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The map label for this gene is radA [H]

Identifier: 238916636

GI number: 238916636

Start: 694953

End: 696332

Strand: Direct

Name: radA [H]

Synonym: EUBELI_00693

Alternate gene names: 238916636

Gene position: 694953-696332 (Clockwise)

Preceding gene: 238916635

Following gene: 238916637

Centisome position: 32.41

GC content: 40.72

Gene sequence:

>1380_bases
ATGGCAAAGGCAAAGAATACAGCTTTTTTTTGTAAAGAATGTGGATATGAGTCTGCTAAATGGTTCGGGCAGTGTCCGGC
GTGTAAGGAATGGAACACATTTGTAGAAGAACCAGTAAGTAAACCTAAGTCAGCCAAAGGAATAGCAGGCACAAGCCAGA
GGGCAGCGGCATCATCAGCGCCTGTTCCTGTAGAGATAAATAGTGTAAGTTTTGAAGAAAATGACAGAACATCCACAGGC
ATAGCTGAACTAGACAGGGTACTTGGCGGAGGAATTGTTGCTGGTTCTTTAGTGCTTGTAGGAGGAGATCCTGGAATAGG
AAAGTCAACACTTCTGCTTCAGATGTGTTACAACCTGTCAGCAGAAGGAAAAGATGTACTTTATATATCCGGTGAAGAAT
CGCTTAAGCAGATAAAGCTTAGGGCAGACAGAATTGGCAAATGCAGTGGAACACTTAAGTTATTATGTGAGACAAGCTTA
GATAGCATAGAAGATATTCTTACAAGAAATAAGCCTGAGATTGTGATAATTGATTCGATACAGACTATGTATAAAGAAGA
TGTGGCAGCAGCACCGGGAAGTGTTTCTCAGGTAAGAGAGACAACATCCATACTTATGCAGCTTGCGAAGGGACTGAACA
TTACTATATTCATTGTAGGACATGTTACCAAGGAAGGAACAGTTGCCGGACCAAGAATGCTTGAGCATATGGTTGATACG
GTACTTTATTTTGAAGGTGACAGATATGCTTCATACAGGATATTAAGAGGCGTAAAAAACAGATTTGGCTCAACTAATGA
AATTGGTGTATTTGAAATGCAGCAGAGCGGTCTGTCGGAAGTTGAGAATCCATCGGAATATATGCTCAATGGAAGACCAG
AGAATGCATCAGGTTCAGTTGTTGTGTGCCTTTTGGAAGGAACACGCCCTATAATGGTAGAGATTCAGGCATTGGTGTGC
GATTCTAATTTTGGAATGGCAAGAAGGACTGCAGCAGGAACTGACTATAACAGAGTTAATCTTCTTATGGCTGTTCTTGA
AAAGAGAGCGGGTATACATATGTCAGGCAGTGATGCTTATGTGAATGTTGCAGGTGGAATTAAAGTAAGTGAGCCAGCGC
TTGATTTGGGAATTGTGATGGCCCTTGTGAGCAGCTTTAAGAACAAGGCAATAGACGAAAAGACAGTTATATTTGGTGAA
GTTGGACTTGCAGGTGAGGTGAGAGCTGTAAGCCAGGCACAACAGAGAGTTAATGAAGCTGTTAAGCTTGGTTTTAAAAC
ATGTATATTGCCGGGTGTATGCTTGAAAAACATAAAGAAGAATGATAAAATTGAAATAATTGGTGTTAATAATGTTCAGG
AAGCAATTAAACTGATATAA

Upstream 100 bases:

>100_bases
AAGTAGATATTAAGAAGGCTTGATTATAATTAAAAGTATTTTGATTTTACAGGGCTGCTACACATTTATATGTTGTGGCA
GCCTTTTTTAGGGGGGATAT

Downstream 100 bases:

>100_bases
AATACATAGGATGGGGGATTACTTATGAGTAACGAAGCAATGGTGAATAATCTCACCAATATTCTTAATGGACTTGATGA
TTCACAGGAAAAGCTTGAAA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MAKAKNTAFFCKECGYESAKWFGQCPACKEWNTFVEEPVSKPKSAKGIAGTSQRAAASSAPVPVEINSVSFEENDRTSTG
IAELDRVLGGGIVAGSLVLVGGDPGIGKSTLLLQMCYNLSAEGKDVLYISGEESLKQIKLRADRIGKCSGTLKLLCETSL
DSIEDILTRNKPEIVIIDSIQTMYKEDVAAAPGSVSQVRETTSILMQLAKGLNITIFIVGHVTKEGTVAGPRMLEHMVDT
VLYFEGDRYASYRILRGVKNRFGSTNEIGVFEMQQSGLSEVENPSEYMLNGRPENASGSVVVCLLEGTRPIMVEIQALVC
DSNFGMARRTAAGTDYNRVNLLMAVLEKRAGIHMSGSDAYVNVAGGIKVSEPALDLGIVMALVSSFKNKAIDEKTVIFGE
VGLAGEVRAVSQAQQRVNEAVKLGFKTCILPGVCLKNIKKNDKIEIIGVNNVQEAIKLI

Sequences:

>Translated_459_residues
MAKAKNTAFFCKECGYESAKWFGQCPACKEWNTFVEEPVSKPKSAKGIAGTSQRAAASSAPVPVEINSVSFEENDRTSTG
IAELDRVLGGGIVAGSLVLVGGDPGIGKSTLLLQMCYNLSAEGKDVLYISGEESLKQIKLRADRIGKCSGTLKLLCETSL
DSIEDILTRNKPEIVIIDSIQTMYKEDVAAAPGSVSQVRETTSILMQLAKGLNITIFIVGHVTKEGTVAGPRMLEHMVDT
VLYFEGDRYASYRILRGVKNRFGSTNEIGVFEMQQSGLSEVENPSEYMLNGRPENASGSVVVCLLEGTRPIMVEIQALVC
DSNFGMARRTAAGTDYNRVNLLMAVLEKRAGIHMSGSDAYVNVAGGIKVSEPALDLGIVMALVSSFKNKAIDEKTVIFGE
VGLAGEVRAVSQAQQRVNEAVKLGFKTCILPGVCLKNIKKNDKIEIIGVNNVQEAIKLI
>Mature_458_residues
AKAKNTAFFCKECGYESAKWFGQCPACKEWNTFVEEPVSKPKSAKGIAGTSQRAAASSAPVPVEINSVSFEENDRTSTGI
AELDRVLGGGIVAGSLVLVGGDPGIGKSTLLLQMCYNLSAEGKDVLYISGEESLKQIKLRADRIGKCSGTLKLLCETSLD
SIEDILTRNKPEIVIIDSIQTMYKEDVAAAPGSVSQVRETTSILMQLAKGLNITIFIVGHVTKEGTVAGPRMLEHMVDTV
LYFEGDRYASYRILRGVKNRFGSTNEIGVFEMQQSGLSEVENPSEYMLNGRPENASGSVVVCLLEGTRPIMVEIQALVCD
SNFGMARRTAAGTDYNRVNLLMAVLEKRAGIHMSGSDAYVNVAGGIKVSEPALDLGIVMALVSSFKNKAIDEKTVIFGEV
GLAGEVRAVSQAQQRVNEAVKLGFKTCILPGVCLKNIKKNDKIEIIGVNNVQEAIKLI

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=465, Percent_Identity=44.9462365591398, Blast_Score=413, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49390; Mature: 49258

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50162 RECA_2 ; PS00127 RNASE_PANCREATIC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKNTAFFCKECGYESAKWFGQCPACKEWNTFVEEPVSKPKSAKGIAGTSQRAAASSA
CCCCCCCCHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCC
PVPVEINSVSFEENDRTSTGIAELDRVLGGGIVAGSLVLVGGDPGIGKSTLLLQMCYNLS
CCEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEECEEEEECCCCCCCHHHHHHHHHHCCC
AEGKDVLYISGEESLKQIKLRADRIGKCSGTLKLLCETSLDSIEDILTRNKPEIVIIDSI
CCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCH
QTMYKEDVAAAPGSVSQVRETTSILMQLAKGLNITIFIVGHVTKEGTVAGPRMLEHMVDT
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHH
VLYFEGDRYASYRILRGVKNRFGSTNEIGVFEMQQSGLSEVENPSEYMLNGRPENASGSV
HEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECHHCHHHCCCCHHHHCCCCCCCCCCCE
VVCLLEGTRPIMVEIQALVCDSNFGMARRTAAGTDYNRVNLLMAVLEKRAGIHMSGSDAY
EEEEECCCCCEEEEEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCEECCCCCE
VNVAGGIKVSEPALDLGIVMALVSSFKNKAIDEKTVIFGEVGLAGEVRAVSQAQQRVNEA
EEEECCCEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
VKLGFKTCILPGVCLKNIKKNDKIEIIGVNNVQEAIKLI
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCC
>Mature Secondary Structure 
AKAKNTAFFCKECGYESAKWFGQCPACKEWNTFVEEPVSKPKSAKGIAGTSQRAAASSA
CCCCCCCHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCC
PVPVEINSVSFEENDRTSTGIAELDRVLGGGIVAGSLVLVGGDPGIGKSTLLLQMCYNLS
CCEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEECEEEEECCCCCCCHHHHHHHHHHCCC
AEGKDVLYISGEESLKQIKLRADRIGKCSGTLKLLCETSLDSIEDILTRNKPEIVIIDSI
CCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCH
QTMYKEDVAAAPGSVSQVRETTSILMQLAKGLNITIFIVGHVTKEGTVAGPRMLEHMVDT
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHH
VLYFEGDRYASYRILRGVKNRFGSTNEIGVFEMQQSGLSEVENPSEYMLNGRPENASGSV
HEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECHHCHHHCCCCHHHHCCCCCCCCCCCE
VVCLLEGTRPIMVEIQALVCDSNFGMARRTAAGTDYNRVNLLMAVLEKRAGIHMSGSDAY
EEEEECCCCCEEEEEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCEECCCCCE
VNVAGGIKVSEPALDLGIVMALVSSFKNKAIDEKTVIFGEVGLAGEVRAVSQAQQRVNEA
EEEECCCEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
VKLGFKTCILPGVCLKNIKKNDKIEIIGVNNVQEAIKLI
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]