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The map label for this gene is ligB [H]

Identifier: 238897156

GI number: 238897156

Start: 5156252

End: 5157514

Strand: Reverse

Name: ligB [H]

Synonym: KP1_5420

Alternate gene names: 238897156

Gene position: 5157514-5156252 (Counterclockwise)

Preceding gene: 238897157

Following gene: 238897154

Centisome position: 98.27

GC content: 58.35

Gene sequence:

>1263_bases
ATGGCTAACATTCTCGGCGGTATCGCCGTCTCTCATACCCCGACTATCGGCTTCGCGGTCGATCATCATAAACAGCAGGA
CCCGGCGTGGGCGCCGATCTTCCAGAGCTTCGAACCGCTGCAGCGCTGGCTGGAGGAGAAAAAACCGGATGCGCTGGTGT
ACATCTTTAATGACCATGTGACCGCCTTTTTCTTCGATCACTATTCGACTTTTACGCTGGGAATCGATAGTCAATATGAC
GTTGCCGATGAGGGCGGCGGACCGCGCTGCCTGCCGCCGGTCCAGGGCAACGCGGCGCTCTCAAGGCACATTGGCGCCAG
CCTGATGGCCGACGAGTTTGATATGTCGTTCTTTATGGATAAAAAGCTCGACCACGGCCTTTTCTCGCCGCTTTCGGCAC
TGTTGCCCTGGGATGAAGCGCAGGGGTGGCCGACGGCGGTGATCCCGTTACAGATCGGCGTGCTGCAATTTCCAGTACCA
AGCGCGCGCCGTTGTTACAAACTCGGCCAGGCGCTGCGCCGGGCGATTGAAAGCTTCCCGGAAGATATCAATGTGGCGAT
TGTCGCGACCGGCGGGCTCTCGCATCAGGTTCATGGCGAGCGCTGCGGATTTAATAATCCCGACTGGGATGCCCAGTTTG
TTGATATGCTGGTTAATGACCCGGAAAAGCTTACCGAAATGACCCTTGGCGAATATGCCGAGCTGGGGGGGATGGAGGGG
TCCGAAGTGATCATGTGGCTGGTGATGCGCGGCGCGCTATCAGCCAATGTCACCGAAACCTGGCGCGATTATTACCTGCC
GTCGATGACCGGTATCGCCACGCTGATCCTTGAGAACAATGCGCGCCTGCCCCCCGTCGATACCCTGACGCGCCATCGCC
AGCATATGGCGCAGCAGCTGGCCGGGGTGGAGAAACTGCCGGGAACCTATCCTTTTACCCACGAGCGCAGCCTGAATGGC
TTGCGGCTTAACCGTTTCCTGCATCGGCTGATTGAGCCTGCCTGGCGTGAACGTTTTCTGCAGTCGCCTCAGTCGCTTTA
TGCCGAAGCCGGACTGAGTGAGGAGGAACAGCAGTTGCTCAACGCGCGCGACTGGCGGGGATTAATTCAGTATGGCGCCA
GCTTCTTCCTGCTGGAGAAAATGGGCGCGGTGGTCGGCGTATCCAACCTGCATATTTACGCCGCGATGCGCGGGCAGACG
CTGGAGGCGTTTCAGCAAACGCGCAACCAGCAGGTAACCTATTCGGTGGCGGGTAAACGTTAA

Upstream 100 bases:

>100_bases
ATTTTGTTGCACCGTATGAATCACGCTAAGCCTCGACTCACAGAGGCAGAACTTGACGCTTTGTCATCCCCCCTCGAACA
CCGATAAGTGGAGTTATCTG

Downstream 100 bases:

>100_bases
ACGGCCCCGTCCTGAACCTGCTGATAAATAAAACGCAACAGCGCCTGCGTCGCCGGGGAGGGCAGGCTGCCGGCGCGAAA
CGTCAGCCCGATACGCCGGG

Product: protocatechuate 4,5-dioxygenase

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MANILGGIAVSHTPTIGFAVDHHKQQDPAWAPIFQSFEPLQRWLEEKKPDALVYIFNDHVTAFFFDHYSTFTLGIDSQYD
VADEGGGPRCLPPVQGNAALSRHIGASLMADEFDMSFFMDKKLDHGLFSPLSALLPWDEAQGWPTAVIPLQIGVLQFPVP
SARRCYKLGQALRRAIESFPEDINVAIVATGGLSHQVHGERCGFNNPDWDAQFVDMLVNDPEKLTEMTLGEYAELGGMEG
SEVIMWLVMRGALSANVTETWRDYYLPSMTGIATLILENNARLPPVDTLTRHRQHMAQQLAGVEKLPGTYPFTHERSLNG
LRLNRFLHRLIEPAWRERFLQSPQSLYAEAGLSEEEQQLLNARDWRGLIQYGASFFLLEKMGAVVGVSNLHIYAAMRGQT
LEAFQQTRNQQVTYSVAGKR

Sequences:

>Translated_420_residues
MANILGGIAVSHTPTIGFAVDHHKQQDPAWAPIFQSFEPLQRWLEEKKPDALVYIFNDHVTAFFFDHYSTFTLGIDSQYD
VADEGGGPRCLPPVQGNAALSRHIGASLMADEFDMSFFMDKKLDHGLFSPLSALLPWDEAQGWPTAVIPLQIGVLQFPVP
SARRCYKLGQALRRAIESFPEDINVAIVATGGLSHQVHGERCGFNNPDWDAQFVDMLVNDPEKLTEMTLGEYAELGGMEG
SEVIMWLVMRGALSANVTETWRDYYLPSMTGIATLILENNARLPPVDTLTRHRQHMAQQLAGVEKLPGTYPFTHERSLNG
LRLNRFLHRLIEPAWRERFLQSPQSLYAEAGLSEEEQQLLNARDWRGLIQYGASFFLLEKMGAVVGVSNLHIYAAMRGQT
LEAFQQTRNQQVTYSVAGKR
>Mature_419_residues
ANILGGIAVSHTPTIGFAVDHHKQQDPAWAPIFQSFEPLQRWLEEKKPDALVYIFNDHVTAFFFDHYSTFTLGIDSQYDV
ADEGGGPRCLPPVQGNAALSRHIGASLMADEFDMSFFMDKKLDHGLFSPLSALLPWDEAQGWPTAVIPLQIGVLQFPVPS
ARRCYKLGQALRRAIESFPEDINVAIVATGGLSHQVHGERCGFNNPDWDAQFVDMLVNDPEKLTEMTLGEYAELGGMEGS
EVIMWLVMRGALSANVTETWRDYYLPSMTGIATLILENNARLPPVDTLTRHRQHMAQQLAGVEKLPGTYPFTHERSLNGL
RLNRFLHRLIEPAWRERFLQSPQSLYAEAGLSEEEQQLLNARDWRGLIQYGASFFLLEKMGAVVGVSNLHIYAAMRGQTL
EAFQQTRNQQVTYSVAGKR

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 47147; Mature: 47016

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANILGGIAVSHTPTIGFAVDHHKQQDPAWAPIFQSFEPLQRWLEEKKPDALVYIFNDHV
CCCCCCCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCE
TAFFFDHYSTFTLGIDSQYDVADEGGGPRCLPPVQGNAALSRHIGASLMADEFDMSFFMD
EEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHH
KKLDHGLFSPLSALLPWDEAQGWPTAVIPLQIGVLQFPVPSARRCYKLGQALRRAIESFP
HHHCCHHHHHHHHHCCCHHCCCCCEEEEEEEECEEECCCCHHHHHHHHHHHHHHHHHHCC
EDINVAIVATGGLSHQVHGERCGFNNPDWDAQFVDMLVNDPEKLTEMTLGEYAELGGMEG
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
SEVIMWLVMRGALSANVTETWRDYYLPSMTGIATLILENNARLPPVDTLTRHRQHMAQQL
HHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHH
AGVEKLPGTYPFTHERSLNGLRLNRFLHRLIEPAWRERFLQSPQSLYAEAGLSEEEQQLL
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHCCCCHHHHHHH
NARDWRGLIQYGASFFLLEKMGAVVGVSNLHIYAAMRGQTLEAFQQTRNQQVTYSVAGKR
HHHHHHHHHHHCHHHHHHHHHHHHHEECCEEEEEEECCCHHHHHHHHCCCEEEEEECCCC
>Mature Secondary Structure 
ANILGGIAVSHTPTIGFAVDHHKQQDPAWAPIFQSFEPLQRWLEEKKPDALVYIFNDHV
CCCCCCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCE
TAFFFDHYSTFTLGIDSQYDVADEGGGPRCLPPVQGNAALSRHIGASLMADEFDMSFFMD
EEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHH
KKLDHGLFSPLSALLPWDEAQGWPTAVIPLQIGVLQFPVPSARRCYKLGQALRRAIESFP
HHHCCHHHHHHHHHCCCHHCCCCCEEEEEEEECEEECCCCHHHHHHHHHHHHHHHHHHCC
EDINVAIVATGGLSHQVHGERCGFNNPDWDAQFVDMLVNDPEKLTEMTLGEYAELGGMEG
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
SEVIMWLVMRGALSANVTETWRDYYLPSMTGIATLILENNARLPPVDTLTRHRQHMAQQL
HHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHH
AGVEKLPGTYPFTHERSLNGLRLNRFLHRLIEPAWRERFLQSPQSLYAEAGLSEEEQQLL
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHCCCCHHHHHHH
NARDWRGLIQYGASFFLLEKMGAVVGVSNLHIYAAMRGQTLEAFQQTRNQQVTYSVAGKR
HHHHHHHHHHHCHHHHHHHHHHHHHEECCEEEEEEECCCHHHHHHHHCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]