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The map label for this gene is nikB [H]

Identifier: 238896948

GI number: 238896948

Start: 4922434

End: 4923378

Strand: Direct

Name: nikB [H]

Synonym: KP1_5189

Alternate gene names: 238896948

Gene position: 4922434-4923378 (Clockwise)

Preceding gene: 238896947

Following gene: 238896949

Centisome position: 93.79

GC content: 64.02

Gene sequence:

>945_bases
ATGCTACGCTATATTTTACGCCGCCTGCTGCTGCTGATCCCGCTGCTGCTCGCCGCCTCCGCCATCATCTTTCTGCTGCT
GCGGCTCGGCGCCGGCGACCCGGCGCTGGATTATTTACGCCTGTCGAACCTGCCGCCCACTGAAGAGATGGTTGCCTCGA
CCCGCGAGCTGCTGGGGCTCAACCAGCCGCTGGCAATGCAGTATCTGCACTGGCTATGGCGGGCGCTGCACCTGGATTTC
GGTCTCTCCTATGCCACCCAGCGCCCGGTGCTGGACGACCTGCTGCACTTCCTGCCGGCCACCCTGCTGCTCACCGGCGC
GGCGCTGGCGCTGATCCTCGTCACCTCCCTGCCGTTGGGCATCTGGGCGGCGCGCCATCGCGATCGCCTGCCGGACTATA
TCGTACGGCTGATTGCCTTCCTCGGCGTCTCAATGCCCAACTTCTGGCTGGCCTTTATGCTGGTGATGCTCTTTTCCGTG
CATCTGCAGTGGCTCCCGGCGATGGGGTATGGCGACTGGCAGCACCTGATATTGCCCGCCGTCTCCATCGCCTTTATGTC
GCTGGCGATCAACGCCCGCCTGCTGCGCGCCAGCATGCTGGACGCCGCCAGCCAGCGTCACGTGATATGGGCGCGGCTAC
GCGGATTGAGCGCGCGCCAGGTGGAGCGCCGACATATCCTGCGCAATGCCACCCTGCCGGTGGTGACCGCCATGGGCATG
CACATTGGCGAGCTGATCGGCGGCACCATGATTATCGAAAATATCTTTGCCTGGCCCGGGGTCGGCCGCTACGCGGTTTC
CGCCATTTTCAATCGCGATTACCCGGTGATCCAGTGCTTTACCCTGATGATGGTGACGGTGTTCGTGCTGGCTAATCTGG
CGGTGGATGTGCTTAACGCGGCGCTCGACCCGCGCCTGCGCCGCCACGAGGAGGTGAGCGCATGA

Upstream 100 bases:

>100_bases
TCTCGCTGATGTCGGTCGCGCGCCAGGAGGTCGGGGAGATCCCGTTCGCCCCGGTGACCAGCGAGATCCCTTTCGACCAG
ATTACCCCGGTAACGCCATA

Downstream 100 bases:

>100_bases
GAGGGCAACAAAGCTTTCGCTGGCCGGTAAAACTGGCCATGCTGGTTATCGCGCTGCTGGCGGTCATCGCCATCGGCAGC
GGCTGGTGGCTGCCCTGGGA

Product: nickel transporter permease NikB

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MLRYILRRLLLLIPLLLAASAIIFLLLRLGAGDPALDYLRLSNLPPTEEMVASTRELLGLNQPLAMQYLHWLWRALHLDF
GLSYATQRPVLDDLLHFLPATLLLTGAALALILVTSLPLGIWAARHRDRLPDYIVRLIAFLGVSMPNFWLAFMLVMLFSV
HLQWLPAMGYGDWQHLILPAVSIAFMSLAINARLLRASMLDAASQRHVIWARLRGLSARQVERRHILRNATLPVVTAMGM
HIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPVIQCFTLMMVTVFVLANLAVDVLNAALDPRLRRHEEVSA

Sequences:

>Translated_314_residues
MLRYILRRLLLLIPLLLAASAIIFLLLRLGAGDPALDYLRLSNLPPTEEMVASTRELLGLNQPLAMQYLHWLWRALHLDF
GLSYATQRPVLDDLLHFLPATLLLTGAALALILVTSLPLGIWAARHRDRLPDYIVRLIAFLGVSMPNFWLAFMLVMLFSV
HLQWLPAMGYGDWQHLILPAVSIAFMSLAINARLLRASMLDAASQRHVIWARLRGLSARQVERRHILRNATLPVVTAMGM
HIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPVIQCFTLMMVTVFVLANLAVDVLNAALDPRLRRHEEVSA
>Mature_314_residues
MLRYILRRLLLLIPLLLAASAIIFLLLRLGAGDPALDYLRLSNLPPTEEMVASTRELLGLNQPLAMQYLHWLWRALHLDF
GLSYATQRPVLDDLLHFLPATLLLTGAALALILVTSLPLGIWAARHRDRLPDYIVRLIAFLGVSMPNFWLAFMLVMLFSV
HLQWLPAMGYGDWQHLILPAVSIAFMSLAINARLLRASMLDAASQRHVIWARLRGLSARQVERRHILRNATLPVVTAMGM
HIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPVIQCFTLMMVTVFVLANLAVDVLNAALDPRLRRHEEVSA

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=314, Percent_Identity=82.484076433121, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI1787053, Length=308, Percent_Identity=41.8831168831169, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1789965, Length=341, Percent_Identity=34.3108504398827, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1787497, Length=310, Percent_Identity=35.4838709677419, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1787761, Length=331, Percent_Identity=35.9516616314199, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1787550, Length=275, Percent_Identity=30.1818181818182, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788504, Length=360, Percent_Identity=24.7222222222222, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014156 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35320; Mature: 35320

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRYILRRLLLLIPLLLAASAIIFLLLRLGAGDPALDYLRLSNLPPTEEMVASTRELLGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCC
NQPLAMQYLHWLWRALHLDFGLSYATQRPVLDDLLHFLPATLLLTGAALALILVTSLPLG
CCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IWAARHRDRLPDYIVRLIAFLGVSMPNFWLAFMLVMLFSVHLQWLPAMGYGDWQHLILPA
HHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VSIAFMSLAINARLLRASMLDAASQRHVIWARLRGLSARQVERRHILRNATLPVVTAMGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
HIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPVIQCFTLMMVTVFVLANLAVDVLNA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALDPRLRRHEEVSA
HHCHHHHHHHHCCC
>Mature Secondary Structure
MLRYILRRLLLLIPLLLAASAIIFLLLRLGAGDPALDYLRLSNLPPTEEMVASTRELLGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCC
NQPLAMQYLHWLWRALHLDFGLSYATQRPVLDDLLHFLPATLLLTGAALALILVTSLPLG
CCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IWAARHRDRLPDYIVRLIAFLGVSMPNFWLAFMLVMLFSVHLQWLPAMGYGDWQHLILPA
HHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VSIAFMSLAINARLLRASMLDAASQRHVIWARLRGLSARQVERRHILRNATLPVVTAMGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
HIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPVIQCFTLMMVTVFVLANLAVDVLNA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALDPRLRRHEEVSA
HHCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]