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The map label for this gene is folD [H]

Identifier: 238916381

GI number: 238916381

Start: 447688

End: 448530

Strand: Direct

Name: folD [H]

Synonym: EUBELI_00435

Alternate gene names: 238916381

Gene position: 447688-448530 (Clockwise)

Preceding gene: 238916380

Following gene: 238916382

Centisome position: 20.88

GC content: 41.64

Gene sequence:

>843_bases
ATGGAGACACTTAAGGGATTACCGGTTGCTAATGCTATTAATGAGAAGATTATTGAAGAGATGAAGGACTGGAAGGGCGC
AATTCCACATCTTGCAATTGTGCGTGTTGGTGAAAGACCAGATGATATGTCATATGAGCGTGGCGCTACCAAGAAGATGG
AAAAAGTAGGATTCAGATGTACTTCATATACATTTCCTGCTGATATTGATAATGATACTTTCCAGAAAGAATTTGACAAG
ATTAATGAAGATGAGGATATTGACGGAATTCTTCTTTTAAGACCTTTGCCAAAGCATCTTGATGAGAAGGCAATTGAGAA
CAGAATTGCCAGTGTGAAGGATCTGGACGGAATATCACCTATGAATCTTGCAAAGGTATATGCAGGGGATCCGACAGGAT
ATGGTCCATGTACAGCAGAAGCTGTAATTGAGATGTTAGATTATGCAGGAGTTGACTTAAAGGGTAAGAGAGCAGTAGTT
ATCGGAAGAAGTCTTGTTATCGGTAAGCCGGTTGCGATGATGCTTATGAAGAAGAATGCAACAGTAACAGTGTGTCATAC
TAAGACAGTTGATATGCCGGGAGTATGTAAGGGTGCTGAGGTGCTTGTTGCGGCAGCAGGCGTTGCAAAGATGGTTGACA
AATCATTTGTTGCAGATGGTGCAGTTGTAATTGACGTTGGAATAAATGTTGATGAAGACGGCAATCTGTGTGGTGATGTT
GATTTTGAAGATGTTGCAGAGAACGCTTCTGTATGTACACCGGTTCCTGGAGGAGTAGGTTCGGTAACAACATCAGTTCT
TGCAAAACATCTGCTTAAGGCAGCTAAGGCAAGAAGAGCATAA

Upstream 100 bases:

>100_bases
AACAGGATTGCAAAATAAGGAGGAAAATTCCTCTCCTCGCTGGGGGAGACAATGTGCTTAAAAAGCACATGGATTTTGCT
TCGCAAAACAAGGAGGAATA

Downstream 100 bases:

>100_bases
AAAAGTCCTTGCTTTATTGTACATAAGAATGTATTATAATTTGCGGTATGAATTGGTAGCACCAATTTATACCGCAAATT
TTTATTATGGAGAAATGTTA

Product: methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
METLKGLPVANAINEKIIEEMKDWKGAIPHLAIVRVGERPDDMSYERGATKKMEKVGFRCTSYTFPADIDNDTFQKEFDK
INEDEDIDGILLLRPLPKHLDEKAIENRIASVKDLDGISPMNLAKVYAGDPTGYGPCTAEAVIEMLDYAGVDLKGKRAVV
IGRSLVIGKPVAMMLMKKNATVTVCHTKTVDMPGVCKGAEVLVAAAGVAKMVDKSFVADGAVVIDVGINVDEDGNLCGDV
DFEDVAENASVCTPVPGGVGSVTTSVLAKHLLKAAKARRA

Sequences:

>Translated_280_residues
METLKGLPVANAINEKIIEEMKDWKGAIPHLAIVRVGERPDDMSYERGATKKMEKVGFRCTSYTFPADIDNDTFQKEFDK
INEDEDIDGILLLRPLPKHLDEKAIENRIASVKDLDGISPMNLAKVYAGDPTGYGPCTAEAVIEMLDYAGVDLKGKRAVV
IGRSLVIGKPVAMMLMKKNATVTVCHTKTVDMPGVCKGAEVLVAAAGVAKMVDKSFVADGAVVIDVGINVDEDGNLCGDV
DFEDVAENASVCTPVPGGVGSVTTSVLAKHLLKAAKARRA
>Mature_280_residues
METLKGLPVANAINEKIIEEMKDWKGAIPHLAIVRVGERPDDMSYERGATKKMEKVGFRCTSYTFPADIDNDTFQKEFDK
INEDEDIDGILLLRPLPKHLDEKAIENRIASVKDLDGISPMNLAKVYAGDPTGYGPCTAEAVIEMLDYAGVDLKGKRAVV
IGRSLVIGKPVAMMLMKKNATVTVCHTKTVDMPGVCKGAEVLVAAAGVAKMVDKSFVADGAVVIDVGINVDEDGNLCGDV
DFEDVAENASVCTPVPGGVGSVTTSVLAKHLLKAAKARRA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222418558, Length=291, Percent_Identity=36.0824742268041, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI222136639, Length=288, Percent_Identity=33.6805555555556, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI94721354, Length=291, Percent_Identity=34.3642611683849, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI36796743, Length=198, Percent_Identity=25.7575757575758, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786741, Length=246, Percent_Identity=38.2113821138211, Blast_Score=167, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17568735, Length=258, Percent_Identity=36.046511627907, Blast_Score=150, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=40.625, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=36.3636363636364, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322933, Length=323, Percent_Identity=21.0526315789474, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI62472483, Length=284, Percent_Identity=36.6197183098592, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI45551871, Length=284, Percent_Identity=36.6197183098592, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24645718, Length=284, Percent_Identity=36.6197183098592, Blast_Score=177, Evalue=6e-45,
Organism=Drosophila melanogaster, GI17137370, Length=284, Percent_Identity=36.6197183098592, Blast_Score=177, Evalue=6e-45,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=32.8813559322034, Blast_Score=148, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=32.8813559322034, Blast_Score=148, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 29960; Mature: 29960

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLKGLPVANAINEKIIEEMKDWKGAIPHLAIVRVGERPDDMSYERGATKKMEKVGFRC
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHCCHHHHHHHHCCEE
TSYTFPADIDNDTFQKEFDKINEDEDIDGILLLRPLPKHLDEKAIENRIASVKDLDGISP
ECEECCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCC
MNLAKVYAGDPTGYGPCTAEAVIEMLDYAGVDLKGKRAVVIGRSLVIGKPVAMMLMKKNA
CCHHEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECEEECCCHHHHHEEECCC
TVTVCHTKTVDMPGVCKGAEVLVAAAGVAKMVDKSFVADGAVVIDVGINVDEDGNLCGDV
EEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEECCC
DFEDVAENASVCTPVPGGVGSVTTSVLAKHLLKAAKARRA
CHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METLKGLPVANAINEKIIEEMKDWKGAIPHLAIVRVGERPDDMSYERGATKKMEKVGFRC
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHCCHHHHHHHHCCEE
TSYTFPADIDNDTFQKEFDKINEDEDIDGILLLRPLPKHLDEKAIENRIASVKDLDGISP
ECEECCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCC
MNLAKVYAGDPTGYGPCTAEAVIEMLDYAGVDLKGKRAVVIGRSLVIGKPVAMMLMKKNA
CCHHEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECEEECCCHHHHHEEECCC
TVTVCHTKTVDMPGVCKGAEVLVAAAGVAKMVDKSFVADGAVVIDVGINVDEDGNLCGDV
EEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEECCC
DFEDVAENASVCTPVPGGVGSVTTSVLAKHLLKAAKARRA
CHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA