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The map label for this gene is leuB [H]

Identifier: 238916699

GI number: 238916699

Start: 770719

End: 771801

Strand: Direct

Name: leuB [H]

Synonym: EUBELI_00761

Alternate gene names: 238916699

Gene position: 770719-771801 (Clockwise)

Preceding gene: 238916698

Following gene: 238916700

Centisome position: 35.94

GC content: 39.52

Gene sequence:

>1083_bases
ATGGAAAGCAGAATAACTTTAATTCCAGGTGATGGAATAGGACCAGAAATCGTCAGAGAGGCTGTGAAAGTGCTTGACAG
TGTGGCTGACAAATACGGTCACAAGTTCGATTATACCAAGATTCTTATGGGTGGATGCTCAATTGATGAGTATGGCGTTC
CACTTACAGATGAAGCAGTAGCAACAGCTAAAGCATCTGATGCTGTACTTCTTGGAGCAGTTGGAGGCAATGTGGGTAAT
TCCAAGTGGTATGATGTTGCACCTAATTTAAGACCAGAAGCGGGACTTTTAAAGATTCGTAAGGAATTAGGACTTTTTGC
TAATTTACGTCCGGCATATCTTTATGATGAACTTAAGGCAGCGTGTCCATTGAAGGAAGAAATAATCGGTGATGGATTTG
ATATGGTAATTATGAGAGAGCTTACAGGCGGCCTTTACTTTGGTAATAGATATACTAAGGAAATTGACGGATTGGAGACA
GCCGTTGATACACTTACATACAACGAAGAAGAAATCAGAAGAATTGCAATTAAGGGATTTGAGATTGCAATGAAGAGAAG
AAAGAAAGTAACAAGCGTTGATAAGGCAAATGTTCTTGATTCTTCAAGACTCTGGAGAAAGATTGTACATGAGGTTGCAA
AGGATTATCCGGAAGTTGAAGTTTCAGACATGCTTGTAGATAACTGTGCTATGCAGCTTGTTATGAATCCTGGACAGTTC
GATGTTATTCTTACTGAGAACATGTTTGGAGACATATTATCAGATGAGGCAAGTATGATAACAGGTTCAATCGGAATGTT
ATCAAGTGCAAGCCTTAATAAGACTAAGTTAGGTCTTTATGAGCCAAGCCATGGTTCAGCACCTGATATTGCAGGAAAGA
ATGTTGCTAATCCAATTGCTACAATTCTCAGTGCAGCCATGATGTTAAGATATTCACTTGATCTTGACAAGGAAGCAGAT
GCTATTGAAGCAGCAGTTGCAACTGTTTTAAAGGAAGGCTACAGAACAACTGATATTATGTCAGAAGGATGCACACTTGT
AGGAACTGATAAGATGGGCGACTTAATCGCAGAGAGAGTTTAA

Upstream 100 bases:

>100_bases
ACACATTTGTCTGGTCAGGAATCCTTTTTTTATTGAAAATGTTGTGCAAATGTTATATCATATTAAATTGAATTATCATA
TGAAAAAGGGGTAAAGGTTT

Downstream 100 bases:

>100_bases
TACATTTAAATAATACATATTAAGGAGATAAGCTATGATAAGTGATAACGCCAGAAGTGGTATGCAGCAGGCACCTGCAC
GAAGCCTTTTTAATGCACTT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MESRITLIPGDGIGPEIVREAVKVLDSVADKYGHKFDYTKILMGGCSIDEYGVPLTDEAVATAKASDAVLLGAVGGNVGN
SKWYDVAPNLRPEAGLLKIRKELGLFANLRPAYLYDELKAACPLKEEIIGDGFDMVIMRELTGGLYFGNRYTKEIDGLET
AVDTLTYNEEEIRRIAIKGFEIAMKRRKKVTSVDKANVLDSSRLWRKIVHEVAKDYPEVEVSDMLVDNCAMQLVMNPGQF
DVILTENMFGDILSDEASMITGSIGMLSSASLNKTKLGLYEPSHGSAPDIAGKNVANPIATILSAAMMLRYSLDLDKEAD
AIEAAVATVLKEGYRTTDIMSEGCTLVGTDKMGDLIAERV

Sequences:

>Translated_360_residues
MESRITLIPGDGIGPEIVREAVKVLDSVADKYGHKFDYTKILMGGCSIDEYGVPLTDEAVATAKASDAVLLGAVGGNVGN
SKWYDVAPNLRPEAGLLKIRKELGLFANLRPAYLYDELKAACPLKEEIIGDGFDMVIMRELTGGLYFGNRYTKEIDGLET
AVDTLTYNEEEIRRIAIKGFEIAMKRRKKVTSVDKANVLDSSRLWRKIVHEVAKDYPEVEVSDMLVDNCAMQLVMNPGQF
DVILTENMFGDILSDEASMITGSIGMLSSASLNKTKLGLYEPSHGSAPDIAGKNVANPIATILSAAMMLRYSLDLDKEAD
AIEAAVATVLKEGYRTTDIMSEGCTLVGTDKMGDLIAERV
>Mature_360_residues
MESRITLIPGDGIGPEIVREAVKVLDSVADKYGHKFDYTKILMGGCSIDEYGVPLTDEAVATAKASDAVLLGAVGGNVGN
SKWYDVAPNLRPEAGLLKIRKELGLFANLRPAYLYDELKAACPLKEEIIGDGFDMVIMRELTGGLYFGNRYTKEIDGLET
AVDTLTYNEEEIRRIAIKGFEIAMKRRKKVTSVDKANVLDSSRLWRKIVHEVAKDYPEVEVSDMLVDNCAMQLVMNPGQF
DVILTENMFGDILSDEASMITGSIGMLSSASLNKTKLGLYEPSHGSAPDIAGKNVANPIATILSAAMMLRYSLDLDKEAD
AIEAAVATVLKEGYRTTDIMSEGCTLVGTDKMGDLIAERV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=336, Percent_Identity=33.3333333333333, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI4758582, Length=344, Percent_Identity=29.6511627906977, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI28178816, Length=346, Percent_Identity=27.1676300578035, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI28178821, Length=346, Percent_Identity=27.1676300578035, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI28178838, Length=324, Percent_Identity=29.320987654321, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI28178819, Length=170, Percent_Identity=35.2941176470588, Blast_Score=102, Evalue=8e-22,
Organism=Escherichia coli, GI87081683, Length=359, Percent_Identity=54.3175487465181, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1788101, Length=365, Percent_Identity=35.6164383561644, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1787381, Length=406, Percent_Identity=25.3694581280788, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71986051, Length=337, Percent_Identity=32.9376854599407, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17550882, Length=341, Percent_Identity=31.6715542521994, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25144293, Length=360, Percent_Identity=31.1111111111111, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17505779, Length=350, Percent_Identity=30.2857142857143, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=368, Percent_Identity=49.7282608695652, Blast_Score=305, Evalue=9e-84,
Organism=Saccharomyces cerevisiae, GI6322097, Length=355, Percent_Identity=36.6197183098592, Blast_Score=179, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6324291, Length=337, Percent_Identity=31.7507418397626, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6324709, Length=350, Percent_Identity=30.2857142857143, Blast_Score=133, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24643270, Length=339, Percent_Identity=33.9233038348083, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24643268, Length=339, Percent_Identity=33.9233038348083, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24661184, Length=347, Percent_Identity=31.4121037463977, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI161078635, Length=359, Percent_Identity=30.3621169916435, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI161078633, Length=359, Percent_Identity=30.3621169916435, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24650122, Length=359, Percent_Identity=30.3621169916435, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI161078637, Length=359, Percent_Identity=30.3621169916435, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI161078639, Length=357, Percent_Identity=30.2521008403361, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI281362242, Length=348, Percent_Identity=29.5977011494253, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24648872, Length=348, Percent_Identity=29.5977011494253, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI20130355, Length=342, Percent_Identity=26.3157894736842, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39193; Mature: 39193

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESRITLIPGDGIGPEIVREAVKVLDSVADKYGHKFDYTKILMGGCSIDEYGVPLTDEAV
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCCCCCCCHHH
ATAKASDAVLLGAVGGNVGNSKWYDVAPNLRPEAGLLKIRKELGLFANLRPAYLYDELKA
HHCCCCCEEEEEECCCCCCCCCEEECCCCCCCCHHHHHHHHHHCHHHCCCHHHHHHHHHH
ACPLKEEIIGDGFDMVIMRELTGGLYFGNRYTKEIDGLETAVDTLTYNEEEIRRIAIKGF
HCCCHHHHHCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EIAMKRRKKVTSVDKANVLDSSRLWRKIVHEVAKDYPEVEVSDMLVDNCAMQLVMNPGQF
HHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCCE
DVILTENMFGDILSDEASMITGSIGMLSSASLNKTKLGLYEPSHGSAPDIAGKNVANPIA
EEEEECCHHHHHHCCCHHHHCCCHHHCCCCCCCCCEECEECCCCCCCCCCCCCCHHHHHH
TILSAAMMLRYSLDLDKEADAIEAAVATVLKEGYRTTDIMSEGCTLVGTDKMGDLIAERV
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCHHHHHHHHCC
>Mature Secondary Structure
MESRITLIPGDGIGPEIVREAVKVLDSVADKYGHKFDYTKILMGGCSIDEYGVPLTDEAV
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCCCCCCCHHH
ATAKASDAVLLGAVGGNVGNSKWYDVAPNLRPEAGLLKIRKELGLFANLRPAYLYDELKA
HHCCCCCEEEEEECCCCCCCCCEEECCCCCCCCHHHHHHHHHHCHHHCCCHHHHHHHHHH
ACPLKEEIIGDGFDMVIMRELTGGLYFGNRYTKEIDGLETAVDTLTYNEEEIRRIAIKGF
HCCCHHHHHCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EIAMKRRKKVTSVDKANVLDSSRLWRKIVHEVAKDYPEVEVSDMLVDNCAMQLVMNPGQF
HHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCCE
DVILTENMFGDILSDEASMITGSIGMLSSASLNKTKLGLYEPSHGSAPDIAGKNVANPIA
EEEEECCHHHHHHCCCHHHHCCCHHHCCCCCCCCCEECEECCCCCCCCCCCCCCHHHHHH
TILSAAMMLRYSLDLDKEADAIEAAVATVLKEGYRTTDIMSEGCTLVGTDKMGDLIAERV
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA