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The map label for this gene is gyrB [H]

Identifier: 238915981

GI number: 238915981

Start: 4124

End: 6058

Strand: Direct

Name: gyrB [H]

Synonym: EUBELI_00005

Alternate gene names: 238915981

Gene position: 4124-6058 (Clockwise)

Preceding gene: 238915980

Following gene: 238915982

Centisome position: 0.19

GC content: 37.62

Gene sequence:

>1935_bases
GTGGCAGATCAGAATGCAGAGAAGAATTATGGTGGAGACCAGATTCAGGTACTCGAAGGTCTTGAAGCTGTAAGAAAAAG
ACCTGGTATGTATATTGGAAGTACATCAGGAAGAGGACTTCATCATCTTGTGTATGAGATAGTTGATAATGCAATTGACG
AAGCTTTAGCAGGTTTTTGTACACATATTGAGGTTATGATTAATAAAGATAACTCTATCACTGTAATTGATAATGGTAGA
GGTGTACCTGTTGATATTAATCATAAGACAGGAAGACCTGCCATTGAAGTTGTATATACCGTTCTTCATGCCGGAGGTAA
ATTCGGTGGTGGAGGATATAAGGTGTCAGGAGGACTTCACGGTGTTGGTGCATCTGTAGTTAATGCTTTGTCTGAATGGC
TTGAAGTTCAGGTAAAGAGAAATGGACACATTTATCAGCAGAGATACGAAAGAGGAAAGATATGTTATGACCTTAAGATA
GTAGGTGACTGCGATATTGAGGATACAGGAACTCAGGTTACTTTTCTTCCTGATTCGGAGATTTTTCAGGAAACAACTGT
ATTTGAATTTGATATTCTTATGCACAGATTAAGAGAAATGGCATTTCTTACTAAGGGAATCAAGATTACTCTTACAGATT
TAAGAGAAGGTCTTGAGCAGCAGAAAGTATTCCATTATGAAGGTGGTATAAAGGAATTTGTACAGTATCTTAATAAGAGT
AAAGAACCTTTATACAGCCAGATTATTTATTGTGAAGGTGTGAAAGACAATGTTCAGGTTGAAGTAGCATTCCAGCATAA
TGATGGATACAATGAGGTTGTCGATTCATTTGTTAATAATATTAAGACACCTGAAGGTGGTACACATCTGACTGGTTTCA
GAAATTCACTGACTAAGACTTTTAATGATTATGCAAGAAAGAATAAGATTCTTAAGGATAGTGATCAGGGACTTTCAGGT
GATGATATAAGAGAAGGTCTTACAGCAATTGTATCTGTTAAGATTGAAGATCCACAGTTTGAAGGACAGACCAAGCAGAA
GCTTGGTAATACAGTTGCAAGGGGAGCAGTTGACAGTATAGTAAGTGAACAGCTTACTTACTTCCTTGAGCAAAATCCTG
CAGTTGCAAAATCTATATGTGAAAAATCGTTGCTTGCACAGCGTGCGAGAGAAGCTGCAAGAAAAGCAAGAGACTTAACA
AGAAGAAAGACTGCACTTGAAAGCACTTCACTTCCTGGTAAGTTAGCTGACTGTTCAGATAAAGACCCTAAGAACTGTGA
GATATATATTGTTGAGGGAGATTCAGCTGGTGGTTCAGCTAAGACTGCAAGAAGCCGTGCAACACAGGCTATCCTTCCAC
TTCGTGGTAAAATTCTTAACGTTGAGAAAGCAAGACTTGACAGAATACTTGGTAATGCAGAGATTAAAGCGATGATTACT
GCATTTGGTACAGGCATTCATGAAGATTTTGATATAAGCAAGCTTCGTTATCATAAGATTATTATTATGACAGATGCCGA
TGTAGATGGTGCACATATTGCTACACTTCTACTTACATTCCTGTACAGATTCATGCCTGATCTTATAAGAGAAGGCCATG
TATATCTTGCACAGCCACCACTTTATAAAGTTGAGAAGAATAAAAAAGTGTGGTATGCCTATAATGATGATGAGCTTAAT
GCAATCATGACTGAGATTGGAAGAGACCAGAATAATAAAGTCCAGCGTTATAAGGGACTTGGTGAGATGGACGCAGAGCA
GTTATGGGAAACAACAATGGACCCACACAAGAGAGTATTATTAAGAGTTAATATGAATGAAGATGATGATTCAGAACTTG
ATGTAACATTCTCAACACTTATGGGTGACAAGGTAGAGCCAAGAAGAGATTTCATAGAAGCAAATGCTAAGTTTGTAAAG
AATTTGGATATCTAA

Upstream 100 bases:

>100_bases
ATAACAGAATGAATATCAACAAAATATTTAAAGTAACAGATGGTCATGTAGTTAATGAGAATTAATTACATTTTGCTTTG
TAAAACAAGGAGGAATTAAA

Downstream 100 bases:

>100_bases
CAAGCCATGCACAAGGAAAAATTATAAATATGACCGTATGCTTGCATACATGGGCATATTTTATAATTTTGAGTTGTATG
TGGCGATTAGCCACATAAGT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 644; Mature: 643

Protein sequence:

>644_residues
MADQNAEKNYGGDQIQVLEGLEAVRKRPGMYIGSTSGRGLHHLVYEIVDNAIDEALAGFCTHIEVMINKDNSITVIDNGR
GVPVDINHKTGRPAIEVVYTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSEWLEVQVKRNGHIYQQRYERGKICYDLKI
VGDCDIEDTGTQVTFLPDSEIFQETTVFEFDILMHRLREMAFLTKGIKITLTDLREGLEQQKVFHYEGGIKEFVQYLNKS
KEPLYSQIIYCEGVKDNVQVEVAFQHNDGYNEVVDSFVNNIKTPEGGTHLTGFRNSLTKTFNDYARKNKILKDSDQGLSG
DDIREGLTAIVSVKIEDPQFEGQTKQKLGNTVARGAVDSIVSEQLTYFLEQNPAVAKSICEKSLLAQRAREAARKARDLT
RRKTALESTSLPGKLADCSDKDPKNCEIYIVEGDSAGGSAKTARSRATQAILPLRGKILNVEKARLDRILGNAEIKAMIT
AFGTGIHEDFDISKLRYHKIIIMTDADVDGAHIATLLLTFLYRFMPDLIREGHVYLAQPPLYKVEKNKKVWYAYNDDELN
AIMTEIGRDQNNKVQRYKGLGEMDAEQLWETTMDPHKRVLLRVNMNEDDDSELDVTFSTLMGDKVEPRRDFIEANAKFVK
NLDI

Sequences:

>Translated_644_residues
MADQNAEKNYGGDQIQVLEGLEAVRKRPGMYIGSTSGRGLHHLVYEIVDNAIDEALAGFCTHIEVMINKDNSITVIDNGR
GVPVDINHKTGRPAIEVVYTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSEWLEVQVKRNGHIYQQRYERGKICYDLKI
VGDCDIEDTGTQVTFLPDSEIFQETTVFEFDILMHRLREMAFLTKGIKITLTDLREGLEQQKVFHYEGGIKEFVQYLNKS
KEPLYSQIIYCEGVKDNVQVEVAFQHNDGYNEVVDSFVNNIKTPEGGTHLTGFRNSLTKTFNDYARKNKILKDSDQGLSG
DDIREGLTAIVSVKIEDPQFEGQTKQKLGNTVARGAVDSIVSEQLTYFLEQNPAVAKSICEKSLLAQRAREAARKARDLT
RRKTALESTSLPGKLADCSDKDPKNCEIYIVEGDSAGGSAKTARSRATQAILPLRGKILNVEKARLDRILGNAEIKAMIT
AFGTGIHEDFDISKLRYHKIIIMTDADVDGAHIATLLLTFLYRFMPDLIREGHVYLAQPPLYKVEKNKKVWYAYNDDELN
AIMTEIGRDQNNKVQRYKGLGEMDAEQLWETTMDPHKRVLLRVNMNEDDDSELDVTFSTLMGDKVEPRRDFIEANAKFVK
NLDI
>Mature_643_residues
ADQNAEKNYGGDQIQVLEGLEAVRKRPGMYIGSTSGRGLHHLVYEIVDNAIDEALAGFCTHIEVMINKDNSITVIDNGRG
VPVDINHKTGRPAIEVVYTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSEWLEVQVKRNGHIYQQRYERGKICYDLKIV
GDCDIEDTGTQVTFLPDSEIFQETTVFEFDILMHRLREMAFLTKGIKITLTDLREGLEQQKVFHYEGGIKEFVQYLNKSK
EPLYSQIIYCEGVKDNVQVEVAFQHNDGYNEVVDSFVNNIKTPEGGTHLTGFRNSLTKTFNDYARKNKILKDSDQGLSGD
DIREGLTAIVSVKIEDPQFEGQTKQKLGNTVARGAVDSIVSEQLTYFLEQNPAVAKSICEKSLLAQRAREAARKARDLTR
RKTALESTSLPGKLADCSDKDPKNCEIYIVEGDSAGGSAKTARSRATQAILPLRGKILNVEKARLDRILGNAEIKAMITA
FGTGIHEDFDISKLRYHKIIIMTDADVDGAHIATLLLTFLYRFMPDLIREGHVYLAQPPLYKVEKNKKVWYAYNDDELNA
IMTEIGRDQNNKVQRYKGLGEMDAEQLWETTMDPHKRVLLRVNMNEDDDSELDVTFSTLMGDKVEPRRDFIEANAKFVKN
LDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=664, Percent_Identity=25.7530120481928, Blast_Score=156, Evalue=6e-38,
Organism=Homo sapiens, GI19913406, Length=686, Percent_Identity=26.0932944606414, Blast_Score=152, Evalue=1e-36,
Organism=Escherichia coli, GI48994957, Length=563, Percent_Identity=54.8845470692718, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1789408, Length=630, Percent_Identity=40.3174603174603, Blast_Score=456, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17535065, Length=660, Percent_Identity=27.8787878787879, Blast_Score=184, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI212645845, Length=596, Percent_Identity=26.1744966442953, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI212645657, Length=253, Percent_Identity=28.0632411067194, Blast_Score=91, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324241, Length=665, Percent_Identity=26.9172932330827, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI17136538, Length=599, Percent_Identity=26.2103505843072, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72144; Mature: 72013

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQNAEKNYGGDQIQVLEGLEAVRKRPGMYIGSTSGRGLHHLVYEIVDNAIDEALAGFC
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
THIEVMINKDNSITVIDNGRGVPVDINHKTGRPAIEVVYTVLHAGGKFGGGGYKVSGGLH
HEEEEEEECCCEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCC
GVGASVVNALSEWLEVQVKRNGHIYQQRYERGKICYDLKIVGDCDIEDTGTQVTFLPDSE
CHHHHHHHHHHHHHHEEHHCCCCHHHHHHHCCCEEEEEEEEECCCCCCCCCEEEECCCHH
IFQETTVFEFDILMHRLREMAFLTKGIKITLTDLREGLEQQKVFHYEGGIKEFVQYLNKS
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCHHHHHEEHHCCCHHHHHHHHCCC
KEPLYSQIIYCEGVKDNVQVEVAFQHNDGYNEVVDSFVNNIKTPEGGTHLTGFRNSLTKT
CCCHHHHEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
FNDYARKNKILKDSDQGLSGDDIREGLTAIVSVKIEDPQFEGQTKQKLGNTVARGAVDSI
HHHHHHHCCCCCCCCCCCCCHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
VSEQLTYFLEQNPAVAKSICEKSLLAQRAREAARKARDLTRRKTALESTSLPGKLADCSD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
KDPKNCEIYIVEGDSAGGSAKTARSRATQAILPLRGKILNVEKARLDRILGNAEIKAMIT
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHHHHCCCHHHHHHH
AFGTGIHEDFDISKLRYHKIIIMTDADVDGAHIATLLLTFLYRFMPDLIREGHVYLAQPP
HHCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
LYKVEKNKKVWYAYNDDELNAIMTEIGRDQNNKVQRYKGLGEMDAEQLWETTMDPHKRVL
CEEEECCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHCCCCCCEEE
LRVNMNEDDDSELDVTFSTLMGDKVEPRRDFIEANAKFVKNLDI
EEEECCCCCCCCEEEEHHHHCCCCCCHHHHHHHHCHHHHHCCCC
>Mature Secondary Structure 
ADQNAEKNYGGDQIQVLEGLEAVRKRPGMYIGSTSGRGLHHLVYEIVDNAIDEALAGFC
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
THIEVMINKDNSITVIDNGRGVPVDINHKTGRPAIEVVYTVLHAGGKFGGGGYKVSGGLH
HEEEEEEECCCEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCC
GVGASVVNALSEWLEVQVKRNGHIYQQRYERGKICYDLKIVGDCDIEDTGTQVTFLPDSE
CHHHHHHHHHHHHHHEEHHCCCCHHHHHHHCCCEEEEEEEEECCCCCCCCCEEEECCCHH
IFQETTVFEFDILMHRLREMAFLTKGIKITLTDLREGLEQQKVFHYEGGIKEFVQYLNKS
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCHHHHHEEHHCCCHHHHHHHHCCC
KEPLYSQIIYCEGVKDNVQVEVAFQHNDGYNEVVDSFVNNIKTPEGGTHLTGFRNSLTKT
CCCHHHHEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
FNDYARKNKILKDSDQGLSGDDIREGLTAIVSVKIEDPQFEGQTKQKLGNTVARGAVDSI
HHHHHHHCCCCCCCCCCCCCHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
VSEQLTYFLEQNPAVAKSICEKSLLAQRAREAARKARDLTRRKTALESTSLPGKLADCSD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
KDPKNCEIYIVEGDSAGGSAKTARSRATQAILPLRGKILNVEKARLDRILGNAEIKAMIT
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHHHHCCCHHHHHHH
AFGTGIHEDFDISKLRYHKIIIMTDADVDGAHIATLLLTFLYRFMPDLIREGHVYLAQPP
HHCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
LYKVEKNKKVWYAYNDDELNAIMTEIGRDQNNKVQRYKGLGEMDAEQLWETTMDPHKRVL
CEEEECCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHCCCCCCEEE
LRVNMNEDDDSELDVTFSTLMGDKVEPRRDFIEANAKFVKNLDI
EEEECCCCCCCCEEEEHHHHCCCCCCHHHHHHHHCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]