Click here to switch to the map view.

The map label for this gene is hisF [H]

Identifier: 238916819

GI number: 238916819

Start: 899217

End: 899975

Strand: Direct

Name: hisF [H]

Synonym: EUBELI_00886

Alternate gene names: 238916819

Gene position: 899217-899975 (Clockwise)

Preceding gene: 238916818

Following gene: 238916820

Centisome position: 41.94

GC content: 39.0

Gene sequence:

>759_bases
ATGTTTACTAAAAGAATTATACCATGCCTTGACTGCAAGGATGGAAGAGTTGTCAAAGGAACTAATTTTGTTGATTTAAG
AGATGCAGGGGATCCTGTAGAGGTTGCATCAATGTACGATAAATCAGGTGCAGACGAACTTGTATTTCTTGATATTACAG
CTTCAAGTGATGGAAGAAACACAACTGTTGATCTTGTAAGAAGAGTGGCAGAAAGAGTGTTTATTCCGTTTACTGTAGGC
GGTGGAATACGAAGCACAGATGATTTTAAGGTACTTTTAAGAGAAGGTGCTGATAAGATATCAATTAATTCGGCAGCTAT
TATGAATCCACAGCTTATATCTGATGCAGCTGATAAGTTTGGAAGCCAGTGTGTTGTAGTTGCAATTGACGCAAAGAGGA
ATAGTGATGGTCATTGGAGCATATATAAGAATGGCGGAAGAGTTGATATGCATATGGATGCTGTAGAATGGGCTATGAAG
GCAGAAAAGCTCGGTGCAGGAGAGATTCTTCTTACAAGTATGGACTGTGACGGAACGAAAGCCGGATATGATATTGAGCT
TACTAATACAATTGCGTCAAATGTATCAATACCTGTAATTGCTTCAGGCGGTGCAGGCAGTAAAGAACATTTTCTTGATG
CATTTACTAAGGGTAAAGCAGATGCAGCACTTGCAGCTTCACTATTCCATTATAAGGAACTTGATATTATGGAGCTTAAG
AAATACCTTGCAGATAATGGTATTCCTATGCGTATGTAG

Upstream 100 bases:

>100_bases
AATGTGTTTGCATGCCAGTTCCATCCTGAAAAGAGCAGTGATGTCGGACTTAAGATATTAAAGAATTTTGCGTCATTATA
ATTGCTAAAAGGAGTTGCTG

Downstream 100 bases:

>100_bases
GAGGATATGGATATGTCAATGATAGATAATGACTGGCTTCCTGCTATTAAGGAGGAATATTCCAAGCCATATTACAAGGA
ATTATTTACATTTGTTAAGA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MFTKRIIPCLDCKDGRVVKGTNFVDLRDAGDPVEVASMYDKSGADELVFLDITASSDGRNTTVDLVRRVAERVFIPFTVG
GGIRSTDDFKVLLREGADKISINSAAIMNPQLISDAADKFGSQCVVVAIDAKRNSDGHWSIYKNGGRVDMHMDAVEWAMK
AEKLGAGEILLTSMDCDGTKAGYDIELTNTIASNVSIPVIASGGAGSKEHFLDAFTKGKADAALAASLFHYKELDIMELK
KYLADNGIPMRM

Sequences:

>Translated_252_residues
MFTKRIIPCLDCKDGRVVKGTNFVDLRDAGDPVEVASMYDKSGADELVFLDITASSDGRNTTVDLVRRVAERVFIPFTVG
GGIRSTDDFKVLLREGADKISINSAAIMNPQLISDAADKFGSQCVVVAIDAKRNSDGHWSIYKNGGRVDMHMDAVEWAMK
AEKLGAGEILLTSMDCDGTKAGYDIELTNTIASNVSIPVIASGGAGSKEHFLDAFTKGKADAALAASLFHYKELDIMELK
KYLADNGIPMRM
>Mature_252_residues
MFTKRIIPCLDCKDGRVVKGTNFVDLRDAGDPVEVASMYDKSGADELVFLDITASSDGRNTTVDLVRRVAERVFIPFTVG
GGIRSTDDFKVLLREGADKISINSAAIMNPQLISDAADKFGSQCVVVAIDAKRNSDGHWSIYKNGGRVDMHMDAVEWAMK
AEKLGAGEILLTSMDCDGTKAGYDIELTNTIASNVSIPVIASGGAGSKEHFLDAFTKGKADAALAASLFHYKELDIMELK
KYLADNGIPMRM

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=48.4496124031008, Blast_Score=233, Evalue=9e-63,
Organism=Escherichia coli, GI87082028, Length=205, Percent_Identity=27.3170731707317, Blast_Score=86, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=33.7579617834395, Blast_Score=161, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27236; Mature: 27236

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTKRIIPCLDCKDGRVVKGTNFVDLRDAGDPVEVASMYDKSGADELVFLDITASSDGRN
CCCCCCCCCCCCCCCEEEECCCEEEECCCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCC
TTVDLVRRVAERVFIPFTVGGGIRSTDDFKVLLREGADKISINSAAIMNPQLISDAADKF
CHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHCCCCEEEECCEEECCCHHHHHHHHHH
GSQCVVVAIDAKRNSDGHWSIYKNGGRVDMHMDAVEWAMKAEKLGAGEILLTSMDCDGTK
CCCEEEEEEECCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCC
AGYDIELTNTIASNVSIPVIASGGAGSKEHFLDAFTKGKADAALAASLFHYKELDIMELK
CCEEEEEHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
KYLADNGIPMRM
HHHHCCCCCCCC
>Mature Secondary Structure
MFTKRIIPCLDCKDGRVVKGTNFVDLRDAGDPVEVASMYDKSGADELVFLDITASSDGRN
CCCCCCCCCCCCCCCEEEECCCEEEECCCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCC
TTVDLVRRVAERVFIPFTVGGGIRSTDDFKVLLREGADKISINSAAIMNPQLISDAADKF
CHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHCCCCEEEECCEEECCCHHHHHHHHHH
GSQCVVVAIDAKRNSDGHWSIYKNGGRVDMHMDAVEWAMKAEKLGAGEILLTSMDCDGTK
CCCEEEEEEECCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCC
AGYDIELTNTIASNVSIPVIASGGAGSKEHFLDAFTKGKADAALAASLFHYKELDIMELK
CCEEEEEHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
KYLADNGIPMRM
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA