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The map label for this gene is hisH [H]

Identifier: 238916818

GI number: 238916818

Start: 898592

End: 899197

Strand: Direct

Name: hisH [H]

Synonym: EUBELI_00885

Alternate gene names: 238916818

Gene position: 898592-899197 (Clockwise)

Preceding gene: 238916817

Following gene: 238916819

Centisome position: 41.91

GC content: 39.44

Gene sequence:

>606_bases
ATGATAGCTATTATTGACTATGACGCAGGTAACTTAAAGAGTGTTGAAAAGGCTCTGGTATCATTAGGAGAGGAAGTTTT
AGTCTCAAGAGATTCAAGTGAAATCTTACAGGCTGATAAGGTTATTCTTCCGGGAGTTGGTTCATTTGGTGATGCAATGA
ACAATCTTGATAAGTTCGGACTAGTTGATACTATTAAGAAGGTGACAGGTAATGGAACCCCATTCCTTGGAATATGTTTG
GGATTACAGCTTTTATTTAAGGAAAGTGATGAGACTCCGGGGGCGGAGGGACTTGATATTCTTCCGGGAAAGATATTAAA
GATTCCTGCTAAGGATGGTTTCAAGATTCCGCATATGGGATGGAATTCTCTTGATATTAAGCCTGAAGCGAGACTTTTTA
AGGGACTTGAAGGTAATCCGTATGTGTATTTTGTACATTCTTATTATCTTAAGGCAGCAGATGAGGGGATTGTGGCAGCA
ACTACGGAGTATACTACACATATTCATGCTTCGGTTGAAAGCGGCAATGTGTTTGCATGCCAGTTCCATCCTGAAAAGAG
CAGTGATGTCGGACTTAAGATATTAAAGAATTTTGCGTCATTATAA

Upstream 100 bases:

>100_bases
TTAAGATATCTGAAGCACAGGGAATATCACTGGCAGATATGTCAAACAGTGATTTTACTAACTATTACTTAAAAATAAAG
GAATACGGAAAGGCAGTACA

Downstream 100 bases:

>100_bases
TTGCTAAAAGGAGTTGCTGATGTTTACTAAAAGAATTATACCATGCCTTGACTGCAAGGATGGAAGAGTTGTCAAAGGAA
CTAATTTTGTTGATTTAAGA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MIAIIDYDAGNLKSVEKALVSLGEEVLVSRDSSEILQADKVILPGVGSFGDAMNNLDKFGLVDTIKKVTGNGTPFLGICL
GLQLLFKESDETPGAEGLDILPGKILKIPAKDGFKIPHMGWNSLDIKPEARLFKGLEGNPYVYFVHSYYLKAADEGIVAA
TTEYTTHIHASVESGNVFACQFHPEKSSDVGLKILKNFASL

Sequences:

>Translated_201_residues
MIAIIDYDAGNLKSVEKALVSLGEEVLVSRDSSEILQADKVILPGVGSFGDAMNNLDKFGLVDTIKKVTGNGTPFLGICL
GLQLLFKESDETPGAEGLDILPGKILKIPAKDGFKIPHMGWNSLDIKPEARLFKGLEGNPYVYFVHSYYLKAADEGIVAA
TTEYTTHIHASVESGNVFACQFHPEKSSDVGLKILKNFASL
>Mature_201_residues
MIAIIDYDAGNLKSVEKALVSLGEEVLVSRDSSEILQADKVILPGVGSFGDAMNNLDKFGLVDTIKKVTGNGTPFLGICL
GLQLLFKESDETPGAEGLDILPGKILKIPAKDGFKIPHMGWNSLDIKPEARLFKGLEGNPYVYFVHSYYLKAADEGIVAA
TTEYTTHIHASVESGNVFACQFHPEKSSDVGLKILKNFASL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=41, Blast_Score=143, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=34.9282296650718, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21701; Mature: 21701

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIIDYDAGNLKSVEKALVSLGEEVLVSRDSSEILQADKVILPGVGSFGDAMNNLDKFG
CEEEEECCCCCHHHHHHHHHHCCHHHEECCCCHHHHHHCCEEECCCCCHHHHHHHHHHCC
LVDTIKKVTGNGTPFLGICLGLQLLFKESDETPGAEGLDILPGKILKIPAKDGFKIPHMG
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEECCCCEEEECCCCCCCCCCCC
WNSLDIKPEARLFKGLEGNPYVYFVHSYYLKAADEGIVAATTEYTTHIHASVESGNVFAC
CCCCCCCCHHHHHCCCCCCCEEEEEEEEEEHHCCCCEEEEEEEEEEEEEEEECCCCEEEE
QFHPEKSSDVGLKILKNFASL
EECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MIAIIDYDAGNLKSVEKALVSLGEEVLVSRDSSEILQADKVILPGVGSFGDAMNNLDKFG
CEEEEECCCCCHHHHHHHHHHCCHHHEECCCCHHHHHHCCEEECCCCCHHHHHHHHHHCC
LVDTIKKVTGNGTPFLGICLGLQLLFKESDETPGAEGLDILPGKILKIPAKDGFKIPHMG
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEECCCCEEEECCCCCCCCCCCC
WNSLDIKPEARLFKGLEGNPYVYFVHSYYLKAADEGIVAATTEYTTHIHASVESGNVFAC
CCCCCCCCHHHHHCCCCCCCEEEEEEEEEEHHCCCCEEEEEEEEEEEEEEEECCCCEEEE
QFHPEKSSDVGLKILKNFASL
EECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA