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The map label for this gene is nikA [H]

Identifier: 238896947

GI number: 238896947

Start: 4920866

End: 4922434

Strand: Direct

Name: nikA [H]

Synonym: KP1_5188

Alternate gene names: 238896947

Gene position: 4920866-4922434 (Clockwise)

Preceding gene: 238896946

Following gene: 238896948

Centisome position: 93.76

GC content: 63.29

Gene sequence:

>1569_bases
TTGTCTATTATCCGCCTCACCCTGCTGGCGCTGGTCGCCTGCGTCCCTCTGCTGGCTCAGGCCGCGCCGTACCAGCTCAC
CACCGCCTGGCCGGTTAACGTCGGCCCGCTGAATCCCCATCTCTATACGCCCAACCAGATGTTTGCCCAAAGCATGGTCT
ATGAACCGCTGGTGAAGTATCAGGCTGACGGCAGCGTACAGCCCTGGCTGGCAACCCGCTGGCGTCACTCCGCCGACGGC
AAAACCTGGTGGTTCACCCTGCGCGACGATGTGGCCTTCTCCAACGGCGAACCGTTTAACGCCCAGGCCGCGGCGGCCAA
TTTTCAGGCGGTGCTGGCCAACCGCCAACGCCACGCCTGGCTTGAGCTCGCCAACCAAATAACCGACGTGCGGGCCCTCA
GCGCCACCGAGCTGCAGATCACTCTGAAAAGCGCCTACGCCCCCTTACTGCAAGAGCTGGCCCTGCCCCGCCCGTTCCGC
TTCATCGCCCCCTCGCAGTTTATCGATGGCGGCACCGCCAGGGGCATTAAAGCCCCGATCGGCACCGGCCCGTGGCGGCT
GGCAAGCTCGCAGCTCAATCAGCGCGATGTGCTGGTGCGCAATGAGCGCTACTGGGGTCGCAAACCCGCGCTACAGCAAA
TCACCATCAAGGTGATCCCTGACGCCACCAGCCGGGCGGTGGCGTTCGAAACCGGGGAAATCGATATGCTCTACGGCGAT
GAAGGGCTGCTGCCGCTGGACACCTTCGAACGCTTTCGCCATCACCCCGGCTACGTGGCTCGTCTGTCCGCCCCGGCGGA
GACGGTAATGCTGGCGCTAAACGCCAGCCAGGGCCCGACCCGCGAGCTGACGGTTCGCGAAGCGCTCAACTACGCCGTCG
ATAAACAGACCCTTGTCGATAGCGTGTTGTATGGCACCCAGCAGGTTGCCGACACCCTGTTTGCCCCCTCCGTCCCTTAC
GCCCCGCAGGATCTGACGCCGCGCCGTTACGACCCGACAAAAGCCCGCGCCCTGCTGGAACAGGCCGGCTGGCAGCAGCT
GGAAGGCCAGCCCTGGCGACAAAAAGCCGGGCAACCGCTGGCGATTGAGCTGGCGTTTATTGGCACCGACGCGCTGGCTA
AATCGATGGCCGAAATTATTCAGGCCAATCTGCGGCAGGTCGGCGTGCAGGTCACCCTGGTGGGCGAGGAGGAAAGCAGC
ATTTACGCGCGCCAGCGCGAAGGCCGCTTCGGCATGATCTTCAATCGCACCTGGGGCGCGCCCTACGATCCGCATGCCTT
TTTAAGCTCGATGCGCGTCCCGTCGCATGCGGATTACCAGGCCCAGCGCGGCCTGCCAGACAAGGCACTGATTGATAAAG
AGATTAGTGAAGTGCTGACCACCGGGGACGAGGCGCAGCGGAGAAAACTGTACCATGATGTGCTGACCCGTCTGCATCAG
GACGCGGTGTATCTGCCCATCAGCTATGTCTCGCTGATGTCGGTCGCGCGCCAGGAGGTCGGGGAGATCCCGTTCGCCCC
GGTGACCAGCGAGATCCCTTTCGACCAGATTACCCCGGTAACGCCATAA

Upstream 100 bases:

>100_bases
GATTTTCTTATACTTTCCGGCGCAATATTGATCCAGGTTACTCCCAAAGTATGATGAATCACTAAAATCGTCATACTTTC
CTGTCGTTGAGATTGCCGTC

Downstream 100 bases:

>100_bases
TGCTACGCTATATTTTACGCCGCCTGCTGCTGCTGATCCCGCTGCTGCTCGCCGCCTCCGCCATCATCTTTCTGCTGCTG
CGGCTCGGCGCCGGCGACCC

Product: nickel transport system periplasmic binding component

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSIIRLTLLALVACVPLLAQAAPYQLTTAWPVNVGPLNPHLYTPNQMFAQSMVYEPLVKYQADGSVQPWLATRWRHSADG
KTWWFTLRDDVAFSNGEPFNAQAAAANFQAVLANRQRHAWLELANQITDVRALSATELQITLKSAYAPLLQELALPRPFR
FIAPSQFIDGGTARGIKAPIGTGPWRLASSQLNQRDVLVRNERYWGRKPALQQITIKVIPDATSRAVAFETGEIDMLYGD
EGLLPLDTFERFRHHPGYVARLSAPAETVMLALNASQGPTRELTVREALNYAVDKQTLVDSVLYGTQQVADTLFAPSVPY
APQDLTPRRYDPTKARALLEQAGWQQLEGQPWRQKAGQPLAIELAFIGTDALAKSMAEIIQANLRQVGVQVTLVGEEESS
IYARQREGRFGMIFNRTWGAPYDPHAFLSSMRVPSHADYQAQRGLPDKALIDKEISEVLTTGDEAQRRKLYHDVLTRLHQ
DAVYLPISYVSLMSVARQEVGEIPFAPVTSEIPFDQITPVTP

Sequences:

>Translated_522_residues
MSIIRLTLLALVACVPLLAQAAPYQLTTAWPVNVGPLNPHLYTPNQMFAQSMVYEPLVKYQADGSVQPWLATRWRHSADG
KTWWFTLRDDVAFSNGEPFNAQAAAANFQAVLANRQRHAWLELANQITDVRALSATELQITLKSAYAPLLQELALPRPFR
FIAPSQFIDGGTARGIKAPIGTGPWRLASSQLNQRDVLVRNERYWGRKPALQQITIKVIPDATSRAVAFETGEIDMLYGD
EGLLPLDTFERFRHHPGYVARLSAPAETVMLALNASQGPTRELTVREALNYAVDKQTLVDSVLYGTQQVADTLFAPSVPY
APQDLTPRRYDPTKARALLEQAGWQQLEGQPWRQKAGQPLAIELAFIGTDALAKSMAEIIQANLRQVGVQVTLVGEEESS
IYARQREGRFGMIFNRTWGAPYDPHAFLSSMRVPSHADYQAQRGLPDKALIDKEISEVLTTGDEAQRRKLYHDVLTRLHQ
DAVYLPISYVSLMSVARQEVGEIPFAPVTSEIPFDQITPVTP
>Mature_521_residues
SIIRLTLLALVACVPLLAQAAPYQLTTAWPVNVGPLNPHLYTPNQMFAQSMVYEPLVKYQADGSVQPWLATRWRHSADGK
TWWFTLRDDVAFSNGEPFNAQAAAANFQAVLANRQRHAWLELANQITDVRALSATELQITLKSAYAPLLQELALPRPFRF
IAPSQFIDGGTARGIKAPIGTGPWRLASSQLNQRDVLVRNERYWGRKPALQQITIKVIPDATSRAVAFETGEIDMLYGDE
GLLPLDTFERFRHHPGYVARLSAPAETVMLALNASQGPTRELTVREALNYAVDKQTLVDSVLYGTQQVADTLFAPSVPYA
PQDLTPRRYDPTKARALLEQAGWQQLEGQPWRQKAGQPLAIELAFIGTDALAKSMAEIIQANLRQVGVQVTLVGEEESSI
YARQREGRFGMIFNRTWGAPYDPHAFLSSMRVPSHADYQAQRGLPDKALIDKEISEVLTTGDEAQRRKLYHDVLTRLHQD
AVYLPISYVSLMSVARQEVGEIPFAPVTSEIPFDQITPVTP

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=523, Percent_Identity=73.6137667304015, Blast_Score=807, Evalue=0.0,
Organism=Escherichia coli, GI1789966, Length=536, Percent_Identity=25.9328358208955, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787762, Length=527, Percent_Identity=25.426944971537, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1787052, Length=521, Percent_Identity=25.7197696737044, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1787495, Length=530, Percent_Identity=25.6603773584906, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789397, Length=541, Percent_Identity=24.5841035120148, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081878, Length=463, Percent_Identity=24.4060475161987, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1787551, Length=472, Percent_Identity=23.0932203389831, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58179; Mature: 58048

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIRLTLLALVACVPLLAQAAPYQLTTAWPVNVGPLNPHLYTPNQMFAQSMVYEPLVKY
CHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHH
QADGSVQPWLATRWRHSADGKTWWFTLRDDVAFSNGEPFNAQAAAANFQAVLANRQRHAW
CCCCCCCCHHHHHCCCCCCCCEEEEEEECCEEECCCCCCCCHHHHHHHHHHHHCCHHHHH
LELANQITDVRALSATELQITLKSAYAPLLQELALPRPFRFIAPSQFIDGGTARGIKAPI
HHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCCCCCCCCC
GTGPWRLASSQLNQRDVLVRNERYWGRKPALQQITIKVIPDATSRAVAFETGEIDMLYGD
CCCCHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCEEEEECC
EGLLPLDTFERFRHHPGYVARLSAPAETVMLALNASQGPTRELTVREALNYAVDKQTLVD
CCCCCHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
SVLYGTQQVADTLFAPSVPYAPQDLTPRRYDPTKARALLEQAGWQQLEGQPWRQKAGQPL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHCCCCHHHHCCCCE
AIELAFIGTDALAKSMAEIIQANLRQVGVQVTLVGEEESSIYARQREGRFGMIFNRTWGA
EEEEEEECHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHCCCCEEEEEECCCCC
PYDPHAFLSSMRVPSHADYQAQRGLPDKALIDKEISEVLTTGDEAQRRKLYHDVLTRLHQ
CCCHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
DAVYLPISYVSLMSVARQEVGEIPFAPVTSEIPFDQITPVTP
CCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SIIRLTLLALVACVPLLAQAAPYQLTTAWPVNVGPLNPHLYTPNQMFAQSMVYEPLVKY
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHH
QADGSVQPWLATRWRHSADGKTWWFTLRDDVAFSNGEPFNAQAAAANFQAVLANRQRHAW
CCCCCCCCHHHHHCCCCCCCCEEEEEEECCEEECCCCCCCCHHHHHHHHHHHHCCHHHHH
LELANQITDVRALSATELQITLKSAYAPLLQELALPRPFRFIAPSQFIDGGTARGIKAPI
HHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCCCCCCCCC
GTGPWRLASSQLNQRDVLVRNERYWGRKPALQQITIKVIPDATSRAVAFETGEIDMLYGD
CCCCHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCEEEEECC
EGLLPLDTFERFRHHPGYVARLSAPAETVMLALNASQGPTRELTVREALNYAVDKQTLVD
CCCCCHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
SVLYGTQQVADTLFAPSVPYAPQDLTPRRYDPTKARALLEQAGWQQLEGQPWRQKAGQPL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHCCCCHHHHCCCCE
AIELAFIGTDALAKSMAEIIQANLRQVGVQVTLVGEEESSIYARQREGRFGMIFNRTWGA
EEEEEEECHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHCCCCEEEEEECCCCC
PYDPHAFLSSMRVPSHADYQAQRGLPDKALIDKEISEVLTTGDEAQRRKLYHDVLTRLHQ
CCCHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
DAVYLPISYVSLMSVARQEVGEIPFAPVTSEIPFDQITPVTP
CCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]