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The map label for this gene is glyS [H]

Identifier: 238897022

GI number: 238897022

Start: 5013801

End: 5015870

Strand: Reverse

Name: glyS [H]

Synonym: KP1_5269

Alternate gene names: 238897022

Gene position: 5015870-5013801 (Counterclockwise)

Preceding gene: 238897023

Following gene: 238897021

Centisome position: 95.57

GC content: 59.18

Gene sequence:

>2070_bases
ATGTCTGAGAATACTTTTCTGGTGGAAATCGGCACTGAAGAGCTGCCACCAAAAGCGCTGCGCAGCCTGGCGGAATCTTT
TGCTGCGAATGTGACCGCGGAGCTGGATAACGCTGGCCTTGCGCACGGTAAAGTGGAATGGTTTGCCGCCCCGCGTCGTC
TGGCGCTGAAGGTGGCCAACCTGGCGGCGGCGCAAGCCGATCGCGAAGTGGAAAAACGCGGCCCGGCTATTGCCCAGGCG
TTCGATGCCGAAGGCAAGCCAAGCAAAGCGGCCGAAGGCTGGGCGCGCGGCTGCGGTATCACCGTCGATCAGGCCGAACG
TCTGACCACGGACAAAGGCGAGTGGCTGCTGTATCGCGCCCATGTGAAGGGTGAAAGCACCGAAGCGCTGCTGCCGAACA
TGATTGCCAGCTCGCTGGCGAAGCTACCGATCCCGAAACTGATGCGCTGGGGCGCGTCTGACGTCCACTTCGTGCGTCCG
GTACACACGGTGACTTTGCTGCTGGGTGACAAAGTGATCCCGGCCACTATCCTCGGTATTCCGTCCGATCGCGTGATTCG
CGGCCATCGCTTTATGGGCGAGCCGGAGTTCACCATCGACCATGCCGACCAGTACCCGCAGATCCTGCTGGAGCGCGGTA
AAGTCATCGCCGATTACGAACAGCGTAAAGCCAAAATCAAAGCTGATGCGCAAGAGGCGGCGCGTAAGATAGGCGGTCAA
GCCGATCTGAGCGAAAGCCTGCTGGAAGAGGTCACCTCGCTGGTGGAATGGCCGGTGGTGCTGACGGCGAAGTTCGAAGA
AAAATTCCTCGCGGTGCCGTCTGAAGCGCTGGTTTACACCATGAAGGGCGACCAGAAGTACTTCCCGGTCTACGACAACG
CCGGCAAACTGCTGCCGAACTTTATCTTCGTGGCCAACATCGAGTCCAAAGATCCGCAGCAGATCATCTCCGGTAACGAG
AAGGTGGTCCGTCCGCGTCTGGCCGATGCCGAGTTCTTCTTCAATACCGACCGTAAAAAACGTCTGGAAGATAACCTGCC
GCGTCTGGAAACCGTGCTGTTCCAACAGCAACTTGGGACTCTGCGCGACAAGACCGATCGCATCCAGGCGCTGGCGGGTT
GGATTGCCGAACAGATTGGCGCTGACGTGAACCACGCAACCCGCGCGGGCCTGCTGTCCAAGTGCGACCTGATGACCAAC
ATGGTCTTCGAGTTCACCGACACCCAGGGCGTGATGGGGATGCACTACGCGCGCCATGACGGCGAAGCGGAAGACGTGGC
CGTGGCGCTGAACGAGCAGTATCAGCCGCGATTTGCTGGCGACGCGCTGCCGTCTAATCCGGTGGCCTGCGCGGTAGCGA
TTGCCGATAAGATGGACACCCTGGCGGGTATCTTTGGTATCGGTCAGCATCCGAAAGGCGATAAAGACCCGTTTGCGCTG
CGTCGCGCCGCGCTGGGCGTGCTGCGTATCATCGTTGAGAAGAACCTGGATCTCGATCTGCAGACGCTGACCGAAGAAGC
GGTTCGTCTGTACGGTGAGAAGCTGACCAACGCCAACGTGGTTGATGATGTGATCGACTTTATGCTGGGCCGCTTCCGTG
CCTGGTATCAGGACGAAGGCTACGGCGTCGACACGATCCAGGCGGTACTGGCGCGTCGTCCAACCCGCCCGGCGGATTTC
GATGCGCGTATGAAGGCGGTATCGCACTTCCGTACTCTGGAAGAGTCCTCCGCGTTGGCGGCGGCAAACAAACGTGTCTC
CAACATCCTCGCGAAATCCGATGAGACGCTGAACGACATCGTTCACGCTTCGGTGCTGAAAGAAGCGGCGGAAATTAAGC
TGGCGGGTAATCTGGTGGTGCTGCGCGACAAGCTGCAGCCGTATTTCGCCGCAGGCCGTTATCAGGATGCGCTGATTGAG
CTGGCCGCGCTGCGCGAGCCGGTGGATGAGTTCTTCGAGAACGTGATGGTTAACGCTGAAGACAAAGATGTTCGCATCAA
CCGTCTGACGCTGCTGTCCAAACTGCGTGAACTGTTCCTGCAGGTCGCGGATATTTCTCTGCTGCAGTAA

Upstream 100 bases:

>100_bases
GCGCATTCGCACCCTGACTAAAGCAGTGGCGGAAGCGTACTACGCTTCCCGTGAAGCGCTCGGCTTCCCGATGTGCAACA
AGAATAAATAAGAGGCGGCC

Downstream 100 bases:

>100_bases
GCGTATCGGAAATAATAAAAGACCTGCCTCAGGGCAGGTTTTTTTATACGTGAAATATGAATAGGTGAACCGTTAATAAT
TCTGTCATTGATATCTTCAT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MSENTFLVEIGTEELPPKALRSLAESFAANVTAELDNAGLAHGKVEWFAAPRRLALKVANLAAAQADREVEKRGPAIAQA
FDAEGKPSKAAEGWARGCGITVDQAERLTTDKGEWLLYRAHVKGESTEALLPNMIASSLAKLPIPKLMRWGASDVHFVRP
VHTVTLLLGDKVIPATILGIPSDRVIRGHRFMGEPEFTIDHADQYPQILLERGKVIADYEQRKAKIKADAQEAARKIGGQ
ADLSESLLEEVTSLVEWPVVLTAKFEEKFLAVPSEALVYTMKGDQKYFPVYDNAGKLLPNFIFVANIESKDPQQIISGNE
KVVRPRLADAEFFFNTDRKKRLEDNLPRLETVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDALPSNPVACAVAIADKMDTLAGIFGIGQHPKGDKDPFAL
RRAALGVLRIIVEKNLDLDLQTLTEEAVRLYGEKLTNANVVDDVIDFMLGRFRAWYQDEGYGVDTIQAVLARRPTRPADF
DARMKAVSHFRTLEESSALAAANKRVSNILAKSDETLNDIVHASVLKEAAEIKLAGNLVVLRDKLQPYFAAGRYQDALIE
LAALREPVDEFFENVMVNAEDKDVRINRLTLLSKLRELFLQVADISLLQ

Sequences:

>Translated_689_residues
MSENTFLVEIGTEELPPKALRSLAESFAANVTAELDNAGLAHGKVEWFAAPRRLALKVANLAAAQADREVEKRGPAIAQA
FDAEGKPSKAAEGWARGCGITVDQAERLTTDKGEWLLYRAHVKGESTEALLPNMIASSLAKLPIPKLMRWGASDVHFVRP
VHTVTLLLGDKVIPATILGIPSDRVIRGHRFMGEPEFTIDHADQYPQILLERGKVIADYEQRKAKIKADAQEAARKIGGQ
ADLSESLLEEVTSLVEWPVVLTAKFEEKFLAVPSEALVYTMKGDQKYFPVYDNAGKLLPNFIFVANIESKDPQQIISGNE
KVVRPRLADAEFFFNTDRKKRLEDNLPRLETVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDALPSNPVACAVAIADKMDTLAGIFGIGQHPKGDKDPFAL
RRAALGVLRIIVEKNLDLDLQTLTEEAVRLYGEKLTNANVVDDVIDFMLGRFRAWYQDEGYGVDTIQAVLARRPTRPADF
DARMKAVSHFRTLEESSALAAANKRVSNILAKSDETLNDIVHASVLKEAAEIKLAGNLVVLRDKLQPYFAAGRYQDALIE
LAALREPVDEFFENVMVNAEDKDVRINRLTLLSKLRELFLQVADISLLQ
>Mature_688_residues
SENTFLVEIGTEELPPKALRSLAESFAANVTAELDNAGLAHGKVEWFAAPRRLALKVANLAAAQADREVEKRGPAIAQAF
DAEGKPSKAAEGWARGCGITVDQAERLTTDKGEWLLYRAHVKGESTEALLPNMIASSLAKLPIPKLMRWGASDVHFVRPV
HTVTLLLGDKVIPATILGIPSDRVIRGHRFMGEPEFTIDHADQYPQILLERGKVIADYEQRKAKIKADAQEAARKIGGQA
DLSESLLEEVTSLVEWPVVLTAKFEEKFLAVPSEALVYTMKGDQKYFPVYDNAGKLLPNFIFVANIESKDPQQIISGNEK
VVRPRLADAEFFFNTDRKKRLEDNLPRLETVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLLSKCDLMTNM
VFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDALPSNPVACAVAIADKMDTLAGIFGIGQHPKGDKDPFALR
RAALGVLRIIVEKNLDLDLQTLTEEAVRLYGEKLTNANVVDDVIDFMLGRFRAWYQDEGYGVDTIQAVLARRPTRPADFD
ARMKAVSHFRTLEESSALAAANKRVSNILAKSDETLNDIVHASVLKEAAEIKLAGNLVVLRDKLQPYFAAGRYQDALIEL
AALREPVDEFFENVMVNAEDKDVRINRLTLLSKLRELFLQVADISLLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=91.8722786647315, Blast_Score=1288, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 76332; Mature: 76201

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENTFLVEIGTEELPPKALRSLAESFAANVTAELDNAGLAHGKVEWFAAPRRLALKVAN
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCEECCCHHHHHHHHH
LAAAQADREVEKRGPAIAQAFDAEGKPSKAAEGWARGCGITVDQAERLTTDKGEWLLYRA
HHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEE
HVKGESTEALLPNMIASSLAKLPIPKLMRWGASDVHFVRPVHTVTLLLGDKVIPATILGI
EECCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEECHHHHHHHHHCCCCCCHHEECC
PSDRVIRGHRFMGEPEFTIDHADQYPQILLERGKVIADYEQRKAKIKADAQEAARKIGGQ
CCCCEEECCCCCCCCCCEECCCHHHHHHHHHCCCEEECHHHHHHHHHCCHHHHHHHCCCC
ADLSESLLEEVTSLVEWPVVLTAKFEEKFLAVPSEALVYTMKGDQKYFPVYDNAGKLLPN
CCHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCCCCEEEEEECCCEECCEECCCCHHCCC
FIFVANIESKDPQQIISGNEKVVRPRLADAEFFFNTDRKKRLEDNLPRLETVLFQQQLGT
EEEEEECCCCCHHHHHCCCCCEECCCCCCCHHEECCCHHHHHHCCCHHHHHHHHHHHHHH
LRDKTDRIQALAGWIAEQIGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHCCC
GEAEDVAVALNEQYQPRFAGDALPSNPVACAVAIADKMDTLAGIFGIGQHPKGDKDPFAL
CCHHHEEEEECCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGVLRIIVEKNLDLDLQTLTEEAVRLYGEKLTNANVVDDVIDFMLGRFRAWYQDEG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
YGVDTIQAVLARRPTRPADFDARMKAVSHFRTLEESSALAAANKRVSNILAKSDETLNDI
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VHASVLKEAAEIKLAGNLVVLRDKLQPYFAAGRYQDALIELAALREPVDEFFENVMVNAE
HHHHHHHHHHHEEEECCEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCC
DKDVRINRLTLLSKLRELFLQVADISLLQ
CCCEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SENTFLVEIGTEELPPKALRSLAESFAANVTAELDNAGLAHGKVEWFAAPRRLALKVAN
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCEECCCHHHHHHHHH
LAAAQADREVEKRGPAIAQAFDAEGKPSKAAEGWARGCGITVDQAERLTTDKGEWLLYRA
HHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEE
HVKGESTEALLPNMIASSLAKLPIPKLMRWGASDVHFVRPVHTVTLLLGDKVIPATILGI
EECCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEECHHHHHHHHHCCCCCCHHEECC
PSDRVIRGHRFMGEPEFTIDHADQYPQILLERGKVIADYEQRKAKIKADAQEAARKIGGQ
CCCCEEECCCCCCCCCCEECCCHHHHHHHHHCCCEEECHHHHHHHHHCCHHHHHHHCCCC
ADLSESLLEEVTSLVEWPVVLTAKFEEKFLAVPSEALVYTMKGDQKYFPVYDNAGKLLPN
CCHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCCCCEEEEEECCCEECCEECCCCHHCCC
FIFVANIESKDPQQIISGNEKVVRPRLADAEFFFNTDRKKRLEDNLPRLETVLFQQQLGT
EEEEEECCCCCHHHHHCCCCCEECCCCCCCHHEECCCHHHHHHCCCHHHHHHHHHHHHHH
LRDKTDRIQALAGWIAEQIGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHCCC
GEAEDVAVALNEQYQPRFAGDALPSNPVACAVAIADKMDTLAGIFGIGQHPKGDKDPFAL
CCHHHEEEEECCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGVLRIIVEKNLDLDLQTLTEEAVRLYGEKLTNANVVDDVIDFMLGRFRAWYQDEG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
YGVDTIQAVLARRPTRPADFDARMKAVSHFRTLEESSALAAANKRVSNILAKSDETLNDI
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VHASVLKEAAEIKLAGNLVVLRDKLQPYFAAGRYQDALIELAALREPVDEFFENVMVNAE
HHHHHHHHHHHEEEECCEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCC
DKDVRINRLTLLSKLRELFLQVADISLLQ
CCCEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA