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The map label for this gene is sigA [H]

Identifier: 238916620

GI number: 238916620

Start: 676594

End: 677898

Strand: Direct

Name: sigA [H]

Synonym: EUBELI_00677

Alternate gene names: 238916620

Gene position: 676594-677898 (Clockwise)

Preceding gene: 238916619

Following gene: 238916621

Centisome position: 31.55

GC content: 38.47

Gene sequence:

>1305_bases
GTGTTACGCGAAGAAGAATGGAGAAGAGAAATGGCTAAGAAAATGGTAGAAACATTAGAAGAGAATGTTAAGGAGACTAA
GGAAGAAAAGACAACAGAAAAGAAGACTCCTGCAAAAAAAGCAGCAGCAAAGAAGACAACTAAGAAAGATGAGATTAAAG
AAGAAGTAAAAGAAGAGACAGAAAACAGTGACGCAGATGATGCAGAAGCACAGGAAGCTAAATTCTGGGAGAAGATAGAG
GGTATTCTTGCAATAGCCAAGAAGAAAAAGAATGTACTTGATTATCAGGAAATTATGACATATTTTCAGGATACAGATTT
TGAAGCTGACAGAATGGAGAAGGTATTTGAAATTCTTGAGCTAAAAAAAGTAGATGTAAGAATGAATGATGTACCTGATG
AAGATGAGGATATTATTCTTGATGATGAAGATGAGATTGATATTGAGAAGATAGATCTTTCTGTTCCTGATGGAATCGGT
CTTGATGATCCAGTAAGAATGTATCTGAAGGAGATAGGTAAAGTACCTCTTCTTAGTGCTGATGAAGAGATTGAATATGC
CAAGAGAATGGAAGAAGGAGATGAGGAAGCCAAGAAAAGACTTGCAGAAGCTAACTTAAGACTTGTTGTAAGTATTGCAA
AACGTTATGTTGGACGTGGTATGCAGTTCCTTGATCTTATTCAGGAAGGTAACTTAGGTCTTATAAAGGCTGTTGAGAAA
TACGATTACAGAAAAGGATTCAAGTTCAGTACATACGCTACATGGTGGATCAGACAGGCTATTACAAGAGCTATAGCTGA
CCAGGCCCGTACTATAAGAATTCCTGTTCATATGGTAGAGACAATTAACAAGCTTGTAAGAGTACAGAGACAGCTGCTTC
AGGAACTTGGAAGAGAACCATCACCAGAGGAGATTGCAGAGAATATGGATATTCCTGTAGAGAGAGTAAGAGAGATTCAG
AAGATTTCGCAGGAACCGGTATCTCTTGAGACACCTATTGGTGAAGAGGAAGATAGCCATCTTGGAGATTTCATTCAGGA
TGATAATGTTCCTGTTCCGGCAGAGGCAGCAGCTTCTACACTTCTTAAGGAACAGCTTGTTGAAGTACTTGGAACTCTTA
CAGAAAGAGAGCAGAAGGTATTAAGATTAAGATTTGGTATGGATGATGGAAGAGCAAGAACTCTTGAGGAAGTTGGTAAA
GAATTCAATGTAACAAGAGAACGTATCAGACAGATTGAAGCCAAGGCATTAAGAAAGTTAAGACACCCAAGCCGCAGCCG
TAAGTTAAGAGATTATCTTGATTAG

Upstream 100 bases:

>100_bases
ATGAACTTAATAAGACTGTAGATCCGAAGCGTATGCAGGAGATAATAATTGAACAGCAGAAGCTGAATCAGATTCATATT
AAGTTATAAATATATAGAGC

Downstream 100 bases:

>100_bases
GATTAAGCTATGGTAAGAATATCGGACAGATTAAGAACAGTTGCACATATGTGTAATAAGGGCGCAGTCGTGGCTGACAT
AGGTACAGACCACGGCTATC

Product: RNA polymerase primary sigma factor

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MLREEEWRREMAKKMVETLEENVKETKEEKTTEKKTPAKKAAAKKTTKKDEIKEEVKEETENSDADDAEAQEAKFWEKIE
GILAIAKKKKNVLDYQEIMTYFQDTDFEADRMEKVFEILELKKVDVRMNDVPDEDEDIILDDEDEIDIEKIDLSVPDGIG
LDDPVRMYLKEIGKVPLLSADEEIEYAKRMEEGDEEAKKRLAEANLRLVVSIAKRYVGRGMQFLDLIQEGNLGLIKAVEK
YDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLVRVQRQLLQELGREPSPEEIAENMDIPVERVREIQ
KISQEPVSLETPIGEEEDSHLGDFIQDDNVPVPAEAAASTLLKEQLVEVLGTLTEREQKVLRLRFGMDDGRARTLEEVGK
EFNVTRERIRQIEAKALRKLRHPSRSRKLRDYLD

Sequences:

>Translated_434_residues
MLREEEWRREMAKKMVETLEENVKETKEEKTTEKKTPAKKAAAKKTTKKDEIKEEVKEETENSDADDAEAQEAKFWEKIE
GILAIAKKKKNVLDYQEIMTYFQDTDFEADRMEKVFEILELKKVDVRMNDVPDEDEDIILDDEDEIDIEKIDLSVPDGIG
LDDPVRMYLKEIGKVPLLSADEEIEYAKRMEEGDEEAKKRLAEANLRLVVSIAKRYVGRGMQFLDLIQEGNLGLIKAVEK
YDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLVRVQRQLLQELGREPSPEEIAENMDIPVERVREIQ
KISQEPVSLETPIGEEEDSHLGDFIQDDNVPVPAEAAASTLLKEQLVEVLGTLTEREQKVLRLRFGMDDGRARTLEEVGK
EFNVTRERIRQIEAKALRKLRHPSRSRKLRDYLD
>Mature_434_residues
MLREEEWRREMAKKMVETLEENVKETKEEKTTEKKTPAKKAAAKKTTKKDEIKEEVKEETENSDADDAEAQEAKFWEKIE
GILAIAKKKKNVLDYQEIMTYFQDTDFEADRMEKVFEILELKKVDVRMNDVPDEDEDIILDDEDEIDIEKIDLSVPDGIG
LDDPVRMYLKEIGKVPLLSADEEIEYAKRMEEGDEEAKKRLAEANLRLVVSIAKRYVGRGMQFLDLIQEGNLGLIKAVEK
YDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLVRVQRQLLQELGREPSPEEIAENMDIPVERVREIQ
KISQEPVSLETPIGEEEDSHLGDFIQDDNVPVPAEAAASTLLKEQLVEVLGTLTEREQKVLRLRFGMDDGRARTLEEVGK
EFNVTRERIRQIEAKALRKLRHPSRSRKLRDYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=250, Percent_Identity=66, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI1789098, Length=267, Percent_Identity=46.4419475655431, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=30.8823529411765, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788231, Length=199, Percent_Identity=29.1457286432161, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 50465; Mature: 50465

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLREEEWRREMAKKMVETLEENVKETKEEKTTEKKTPAKKAAAKKTTKKDEIKEEVKEET
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHH
ENSDADDAEAQEAKFWEKIEGILAIAKKKKNVLDYQEIMTYFQDTDFEADRMEKVFEILE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LKKVDVRMNDVPDEDEDIILDDEDEIDIEKIDLSVPDGIGLDDPVRMYLKEIGKVPLLSA
HHHHCCEECCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCC
DEEIEYAKRMEEGDEEAKKRLAEANLRLVVSIAKRYVGRGMQFLDLIQEGNLGLIKAVEK
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHH
YDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLVRVQRQLLQELGREP
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPEEIAENMDIPVERVREIQKISQEPVSLETPIGEEEDSHLGDFIQDDNVPVPAEAAAST
CHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHH
LLKEQLVEVLGTLTEREQKVLRLRFGMDDGRARTLEEVGKEFNVTRERIRQIEAKALRKL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHPSRSRKLRDYLD
HCCHHHHHHHHHCC
>Mature Secondary Structure
MLREEEWRREMAKKMVETLEENVKETKEEKTTEKKTPAKKAAAKKTTKKDEIKEEVKEET
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHH
ENSDADDAEAQEAKFWEKIEGILAIAKKKKNVLDYQEIMTYFQDTDFEADRMEKVFEILE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LKKVDVRMNDVPDEDEDIILDDEDEIDIEKIDLSVPDGIGLDDPVRMYLKEIGKVPLLSA
HHHHCCEECCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCC
DEEIEYAKRMEEGDEEAKKRLAEANLRLVVSIAKRYVGRGMQFLDLIQEGNLGLIKAVEK
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHH
YDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLVRVQRQLLQELGREP
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPEEIAENMDIPVERVREIQKISQEPVSLETPIGEEEDSHLGDFIQDDNVPVPAEAAAST
CHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHH
LLKEQLVEVLGTLTEREQKVLRLRFGMDDGRARTLEEVGKEFNVTRERIRQIEAKALRKL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHPSRSRKLRDYLD
HCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]