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The map label for this gene is rlmL [H]

Identifier: 238916260

GI number: 238916260

Start: 327789

End: 328679

Strand: Reverse

Name: rlmL [H]

Synonym: EUBELI_00294

Alternate gene names: 238916260

Gene position: 328679-327789 (Counterclockwise)

Preceding gene: 238916301

Following gene: 238916257

Centisome position: 15.33

GC content: 43.55

Gene sequence:

>891_bases
ATGTTTATAGCTGATAACTGGAAAGATTATGAAGTAATAGATACCTCTAAAGGTGAAAAACTTGAACGCTGGGGCAAGTA
TATTCTTGTACGCCCGGACCCACAGGTAATATGGGACACTCCTAAGAAAGAGCGTGGCTGGAAGAATATGAACGGTCATT
ACCACAGAAGTTCTAAGGGCGGCGGAGAATGGGAGTTCTTTGACCTTCCACAGCAGTGGCAGATTCACTACAACAGTCCT
TCATTTGAACAGGAGCTGACATTCAACTTAAAGCCATTCAGCTTCAAGCACACAGGAATATTCCCTGAGCAGGCTGCTAA
CTGGGACTGGTTTGGAAAGATAATCTCTGATGCCAGGAAAAAGCGTGGCAGCGAACCTGTAAAGGTTCTTAACCTGTTTG
CTTATACAGGTGGAGCAACACTTGCATGTGCAGCCGCTGGTGCTAATGTTACACATGTAGATGCTTCTAAGGGTATAGTC
GCATGGGCTAAAGAAAATGCACAGTCTTCAGGACTTATTGACAAGCCTATCCGCTGGATAGTTGATGACTGTGTAAAGTT
TGTAGAAAGAGAGATAAGAAGAGGCAACCATTACGACGCAATCATCATGGATCCACCTTCATATGGACGTGGTCCTAAAG
GAGAAATCTGGAAAATAGAAGAAGCCATACACCCACTTGTTAAGCTTTGTGCACAGCTTCTTTCTGATGAACCATTATTT
TTCCTTATTAATTCATACACTACAGGTCTTCAGCCAGCCGTTCTTACTTATATGTTAAGCACAGAACTTAAGAGCTTTGG
CGGACACTGTGATTCACAGGAAGTAGGTCTTCCTGTTTCAAGTAACGGACTTGTACTTCCATGTGGAGCTTCTGGCCGCT
GGATGAAATAA

Upstream 100 bases:

>100_bases
TTGCACGATTGCAGTTATTAATATTTTCGTATATAATTATTTAATAACAAATGCGGTACCAAACTGCAACATATTTTTAT
ATAGAAAAGGATACTTAATT

Downstream 100 bases:

>100_bases
TTAATACACAACACCATCGAGTGACGGCGATTCTTCGCCGTCATTCACAATTCTAATAACAAGATGATTAGGCAGACATA
TGATTGGCATTAAAGGCTCA

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MFIADNWKDYEVIDTSKGEKLERWGKYILVRPDPQVIWDTPKKERGWKNMNGHYHRSSKGGGEWEFFDLPQQWQIHYNSP
SFEQELTFNLKPFSFKHTGIFPEQAANWDWFGKIISDARKKRGSEPVKVLNLFAYTGGATLACAAAGANVTHVDASKGIV
AWAKENAQSSGLIDKPIRWIVDDCVKFVEREIRRGNHYDAIIMDPPSYGRGPKGEIWKIEEAIHPLVKLCAQLLSDEPLF
FLINSYTTGLQPAVLTYMLSTELKSFGGHCDSQEVGLPVSSNGLVLPCGASGRWMK

Sequences:

>Translated_296_residues
MFIADNWKDYEVIDTSKGEKLERWGKYILVRPDPQVIWDTPKKERGWKNMNGHYHRSSKGGGEWEFFDLPQQWQIHYNSP
SFEQELTFNLKPFSFKHTGIFPEQAANWDWFGKIISDARKKRGSEPVKVLNLFAYTGGATLACAAAGANVTHVDASKGIV
AWAKENAQSSGLIDKPIRWIVDDCVKFVEREIRRGNHYDAIIMDPPSYGRGPKGEIWKIEEAIHPLVKLCAQLLSDEPLF
FLINSYTTGLQPAVLTYMLSTELKSFGGHCDSQEVGLPVSSNGLVLPCGASGRWMK
>Mature_296_residues
MFIADNWKDYEVIDTSKGEKLERWGKYILVRPDPQVIWDTPKKERGWKNMNGHYHRSSKGGGEWEFFDLPQQWQIHYNSP
SFEQELTFNLKPFSFKHTGIFPEQAANWDWFGKIISDARKKRGSEPVKVLNLFAYTGGATLACAAAGANVTHVDASKGIV
AWAKENAQSSGLIDKPIRWIVDDCVKFVEREIRRGNHYDAIIMDPPSYGRGPKGEIWKIEEAIHPLVKLCAQLLSDEPLF
FLINSYTTGLQPAVLTYMLSTELKSFGGHCDSQEVGLPVSSNGLVLPCGASGRWMK

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 33453; Mature: 33453

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIADNWKDYEVIDTSKGEKLERWGKYILVRPDPQVIWDTPKKERGWKNMNGHYHRSSKG
CCCCCCCCCEEEEECCCCHHHHHCCCEEEECCCCCEEECCCCHHCCCCCCCCCEEECCCC
GGEWEFFDLPQQWQIHYNSPSFEQELTFNLKPFSFKHTGIFPEQAANWDWFGKIISDARK
CCCEEEECCCCEEEEEECCCCCCCEEEEECCCCEEECCCCCHHHCCCHHHHHHHHHHHHH
KRGSEPVKVLNLFAYTGGATLACAAAGANVTHVDASKGIVAWAKENAQSSGLIDKPIRWI
HCCCCCHHEEEEEEECCCCEEEEEECCCCEEEEECCCCEEEEECCCCCCCCCCHHHHHHH
VDDCVKFVEREIRRGNHYDAIIMDPPSYGRGPKGEIWKIEEAIHPLVKLCAQLLSDEPLF
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEE
FLINSYTTGLQPAVLTYMLSTELKSFGGHCDSQEVGLPVSSNGLVLPCGASGRWMK
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCEEEECCCCCCCCC
>Mature Secondary Structure
MFIADNWKDYEVIDTSKGEKLERWGKYILVRPDPQVIWDTPKKERGWKNMNGHYHRSSKG
CCCCCCCCCEEEEECCCCHHHHHCCCEEEECCCCCEEECCCCHHCCCCCCCCCEEECCCC
GGEWEFFDLPQQWQIHYNSPSFEQELTFNLKPFSFKHTGIFPEQAANWDWFGKIISDARK
CCCEEEECCCCEEEEEECCCCCCCEEEEECCCCEEECCCCCHHHCCCHHHHHHHHHHHHH
KRGSEPVKVLNLFAYTGGATLACAAAGANVTHVDASKGIVAWAKENAQSSGLIDKPIRWI
HCCCCCHHEEEEEEECCCCEEEEEECCCCEEEEECCCCEEEEECCCCCCCCCCHHHHHHH
VDDCVKFVEREIRRGNHYDAIIMDPPSYGRGPKGEIWKIEEAIHPLVKLCAQLLSDEPLF
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEE
FLINSYTTGLQPAVLTYMLSTELKSFGGHCDSQEVGLPVSSNGLVLPCGASGRWMK
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA