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The map label for this gene is hisS [H]

Identifier: 238916495

GI number: 238916495

Start: 556837

End: 558111

Strand: Direct

Name: hisS [H]

Synonym: EUBELI_00552

Alternate gene names: 238916495

Gene position: 556837-558111 (Clockwise)

Preceding gene: 238916494

Following gene: 238916497

Centisome position: 25.97

GC content: 39.06

Gene sequence:

>1275_bases
ATGAAAATAACACCAGTTAAGGGTACTAATGATTATCTTCCTAATGAGGTTGAAATCAGAGATTACCTTCAGAATGAGAT
ATTAAAGGTATATGTTGCTAATGGATTTGAACACATTACAACACCTATTATTGAGGATATTGAGAATCTTGATAAGAGTG
ATGGTGGTGAGAACTTAAATCTTATATTCAAGATTATGAAGAGAGGCGATAAGCTTGAGAAGGCTGTAAGTTCACTTCAG
GAAAATCCTAAGACAGGAACAGCCTGTGAGAATGAAATTGCTGATATGGGATTAAGATATGACCTTACACTTCCACTCAG
CCGTTATTTTGCTAACAACAAGGACAAGCTCACTCTTCCTATGAAATGTATCCAGATGGACAGAGTGTACAGAGCTGAAA
GACCACAGAAGGGCAGACTTCGTGAGTTTGTTCAGTGTGATATTGATATTATTGGTTCTGATTCTACAGATTCAGAGGTT
GAACTTATTCTTACAACAACCAAGGCCCTTAAGGCTATTGGCATGAAGAATTTCAAGGTTAAGATTAACGACAGAAGACT
TCTACGTTCATTCCTTCAGAATTGTGGATTTGCAGAGGAGCAGCTTGACAGTGTGTGCATTACATTTGACAAGATGGATA
AGGTCGGACTTGATGGTGTTAAGGCTGAGCTTACAGATAAGGGCTTTGATGCTGCTGCAATTGAGAAGTTTATCGGATTC
TTTGGAAGTGTTTCAAGTGCACAGGAGATAAGCCTTGAAAGTGTTGAGGCAATTCTTGAAGATAAAGCTCCGGCAGAGAG
TGTAAGAGGAATCATTAATACAGTAAATGAGCTTTCAGACGGTCAGTTTGATGTTGTATTTGACCTTTCTTTAGTAAGAG
GTCAGGGTTACTATACAGGAACTGTATTTGAAGTTGAAAGCATTGATTTCAAGGGTGCTATCGCAGGCGGTGGAAGATAT
GATAATCTTATAGGAAAGTTCATAGGACAGCAGGTAAGCGCTGTTGGATTCTCAATCGGATTCGAGAGAATTTTCTCAAT
CCTTATGCAGAATGGCGTTGAGACTAAGGCATCAGCAGACAAGATTGCTGTTATGTACGATGAGGGACAGGTTAAGGAAG
CTTATGCAATAGCAGAAAAATACAGAGCAGAAGGTAAAGTATGCTCATTATATGTAAAGCCTAAGAAGATGGGAAAGTTC
CTCGGAAAGCTTGAGGAACGTGGATATAAAGGATTTGTTAATGTCAGCAATGGTGATGAGATAAGCTTGTTCTAG

Upstream 100 bases:

>100_bases
TTTTCGATACTGGCGGAAAATAGTTAGTATTAAAGAAAATGAGGGCAGATGGCTGGAGAATACCGGTTATCTGCCTTAGT
GTGTAAATGAGGAGACAACT

Downstream 100 bases:

>100_bases
TGAGTTTTATGGCGCAGCCTTTTTAAGGGCTGCGCTTATTGCTTCTATAAAGACGGAATATGTATATCTGTTGGCTGAAT
CATAGGTTATGTTGGTTGTG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MKITPVKGTNDYLPNEVEIRDYLQNEILKVYVANGFEHITTPIIEDIENLDKSDGGENLNLIFKIMKRGDKLEKAVSSLQ
ENPKTGTACENEIADMGLRYDLTLPLSRYFANNKDKLTLPMKCIQMDRVYRAERPQKGRLREFVQCDIDIIGSDSTDSEV
ELILTTTKALKAIGMKNFKVKINDRRLLRSFLQNCGFAEEQLDSVCITFDKMDKVGLDGVKAELTDKGFDAAAIEKFIGF
FGSVSSAQEISLESVEAILEDKAPAESVRGIINTVNELSDGQFDVVFDLSLVRGQGYYTGTVFEVESIDFKGAIAGGGRY
DNLIGKFIGQQVSAVGFSIGFERIFSILMQNGVETKASADKIAVMYDEGQVKEAYAIAEKYRAEGKVCSLYVKPKKMGKF
LGKLEERGYKGFVNVSNGDEISLF

Sequences:

>Translated_424_residues
MKITPVKGTNDYLPNEVEIRDYLQNEILKVYVANGFEHITTPIIEDIENLDKSDGGENLNLIFKIMKRGDKLEKAVSSLQ
ENPKTGTACENEIADMGLRYDLTLPLSRYFANNKDKLTLPMKCIQMDRVYRAERPQKGRLREFVQCDIDIIGSDSTDSEV
ELILTTTKALKAIGMKNFKVKINDRRLLRSFLQNCGFAEEQLDSVCITFDKMDKVGLDGVKAELTDKGFDAAAIEKFIGF
FGSVSSAQEISLESVEAILEDKAPAESVRGIINTVNELSDGQFDVVFDLSLVRGQGYYTGTVFEVESIDFKGAIAGGGRY
DNLIGKFIGQQVSAVGFSIGFERIFSILMQNGVETKASADKIAVMYDEGQVKEAYAIAEKYRAEGKVCSLYVKPKKMGKF
LGKLEERGYKGFVNVSNGDEISLF
>Mature_424_residues
MKITPVKGTNDYLPNEVEIRDYLQNEILKVYVANGFEHITTPIIEDIENLDKSDGGENLNLIFKIMKRGDKLEKAVSSLQ
ENPKTGTACENEIADMGLRYDLTLPLSRYFANNKDKLTLPMKCIQMDRVYRAERPQKGRLREFVQCDIDIIGSDSTDSEV
ELILTTTKALKAIGMKNFKVKINDRRLLRSFLQNCGFAEEQLDSVCITFDKMDKVGLDGVKAELTDKGFDAAAIEKFIGF
FGSVSSAQEISLESVEAILEDKAPAESVRGIINTVNELSDGQFDVVFDLSLVRGQGYYTGTVFEVESIDFKGAIAGGGRY
DNLIGKFIGQQVSAVGFSIGFERIFSILMQNGVETKASADKIAVMYDEGQVKEAYAIAEKYRAEGKVCSLYVKPKKMGKF
LGKLEERGYKGFVNVSNGDEISLF

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=434, Percent_Identity=32.7188940092166, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI15029520, Length=375, Percent_Identity=30.9333333333333, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI65287717, Length=319, Percent_Identity=23.5109717868339, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788861, Length=407, Percent_Identity=28.009828009828, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71993686, Length=378, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71993693, Length=378, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325290, Length=408, Percent_Identity=29.9019607843137, Blast_Score=149, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24643059, Length=427, Percent_Identity=32.7868852459016, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24643061, Length=424, Percent_Identity=32.5471698113208, Blast_Score=171, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47169; Mature: 47169

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITPVKGTNDYLPNEVEIRDYLQNEILKVYVANGFEHITTPIIEDIENLDKSDGGENLN
CEECCCCCCCCCCCCCHHHHHHHHHHHEEHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHH
LIFKIMKRGDKLEKAVSSLQENPKTGTACENEIADMGLRYDLTLPLSRYFANNKDKLTLP
HHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECCHHHHHCCCCCEEEHH
MKCIQMDRVYRAERPQKGRLREFVQCDIDIIGSDSTDSEVELILTTTKALKAIGMKNFKV
HHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCCCCEEEEEEEHHHHHHHCCCCEEE
KINDRRLLRSFLQNCGFAEEQLDSVCITFDKMDKVGLDGVKAELTDKGFDAAAIEKFIGF
EECHHHHHHHHHHHCCCCHHHHHHEEEEHHHHHHCCCCCCEEHHCCCCCCHHHHHHHHHH
FGSVSSAQEISLESVEAILEDKAPAESVRGIINTVNELSDGQFDVVFDLSLVRGQGYYTG
HCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCEEEE
TVFEVESIDFKGAIAGGGRYDNLIGKFIGQQVSAVGFSIGFERIFSILMQNGVETKASAD
EEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KIAVMYDEGQVKEAYAIAEKYRAEGKVCSLYVKPKKMGKFLGKLEERGYKGFVNVSNGDE
EEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCEEEECCCCCE
ISLF
EEEC
>Mature Secondary Structure
MKITPVKGTNDYLPNEVEIRDYLQNEILKVYVANGFEHITTPIIEDIENLDKSDGGENLN
CEECCCCCCCCCCCCCHHHHHHHHHHHEEHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHH
LIFKIMKRGDKLEKAVSSLQENPKTGTACENEIADMGLRYDLTLPLSRYFANNKDKLTLP
HHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECCHHHHHCCCCCEEEHH
MKCIQMDRVYRAERPQKGRLREFVQCDIDIIGSDSTDSEVELILTTTKALKAIGMKNFKV
HHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCCCCEEEEEEEHHHHHHHCCCCEEE
KINDRRLLRSFLQNCGFAEEQLDSVCITFDKMDKVGLDGVKAELTDKGFDAAAIEKFIGF
EECHHHHHHHHHHHCCCCHHHHHHEEEEHHHHHHCCCCCCEEHHCCCCCCHHHHHHHHHH
FGSVSSAQEISLESVEAILEDKAPAESVRGIINTVNELSDGQFDVVFDLSLVRGQGYYTG
HCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCEEEE
TVFEVESIDFKGAIAGGGRYDNLIGKFIGQQVSAVGFSIGFERIFSILMQNGVETKASAD
EEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KIAVMYDEGQVKEAYAIAEKYRAEGKVCSLYVKPKKMGKFLGKLEERGYKGFVNVSNGDE
EEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCEEEECCCCCE
ISLF
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA