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The map label for this gene is nepI [H]

Identifier: 238897171

GI number: 238897171

Start: 5170986

End: 5172179

Strand: Reverse

Name: nepI [H]

Synonym: KP1_5438

Alternate gene names: 238897171

Gene position: 5172179-5170986 (Counterclockwise)

Preceding gene: 238897174

Following gene: 238897168

Centisome position: 98.55

GC content: 60.22

Gene sequence:

>1194_bases
ATGAGTCATTCAAATGAAACCAAACCCCATGCACGCGATCTCGCCCGGCCAAACTGGTCGGCGGTCTTCGCGGTTGCTTT
CTGCGTTGCCTGCCTGATTACCGTTGAGTTTTTGCCGGTCAGCCTGCTGACGCCGATGGCGCTGGATTTAGGCATCTCCG
AAGGGATGGCCGGGCAGTCGGTGACGACGACGGCATTCGTCGCGATGTTTTCCAGCCTGTTTATCACCACGGTGATAGGT
AAAACCGATCGCCGCTATGTGGTTATTCTGTTTTCGCTGCTGCTGACCTTTTCCTGCCTGCTGGTATCTTTCGCTGATAG
CTTCACCCTGCTACTACTGGGGCGCGCCTGTCTGGGCCTGGCGCTCGGCGGTTTCTGGGCAATGTCGGCCTCGCTGACCA
TGCGGCTGGTGCCGATGCGCGTGGTGCCGAAAGCGCTATCGATTATCTTCGGCGCGGTATCAATCGCGCTGGTGATCGCC
GCCCCGTTGGGCAGTTTCCTTGGCGGGCTTATCGGCTGGCGCAACGTCTTTAACGGCGCGGCGGTAATGGGCGTGCTGTG
TACGCTCTGGGTGCTGAAAGCGCTGCCGTCGCTGCCCGGGGAATCGGCTTCTCAGCAGCAAAATATGTTTGGTCTGCTGA
AGCGTCCGGGGGTGATGGCCGGAATGTGCGCCATTTTCATGGCCTTTGCCGGACAGTTCGCCTTCTTTACCTATATTCGC
CCGGTTTACATGACCCTCGCCGGGTTTGATGTCGACGGCCTGACGCTGGTGCTGCTGAGCTTTGGTATCGCCAGCTTTAT
CGGCACCTCGCTCTCCTCGGTGCTGCTCAAGCGTTCGGTAAAAGCGGCGCTGGCGATCGCGCCGCTGGTGCTGACCGCCT
GCGCGGTGGCGCTGGTGCTGTGGGGAGAGAGCAAAATCATCGCCTCGACGGTGGCGATTATCTGGGGCTTCGCGTTTGCG
CTGATCCCGGTGGGGTGGTCAACGTGGATCACCCGTTCGCTCTCCGATCAGGCGGAAAAAGCCGGGTCGATTCAGGTGGC
GGTGATCCAGCTGGCCAACACCTGCGGCGCCGCGGTCGGCGGCATTGCGCTTGACCACTTAGGGCTGCTGTCGCCGCTGG
TGTTGTCAGGGATATTGATGCTGTTTACCGGCCTGCTGGTGGCGGCCAAAGTGAAGGTGAATTCACCCGCTTAA

Upstream 100 bases:

>100_bases
TCCTCTTATGAAAAGCGAGAAGAGTTGCGAAAACGTTAAGTACGTCACATACAAAATGTTACACTGCGCAGCTTTCCGCC
ACTTTCTTCGCAGGTCACTC

Downstream 100 bases:

>100_bases
GGCCGCTCGCCTTTTGCCAGCCGGGCATTGATGTCGGCAATCACTTCCGGCAGGTCCGCCAGGGTATCAACCACATAATG
CGCCCCGGCGGCATACAGTT

Product: ribonucleoside transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSHSNETKPHARDLARPNWSAVFAVAFCVACLITVEFLPVSLLTPMALDLGISEGMAGQSVTTTAFVAMFSSLFITTVIG
KTDRRYVVILFSLLLTFSCLLVSFADSFTLLLLGRACLGLALGGFWAMSASLTMRLVPMRVVPKALSIIFGAVSIALVIA
APLGSFLGGLIGWRNVFNGAAVMGVLCTLWVLKALPSLPGESASQQQNMFGLLKRPGVMAGMCAIFMAFAGQFAFFTYIR
PVYMTLAGFDVDGLTLVLLSFGIASFIGTSLSSVLLKRSVKAALAIAPLVLTACAVALVLWGESKIIASTVAIIWGFAFA
LIPVGWSTWITRSLSDQAEKAGSIQVAVIQLANTCGAAVGGIALDHLGLLSPLVLSGILMLFTGLLVAAKVKVNSPA

Sequences:

>Translated_397_residues
MSHSNETKPHARDLARPNWSAVFAVAFCVACLITVEFLPVSLLTPMALDLGISEGMAGQSVTTTAFVAMFSSLFITTVIG
KTDRRYVVILFSLLLTFSCLLVSFADSFTLLLLGRACLGLALGGFWAMSASLTMRLVPMRVVPKALSIIFGAVSIALVIA
APLGSFLGGLIGWRNVFNGAAVMGVLCTLWVLKALPSLPGESASQQQNMFGLLKRPGVMAGMCAIFMAFAGQFAFFTYIR
PVYMTLAGFDVDGLTLVLLSFGIASFIGTSLSSVLLKRSVKAALAIAPLVLTACAVALVLWGESKIIASTVAIIWGFAFA
LIPVGWSTWITRSLSDQAEKAGSIQVAVIQLANTCGAAVGGIALDHLGLLSPLVLSGILMLFTGLLVAAKVKVNSPA
>Mature_396_residues
SHSNETKPHARDLARPNWSAVFAVAFCVACLITVEFLPVSLLTPMALDLGISEGMAGQSVTTTAFVAMFSSLFITTVIGK
TDRRYVVILFSLLLTFSCLLVSFADSFTLLLLGRACLGLALGGFWAMSASLTMRLVPMRVVPKALSIIFGAVSIALVIAA
PLGSFLGGLIGWRNVFNGAAVMGVLCTLWVLKALPSLPGESASQQQNMFGLLKRPGVMAGMCAIFMAFAGQFAFFTYIRP
VYMTLAGFDVDGLTLVLLSFGIASFIGTSLSSVLLKRSVKAALAIAPLVLTACAVALVLWGESKIIASTVAIIWGFAFAL
IPVGWSTWITRSLSDQAEKAGSIQVAVIQLANTCGAAVGGIALDHLGLLSPLVLSGILMLFTGLLVAAKVKVNSPA

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. NepI (TC 2.A.1.2.26) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=395, Percent_Identity=79.2405063291139, Blast_Score=613, Evalue=1e-177,
Organism=Escherichia coli, GI1787808, Length=246, Percent_Identity=28.0487804878049, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787947, Length=291, Percent_Identity=25.4295532646048, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786595, Length=243, Percent_Identity=23.0452674897119, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41665; Mature: 41534

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SHSNETKPHARDLARPNWSAVFAVAFCVACLITVEFLPVSLLTPMALDLGISEGMAGQS
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA