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The map label for this gene is pcaK [H]

Identifier: 238897157

GI number: 238897157

Start: 5157528

End: 5158898

Strand: Reverse

Name: pcaK [H]

Synonym: KP1_5421

Alternate gene names: 238897157

Gene position: 5158898-5157528 (Counterclockwise)

Preceding gene: 238897158

Following gene: 238897156

Centisome position: 98.29

GC content: 60.54

Gene sequence:

>1371_bases
ATGAATCACACGAACGTTGTCGATGTGAAAGCGTGGATAGATACCCGCCCGGTCTCGCGCTTTCAATGGAACGTGCTGCT
GCTCTGTTTCATCATCATTATGCTGGATGGGTATGACGCCGCGGTGATGGGCTTTGTCGCCCCGGCGCTCATCGAGGACT
GGGGCATCAGCCGGGCCGAAATGGGGCCGATTCTTGGGGCGGCCATGTTCGGCGTCGCCATTGGCGCCCTGGTAGCGGGT
CCGCTTTCTGACCGCTACGGCCGAAAGCGAATTTTACTCTGGTCGGTGGCGCTGTTTGCGCTGTTCAGCCTGGCCGGCGC
CGTGGCGCAAAGCCCGACGCAGCTGGCGCTGATGCGTTTTCTCACCGGCCTTGGCCTTGGCGCGGTGATGCCCAATTGCG
TCACGCTGGTGGCGGAATATATGCCGGAGCGGCGTAAAGGGGTGATGATCACCCTGATGTACAGCGGTTTTAACGTGGGT
TCCGGCCTCGGCGGCTTTATCGCCGCCGGTTTGTTATCGCATTACAGCTGGCACTCGGCGCTGGTTTTTGGCGGCGTGCT
GCCGCTCGTGGTGCTCCCCTTTATGATCGTGATGTTGCCAGAGTCGGCGATGAACATGGTGGCGCGCCGACTACCGGGCG
AGCAGATTGCCAGGGCGCTTAACCGGCTTGGGGGCCAGTTTACGGCTGAAACCGTTTTCCAGCTGAATGCGCCGCCCATC
ACGCGGAGCAGCAAAGTGGCGCAGCTGTTCCGGCATGGCTATGCCCGCGGCACGATAGCGCTGTGGTTAACCTATTTTAT
GGGGCTGTTCGTGATTTACCTGCTGAACGGCTGGCTGCCAACCATTCTCCGCTCCGGCGGGCTGTCGCTGCAGCAGGCGG
CGATGATGACCGGCTTGTTCCAGCTCGGCGGCCCGCTGGGCGGGATCCTTGTCGGTATGCTGATGGATCGCGCTTCGGCG
AAAGCCGTCATTGCGGCAACCTATTTCCTCGGCTGTCTGTGCCTATTGTCCCAGGGGGTGATGGATTTCGGTTCGGCGGC
GCTGTCGGTGCTGATTTTTATCAGCGGGATGTGCATTAACGGCGCGCAGAACGGTCTGCAGGCCTATTCGCCCGCCTATT
ATCAAACCGAGATCCGCGCTACCGGAGTCAGCTGGATGCATGGTATCGGCCGTACCGGGGCTATCCTCAGCTCCACGCTG
GGCGGGATGCTGATGCTGGCAGTGCCGGGACACTCGTCGATCTTTTTGGTGCTTGCGCTGCCGGCCTGCCTGGCGGGGAT
CTGTATTTTGTTGCACCGTATGAATCACGCTAAGCCTCGACTCACAGAGGCAGAACTTGACGCTTTGTCATCCCCCCTCG
AACACCGATAA

Upstream 100 bases:

>100_bases
ACCGGACAGCCTGCGTGACCTGCCTTAAGTTGAGTACGAAAGTTCCACTCAAAAAACACTAACCAGAACATTCGTACCGC
CTGCTTGAGGAAGTCACCCT

Downstream 100 bases:

>100_bases
GTGGAGTTATCTGATGGCTAACATTCTCGGCGGTATCGCCGTCTCTCATACCCCGACTATCGGCTTCGCGGTCGATCATC
ATAAACAGCAGGACCCGGCG

Product: putative general substrate transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MNHTNVVDVKAWIDTRPVSRFQWNVLLLCFIIIMLDGYDAAVMGFVAPALIEDWGISRAEMGPILGAAMFGVAIGALVAG
PLSDRYGRKRILLWSVALFALFSLAGAVAQSPTQLALMRFLTGLGLGAVMPNCVTLVAEYMPERRKGVMITLMYSGFNVG
SGLGGFIAAGLLSHYSWHSALVFGGVLPLVVLPFMIVMLPESAMNMVARRLPGEQIARALNRLGGQFTAETVFQLNAPPI
TRSSKVAQLFRHGYARGTIALWLTYFMGLFVIYLLNGWLPTILRSGGLSLQQAAMMTGLFQLGGPLGGILVGMLMDRASA
KAVIAATYFLGCLCLLSQGVMDFGSAALSVLIFISGMCINGAQNGLQAYSPAYYQTEIRATGVSWMHGIGRTGAILSSTL
GGMLMLAVPGHSSIFLVLALPACLAGICILLHRMNHAKPRLTEAELDALSSPLEHR

Sequences:

>Translated_456_residues
MNHTNVVDVKAWIDTRPVSRFQWNVLLLCFIIIMLDGYDAAVMGFVAPALIEDWGISRAEMGPILGAAMFGVAIGALVAG
PLSDRYGRKRILLWSVALFALFSLAGAVAQSPTQLALMRFLTGLGLGAVMPNCVTLVAEYMPERRKGVMITLMYSGFNVG
SGLGGFIAAGLLSHYSWHSALVFGGVLPLVVLPFMIVMLPESAMNMVARRLPGEQIARALNRLGGQFTAETVFQLNAPPI
TRSSKVAQLFRHGYARGTIALWLTYFMGLFVIYLLNGWLPTILRSGGLSLQQAAMMTGLFQLGGPLGGILVGMLMDRASA
KAVIAATYFLGCLCLLSQGVMDFGSAALSVLIFISGMCINGAQNGLQAYSPAYYQTEIRATGVSWMHGIGRTGAILSSTL
GGMLMLAVPGHSSIFLVLALPACLAGICILLHRMNHAKPRLTEAELDALSSPLEHR
>Mature_456_residues
MNHTNVVDVKAWIDTRPVSRFQWNVLLLCFIIIMLDGYDAAVMGFVAPALIEDWGISRAEMGPILGAAMFGVAIGALVAG
PLSDRYGRKRILLWSVALFALFSLAGAVAQSPTQLALMRFLTGLGLGAVMPNCVTLVAEYMPERRKGVMITLMYSGFNVG
SGLGGFIAAGLLSHYSWHSALVFGGVLPLVVLPFMIVMLPESAMNMVARRLPGEQIARALNRLGGQFTAETVFQLNAPPI
TRSSKVAQLFRHGYARGTIALWLTYFMGLFVIYLLNGWLPTILRSGGLSLQQAAMMTGLFQLGGPLGGILVGMLMDRASA
KAVIAATYFLGCLCLLSQGVMDFGSAALSVLIFISGMCINGAQNGLQAYSPAYYQTEIRATGVSWMHGIGRTGAILSSTL
GGMLMLAVPGHSSIFLVLALPACLAGICILLHRMNHAKPRLTEAELDALSSPLEHR

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=434, Percent_Identity=25.3456221198157, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI7662270, Length=195, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI256219543, Length=288, Percent_Identity=24.3055555555556, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI256219598, Length=288, Percent_Identity=24.3055555555556, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI4503847, Length=288, Percent_Identity=24.3055555555556, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI256219483, Length=288, Percent_Identity=24.3055555555556, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87081723, Length=418, Percent_Identity=34.2105263157895, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87082159, Length=404, Percent_Identity=26.980198019802, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788068, Length=458, Percent_Identity=22.4890829694323, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082231, Length=419, Percent_Identity=23.1503579952267, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87082404, Length=377, Percent_Identity=22.8116710875332, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789152, Length=313, Percent_Identity=22.6837060702875, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786229, Length=366, Percent_Identity=22.1311475409836, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788074, Length=384, Percent_Identity=22.9166666666667, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32564663, Length=407, Percent_Identity=26.2899262899263, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922874, Length=452, Percent_Identity=25.4424778761062, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24640198, Length=222, Percent_Identity=24.7747747747748, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640196, Length=222, Percent_Identity=24.7747747747748, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640200, Length=222, Percent_Identity=24.7747747747748, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48804; Mature: 48804

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHTNVVDVKAWIDTRPVSRFQWNVLLLCFIIIMLDGYDAAVMGFVAPALIEDWGISRAE
CCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHH
MGPILGAAMFGVAIGALVAGPLSDRYGRKRILLWSVALFALFSLAGAVAQSPTQLALMRF
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LTGLGLGAVMPNCVTLVAEYMPERRKGVMITLMYSGFNVGSGLGGFIAAGLLSHYSWHSA
HHHCCHHHHHHHHHHHHHHHCCHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LVFGGVLPLVVLPFMIVMLPESAMNMVARRLPGEQIARALNRLGGQFTAETVFQLNAPPI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCEEHHHHEEECCCCC
TRSSKVAQLFRHGYARGTIALWLTYFMGLFVIYLLNGWLPTILRSGGLSLQQAAMMTGLF
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QLGGPLGGILVGMLMDRASAKAVIAATYFLGCLCLLSQGVMDFGSAALSVLIFISGMCIN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAQNGLQAYSPAYYQTEIRATGVSWMHGIGRTGAILSSTLGGMLMLAVPGHSSIFLVLAL
CHHHHHHHCCCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHCCEEEEEECCCCHHHHHHHH
PACLAGICILLHRMNHAKPRLTEAELDALSSPLEHR
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MNHTNVVDVKAWIDTRPVSRFQWNVLLLCFIIIMLDGYDAAVMGFVAPALIEDWGISRAE
CCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHH
MGPILGAAMFGVAIGALVAGPLSDRYGRKRILLWSVALFALFSLAGAVAQSPTQLALMRF
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LTGLGLGAVMPNCVTLVAEYMPERRKGVMITLMYSGFNVGSGLGGFIAAGLLSHYSWHSA
HHHCCHHHHHHHHHHHHHHHCCHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LVFGGVLPLVVLPFMIVMLPESAMNMVARRLPGEQIARALNRLGGQFTAETVFQLNAPPI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCEEHHHHEEECCCCC
TRSSKVAQLFRHGYARGTIALWLTYFMGLFVIYLLNGWLPTILRSGGLSLQQAAMMTGLF
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QLGGPLGGILVGMLMDRASAKAVIAATYFLGCLCLLSQGVMDFGSAALSVLIFISGMCIN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAQNGLQAYSPAYYQTEIRATGVSWMHGIGRTGAILSSTLGGMLMLAVPGHSSIFLVLAL
CHHHHHHHCCCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHCCEEEEEECCCCHHHHHHHH
PACLAGICILLHRMNHAKPRLTEAELDALSSPLEHR
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]