases:

>100_bases
GACATGTGAGGGCAGCAGGATTCTCTGCTAAGGGCAATGTCCACAGTATCGTTAATAAGATTGGCGAAATGATTGAGCAG
CAGTATAATGAGGATAATGC

Downstream 100 bases:

>100_bases
AGGGGCGTGATCATCTTTGGAATATATTCATGGAACAGATGATTTTCAATTAAATAAGGAAAGTGCAGTTACATTAGGAA
AATTCGATGGAATTCATACA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNGVLNVYKEQGFTSHDVVAKLRGILKTKKIGHTGTLDPDAVGVLPVCIGKATKLCDLITDWGKTYEAVMLLGTTTDTLD
ISGKIVAQSEVNVSEVDVLKVCNEFIGEYEQIPPMYSALKYNGQKLYELARKGIEIERKPRTVHINTLRVNDINLSDKQK
TVTITVDCSKGTYIRSLCDDIGKKLGCGACMMKLTRTRVGEFMLDDTLTLNQISALVLKGEIEDRITGIDKIFSDYQEIK
FAEEYSRYLHNGNKLSREQIPADAAISDNEKYRVYDSDKCFIGVYEFRDEVLYPVKIFYEDNK

Sequences:

>Translated_303_residues
MNGVLNVYKEQGFTSHDVVAKLRGILKTKKIGHTGTLDPDAVGVLPVCIGKATKLCDLITDWGKTYEAVMLLGTTTDTLD
ISGKIVAQSEVNVSEVDVLKVCNEFIGEYEQIPPMYSALKYNGQKLYELARKGIEIERKPRTVHINTLRVNDINLSDKQK
TVTITVDCSKGTYIRSLCDDIGKKLGCGACMMKLTRTRVGEFMLDDTLTLNQISALVLKGEIEDRITGIDKIFSDYQEIK
FAEEYSRYLHNGNKLSREQIPADAAISDNEKYRVYDSDKCFIGVYEFRDEVLYPVKIFYEDNK
>Mature_303_residues
MNGVLNVYKEQGFTSHDVVAKLRGILKTKKIGHTGTLDPDAVGVLPVCIGKATKLCDLITDWGKTYEAVMLLGTTTDTLD
ISGKIVAQSEVNVSEVDVLKVCNEFIGEYEQIPPMYSALKYNGQKLYELARKGIEIERKPRTVHINTLRVNDINLSDKQK
TVTITVDCSKGTYIRSLCDDIGKKLGCGACMMKLTRTRVGEFMLDDTLTLNQISALVLKGEIEDRITGIDKIFSDYQEIK
FAEEYSRYLHNGNKLSREQIPADAAISDNEKYRVYDSDKCFIGVYEFRDEVLYPVKIFYEDNK

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=224, Percent_Identity=37.0535714285714, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4503337, Length=200, Percent_Identity=29.5, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI215599015, Length=200, Percent_Identity=29.5, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367200, Length=299, Percent_Identity=36.7892976588629, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17553978, Length=199, Percent_Identity=30.1507537688442, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=134, Percent_Identity=43.2835820895522, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=213, Percent_Identity=30.9859154929577, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI281364189, Length=198, Percent_Identity=30.3030303030303, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364187, Length=198, Percent_Identity=30.3030303030303, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364185, Length=198, Percent_Identity=30.3030303030303, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364183, Length=198, Percent_Identity=30.3030303030303, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI62471759, Length=198, Percent_Identity=30.3030303030303, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17975520, Length=198, Percent_Identity=30.3030303030303, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 34158; Mature: 34158

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGVLNVYKEQGFTSHDVVAKLRGILKTKKIGHTGTLDPDAVGVLPVCIGKATKLCDLIT
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHH
DWGKTYEAVMLLGTTTDTLDISGKIVAQSEVNVSEVDVLKVCNEFIGEYEQIPPMYSALK
HHCCCEEEEEEEECCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YNGQKLYELARKGIEIERKPRTVHINTLRVNDINLSDKQKTVTITVDCSKGTYIRSLCDD
CCCHHHHHHHHCCCCEECCCCEEEEEEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHH
IGKKLGCGACMMKLTRTRVGEFMLDDTLTLNQISALVLKGEIEDRITGIDKIFSDYQEIK
HHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCHHHHHHHHHHHHHHHHHHH
FAEEYSRYLHNGNKLSREQIPADAAISDNEKYRVYDSDKCFIGVYEFRDEVLYPVKIFYE
HHHHHHHHHHCCCCCCHHHCCCCCEECCCCCEEEEECCCEEEEEEECCCCEEEEEEEEEE
DNK
CCC
>Mature Secondary Structure
MNGVLNVYKEQGFTSHDVVAKLRGILKTKKIGHTGTLDPDAVGVLPVCIGKATKLCDLIT
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHH
DWGKTYEAVMLLGTTTDTLDISGKIVAQSEVNVSEVDVLKVCNEFIGEYEQIPPMYSALK
HHCCCEEEEEEEECCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YNGQKLYELARKGIEIERKPRTVHINTLRVNDINLSDKQKTVTITVDCSKGTYIRSLCDD
CCCHHHHHHHHCCCCEECCCCEEEEEEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHH
IGKKLGCGACMMKLTRTRVGEFMLDDTLTLNQISALVLKGEIEDRITGIDKIFSDYQEIK
HHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCHHHHHHHHHHHHHHHHHHH
FAEEYSRYLHNGNKLSREQIPADAAISDNEKYRVYDSDKCFIGVYEFRDEVLYPVKIFYE
HHHHHHHHHHCCCCCCHHHCCCCCEECCCCCEEEEECCCEEEEEEECCCCEEEEEEEEEE
DNK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA