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The map label for this gene is hemC

Identifier: 238916686

GI number: 238916686

Start: 758539

End: 759426

Strand: Direct

Name: hemC

Synonym: EUBELI_00748

Alternate gene names: 238916686

Gene position: 758539-759426 (Clockwise)

Preceding gene: 238916685

Following gene: 238916687

Centisome position: 35.38

GC content: 45.72

Gene sequence:

>888_bases
ATGCATTACAGAATAGGAACGAGAGGTTCGAAGCTGGCACTTGTCCAGTCGGAATATGTTAAGAGAAGAATGGAAGAGGC
GTATCCTGAGGATACATTTGAACTTGTTATAATTAAGACAACAGGCGACAAGGTTACGGATAAGCCACTTGCTGCGATTG
GAACTAAGGGGTTTTTTGTAAAGGAGATTGAGGAAGCGCTTCTTTCAGGCAGCATTGATATGGCGGTTCACAGCATGAAG
GATATGCCGGCTGAATGTGCGGCTGGACTTACATTTGCAAAGGCGTGGAAACGAGAGGATTGCAGAGATGTACTTATATT
AAAGACTGCAGGAAGTTTTTCAGAACTTCCTTCTGGTGCGGTTATAGGTACTGGGAGCCTCAGGCGTGCATGCCAGCTTG
CAATGCTGCGTCCTGATATCCAGTTTACTGCTATCAGGGGAAATGTTGATACAAGAATTAACAAACTTATGGACGATTCA
TACGGACTTGATGGAATTGTGCTTGCGGCAGCAGGACTTAACAGACTTGGCAGATCATCTGAGATAACAGAGTATCTTGA
CCCGGAGGTCGTTATTCCGGCACCGGCACAGGGAGTTCTTGCGATTGAGACTGCAGAGGTGAATACAGAACTTCTTGATA
AGATAAATGCTTTGTCAGATGATAATTCTGATAGAGAAGCTGTTGCTGAAAGAACATTTTTAAGATTAACAGGCGGCGGC
TGTCATGCCCCGGTTGGCGCACACTGTGTGACAAAGGATAACGGTGACCTTCGAATGGTTGTGTTATTTGGTAATGATGA
TTGCAGCAGGATTCTTAGAATTGAGGTTACAGGAACTGACAGCGAGGCGGTTGGCCACGAAGCAGCCCGTATGCTTGGAT
TGGAGTAG

Upstream 100 bases:

>100_bases
TGTTAAAAGAACCGATTGAAGAATTGAAAAATGTGGAAGAAAATGAACAGCAGCAGTATAAAGACATGGTGGAGAGACTG
TTTGGCTTGTAGGGAGGATT

Downstream 100 bases:

>100_bases
ATTGTGGGTAAATACATATATACATATATTGAAAACGGACAGATGTACGATAAAAGCCCTTCCTTTGCAGAATTACTTGC
AAAGGAGGGGCTTTCCTGCG

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MHYRIGTRGSKLALVQSEYVKRRMEEAYPEDTFELVIIKTTGDKVTDKPLAAIGTKGFFVKEIEEALLSGSIDMAVHSMK
DMPAECAAGLTFAKAWKREDCRDVLILKTAGSFSELPSGAVIGTGSLRRACQLAMLRPDIQFTAIRGNVDTRINKLMDDS
YGLDGIVLAAAGLNRLGRSSEITEYLDPEVVIPAPAQGVLAIETAEVNTELLDKINALSDDNSDREAVAERTFLRLTGGG
CHAPVGAHCVTKDNGDLRMVVLFGNDDCSRILRIEVTGTDSEAVGHEAARMLGLE

Sequences:

>Translated_295_residues
MHYRIGTRGSKLALVQSEYVKRRMEEAYPEDTFELVIIKTTGDKVTDKPLAAIGTKGFFVKEIEEALLSGSIDMAVHSMK
DMPAECAAGLTFAKAWKREDCRDVLILKTAGSFSELPSGAVIGTGSLRRACQLAMLRPDIQFTAIRGNVDTRINKLMDDS
YGLDGIVLAAAGLNRLGRSSEITEYLDPEVVIPAPAQGVLAIETAEVNTELLDKINALSDDNSDREAVAERTFLRLTGGG
CHAPVGAHCVTKDNGDLRMVVLFGNDDCSRILRIEVTGTDSEAVGHEAARMLGLE
>Mature_295_residues
MHYRIGTRGSKLALVQSEYVKRRMEEAYPEDTFELVIIKTTGDKVTDKPLAAIGTKGFFVKEIEEALLSGSIDMAVHSMK
DMPAECAAGLTFAKAWKREDCRDVLILKTAGSFSELPSGAVIGTGSLRRACQLAMLRPDIQFTAIRGNVDTRINKLMDDS
YGLDGIVLAAAGLNRLGRSSEITEYLDPEVVIPAPAQGVLAIETAEVNTELLDKINALSDDNSDREAVAERTFLRLTGGG
CHAPVGAHCVTKDNGDLRMVVLFGNDDCSRILRIEVTGTDSEAVGHEAARMLGLE

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI66933009, Length=253, Percent_Identity=39.1304347826087, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI20149500, Length=253, Percent_Identity=39.1304347826087, Blast_Score=191, Evalue=5e-49,
Organism=Escherichia coli, GI48994974, Length=284, Percent_Identity=42.6056338028169, Blast_Score=209, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6319996, Length=285, Percent_Identity=33.3333333333333, Blast_Score=168, Evalue=9e-43,
Organism=Drosophila melanogaster, GI20130425, Length=245, Percent_Identity=39.5918367346939, Blast_Score=187, Evalue=9e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_EUBE2 (C4Z552)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930203.1
- GeneID:   7957293
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00748
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 31917; Mature: 31917

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYRIGTRGSKLALVQSEYVKRRMEEAYPEDTFELVIIKTTGDKVTDKPLAAIGTKGFFV
CCEECCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHCCCCCHH
KEIEEALLSGSIDMAVHSMKDMPAECAAGLTFAKAWKREDCRDVLILKTAGSFSELPSGA
HHHHHHHHCCCHHHHHHHHHCCCHHHHHCCHHHHHHCCCCCCEEEEEEECCCHHHCCCCC
VIGTGSLRRACQLAMLRPDIQFTAIRGNVDTRINKLMDDSYGLDGIVLAAAGLNRLGRSS
EEECHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCCCCEEEEHHHHHHCCCCC
EITEYLDPEVVIPAPAQGVLAIETAEVNTELLDKINALSDDNSDREAVAERTFLRLTGGG
CHHHHCCCCEEECCCCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHEEEEECCC
CHAPVGAHCVTKDNGDLRMVVLFGNDDCSRILRIEVTGTDSEAVGHEAARMLGLE
CCCCCCCEEEEECCCCEEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MHYRIGTRGSKLALVQSEYVKRRMEEAYPEDTFELVIIKTTGDKVTDKPLAAIGTKGFFV
CCEECCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHCCCCCHH
KEIEEALLSGSIDMAVHSMKDMPAECAAGLTFAKAWKREDCRDVLILKTAGSFSELPSGA
HHHHHHHHCCCHHHHHHHHHCCCHHHHHCCHHHHHHCCCCCCEEEEEEECCCHHHCCCCC
VIGTGSLRRACQLAMLRPDIQFTAIRGNVDTRINKLMDDSYGLDGIVLAAAGLNRLGRSS
EEECHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCCCCEEEEHHHHHHCCCCC
EITEYLDPEVVIPAPAQGVLAIETAEVNTELLDKINALSDDNSDREAVAERTFLRLTGGG
CHHHHCCCCEEECCCCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHEEEEECCC
CHAPVGAHCVTKDNGDLRMVVLFGNDDCSRILRIEVTGTDSEAVGHEAARMLGLE
CCCCCCCEEEEECCCCEEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA