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The map label for this gene is thiC [H]

Identifier: 238916649

GI number: 238916649

Start: 710747

End: 712093

Strand: Direct

Name: thiC [H]

Synonym: EUBELI_00711

Alternate gene names: 238916649

Gene position: 710747-712093 (Clockwise)

Preceding gene: 238916648

Following gene: 238916650

Centisome position: 33.15

GC content: 39.87

Gene sequence:

>1347_bases
ATGATTTTCCTGTTAATTATCCAGAATGGAGGAATACATATGGAATACGCAACACAGATGGATGCTGCAAGAAAAGGCAT
TGTTACTAAAGAAATGGAAATTGTTGCAAAGAAAGAAAATATAGATGTCGAGAGTTTAAGACAGCTTATAGCTAAGGGAC
AGGTTATCATTCCTGCCAATAAGAATCATAAATGTCTTGACCCTAACGGTATCGGTAATAAGTTAAGAACTAAGATTAAT
GTTAATCTTGGAACTTCAAGAGACTGCGTGGATCTTGATATGGAGCTGGACAAGGTTAATAATGCTGTAAAGATGGGAGC
AGAAGCTATCATGGATCTTTCTTCTTTCGGTGATACACAGAAATTCAGAAGAAAGCTGACAGCAGAATGTCCTGCAACAA
TAGGAACAGTTCCTATATATGATGCAGTTGTATATTATCATAAGGCACTTAAGGATATCACAGCACAGGAGTGGCTTGAT
GTTGTAGAAATGCATGCTAAAGACGGCGTTGATTTCATGACTATACATTGTGGTATCAACAAGGCTACAGCTAAGAAGTT
CAGAGCAGATAAGAGACTTATGAATATTGTATCAAGAGGCGGTTCTATCATATATGCATGGATGGAGATGACAGGAAATG
AGAATCCGTTCTTTGAATATTATGACAGAGTGCTTGAGATATGCCGAGAATATGATGTTACAATGAGTCTTGGAGATGCG
TGCAGACCGGGATGTATTATGGATGCATCAGATATCTCACAGATTGAAGAGCTTGTTACACTTGGCGAGCTTACAAGAAG
AGCATGGGAGAAGGATGTCCAGGTTATGGTTGAAGGACCAGGACATATGCCTCTTAACCAGATTAAGGCTAATATGGAGA
TTCAGCAGACAATATGCAAGGGAGCACCTTTCTATGTATTAGGACCTCTTGTTACTGATATAGCTCCAGGATATGATCAC
ATTACAGCAGCAATCGGTGGAGCTATCGCAGCTACTTACGGAGCTTCATTCCTCTGCTATGTAACACCTGCAGAACATTT
AAGATTACCAGATCTTAATGATGTTAAGGAAGGAATTATTGCTTCTAAGATTGCAGCACATGCGGCTGATATTGCAAAGG
GCGTACCGGGTGCAATGGACTGGGATTATAAGATGGACAAGGCAAGAAAGGTTCTTGACTGGGAAGCAATGTTTGACGTT
GCGATTGACCCTGAGAAGGCAAGAGCTTACAGAGCTTCATCTAAGCCTGAGAAGGAAGATACATGTTCTATGTGTGGTAA
TTTCTGTGCAGTAAAGAATATGAACAAGATTATGGAAGGCGAGATTGTAAGTATATTTGATGAGTAA

Upstream 100 bases:

>100_bases
CCGACCCATAACCTGATGTGGATAATGCCACCGTAGGGATTCTTTAGAGGGCTTTTAGCTCTTCATTTGATTGTTTCGAA
ATCGGATTTCCACAAGGCTT

Downstream 100 bases:

>100_bases
TAGCATATATGAAAGGGGTTGCCATGGTGGTGGCAGCTCTTTTTTTATATAAAGAATGTGATTTGCCATGAAACTGGATA
AATGTTATAATTAAAAAATG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MIFLLIIQNGGIHMEYATQMDAARKGIVTKEMEIVAKKENIDVESLRQLIAKGQVIIPANKNHKCLDPNGIGNKLRTKIN
VNLGTSRDCVDLDMELDKVNNAVKMGAEAIMDLSSFGDTQKFRRKLTAECPATIGTVPIYDAVVYYHKALKDITAQEWLD
VVEMHAKDGVDFMTIHCGINKATAKKFRADKRLMNIVSRGGSIIYAWMEMTGNENPFFEYYDRVLEICREYDVTMSLGDA
CRPGCIMDASDISQIEELVTLGELTRRAWEKDVQVMVEGPGHMPLNQIKANMEIQQTICKGAPFYVLGPLVTDIAPGYDH
ITAAIGGAIAATYGASFLCYVTPAEHLRLPDLNDVKEGIIASKIAAHAADIAKGVPGAMDWDYKMDKARKVLDWEAMFDV
AIDPEKARAYRASSKPEKEDTCSMCGNFCAVKNMNKIMEGEIVSIFDE

Sequences:

>Translated_448_residues
MIFLLIIQNGGIHMEYATQMDAARKGIVTKEMEIVAKKENIDVESLRQLIAKGQVIIPANKNHKCLDPNGIGNKLRTKIN
VNLGTSRDCVDLDMELDKVNNAVKMGAEAIMDLSSFGDTQKFRRKLTAECPATIGTVPIYDAVVYYHKALKDITAQEWLD
VVEMHAKDGVDFMTIHCGINKATAKKFRADKRLMNIVSRGGSIIYAWMEMTGNENPFFEYYDRVLEICREYDVTMSLGDA
CRPGCIMDASDISQIEELVTLGELTRRAWEKDVQVMVEGPGHMPLNQIKANMEIQQTICKGAPFYVLGPLVTDIAPGYDH
ITAAIGGAIAATYGASFLCYVTPAEHLRLPDLNDVKEGIIASKIAAHAADIAKGVPGAMDWDYKMDKARKVLDWEAMFDV
AIDPEKARAYRASSKPEKEDTCSMCGNFCAVKNMNKIMEGEIVSIFDE
>Mature_448_residues
MIFLLIIQNGGIHMEYATQMDAARKGIVTKEMEIVAKKENIDVESLRQLIAKGQVIIPANKNHKCLDPNGIGNKLRTKIN
VNLGTSRDCVDLDMELDKVNNAVKMGAEAIMDLSSFGDTQKFRRKLTAECPATIGTVPIYDAVVYYHKALKDITAQEWLD
VVEMHAKDGVDFMTIHCGINKATAKKFRADKRLMNIVSRGGSIIYAWMEMTGNENPFFEYYDRVLEICREYDVTMSLGDA
CRPGCIMDASDISQIEELVTLGELTRRAWEKDVQVMVEGPGHMPLNQIKANMEIQQTICKGAPFYVLGPLVTDIAPGYDH
ITAAIGGAIAATYGASFLCYVTPAEHLRLPDLNDVKEGIIASKIAAHAADIAKGVPGAMDWDYKMDKARKVLDWEAMFDV
AIDPEKARAYRASSKPEKEDTCSMCGNFCAVKNMNKIMEGEIVSIFDE

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=447, Percent_Identity=43.8478747203579, Blast_Score=366, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 49714; Mature: 49714

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFLLIIQNGGIHMEYATQMDAARKGIVTKEMEIVAKKENIDVESLRQLIAKGQVIIPAN
CEEEEEEECCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECC
KNHKCLDPNGIGNKLRTKINVNLGTSRDCVDLDMELDKVNNAVKMGAEAIMDLSSFGDTQ
CCCCCCCCCCCCCCEEEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCHH
KFRRKLTAECPATIGTVPIYDAVVYYHKALKDITAQEWLDVVEMHAKDGVDFMTIHCGIN
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
KATAKKFRADKRLMNIVSRGGSIIYAWMEMTGNENPFFEYYDRVLEICREYDVTMSLGDA
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCC
CRPGCIMDASDISQIEELVTLGELTRRAWEKDVQVMVEGPGHMPLNQIKANMEIQQTICK
CCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHCCHHHHHHHHC
GAPFYVLGPLVTDIAPGYDHITAAIGGAIAATYGASFLCYVTPAEHLRLPDLNDVKEGII
CCCCEEEHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHH
ASKIAAHAADIAKGVPGAMDWDYKMDKARKVLDWEAMFDVAIDPEKARAYRASSKPEKED
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEEEECHHHHHHHCCCCCCCCHH
TCSMCGNFCAVKNMNKIMEGEIVSIFDE
HHHHHHCHHHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure
MIFLLIIQNGGIHMEYATQMDAARKGIVTKEMEIVAKKENIDVESLRQLIAKGQVIIPAN
CEEEEEEECCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECC
KNHKCLDPNGIGNKLRTKINVNLGTSRDCVDLDMELDKVNNAVKMGAEAIMDLSSFGDTQ
CCCCCCCCCCCCCCEEEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCHH
KFRRKLTAECPATIGTVPIYDAVVYYHKALKDITAQEWLDVVEMHAKDGVDFMTIHCGIN
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
KATAKKFRADKRLMNIVSRGGSIIYAWMEMTGNENPFFEYYDRVLEICREYDVTMSLGDA
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCC
CRPGCIMDASDISQIEELVTLGELTRRAWEKDVQVMVEGPGHMPLNQIKANMEIQQTICK
CCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHCCHHHHHHHHC
GAPFYVLGPLVTDIAPGYDHITAAIGGAIAATYGASFLCYVTPAEHLRLPDLNDVKEGII
CCCCEEEHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHH
ASKIAAHAADIAKGVPGAMDWDYKMDKARKVLDWEAMFDVAIDPEKARAYRASSKPEKED
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEEEECHHHHHHHCCCCCCCCHH
TCSMCGNFCAVKNMNKIMEGEIVSIFDE
HHHHHHCHHHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA