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The map label for this gene is dnaG [H]

Identifier: 238916619

GI number: 238916619

Start: 674819

End: 676582

Strand: Direct

Name: dnaG [H]

Synonym: EUBELI_00676

Alternate gene names: 238916619

Gene position: 674819-676582 (Clockwise)

Preceding gene: 238916618

Following gene: 238916620

Centisome position: 31.47

GC content: 40.53

Gene sequence:

>1764_bases
ATGTATTCGAGAGAAGTAATAGATGAAGTAATATCCCGGAATGATATTGTTGATGTTATATCCGGGTATGTCAAATTGAA
AAAGAATGGAAGTTCATATACCGGACTATGTCCGTTTCATAATGAGAAATCACCATCATTTTCGGTTTCAGGACAGCGGC
AGTTATATCATTGTTTTGGATGCGGCGCTGGGGGTAATGTCATAACATTTGTAATGGAATATGAGAATATGACATTCTTA
GAGGCAGTTAAGATGCTGGGAGAGAGAGCCGGAGTTGCACTGCCGCAGACGTCTATGTCAGAAGAAGACCGCAAAGAAAG
AGGGATAAGAGACAGGCTTTTAGAGATTAATAAGATTGCTGCCACATATTATTACAGGCAGCTTAGAAGTGAGAATGGGA
AAGCCGGACTTGATTATCTTAAGAAGCGTGAATTGTCTGACAGTACGATTAACAGCTTTGGACTGGGGTATGCAACACAG
TCAACAGGTAATCTGTACAAGCTGTTGAAGGACAAAGGTTATGATGATGATATTCTGAAGGAATCAGGACTTTTTACTTA
TGAGAGAGGCATTCATGAAAAGTTCTGGAACAGAGTAATTTTTCCAATTATGGATATTAATAATAAGGTCATAGGATTTG
GTGGACGAGTTATGGGTGATGCCAAGCCAAAGTACCTTAATTCACCGGAGACAAGATTGTTTGACAAGAGCCGCAATCTG
TATGGACTTAATATAGCAAGAACGTCAAGAAAGCCTAACATGATAATATGTGAAGGCTATATGGATGTTATATCAATGCA
TCAGGCGGGCTTTAACCAGGCGGTTGCGTCACTTGGAACGGCACTGACACCGGGACATGCAAGGCTGTTAAAGAGATATA
CAGATAATGTGCTTATAACTTATGACAGTGATGAGGCAGGAGTTAAGGCGGCACTTCGAGCAATACCGATTCTTAAGGAA
GCAGGGCTTTCAACGAAGGTAATTAATATGCGGCCATATAAGGATCCTGATGAGTTTATTAAGGCTATGGGTGCAGAGGC
ATTTCAGGAAAGAATTGATAATGCAGAGAACAGCTTTATGTATGAGATAGGAACCATGTTAAGAAATTACGACAGGGGGG
ATCCGGAAAGCGAGACTGCATTTGAAAGAGAGGTTGCAGCAAAGCTTGTTACATTTAAAGAAAAGCTGGAACGTGATAAT
TACCTTAAAGCAGTGTGCAGGCAGTTTATGATACCAGAAGATGGCATGAGGCAGATGGTATCAAGACTTGGTAATCAGGA
AGGGATTATTTCAAGAAATGCAGGTGCAGGTACTCCGCAGCCAACTGTTGAGCGTAAGCCGAAGAAGAAGCGTGAAGATG
GTTTAAGACAGGCAGAGAAAATGCTTCTTACATGGCTCATAAGTGACAGGGACATATTTGATAAGGTTGCAGGTTACATT
AAGCCAGAGGATTTTATTGACCCGTTATTTTCGGGTGTGGCAGAGAAAGTATATGAACAGTATGGGACAGGAAATATAAG
TCCTGCTGCAATTATAGCTGATTATGCAACAACAGAAGAACATACGGAAGTCGCAGCAATGTTTTCGGCAGACCTCGATG
AGAGCCTTAATAATCTGGAAAGAGAAAAGACTCTTAATGACATTGTTATCAGAATTAAGGCTAACAGCCTTAATCATGAA
CTTAATAAGACTGTAGATCCGAAGCGTATGCAGGAGATAATAATTGAACAGCAGAAGCTGAATCAGATTCATATTAAGTT
ATAA

Upstream 100 bases:

>100_bases
GAGCAGGTTGTGTGTGATTATATAGCTGGCATGAGTGACCAGTATTCAATAAGTAAGTTTCAGGAAATCTTTGTACCAAA
GGCCTGGAAGGGATGAAGTA

Downstream 100 bases:

>100_bases
ATATATAGAGCGTGTTACGCGAAGAAGAATGGAGAAGAGAAATGGCTAAGAAAATGGTAGAAACATTAGAAGAGAATGTT
AAGGAGACTAAGGAAGAAAA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MYSREVIDEVISRNDIVDVISGYVKLKKNGSSYTGLCPFHNEKSPSFSVSGQRQLYHCFGCGAGGNVITFVMEYENMTFL
EAVKMLGERAGVALPQTSMSEEDRKERGIRDRLLEINKIAATYYYRQLRSENGKAGLDYLKKRELSDSTINSFGLGYATQ
STGNLYKLLKDKGYDDDILKESGLFTYERGIHEKFWNRVIFPIMDINNKVIGFGGRVMGDAKPKYLNSPETRLFDKSRNL
YGLNIARTSRKPNMIICEGYMDVISMHQAGFNQAVASLGTALTPGHARLLKRYTDNVLITYDSDEAGVKAALRAIPILKE
AGLSTKVINMRPYKDPDEFIKAMGAEAFQERIDNAENSFMYEIGTMLRNYDRGDPESETAFEREVAAKLVTFKEKLERDN
YLKAVCRQFMIPEDGMRQMVSRLGNQEGIISRNAGAGTPQPTVERKPKKKREDGLRQAEKMLLTWLISDRDIFDKVAGYI
KPEDFIDPLFSGVAEKVYEQYGTGNISPAAIIADYATTEEHTEVAAMFSADLDESLNNLEREKTLNDIVIRIKANSLNHE
LNKTVDPKRMQEIIIEQQKLNQIHIKL

Sequences:

>Translated_587_residues
MYSREVIDEVISRNDIVDVISGYVKLKKNGSSYTGLCPFHNEKSPSFSVSGQRQLYHCFGCGAGGNVITFVMEYENMTFL
EAVKMLGERAGVALPQTSMSEEDRKERGIRDRLLEINKIAATYYYRQLRSENGKAGLDYLKKRELSDSTINSFGLGYATQ
STGNLYKLLKDKGYDDDILKESGLFTYERGIHEKFWNRVIFPIMDINNKVIGFGGRVMGDAKPKYLNSPETRLFDKSRNL
YGLNIARTSRKPNMIICEGYMDVISMHQAGFNQAVASLGTALTPGHARLLKRYTDNVLITYDSDEAGVKAALRAIPILKE
AGLSTKVINMRPYKDPDEFIKAMGAEAFQERIDNAENSFMYEIGTMLRNYDRGDPESETAFEREVAAKLVTFKEKLERDN
YLKAVCRQFMIPEDGMRQMVSRLGNQEGIISRNAGAGTPQPTVERKPKKKREDGLRQAEKMLLTWLISDRDIFDKVAGYI
KPEDFIDPLFSGVAEKVYEQYGTGNISPAAIIADYATTEEHTEVAAMFSADLDESLNNLEREKTLNDIVIRIKANSLNHE
LNKTVDPKRMQEIIIEQQKLNQIHIKL
>Mature_587_residues
MYSREVIDEVISRNDIVDVISGYVKLKKNGSSYTGLCPFHNEKSPSFSVSGQRQLYHCFGCGAGGNVITFVMEYENMTFL
EAVKMLGERAGVALPQTSMSEEDRKERGIRDRLLEINKIAATYYYRQLRSENGKAGLDYLKKRELSDSTINSFGLGYATQ
STGNLYKLLKDKGYDDDILKESGLFTYERGIHEKFWNRVIFPIMDINNKVIGFGGRVMGDAKPKYLNSPETRLFDKSRNL
YGLNIARTSRKPNMIICEGYMDVISMHQAGFNQAVASLGTALTPGHARLLKRYTDNVLITYDSDEAGVKAALRAIPILKE
AGLSTKVINMRPYKDPDEFIKAMGAEAFQERIDNAENSFMYEIGTMLRNYDRGDPESETAFEREVAAKLVTFKEKLERDN
YLKAVCRQFMIPEDGMRQMVSRLGNQEGIISRNAGAGTPQPTVERKPKKKREDGLRQAEKMLLTWLISDRDIFDKVAGYI
KPEDFIDPLFSGVAEKVYEQYGTGNISPAAIIADYATTEEHTEVAAMFSADLDESLNNLEREKTLNDIVIRIKANSLNHE
LNKTVDPKRMQEIIIEQQKLNQIHIKL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=381, Percent_Identity=39.8950131233596, Blast_Score=279, Evalue=3e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 66483; Mature: 66483

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSREVIDEVISRNDIVDVISGYVKLKKNGSSYTGLCPFHNEKSPSFSVSGQRQLYHCFG
CCCHHHHHHHHCCCHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEC
CGAGGNVITFVMEYENMTFLEAVKMLGERAGVALPQTSMSEEDRKERGIRDRLLEINKIA
CCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHH
ATYYYRQLRSENGKAGLDYLKKRELSDSTINSFGLGYATQSTGNLYKLLKDKGYDDDILK
HHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHH
ESGLFTYERGIHEKFWNRVIFPIMDINNKVIGFGGRVMGDAKPKYLNSPETRLFDKSRNL
HCCCCHHHHHHHHHHHHHHHHHEECCCCCEEECCCEECCCCCCCCCCCCHHHHHHCCCCE
YGLNIARTSRKPNMIICEGYMDVISMHQAGFNQAVASLGTALTPGHARLLKRYTDNVLIT
EEEEEEECCCCCCEEEECCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEE
YDSDEAGVKAALRAIPILKEAGLSTKVINMRPYKDPDEFIKAMGAEAFQERIDNAENSFM
ECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCHHHHHHHHCCCHHHHH
YEIGTMLRNYDRGDPESETAFEREVAAKLVTFKEKLERDNYLKAVCRQFMIPEDGMRQMV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
SRLGNQEGIISRNAGAGTPQPTVERKPKKKREDGLRQAEKMLLTWLISDRDIFDKVAGYI
HHCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
KPEDFIDPLFSGVAEKVYEQYGTGNISPAAIIADYATTEEHTEVAAMFSADLDESLNNLE
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEHHHCCHHHHHHHHHHHHHHHHHHHHHH
REKTLNDIVIRIKANSLNHELNKTVDPKRMQEIIIEQQKLNQIHIKL
HHHHHHHEEEEEECCCCCHHHHCCCCHHHHHHHHHHHHHCCEEEEEC
>Mature Secondary Structure
MYSREVIDEVISRNDIVDVISGYVKLKKNGSSYTGLCPFHNEKSPSFSVSGQRQLYHCFG
CCCHHHHHHHHCCCHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEC
CGAGGNVITFVMEYENMTFLEAVKMLGERAGVALPQTSMSEEDRKERGIRDRLLEINKIA
CCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHH
ATYYYRQLRSENGKAGLDYLKKRELSDSTINSFGLGYATQSTGNLYKLLKDKGYDDDILK
HHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHH
ESGLFTYERGIHEKFWNRVIFPIMDINNKVIGFGGRVMGDAKPKYLNSPETRLFDKSRNL
HCCCCHHHHHHHHHHHHHHHHHEECCCCCEEECCCEECCCCCCCCCCCCHHHHHHCCCCE
YGLNIARTSRKPNMIICEGYMDVISMHQAGFNQAVASLGTALTPGHARLLKRYTDNVLIT
EEEEEEECCCCCCEEEECCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEE
YDSDEAGVKAALRAIPILKEAGLSTKVINMRPYKDPDEFIKAMGAEAFQERIDNAENSFM
ECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCHHHHHHHHCCCHHHHH
YEIGTMLRNYDRGDPESETAFEREVAAKLVTFKEKLERDNYLKAVCRQFMIPEDGMRQMV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
SRLGNQEGIISRNAGAGTPQPTVERKPKKKREDGLRQAEKMLLTWLISDRDIFDKVAGYI
HHCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
KPEDFIDPLFSGVAEKVYEQYGTGNISPAAIIADYATTEEHTEVAAMFSADLDESLNNLE
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEHHHCCHHHHHHHHHHHHHHHHHHHHHH
REKTLNDIVIRIKANSLNHELNKTVDPKRMQEIIIEQQKLNQIHIKL
HHHHHHHEEEEEECCCCCHHHHCCCCHHHHHHHHHHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]