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The map label for this gene is recF [H]

Identifier: 238897210

GI number: 238897210

Start: 5210356

End: 5211429

Strand: Reverse

Name: recF [H]

Synonym: KP1_5481

Alternate gene names: 238897210

Gene position: 5211429-5210356 (Counterclockwise)

Preceding gene: 238897211

Following gene: 238897209

Centisome position: 99.29

GC content: 57.45

Gene sequence:

>1074_bases
ATGTCGCTATCCCGACTTCTAATCAAAGATTTCCGCAATATAGAACATGCGGATCTCGCTTTATCTCCCGGCTTTAACTT
CCTGGTTGGCGCGAACGGCAGCGGCAAAACCAGCGTGCTGGAAGCTATCTACACGCTTGGCCACGGCCGGGCATTTCGCA
GTTTGCAAATTGGCCGAGTGATCCGCCACGAGCAGGATGCGTTTGTTCTGCATGGCCGTCTGCAGGGCGAAGAGCGGGAA
ACCGCCATCGGCCTGACCAAAGACAAACAGGGCGACAGCAAGGTGCGTATCGACGGCACCGACGGCCACAAAGTGGCGGA
GCTGGCGCACCTGATGCCGATGCAGCTGATTACGCCGGAGGGGTTTACTTTACTCAACGGCGGCCCCAAATACAGAAGAG
CATTCCTTGACTGGGGATGCTTTCATAACGAAGCCGGATTCTTCACCGCCTGGAGCAATCTGAAGCGCCTGGTCAAGCAG
CGCAACGCCGCGCTGCGTCAGGTCAGCCGCTATGCCCAGCTGCGGCCTTGGGATCTGGAATTAATCCCGCTGGCGGAGCA
GATCAGCCGCTGGCGTGCCGAATACAGCGCCGCTATCGTCGAAGACATGGCGGATACCTGTCAGCAATTTTTACCGGAAT
TCACGCTCACCTTCTCTTTCCAGCGCGGCTGGGAAAAAGAGACCGACTACGCGGAGGTGTTGGAGCGGAATTTCGAGCGC
GACAGAATGTTAACCTACACCGCCCACGGCCCGCATAAAGCGGATTTTCGCATTCGCGCCGACGGGGCGCCGGTGGAAGA
TACCTTGTCGCGCGGGCAGCTTAAGCTGCTGATGTGCGCCCTGCGTCTGGCGCAAGGAGAGTTCCTCACCCGCGTCAGCG
GGCGGCGCTGCCTGTACCTGATAGATGATTTTGCCTCGGAACTTGATGATGCCCGCCGCGGTCTGTTATCCAGCCGCCTG
AAAGCGACGCAGTCGCAGGTTTTCGTCAGCGCGATCAGCGCTGAACACGTTATGGACATGTCGGACAAAAATTCGAAGAT
GTTCCGCGTGGAAAAAGGTAAAATAACGGATTAA

Upstream 100 bases:

>100_bases
AGCAAATGCTCACGTACTTCGTGTACGCTCCGCTTTCTGCGCGCTGGCGGTAGACGAACTGGCTGCGACACCCACGCCCC
TTGAATGATGACGAGCTGAT

Downstream 100 bases:

>100_bases
CCCAAGATTAAATGAGCGAGAAACGTTGATGTCGAATTCTTATGACTCCTCCAGTATCAAAGTCCTGAAAGGGCTGGATG
CGGTGCGTAAGCGCCCGGGT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSLSRLLIKDFRNIEHADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRVIRHEQDAFVLHGRLQGEERE
TAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEAGFFTAWSNLKRLVKQ
RNAALRQVSRYAQLRPWDLELIPLAEQISRWRAEYSAAIVEDMADTCQQFLPEFTLTFSFQRGWEKETDYAEVLERNFER
DRMLTYTAHGPHKADFRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRVSGRRCLYLIDDFASELDDARRGLLSSRL
KATQSQVFVSAISAEHVMDMSDKNSKMFRVEKGKITD

Sequences:

>Translated_357_residues
MSLSRLLIKDFRNIEHADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRVIRHEQDAFVLHGRLQGEERE
TAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEAGFFTAWSNLKRLVKQ
RNAALRQVSRYAQLRPWDLELIPLAEQISRWRAEYSAAIVEDMADTCQQFLPEFTLTFSFQRGWEKETDYAEVLERNFER
DRMLTYTAHGPHKADFRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRVSGRRCLYLIDDFASELDDARRGLLSSRL
KATQSQVFVSAISAEHVMDMSDKNSKMFRVEKGKITD
>Mature_356_residues
SLSRLLIKDFRNIEHADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRVIRHEQDAFVLHGRLQGEERET
AIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEAGFFTAWSNLKRLVKQR
NAALRQVSRYAQLRPWDLELIPLAEQISRWRAEYSAAIVEDMADTCQQFLPEFTLTFSFQRGWEKETDYAEVLERNFERD
RMLTYTAHGPHKADFRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRVSGRRCLYLIDDFASELDDARRGLLSSRLK
ATQSQVFVSAISAEHVMDMSDKNSKMFRVEKGKITD

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=357, Percent_Identity=93.2773109243697, Blast_Score=692, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40560; Mature: 40429

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSRLLIKDFRNIEHADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRV
CCHHHHHHHHHCCCCCCCEEECCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
IRHEQDAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPE
HHCCCCEEEEEEECCCCCHHHEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHEECCC
GFTLLNGGPKYRRAFLDWGCFHNEAGFFTAWSNLKRLVKQRNAALRQVSRYAQLRPWDLE
CCEEECCCHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LIPLAEQISRWRAEYSAAIVEDMADTCQQFLPEFTLTFSFQRGWEKETDYAEVLERNFER
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEHHCCCCCCCCHHHHHHCCCCC
DRMLTYTAHGPHKADFRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRVSGRRCLYL
CCEEEEECCCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCCEEEEE
IDDFASELDDARRGLLSSRLKATQSQVFVSAISAEHVMDMSDKNSKMFRVEKGKITD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
SLSRLLIKDFRNIEHADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRV
CHHHHHHHHHCCCCCCCEEECCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
IRHEQDAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPE
HHCCCCEEEEEEECCCCCHHHEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHEECCC
GFTLLNGGPKYRRAFLDWGCFHNEAGFFTAWSNLKRLVKQRNAALRQVSRYAQLRPWDLE
CCEEECCCHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LIPLAEQISRWRAEYSAAIVEDMADTCQQFLPEFTLTFSFQRGWEKETDYAEVLERNFER
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEHHCCCCCCCCHHHHHHCCCCC
DRMLTYTAHGPHKADFRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRVSGRRCLYL
CCEEEEECCCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCCEEEEE
IDDFASELDDARRGLLSSRLKATQSQVFVSAISAEHVMDMSDKNSKMFRVEKGKITD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA