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The map label for this gene is cysS

Identifier: 238916434

GI number: 238916434

Start: 497960

End: 499378

Strand: Direct

Name: cysS

Synonym: EUBELI_00490

Alternate gene names: 238916434

Gene position: 497960-499378 (Clockwise)

Preceding gene: 238916433

Following gene: 238916435

Centisome position: 23.22

GC content: 37.49

Gene sequence:

>1419_bases
ATGAGGATTTATAATACTTTATCCAGAAGAAAAGAAGAGTTTAAGCCATTAGAAGAAGGTAAGGTTAAAATGTATGTCTG
CGGACCTACTGTATACAACCTTATTCATATTGGTAACGCAAGACCTATGATTGTATTTGATACAGTAAGAAGATATCTTG
AGTACAAGGGATATGATGTTAATTATGTATCTAACTTTACAGATGTAGACGACAAGATTATTAAGAAAGCTAATGAAGAA
GGTGTATCTGCTGAGGAGATTTCAACAAGATACATTGCTGAATGTAAGAAGGATATGGAGGGCATGAATATTAAGCCTGC
AACAACACATCCTCTTGCAACTCAGGAAATCGGTGGAATGATTGATATGATACAGACTCTTATTGACAAGGGTTATGCTT
ATGAAGTCAATGGAACCGTATACTACAGAACAAGAAAGTTTGCAGAATACGGAAAGCTATCTAAGAAGAATATTGACGAC
TTGGAGGCAGGACACAGAGATGAGGCACATAGTCTTAAGGTTACAGGTGAGGATGAGAAGGAAGACCCACTTGATTTCGT
TCTTTGGAAGCCTAAGAAAGAAGGAGAGCCATCTTGGCCATCACCTTGGAGTGACGGAAGACCAGGCTGGCATATTGAAT
GCTCTGTTATGTCTAAGAAGTATCTTGCTGACGAAATCGATATTCATGCAGGTGGAGAGGATTTAATATTCCCTCACCAT
GAGAATGAGATTGCACAGTCAGAGGCAGCTAATGGAGTACCATTCTCTAAGTACTGGATGCACAATGCATTTCTTAATAT
TGATAACAAGAAGATGTCTAAGTCTTTAGGCAATTTCTTTACAGTAAGAGATATAAGCAGGGAATATGACCTTGAAGTGT
TAAGATTCTTCATGTTAAGCGCACATTACCGCAATCCTGTTAATTTCAGTCATGACCTTATGGAATCAGCAAAGAACGGA
TTAGACAGAATCTTAACTGCAGTTGATAATTTAAGACATCTGTCAGAGAATGCCAAAGATGTTATAATGACAGAGGATGA
GAAGAAGATAATAGCTTCTATAGATGACATTTATAAGAAATTCGAAGATTCAATGGACGATGATTTCAATACAGCAGATG
CAATATCAGCTATATTTGAACTTGTAAAGCTTGCTAACTCTAATTCAAGCTCAGATAATTCCAAGGAATACATCGATACT
CTTATGAAGAAGATTACAACTCTCACAGATATTCTTGGACTTAAGACAGACAAGAAGGAAGAAATGCTTGATGAGGATAT
CGAGGCACTTATTGCCGAGAGACAGCAGGCAAGAAAAGATAAGAATTTTGCAAGAGCTGATGAAATTAGAGATGAGTTAT
TAGCAAAGGGAATTGTTCTTAAGGATACAAGAGAGGGCGTAAGATGGAGCAGAGCCTAG

Upstream 100 bases:

>100_bases
CAAGACTTAAGAAGAGACTTGCACACCTTGAAAACAGCGTCAATGAAAGCCGCAGCTCGTATGATGATAACTAAAGTGTG
ATTATGAAAGGAAATCTGAC

Downstream 100 bases:

>100_bases
ATAACGACCTTGTAAAATGCATATATGAAGGCCTGGATATGAAGCATACAGAGCCTTCGCAGTTATCACCTCTTGTTCTT
GCATATATCGGGGACAGCAT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MRIYNTLSRRKEEFKPLEEGKVKMYVCGPTVYNLIHIGNARPMIVFDTVRRYLEYKGYDVNYVSNFTDVDDKIIKKANEE
GVSAEEISTRYIAECKKDMEGMNIKPATTHPLATQEIGGMIDMIQTLIDKGYAYEVNGTVYYRTRKFAEYGKLSKKNIDD
LEAGHRDEAHSLKVTGEDEKEDPLDFVLWKPKKEGEPSWPSPWSDGRPGWHIECSVMSKKYLADEIDIHAGGEDLIFPHH
ENEIAQSEAANGVPFSKYWMHNAFLNIDNKKMSKSLGNFFTVRDISREYDLEVLRFFMLSAHYRNPVNFSHDLMESAKNG
LDRILTAVDNLRHLSENAKDVIMTEDEKKIIASIDDIYKKFEDSMDDDFNTADAISAIFELVKLANSNSSSDNSKEYIDT
LMKKITTLTDILGLKTDKKEEMLDEDIEALIAERQQARKDKNFARADEIRDELLAKGIVLKDTREGVRWSRA

Sequences:

>Translated_472_residues
MRIYNTLSRRKEEFKPLEEGKVKMYVCGPTVYNLIHIGNARPMIVFDTVRRYLEYKGYDVNYVSNFTDVDDKIIKKANEE
GVSAEEISTRYIAECKKDMEGMNIKPATTHPLATQEIGGMIDMIQTLIDKGYAYEVNGTVYYRTRKFAEYGKLSKKNIDD
LEAGHRDEAHSLKVTGEDEKEDPLDFVLWKPKKEGEPSWPSPWSDGRPGWHIECSVMSKKYLADEIDIHAGGEDLIFPHH
ENEIAQSEAANGVPFSKYWMHNAFLNIDNKKMSKSLGNFFTVRDISREYDLEVLRFFMLSAHYRNPVNFSHDLMESAKNG
LDRILTAVDNLRHLSENAKDVIMTEDEKKIIASIDDIYKKFEDSMDDDFNTADAISAIFELVKLANSNSSSDNSKEYIDT
LMKKITTLTDILGLKTDKKEEMLDEDIEALIAERQQARKDKNFARADEIRDELLAKGIVLKDTREGVRWSRA
>Mature_472_residues
MRIYNTLSRRKEEFKPLEEGKVKMYVCGPTVYNLIHIGNARPMIVFDTVRRYLEYKGYDVNYVSNFTDVDDKIIKKANEE
GVSAEEISTRYIAECKKDMEGMNIKPATTHPLATQEIGGMIDMIQTLIDKGYAYEVNGTVYYRTRKFAEYGKLSKKNIDD
LEAGHRDEAHSLKVTGEDEKEDPLDFVLWKPKKEGEPSWPSPWSDGRPGWHIECSVMSKKYLADEIDIHAGGEDLIFPHH
ENEIAQSEAANGVPFSKYWMHNAFLNIDNKKMSKSLGNFFTVRDISREYDLEVLRFFMLSAHYRNPVNFSHDLMESAKNG
LDRILTAVDNLRHLSENAKDVIMTEDEKKIIASIDDIYKKFEDSMDDDFNTADAISAIFELVKLANSNSSSDNSKEYIDT
LMKKITTLTDILGLKTDKKEEMLDEDIEALIAERQQARKDKNFARADEIRDELLAKGIVLKDTREGVRWSRA

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=503, Percent_Identity=30.2186878727634, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI10835051, Length=228, Percent_Identity=41.6666666666667, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI303304987, Length=228, Percent_Identity=41.6666666666667, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI62240992, Length=228, Percent_Identity=41.6666666666667, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI21269877, Length=228, Percent_Identity=41.6666666666667, Blast_Score=192, Evalue=7e-49,
Organism=Escherichia coli, GI1786737, Length=482, Percent_Identity=44.3983402489627, Blast_Score=401, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17509795, Length=329, Percent_Identity=34.0425531914894, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17509797, Length=329, Percent_Identity=34.0425531914894, Blast_Score=168, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6324082, Length=327, Percent_Identity=34.2507645259939, Blast_Score=192, Evalue=8e-50,
Organism=Drosophila melanogaster, GI19922218, Length=513, Percent_Identity=32.5536062378168, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI19922364, Length=330, Percent_Identity=35.4545454545455, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_EUBE2 (C4Z3N5)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002929951.1
- GeneID:   7957603
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00490
- OMA:   DFDALNM
- ProtClustDB:   CLSK2541021
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54258; Mature: 54258

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIYNTLSRRKEEFKPLEEGKVKMYVCGPTVYNLIHIGNARPMIVFDTVRRYLEYKGYDV
CCCHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHEEECCCCCEEEHHHHHHHHHHCCCCE
NYVSNFTDVDDKIIKKANEEGVSAEEISTRYIAECKKDMEGMNIKPATTHPLATQEIGGM
EHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
IDMIQTLIDKGYAYEVNGTVYYRTRKFAEYGKLSKKNIDDLEAGHRDEAHSLKVTGEDEK
HHHHHHHHCCCCEEEECCEEEEEHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEECCCCCC
EDPLDFVLWKPKKEGEPSWPSPWSDGRPGWHIECSVMSKKYLADEIDIHAGGEDLIFPHH
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCEEECCCCCCCCCCC
ENEIAQSEAANGVPFSKYWMHNAFLNIDNKKMSKSLGNFFTVRDISREYDLEVLRFFMLS
CCHHHHHHCCCCCCHHHHHHHHHHEECCHHHHHHHHCCEEEEHHHCHHHHHHHHHHHHHH
AHYRNPVNFSHDLMESAKNGLDRILTAVDNLRHLSENAKDVIMTEDEKKIIASIDDIYKK
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHH
FEDSMDDDFNTADAISAIFELVKLANSNSSSDNSKEYIDTLMKKITTLTDILGLKTDKKE
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
EMLDEDIEALIAERQQARKDKNFARADEIRDELLAKGIVLKDTREGVRWSRA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MRIYNTLSRRKEEFKPLEEGKVKMYVCGPTVYNLIHIGNARPMIVFDTVRRYLEYKGYDV
CCCHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHEEECCCCCEEEHHHHHHHHHHCCCCE
NYVSNFTDVDDKIIKKANEEGVSAEEISTRYIAECKKDMEGMNIKPATTHPLATQEIGGM
EHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
IDMIQTLIDKGYAYEVNGTVYYRTRKFAEYGKLSKKNIDDLEAGHRDEAHSLKVTGEDEK
HHHHHHHHCCCCEEEECCEEEEEHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEECCCCCC
EDPLDFVLWKPKKEGEPSWPSPWSDGRPGWHIECSVMSKKYLADEIDIHAGGEDLIFPHH
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCEEECCCCCCCCCCC
ENEIAQSEAANGVPFSKYWMHNAFLNIDNKKMSKSLGNFFTVRDISREYDLEVLRFFMLS
CCHHHHHHCCCCCCHHHHHHHHHHEECCHHHHHHHHCCEEEEHHHCHHHHHHHHHHHHHH
AHYRNPVNFSHDLMESAKNGLDRILTAVDNLRHLSENAKDVIMTEDEKKIIASIDDIYKK
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHH
FEDSMDDDFNTADAISAIFELVKLANSNSSSDNSKEYIDTLMKKITTLTDILGLKTDKKE
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
EMLDEDIEALIAERQQARKDKNFARADEIRDELLAKGIVLKDTREGVRWSRA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA