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The map label for this gene is ipk

Identifier: 238916125

GI number: 238916125

Start: 187024

End: 187890

Strand: Direct

Name: ipk

Synonym: EUBELI_00159

Alternate gene names: 238916125

Gene position: 187024-187890 (Clockwise)

Preceding gene: 238916124

Following gene: 238916126

Centisome position: 8.72

GC content: 38.52

Gene sequence:

>867_bases
ATGGAAAGTATCAGATTAAAGGCAAGAGCCAAGATTAATCTTGGACTTGATGTTATTGGAAGACGCGAGAACGGATATCA
TGATGTAAGAATGGTAATGCAGACTGTTGGTTTGTATGACCGTATTATAATGACAAGGATTCCGGAGGAAGAGATAAGAA
TTAAGACTAATATAGGATTTCTACCTGTGAATGAGAATAATCTTGTTTATAAGGCAATTATGCTTATGAAGAATAAGTAT
AAGCTGGATGGTGGAATTGAGGTTGATCTTAATAAATTCATTCCGGTTGCGGCAGGAATGGCTGGAGGTTCAAGTGATGC
GGCATGTGCATTATTTGGAATGAACAGGCTTTTCGAACTTAATGTTCCAATGAGAGAGCTTATGAAGCTTGGTGTTGAGA
TTGGGGCAGATGTTCCATACTGTCTTATGAGAGGAACAGCATTAGCTGAAGGAATTGGAGAGAAGCTTACAAGGCTTCCG
GATATGCCATTTTGTCATATTCTTATAGCAAAGCCTCCGGTAAATGTATCAACTAAGCTTGTGTATGAGAAACTGGATAA
CACAGATGTTAAGTTGCATCCAGATATAGATGGAATAATTGAGGCTATTAAGTTAAAGGATGTTGCACTTGTTGCTTCAA
GAATGGGAAATGTTTTAGAGTCAGTTACAATTCCGTTGTATCCTGTCATTGACAGCATTAAGAAAGATATGATAGAACAT
GGTGCAATTAATGCTATGATGAGTGGCAGTGGACCGACAGTTTTTGGAATATTTCCAGATGAACAATCAATGATAGCCTG
TCAGCAGTTCTTAAGACAGAAGGGCGAGGCAAGGCAGGTGTATACAACGGAAACATTTACACCATAA

Upstream 100 bases:

>100_bases
AACTTTTTAAAGGTGCATTTTAAATATTGTACTTGATTTCATACAATATCTTTAATATAATAGTTTTAAAATGGATTTTG
TATACAAGGTTAGGTAATAT

Downstream 100 bases:

>100_bases
TATATGTGGAAATAATACACATTCAGAGTAATAGAGAAAGGCAAGGTTTTATATGAAAGGTGAACTTAAGATGGATGTTA
ATGACTATCTCCCATTAAGA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MESIRLKARAKINLGLDVIGRRENGYHDVRMVMQTVGLYDRIIMTRIPEEEIRIKTNIGFLPVNENNLVYKAIMLMKNKY
KLDGGIEVDLNKFIPVAAGMAGGSSDAACALFGMNRLFELNVPMRELMKLGVEIGADVPYCLMRGTALAEGIGEKLTRLP
DMPFCHILIAKPPVNVSTKLVYEKLDNTDVKLHPDIDGIIEAIKLKDVALVASRMGNVLESVTIPLYPVIDSIKKDMIEH
GAINAMMSGSGPTVFGIFPDEQSMIACQQFLRQKGEARQVYTTETFTP

Sequences:

>Translated_288_residues
MESIRLKARAKINLGLDVIGRRENGYHDVRMVMQTVGLYDRIIMTRIPEEEIRIKTNIGFLPVNENNLVYKAIMLMKNKY
KLDGGIEVDLNKFIPVAAGMAGGSSDAACALFGMNRLFELNVPMRELMKLGVEIGADVPYCLMRGTALAEGIGEKLTRLP
DMPFCHILIAKPPVNVSTKLVYEKLDNTDVKLHPDIDGIIEAIKLKDVALVASRMGNVLESVTIPLYPVIDSIKKDMIEH
GAINAMMSGSGPTVFGIFPDEQSMIACQQFLRQKGEARQVYTTETFTP
>Mature_288_residues
MESIRLKARAKINLGLDVIGRRENGYHDVRMVMQTVGLYDRIIMTRIPEEEIRIKTNIGFLPVNENNLVYKAIMLMKNKY
KLDGGIEVDLNKFIPVAAGMAGGSSDAACALFGMNRLFELNVPMRELMKLGVEIGADVPYCLMRGTALAEGIGEKLTRLP
DMPFCHILIAKPPVNVSTKLVYEKLDNTDVKLHPDIDGIIEAIKLKDVALVASRMGNVLESVTIPLYPVIDSIKKDMIEH
GAINAMMSGSGPTVFGIFPDEQSMIACQQFLRQKGEARQVYTTETFTP

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=272, Percent_Identity=27.2058823529412, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_EUBE2 (C4Z205)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002929642.1
- ProteinModelPortal:   C4Z205
- GeneID:   7958324
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00159
- OMA:   RIYNGLR
- ProtClustDB:   PRK00128
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31946; Mature: 31946

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIRLKARAKINLGLDVIGRRENGYHDVRMVMQTVGLYDRIIMTRIPEEEIRIKTNIGF
CCCCEEEEEEEEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCEE
LPVNENNLVYKAIMLMKNKYKLDGGIEVDLNKFIPVAAGMAGGSSDAACALFGMNRLFEL
EEECCCCHHHHHHHHHHCCEECCCCEEEEHHHCCHHHCCCCCCCCCCEEEEECCCCEEEE
NVPMRELMKLGVEIGADVPYCLMRGTALAEGIGEKLTRLPDMPFCHILIAKPPVNVSTKL
CCCHHHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHH
VYEKLDNTDVKLHPDIDGIIEAIKLKDVALVASRMGNVLESVTIPLYPVIDSIKKDMIEH
HHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GAINAMMSGSGPTVFGIFPDEQSMIACQQFLRQKGEARQVYTTETFTP
CCCEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCC
>Mature Secondary Structure
MESIRLKARAKINLGLDVIGRRENGYHDVRMVMQTVGLYDRIIMTRIPEEEIRIKTNIGF
CCCCEEEEEEEEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCEE
LPVNENNLVYKAIMLMKNKYKLDGGIEVDLNKFIPVAAGMAGGSSDAACALFGMNRLFEL
EEECCCCHHHHHHHHHHCCEECCCCEEEEHHHCCHHHCCCCCCCCCCEEEEECCCCEEEE
NVPMRELMKLGVEIGADVPYCLMRGTALAEGIGEKLTRLPDMPFCHILIAKPPVNVSTKL
CCCHHHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHH
VYEKLDNTDVKLHPDIDGIIEAIKLKDVALVASRMGNVLESVTIPLYPVIDSIKKDMIEH
HHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GAINAMMSGSGPTVFGIFPDEQSMIACQQFLRQKGEARQVYTTETFTP
CCCEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA