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The map label for this gene is tycA [H]

Identifier: 238916081

GI number: 238916081

Start: 128469

End: 130028

Strand: Direct

Name: tycA [H]

Synonym: EUBELI_00114

Alternate gene names: 238916081

Gene position: 128469-130028 (Clockwise)

Preceding gene: 238916080

Following gene: 238916082

Centisome position: 5.99

GC content: 38.72

Gene sequence:

>1560_bases
ATGGAAAAGAATGTATTAGAGTTTCTTGAAAACTCACAGAGAATGTATGGGGAAAAGACAGCGTGTGCGGACATGAATAA
TGAACTTACATACACACAGCTTATGAATCAGGCAGAGTTCATAGGAAGCTGTCTCTCACTTAAGATTAAGCCTACACAGG
GAGTTCCTGTGTATATGGATAAGACATGTAATACATTATCTTTATTTATGGGAATTGTGTATGCAGGCGGATATTACTGT
ATGCTTGATACAACTCATCCGGCTGAGAGAATTAATCTTATATTAGATACGATCCAGGCAGATATTCTGGTTGTAGATAA
TAAGTCAGCCAAGAAAGCAGAAAAGCTCGGATTTCAGGGCGAGATTATTAATCTTGACGAATTGCTTGAAGAGTATGACG
GACAGGCTGTGGATTCAGAGGCTCAGGAGAGACTGGAAAAGATAAGAGAACAGGCACTTGATACAGATCCTCTGTATTCA
ATATTTACATCAGGTTCAACAGGAGTTCCTAAGGGAGTTATTGTCAATCATCGTTCAACAATAGATTTTATTGATTGCTT
TACGGAAACATTTGGAATTACTAATGAGGATGTTATAGGTAATCAGGCACCTTTTGACTTTGATGTATCAGTTAAGGATA
TATATTCAAGTTTAAAGACAGGCGCAACCTTACAGCTTATTCCTAAGATGTGCTTTTCGTTCCCAACCAAACTGCTTGAT
TTCCTTGATGACAGAAAGGTTACAACTATTATATGGGCTGTATCAGCGTTATGTATTGTAACAACTCTTAATGGATTCGA
GTATAAGAGGCCATCTTCACTTAACAAGATTATGTTCAGCGGAGAGGTTATGCCTATAAAGCATCTTAATGCATGGAGAG
TGCAGTATCCGGATGCAATGTTTGTTAATCTTTACGGACCAACAGAGATAACATGTAATTGTACATATTATATTATTGAC
AGAGAGTTTGGTCTTGACGAGAAGCTTCCGATGGGAAAGCCATTTGCCAATGAAAGAGTATTCCTGTTAGATGATGAGGA
TAAGCTTGTTGATGCAGGCAGACCAGGCGAGTTAGGAGAAATATGTGTATCTGGAACAGCTGTAACTATGGGGTATCTGC
GTAATCCTGAAAAGACAGCAGCGGCATTTGTACAGAATCCTCTTAATGACAGATATCTGGAAACTATATACCGCACAGGT
GATCTTGGTTACTATAGTGAAGATGGTGAACTTTTCTTCTCATCAAGAAAGGACTTCCAGATTAAGCATATGGGACACAG
AATCGAGCTGGGCGAAATTGAGACATACATGAATGCTATAGAGGGACTTCTAAGAAGCTGCTGTATATTTGATACAGACG
AGAATAAGATTCTCGGATTCTTTGAGATAGAAGGAGCAAGTGAGCTTGACAGAAAGGGCGTAACACATGCTCTCAAAGAT
AAAGTGCCACAGTGGATGATTCCAAATGTGCTCGTTAAGGTTGAAACAATGCCGATTACTAAGAATGGTAAAATTGACAG
AAAAGAACTTATGAAGATATATAAAGAACAGGAGGCATAG

Upstream 100 bases:

>100_bases
AAAGTTTATTGCGCAGGTTGTGGTAATTATACTTAATTATGTTATAAGTAAGCTGCTTGTATTCAGAAAAGATAAGAGTG
AGAAGTAAAAGGAGAAGGTA

Downstream 100 bases:

>100_bases
GATGGTAGGAGTTAATGAATTAAAAGAGATTGCCATAAAATGTGGAACACCATCGTATATATTTGATACAGATGAGCTAT
GTGCAAGGATTGATGCCATG

Product: hypothetical protein

Products: NA

Alternate protein names: Tyrocidine synthase I; ATP-dependent D-phenylalanine adenylase; D-PheA; D-phenylalanine activase; Phenylalanine racemase [ATP-hydrolyzing] [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MEKNVLEFLENSQRMYGEKTACADMNNELTYTQLMNQAEFIGSCLSLKIKPTQGVPVYMDKTCNTLSLFMGIVYAGGYYC
MLDTTHPAERINLILDTIQADILVVDNKSAKKAEKLGFQGEIINLDELLEEYDGQAVDSEAQERLEKIREQALDTDPLYS
IFTSGSTGVPKGVIVNHRSTIDFIDCFTETFGITNEDVIGNQAPFDFDVSVKDIYSSLKTGATLQLIPKMCFSFPTKLLD
FLDDRKVTTIIWAVSALCIVTTLNGFEYKRPSSLNKIMFSGEVMPIKHLNAWRVQYPDAMFVNLYGPTEITCNCTYYIID
REFGLDEKLPMGKPFANERVFLLDDEDKLVDAGRPGELGEICVSGTAVTMGYLRNPEKTAAAFVQNPLNDRYLETIYRTG
DLGYYSEDGELFFSSRKDFQIKHMGHRIELGEIETYMNAIEGLLRSCCIFDTDENKILGFFEIEGASELDRKGVTHALKD
KVPQWMIPNVLVKVETMPITKNGKIDRKELMKIYKEQEA

Sequences:

>Translated_519_residues
MEKNVLEFLENSQRMYGEKTACADMNNELTYTQLMNQAEFIGSCLSLKIKPTQGVPVYMDKTCNTLSLFMGIVYAGGYYC
MLDTTHPAERINLILDTIQADILVVDNKSAKKAEKLGFQGEIINLDELLEEYDGQAVDSEAQERLEKIREQALDTDPLYS
IFTSGSTGVPKGVIVNHRSTIDFIDCFTETFGITNEDVIGNQAPFDFDVSVKDIYSSLKTGATLQLIPKMCFSFPTKLLD
FLDDRKVTTIIWAVSALCIVTTLNGFEYKRPSSLNKIMFSGEVMPIKHLNAWRVQYPDAMFVNLYGPTEITCNCTYYIID
REFGLDEKLPMGKPFANERVFLLDDEDKLVDAGRPGELGEICVSGTAVTMGYLRNPEKTAAAFVQNPLNDRYLETIYRTG
DLGYYSEDGELFFSSRKDFQIKHMGHRIELGEIETYMNAIEGLLRSCCIFDTDENKILGFFEIEGASELDRKGVTHALKD
KVPQWMIPNVLVKVETMPITKNGKIDRKELMKIYKEQEA
>Mature_519_residues
MEKNVLEFLENSQRMYGEKTACADMNNELTYTQLMNQAEFIGSCLSLKIKPTQGVPVYMDKTCNTLSLFMGIVYAGGYYC
MLDTTHPAERINLILDTIQADILVVDNKSAKKAEKLGFQGEIINLDELLEEYDGQAVDSEAQERLEKIREQALDTDPLYS
IFTSGSTGVPKGVIVNHRSTIDFIDCFTETFGITNEDVIGNQAPFDFDVSVKDIYSSLKTGATLQLIPKMCFSFPTKLLD
FLDDRKVTTIIWAVSALCIVTTLNGFEYKRPSSLNKIMFSGEVMPIKHLNAWRVQYPDAMFVNLYGPTEITCNCTYYIID
REFGLDEKLPMGKPFANERVFLLDDEDKLVDAGRPGELGEICVSGTAVTMGYLRNPEKTAAAFVQNPLNDRYLETIYRTG
DLGYYSEDGELFFSSRKDFQIKHMGHRIELGEIETYMNAIEGLLRSCCIFDTDENKILGFFEIEGASELDRKGVTHALKD
KVPQWMIPNVLVKVETMPITKNGKIDRKELMKIYKEQEA

Specific function: In the first step of peptide synthesis this enzyme activates phenylalanine and racemizes it to the D-isomer [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=561, Percent_Identity=25.1336898395722, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI38505220, Length=447, Percent_Identity=23.7136465324385, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI122937307, Length=372, Percent_Identity=25, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI42544132, Length=380, Percent_Identity=24.2105263157895, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1786801, Length=512, Percent_Identity=27.734375, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI145693145, Length=374, Percent_Identity=25.1336898395722, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17550940, Length=362, Percent_Identity=26.5193370165746, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17556356, Length=498, Percent_Identity=25.1004016064257, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=373, Percent_Identity=24.9329758713137, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319591, Length=581, Percent_Identity=22.3752151462995, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319699, Length=182, Percent_Identity=24.1758241758242, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24648676, Length=521, Percent_Identity=26.679462571977, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24582852, Length=435, Percent_Identity=24.5977011494253, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648257, Length=368, Percent_Identity=23.6413043478261, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21356441, Length=453, Percent_Identity=20.0883002207506, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24653035, Length=460, Percent_Identity=22.3913043478261, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21358303, Length=472, Percent_Identity=22.0338983050847, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648253, Length=431, Percent_Identity=21.3457076566125, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281365686, Length=290, Percent_Identity=23.1034482758621, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648255, Length=430, Percent_Identity=21.3953488372093, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: =5.1.1.11 [H]

Molecular weight: Translated: 58725; Mature: 58725

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKNVLEFLENSQRMYGEKTACADMNNELTYTQLMNQAEFIGSCLSLKIKPTQGVPVYMD
CCCHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEEC
KTCNTLSLFMGIVYAGGYYCMLDTTHPAERINLILDTIQADILVVDNKSAKKAEKLGFQG
CCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCC
EIINLDELLEEYDGQAVDSEAQERLEKIREQALDTDPLYSIFTSGSTGVPKGVIVNHRST
CEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCEEEECCCC
IDFIDCFTETFGITNEDVIGNQAPFDFDVSVKDIYSSLKTGATLQLIPKMCFSFPTKLLD
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
FLDDRKVTTIIWAVSALCIVTTLNGFEYKRPSSLNKIMFSGEVMPIKHLNAWRVQYPDAM
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCEEEEECCCEEEEECCCEE
FVNLYGPTEITCNCTYYIIDREFGLDEKLPMGKPFANERVFLLDDEDKLVDAGRPGELGE
EEEEECCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCCCCCHH
ICVSGTAVTMGYLRNPEKTAAAFVQNPLNDRYLETIYRTGDLGYYSEDGELFFSSRKDFQ
EEECCCEEEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCEECCCCCEEECCCCCEE
IKHMGHRIELGEIETYMNAIEGLLRSCCIFDTDENKILGFFEIEGASELDRKGVTHALKD
EECCCCEEEHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCHHHHHHCCHHHHHH
KVPQWMIPNVLVKVETMPITKNGKIDRKELMKIYKEQEA
CCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MEKNVLEFLENSQRMYGEKTACADMNNELTYTQLMNQAEFIGSCLSLKIKPTQGVPVYMD
CCCHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEEC
KTCNTLSLFMGIVYAGGYYCMLDTTHPAERINLILDTIQADILVVDNKSAKKAEKLGFQG
CCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCC
EIINLDELLEEYDGQAVDSEAQERLEKIREQALDTDPLYSIFTSGSTGVPKGVIVNHRST
CEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCEEEECCCC
IDFIDCFTETFGITNEDVIGNQAPFDFDVSVKDIYSSLKTGATLQLIPKMCFSFPTKLLD
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
FLDDRKVTTIIWAVSALCIVTTLNGFEYKRPSSLNKIMFSGEVMPIKHLNAWRVQYPDAM
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCEEEEECCCEEEEECCCEE
FVNLYGPTEITCNCTYYIIDREFGLDEKLPMGKPFANERVFLLDDEDKLVDAGRPGELGE
EEEEECCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCCCCCHH
ICVSGTAVTMGYLRNPEKTAAAFVQNPLNDRYLETIYRTGDLGYYSEDGELFFSSRKDFQ
EEECCCEEEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCEECCCCCEEECCCCCEE
IKHMGHRIELGEIETYMNAIEGLLRSCCIFDTDENKILGFFEIEGASELDRKGVTHALKD
EECCCCEEEHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCHHHHHHCCHHHHHH
KVPQWMIPNVLVKVETMPITKNGKIDRKELMKIYKEQEA
CCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3267240; 9352938; 3032912; 8193142 [H]