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The map label for this gene is dam [H]

Identifier: 238896855

GI number: 238896855

Start: 4818772

End: 4819599

Strand: Reverse

Name: dam [H]

Synonym: KP1_5088

Alternate gene names: 238896855

Gene position: 4819599-4818772 (Counterclockwise)

Preceding gene: 238896856

Following gene: 238896854

Centisome position: 91.83

GC content: 53.14

Gene sequence:

>828_bases
ATGAAAAAGAATCGCGCTTTTCTGAAGTGGGCAGGGGGAAAGTTCCCCCTGCTTGACGATATTAAAAAGCATTTGCCGGA
AGGCGAGTGCTTGATCGAGCCCTTTGTGGGCGCCGGGTCGGTATTTCTTAATACCGACTTTTCTCGTTATATCCTCGCGG
ATATCAACAGTGACTTAATCGGCCTGTACAACATCGTCAAACTGCGCACCGACGAATACGTCGCCGCCGCCCGTGAGATG
TTTACCCCGGAAAATAACGTCGCTGAGCGCTACTACCTGTATCGTGATGAATTTAACCAAAGCCAGGATCCGCTGCGTCG
CGCGGTGCTGTTCCTGTATTTGAACCGTCACGGCTACAACGGCCTGTGCCGCTACAATCTGCGCGGCGAGTTCAATGTGC
CGTTTGGCCGTTATAAAAAGCCCTATTTCCCCGAAGCTGAGCTCTATCATTTCGCCGAGAAGGCGCAAAACGCCGAGTTT
TACTGCGAATCTTACGAAGAGTGCATGCAACGTGCGGATAGCCGCACAGTGGTGTATTGCGATCCGCCCTATGCGCCGCT
AACGGCGACGGCGAACTTTACCGCCTACCACACCAACAGTTTCAACCTGGAACAACAGGTACTGCTGGCGCAAAAGGCGG
AATCGCTGATGAAAAAGCGCATCCCGGTGCTGATTTCCAACCACCGCACGCCGCTGACGCAGGAATGGTATAAAAACGCG
GCAGAAACGCATATTGTGAAGGTGCGGCGCAGCATCAGCAGCAACGGTGGTACGCGTAAAAAGGTGGACGAGCTATTAGC
GCTCTACCGTCCGCCTAAGACCAAATAA

Upstream 100 bases:

>100_bases
GGTTCAGGCTGACCTGAAATAATGCCGTGGGGTAGTCTTAAGCGCAGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAA
CGTGTAATTAGTTAGGCAGC

Downstream 100 bases:

>100_bases
ATTTCAAGGAGATGCGGATGAAGCAGTATTTGATTGCCCCTTCGATTCTGTCGGCTGATTTTGCCCGTCTGGGTGAGGAT
ACCGCGAAGGCGCTGGCGGC

Product: DNA adenine methylase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKKNRAFLKWAGGKFPLLDDIKKHLPEGECLIEPFVGAGSVFLNTDFSRYILADINSDLIGLYNIVKLRTDEYVAAAREM
FTPENNVAERYYLYRDEFNQSQDPLRRAVLFLYLNRHGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAEF
YCESYEECMQRADSRTVVYCDPPYAPLTATANFTAYHTNSFNLEQQVLLAQKAESLMKKRIPVLISNHRTPLTQEWYKNA
AETHIVKVRRSISSNGGTRKKVDELLALYRPPKTK

Sequences:

>Translated_275_residues
MKKNRAFLKWAGGKFPLLDDIKKHLPEGECLIEPFVGAGSVFLNTDFSRYILADINSDLIGLYNIVKLRTDEYVAAAREM
FTPENNVAERYYLYRDEFNQSQDPLRRAVLFLYLNRHGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAEF
YCESYEECMQRADSRTVVYCDPPYAPLTATANFTAYHTNSFNLEQQVLLAQKAESLMKKRIPVLISNHRTPLTQEWYKNA
AETHIVKVRRSISSNGGTRKKVDELLALYRPPKTK
>Mature_275_residues
MKKNRAFLKWAGGKFPLLDDIKKHLPEGECLIEPFVGAGSVFLNTDFSRYILADINSDLIGLYNIVKLRTDEYVAAAREM
FTPENNVAERYYLYRDEFNQSQDPLRRAVLFLYLNRHGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAEF
YCESYEECMQRADSRTVVYCDPPYAPLTATANFTAYHTNSFNLEQQVLLAQKAESLMKKRIPVLISNHRTPLTQEWYKNA
AETHIVKVRRSISSNGGTRKKVDELLALYRPPKTK

Specific function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplica

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=271, Percent_Identity=82.6568265682657, Blast_Score=473, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32001; Mature: 32001

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNRAFLKWAGGKFPLLDDIKKHLPEGECLIEPFVGAGSVFLNTDFSRYILADINSDLI
CCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCEEEECCCCCHH
GLYNIVKLRTDEYVAAAREMFTPENNVAERYYLYRDEFNQSQDPLRRAVLFLYLNRHGYN
HHHHHHHHCCHHHHHHHHHHCCCCCCCHHHEEEEHHHCCCCHHHHHHHHHHHHEECCCCC
GLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAEFYCESYEECMQRADSRTVVYC
CEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEE
DPPYAPLTATANFTAYHTNSFNLEQQVLLAQKAESLMKKRIPVLISNHRTPLTQEWYKNA
CCCCCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
AETHIVKVRRSISSNGGTRKKVDELLALYRPPKTK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKNRAFLKWAGGKFPLLDDIKKHLPEGECLIEPFVGAGSVFLNTDFSRYILADINSDLI
CCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCEEEECCCCCHH
GLYNIVKLRTDEYVAAAREMFTPENNVAERYYLYRDEFNQSQDPLRRAVLFLYLNRHGYN
HHHHHHHHCCHHHHHHHHHHCCCCCCCHHHEEEEHHHCCCCHHHHHHHHHHHHEECCCCC
GLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAEFYCESYEECMQRADSRTVVYC
CEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEE
DPPYAPLTATANFTAYHTNSFNLEQQVLLAQKAESLMKKRIPVLISNHRTPLTQEWYKNA
CCCCCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
AETHIVKVRRSISSNGGTRKKVDELLALYRPPKTK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]