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The map label for this gene is spsK [H]

Identifier: 238916052

GI number: 238916052

Start: 91910

End: 92827

Strand: Reverse

Name: spsK [H]

Synonym: EUBELI_00085

Alternate gene names: 238916052

Gene position: 92827-91910 (Counterclockwise)

Preceding gene: 238916053

Following gene: 238916051

Centisome position: 4.33

GC content: 42.27

Gene sequence:

>918_bases
ATGAAGATATTAGTTACCGGCGTTGGCGGTCAGCTTGGCCACGACGTTATGAATGAACTGATAGGCAGAGGTCATACAGG
CGTTGGCAGCGACATCGCACCTGAATATTCAGGAGTTGCTGATGGAAGCGCTGTCACAACAGCCGAATATGTACCTATGG
ATATAACTGATGCTGCCCAGGTTTCTGAAGTTATCACTAAGGTTTCACCTGATGTTGTAATTCACTGCGCAGCATGGACA
GCCGTTGACGCTGCTGAAGATGATGATAAGAAAGCCAAGGTTAAGGCTATCAATGCAGATGGTACTCAGAATATTGCCAA
TGTCTGCAAACAGCTTGACTGCAAGATGGTATACATCAGTACTGATTATGTATTTGATGGTCAGGGTGAAACACCTTGGG
AACCAGACTGCAAGGACTACAAGCCTCTTAATGTATATGGAGAGACTAAGCTTGCCGGAGAACTTGCTGTTTCTAACACT
CTTGATAAATACTTTATCGTGCGTATTGCATGGGTATTTGGTGTTAATGGAAAGAACTTTATTAAGACTATGCTTAATGT
AGGAAAGACTCATGACGAGGTTCGTGTTGTCAACGACCAGATTGGTACACCAACTTACACATTAGATCTTGCAAGACTTC
TTGTTGATATGGTTGAAACTGACAAATACGGTTATTACCATGCAACTAATGAAGGTGGATACATTTCATGGTATGACTTT
ACTAAGGAAATCTATGCACAGGCAGGTCTTTCTACTAAGGTTACACCTGTAACTACTGCTGAATACGGCATTTCTAAAGC
TGCAAGACCTTTTAACAGCCGTTTAGATAAGAGCAAGCTTACTGCTAACGGATTCACTCCGCTTCCAACATGGCAGGATG
CTCTTCACAGATTCTTAAAGGAAATCGGTGAGATTTAA

Upstream 100 bases:

>100_bases
GAATGGTGGGAGACAATCATTTCTGGCGAATATCAGAACTACTATGAGAAAATGTATAGCAACCGCTAATTAACACATTT
ATTCTGTAAGGAGATTTGAC

Downstream 100 bases:

>100_bases
TATTTCAAAGGAGATTTTATCATGGGACAGATTACAATTGAAAAGAATGTCGCAGGTATCGAAGGACTTTGTGTCATCAC
TCCTGCTGTTCACGGCGATA

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKILVTGVGGQLGHDVMNELIGRGHTGVGSDIAPEYSGVADGSAVTTAEYVPMDITDAAQVSEVITKVSPDVVIHCAAWT
AVDAAEDDDKKAKVKAINADGTQNIANVCKQLDCKMVYISTDYVFDGQGETPWEPDCKDYKPLNVYGETKLAGELAVSNT
LDKYFIVRIAWVFGVNGKNFIKTMLNVGKTHDEVRVVNDQIGTPTYTLDLARLLVDMVETDKYGYYHATNEGGYISWYDF
TKEIYAQAGLSTKVTPVTTAEYGISKAARPFNSRLDKSKLTANGFTPLPTWQDALHRFLKEIGEI

Sequences:

>Translated_305_residues
MKILVTGVGGQLGHDVMNELIGRGHTGVGSDIAPEYSGVADGSAVTTAEYVPMDITDAAQVSEVITKVSPDVVIHCAAWT
AVDAAEDDDKKAKVKAINADGTQNIANVCKQLDCKMVYISTDYVFDGQGETPWEPDCKDYKPLNVYGETKLAGELAVSNT
LDKYFIVRIAWVFGVNGKNFIKTMLNVGKTHDEVRVVNDQIGTPTYTLDLARLLVDMVETDKYGYYHATNEGGYISWYDF
TKEIYAQAGLSTKVTPVTTAEYGISKAARPFNSRLDKSKLTANGFTPLPTWQDALHRFLKEIGEI
>Mature_305_residues
MKILVTGVGGQLGHDVMNELIGRGHTGVGSDIAPEYSGVADGSAVTTAEYVPMDITDAAQVSEVITKVSPDVVIHCAAWT
AVDAAEDDDKKAKVKAINADGTQNIANVCKQLDCKMVYISTDYVFDGQGETPWEPDCKDYKPLNVYGETKLAGELAVSNT
LDKYFIVRIAWVFGVNGKNFIKTMLNVGKTHDEVRVVNDQIGTPTYTLDLARLLVDMVETDKYGYYHATNEGGYISWYDF
TKEIYAQAGLSTKVTPVTTAEYGISKAARPFNSRLDKSKLTANGFTPLPTWQDALHRFLKEIGEI

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=294, Percent_Identity=28.9115646258503, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI11034825, Length=294, Percent_Identity=28.9115646258503, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788352, Length=314, Percent_Identity=34.7133757961783, Blast_Score=167, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 33368; Mature: 33368

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGVGGQLGHDVMNELIGRGHTGVGSDIAPEYSGVADGSAVTTAEYVPMDITDAAQ
CEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHH
VSEVITKVSPDVVIHCAAWTAVDAAEDDDKKAKVKAINADGTQNIANVCKQLDCKMVYIS
HHHHHHHCCCCEEEEEEHHEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEE
TDYVFDGQGETPWEPDCKDYKPLNVYGETKLAGELAVSNTLDKYFIVRIAWVFGVNGKNF
ECEEECCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHEEEHHCCCCHHH
IKTMLNVGKTHDEVRVVNDQIGTPTYTLDLARLLVDMVETDKYGYYHATNEGGYISWYDF
HHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEHHHH
TKEIYAQAGLSTKVTPVTTAEYGISKAARPFNSRLDKSKLTANGFTPLPTWQDALHRFLK
HHHHHHHCCCCCCCCEEEEHHHHHHHHCCCHHHHCCHHHCCCCCCCCCCCHHHHHHHHHH
EIGEI
HHCCC
>Mature Secondary Structure
MKILVTGVGGQLGHDVMNELIGRGHTGVGSDIAPEYSGVADGSAVTTAEYVPMDITDAAQ
CEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHH
VSEVITKVSPDVVIHCAAWTAVDAAEDDDKKAKVKAINADGTQNIANVCKQLDCKMVYIS
HHHHHHHCCCCEEEEEEHHEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEE
TDYVFDGQGETPWEPDCKDYKPLNVYGETKLAGELAVSNTLDKYFIVRIAWVFGVNGKNF
ECEEECCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHEEEHHCCCCHHH
IKTMLNVGKTHDEVRVVNDQIGTPTYTLDLARLLVDMVETDKYGYYHATNEGGYISWYDF
HHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEHHHH
TKEIYAQAGLSTKVTPVTTAEYGISKAARPFNSRLDKSKLTANGFTPLPTWQDALHRFLK
HHHHHHHCCCCCCCCEEEEHHHHHHHHCCCHHHHCCHHHCCCCCCCCCCCHHHHHHHHHH
EIGEI
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]