Click here to switch to the map view.

The map label for this gene is rffG [H]

Identifier: 238916053

GI number: 238916053

Start: 92859

End: 93878

Strand: Reverse

Name: rffG [H]

Synonym: EUBELI_00086

Alternate gene names: 238916053

Gene position: 93878-92859 (Counterclockwise)

Preceding gene: 238916054

Following gene: 238916052

Centisome position: 4.38

GC content: 41.27

Gene sequence:

>1020_bases
ATGACAATTATTGTTACCGGTGGAGCCGGATTTATCGGAAGTAACTTTATATTTCACATGTTAAACAAATACCCAGACTA
CAGAATCATCTGTCTTGACTGCCTCACATATGCAGGTAATCTTTCAACTCTTGAGCCTGTTATGTCTAACCCTAACTTCA
GATTCGTTAAGGAAAGCATCACAGACCGCGAAGCTGTTTACAAACTTTTCGAAGAAGAGCATCCAGATATGGTTGTAAAC
TTCGCTGCTGAATCACACGTTGACCGTTCTATCGAGAACCCGGAAGTTTTCCTTATAACTAATATTCTTGGTACACAGGT
TCTTATGGATGCCTGCAGAAAGTACGGTATTCAGAGATACCATCAGGTATCTACTGATGAAGTATACGGCGATCTTCCAC
TTGACAGACCTGACCTTTTCTTCACAGAAGAGACACCAATCCATACATCAAGCCCATACAGCTCATCAAAGGCTGGCGCA
GATTTACTTGTTCTTGCTTACCACAGAACTTATGGACTTCCTGTTACTATTTCAAGATGCTCTAATAACTATGGACCATA
CCACTTCCCAGAGAAGCTTATCCCTCTTATGATTGCTAATGCTCTTAACGATAAGCCACTTCCAGTTTATGGCGAAGGTC
TTAACGTTCGTGACTGGCTCTATGTTGAGGATCACTGCAAGGCTATCGACCTTATCATCCACAAGGGACGTGTTGGTGAA
GTATACAACGTTGGTGGACACAACGAGAAGAGAAATATTGATATCGTTAAGCTTATCTGCAAGGAGCTTGGCAAGCCTGA
ATCACTTATCGTTCATGTAGGTGACAGAAAGGGACATGATATGCGTTACGCTATCGACCCAACTAAGATTCACAATGAAT
TAGGATGGCTTCCAGAAACTAAGTTTGAAGATGGTATCAAGAAGACAATTCAGTGGTATCTTGATAATAAGGAATGGTGG
GAGACAATCATTTCTGGCGAATATCAGAACTACTATGAGAAAATGTATAGCAACCGCTAA

Upstream 100 bases:

>100_bases
TAGACGGCAAATATGTCGACAAGCTTCATGAATAATTAAGTACATTTAGTTAAAACATTTTTCATATAAGAACACATTTT
AAATATTAAGGAGATTAATT

Downstream 100 bases:

>100_bases
TTAACACATTTATTCTGTAAGGAGATTTGACATGAAGATATTAGTTACCGGCGTTGGCGGTCAGCTTGGCCACGACGTTA
TGAATGAACTGATAGGCAGA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTIIVTGGAGFIGSNFIFHMLNKYPDYRIICLDCLTYAGNLSTLEPVMSNPNFRFVKESITDREAVYKLFEEEHPDMVVN
FAAESHVDRSIENPEVFLITNILGTQVLMDACRKYGIQRYHQVSTDEVYGDLPLDRPDLFFTEETPIHTSSPYSSSKAGA
DLLVLAYHRTYGLPVTISRCSNNYGPYHFPEKLIPLMIANALNDKPLPVYGEGLNVRDWLYVEDHCKAIDLIIHKGRVGE
VYNVGGHNEKRNIDIVKLICKELGKPESLIVHVGDRKGHDMRYAIDPTKIHNELGWLPETKFEDGIKKTIQWYLDNKEWW
ETIISGEYQNYYEKMYSNR

Sequences:

>Translated_339_residues
MTIIVTGGAGFIGSNFIFHMLNKYPDYRIICLDCLTYAGNLSTLEPVMSNPNFRFVKESITDREAVYKLFEEEHPDMVVN
FAAESHVDRSIENPEVFLITNILGTQVLMDACRKYGIQRYHQVSTDEVYGDLPLDRPDLFFTEETPIHTSSPYSSSKAGA
DLLVLAYHRTYGLPVTISRCSNNYGPYHFPEKLIPLMIANALNDKPLPVYGEGLNVRDWLYVEDHCKAIDLIIHKGRVGE
VYNVGGHNEKRNIDIVKLICKELGKPESLIVHVGDRKGHDMRYAIDPTKIHNELGWLPETKFEDGIKKTIQWYLDNKEWW
ETIISGEYQNYYEKMYSNR
>Mature_338_residues
TIIVTGGAGFIGSNFIFHMLNKYPDYRIICLDCLTYAGNLSTLEPVMSNPNFRFVKESITDREAVYKLFEEEHPDMVVNF
AAESHVDRSIENPEVFLITNILGTQVLMDACRKYGIQRYHQVSTDEVYGDLPLDRPDLFFTEETPIHTSSPYSSSKAGAD
LLVLAYHRTYGLPVTISRCSNNYGPYHFPEKLIPLMIANALNDKPLPVYGEGLNVRDWLYVEDHCKAIDLIIHKGRVGEV
YNVGGHNEKRNIDIVKLICKELGKPESLIVHVGDRKGHDMRYAIDPTKIHNELGWLPETKFEDGIKKTIQWYLDNKEWWE
TIISGEYQNYYEKMYSNR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=327, Percent_Identity=38.8379204892966, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI42516563, Length=320, Percent_Identity=25.9375, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI4504031, Length=349, Percent_Identity=24.9283667621776, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI56237023, Length=353, Percent_Identity=25.7790368271955, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI56118217, Length=353, Percent_Identity=25.7790368271955, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI189083684, Length=353, Percent_Identity=25.7790368271955, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI48994969, Length=347, Percent_Identity=53.6023054755043, Blast_Score=376, Evalue=1e-106,
Organism=Escherichia coli, GI1788353, Length=363, Percent_Identity=50.4132231404959, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1786974, Length=336, Percent_Identity=25.297619047619, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788366, Length=302, Percent_Identity=26.4900662251656, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788365, Length=287, Percent_Identity=25.0871080139373, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=322, Percent_Identity=35.4037267080745, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=320, Percent_Identity=31.5625, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71982035, Length=359, Percent_Identity=26.4623955431755, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71982038, Length=361, Percent_Identity=26.3157894736842, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17539532, Length=314, Percent_Identity=25.1592356687898, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI133901786, Length=245, Percent_Identity=26.1224489795918, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=245, Percent_Identity=26.1224489795918, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=245, Percent_Identity=26.1224489795918, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=245, Percent_Identity=26.1224489795918, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=245, Percent_Identity=26.1224489795918, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=339, Percent_Identity=23.3038348082596, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=344, Percent_Identity=25, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19923002, Length=347, Percent_Identity=29.971181556196, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356223, Length=314, Percent_Identity=25.796178343949, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24158427, Length=332, Percent_Identity=23.1927710843373, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39011; Mature: 38880

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIVTGGAGFIGSNFIFHMLNKYPDYRIICLDCLTYAGNLSTLEPVMSNPNFRFVKESI
CEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHHHHCCCCCEEHHHHH
TDREAVYKLFEEEHPDMVVNFAAESHVDRSIENPEVFLITNILGTQVLMDACRKYGIQRY
HHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
HQVSTDEVYGDLPLDRPDLFFTEETPIHTSSPYSSSKAGADLLVLAYHRTYGLPVTISRC
HCCCCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEEHHCCCCEEEEEC
SNNYGPYHFPEKLIPLMIANALNDKPLPVYGEGLNVRDWLYVEDHCKAIDLIIHKGRVGE
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEHHHCCEEEEEEECCCCCC
VYNVGGHNEKRNIDIVKLICKELGKPESLIVHVGDRKGHDMRYAIDPTKIHNELGWLPET
EEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECHHHHHHHHCCCCCC
KFEDGIKKTIQWYLDNKEWWETIISGEYQNYYEKMYSNR
HHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TIIVTGGAGFIGSNFIFHMLNKYPDYRIICLDCLTYAGNLSTLEPVMSNPNFRFVKESI
EEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHHHHCCCCCEEHHHHH
TDREAVYKLFEEEHPDMVVNFAAESHVDRSIENPEVFLITNILGTQVLMDACRKYGIQRY
HHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
HQVSTDEVYGDLPLDRPDLFFTEETPIHTSSPYSSSKAGADLLVLAYHRTYGLPVTISRC
HCCCCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEEHHCCCCEEEEEC
SNNYGPYHFPEKLIPLMIANALNDKPLPVYGEGLNVRDWLYVEDHCKAIDLIIHKGRVGE
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEHHHCCEEEEEEECCCCCC
VYNVGGHNEKRNIDIVKLICKELGKPESLIVHVGDRKGHDMRYAIDPTKIHNELGWLPET
EEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECHHHHHHHHCCCCCC
KFEDGIKKTIQWYLDNKEWWETIISGEYQNYYEKMYSNR
HHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]