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The map label for this gene is cysG

Identifier: 238896851

GI number: 238896851

Start: 4814880

End: 4816253

Strand: Direct

Name: cysG

Synonym: KP1_5084

Alternate gene names: 238896851

Gene position: 4814880-4816253 (Clockwise)

Preceding gene: 238896850

Following gene: 238896864

Centisome position: 91.74

GC content: 62.3

Gene sequence:

>1374_bases
GTGGATCACTTACCCATATTCTGCCAGTTGCGCCAGCGCGATTGTTTACTGGTTGGCGGCGGGGATGTCGCCGAACGCAA
AGCCCGTCTGCTGCTGGATGCTGGCGCTAACGTCACGGTCAATGCGCTCGACTTCACGCCCCAGTTTCAGGTGTGGGCTG
ATTCACAGATGCTAACTCTGGTGCAGGGGGAGTTTATCCCCTCGCTGCTGGATAACTGCTGGCTGGCCATCGCCGCTACT
GATGATGAGACGGTCAACCAGCAGGTTAGCGAGGCGGCCGAAGCGCGGCGTATCTTCTGTAACGTGGTGGATGCCCCGCG
CCAGGCCAGCTTTATCATGCCGTCGATCATCGATCGCTCGCCGCTGATGGTCGCCGTCTCCTCGGGCGGGACATCACCGG
TTCTGGCGCGCCTGCTGCGCGAGAAACTGGAGTCAATTCTGCCCCTGCATCTCGGCCAGCTGGCCCGCTATGCCGGCCAT
CTGCGCGCGCGGGTTAAGCAGCAGTTTGCCACCGTAGGCGAGCGTCGCCGCTTCTGGGAGAAGCTGTTTGTTAACGACCG
TCTGGCGCAGTCGCTGGCTAATGACGATCGGCAGGCAGTCGCGGATACCACTGAGCAGTTGCTGACGGAGCCGTTGGAGC
ATCGCGGCGAGGTGGTGCTGGTTGGCGCCGGCCCCGGTGACGCCGGGCTGCTCACGCTCAAAGGCCTGCAGCAGATCCAG
CAGGCTGACGTGGTGGTCTATGATCGGCTGGTCTCCGATGACATCATGAATCTGGTGCGCCGCGATGCCGATCGGGTATT
CGTCGGCAAGCGCTCAGGCTACCACTGCGTCCCGCAGGAGGAGATCAACCAGATCCTGCTGCGCGAAGCGCAAAAAGGAA
GACGCGTGGTCCGGCTGAAAGGCGGCGATCCCTTTATCTTTGGCCGCGGGGGGGAAGAGCTGGAGACGCTTTGCGAGGCG
GGCATCCCCTTCTCCGTTGTGCCTGGCATTACCGCCGCCTCCGGCTGCTCCGCTTATTCGGGGATCCCCTTGACCCATCG
CGATTTCGCGCAGGGCGTCCGCCTGGTCACTGGCCACCTGAAAACCGGCGGCGAGCTGGACTGGGCGAACCTGGCGGTGG
AGAAGCAAACCCTGGTGTTCTACATGGGTCTGAACCAGGCGCCGGCGATCCGCGAGAAGCTTATCGCTCACGGCATGGCT
GAAGATATGCCCGCCGCCATCGTCGAGAACGGCACCGCTGTGACGCAAAAAGTGGTCAGCGGCACCTTAGGACAACTGGA
TATTCTGGCGCAGCAGATGGCCAGCCCGGCGCTGATCATCGTCGGCCGCGTGGTCGGTCTGCGGGATAAACTGAACTGGT
TTTCGAACCATTAA

Upstream 100 bases:

>100_bases
GACAGCGCATGAGTGCGGGATATGTCGCATGTTTTGTATTACCTTTGCTGCTATTCTACGACCACTCGTTTTCTACCCCT
TATTTTTATGAGGTTATTCT

Downstream 100 bases:

>100_bases
CCTTGCCTCCCATAAAGCGACAGGCATAAAAAAGGGCGGGATATCATCCCGCCCTTTTAGCTGTTGCGTCGCTTACGGCT
TGGCGACAAAGCCAATCGCT

Product: siroheme synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MDHLPIFCQLRQRDCLLVGGGDVAERKARLLLDAGANVTVNALDFTPQFQVWADSQMLTLVQGEFIPSLLDNCWLAIAAT
DDETVNQQVSEAAEARRIFCNVVDAPRQASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESILPLHLGQLARYAGH
LRARVKQQFATVGERRRFWEKLFVNDRLAQSLANDDRQAVADTTEQLLTEPLEHRGEVVLVGAGPGDAGLLTLKGLQQIQ
QADVVVYDRLVSDDIMNLVRRDADRVFVGKRSGYHCVPQEEINQILLREAQKGRRVVRLKGGDPFIFGRGGEELETLCEA
GIPFSVVPGITAASGCSAYSGIPLTHRDFAQGVRLVTGHLKTGGELDWANLAVEKQTLVFYMGLNQAPAIREKLIAHGMA
EDMPAAIVENGTAVTQKVVSGTLGQLDILAQQMASPALIIVGRVVGLRDKLNWFSNH

Sequences:

>Translated_457_residues
MDHLPIFCQLRQRDCLLVGGGDVAERKARLLLDAGANVTVNALDFTPQFQVWADSQMLTLVQGEFIPSLLDNCWLAIAAT
DDETVNQQVSEAAEARRIFCNVVDAPRQASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESILPLHLGQLARYAGH
LRARVKQQFATVGERRRFWEKLFVNDRLAQSLANDDRQAVADTTEQLLTEPLEHRGEVVLVGAGPGDAGLLTLKGLQQIQ
QADVVVYDRLVSDDIMNLVRRDADRVFVGKRSGYHCVPQEEINQILLREAQKGRRVVRLKGGDPFIFGRGGEELETLCEA
GIPFSVVPGITAASGCSAYSGIPLTHRDFAQGVRLVTGHLKTGGELDWANLAVEKQTLVFYMGLNQAPAIREKLIAHGMA
EDMPAAIVENGTAVTQKVVSGTLGQLDILAQQMASPALIIVGRVVGLRDKLNWFSNH
>Mature_457_residues
MDHLPIFCQLRQRDCLLVGGGDVAERKARLLLDAGANVTVNALDFTPQFQVWADSQMLTLVQGEFIPSLLDNCWLAIAAT
DDETVNQQVSEAAEARRIFCNVVDAPRQASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESILPLHLGQLARYAGH
LRARVKQQFATVGERRRFWEKLFVNDRLAQSLANDDRQAVADTTEQLLTEPLEHRGEVVLVGAGPGDAGLLTLKGLQQIQ
QADVVVYDRLVSDDIMNLVRRDADRVFVGKRSGYHCVPQEEINQILLREAQKGRRVVRLKGGDPFIFGRGGEELETLCEA
GIPFSVVPGITAASGCSAYSGIPLTHRDFAQGVRLVTGHLKTGGELDWANLAVEKQTLVFYMGLNQAPAIREKLIAHGMA
EDMPAAIVENGTAVTQKVVSGTLGQLDILAQQMASPALIIVGRVVGLRDKLNWFSNH

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789768, Length=457, Percent_Identity=86.4332603938731, Blast_Score=820, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322922, Length=233, Percent_Identity=36.480686695279, Blast_Score=144, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSG2_KLEP7 (A6TF07)

Other databases:

- EMBL:   CP000647
- RefSeq:   YP_001337408.1
- ProteinModelPortal:   A6TF07
- SMR:   A6TF07
- STRING:   A6TF07
- GeneID:   5341883
- GenomeReviews:   CP000647_GR
- KEGG:   kpn:KPN_03754
- eggNOG:   COG0007
- HOGENOM:   HBG730212
- ProtClustDB:   PRK10637
- GO:   GO:0005488
- HAMAP:   MF_01646
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.8.210
- TIGRFAMs:   TIGR01469
- TIGRFAMs:   TIGR01470

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4

Molecular weight: Translated: 49980; Mature: 49980

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00839 SUMT_1; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHLPIFCQLRQRDCLLVGGGDVAERKARLLLDAGANVTVNALDFTPQFQVWADSQMLTL
CCCCCEEEEECCCCEEEECCCCHHHHHHHEEEECCCCEEEEEEECCCCEEEEECCCEEEH
VQGEFIPSLLDNCWLAIAATDDETVNQQVSEAAEARRIFCNVVDAPRQASFIMPSIIDRS
HHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
PLMVAVSSGGTSPVLARLLREKLESILPLHLGQLARYAGHLRARVKQQFATVGERRRFWE
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLFVNDRLAQSLANDDRQAVADTTEQLLTEPLEHRGEVVLVGAGPGDAGLLTLKGLQQIQ
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEHHHHHHHH
QADVVVYDRLVSDDIMNLVRRDADRVFVGKRSGYHCVPQEEINQILLREAQKGRRVVRLK
HCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
GGDPFIFGRGGEELETLCEAGIPFSVVPGITAASGCSAYSGIPLTHRDFAQGVRLVTGHL
CCCEEEECCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHCCHHEEEEEC
KTGGELDWANLAVEKQTLVFYMGLNQAPAIREKLIAHGMAEDMPAAIVENGTAVTQKVVS
CCCCCCCHHHHEEEEEEEEEEECCCCCHHHHHHHHHHCCHHCCCHHHHCCCHHHHHHHHH
GTLGQLDILAQQMASPALIIVGRVVGLRDKLNWFSNH
CCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDHLPIFCQLRQRDCLLVGGGDVAERKARLLLDAGANVTVNALDFTPQFQVWADSQMLTL
CCCCCEEEEECCCCEEEECCCCHHHHHHHEEEECCCCEEEEEEECCCCEEEEECCCEEEH
VQGEFIPSLLDNCWLAIAATDDETVNQQVSEAAEARRIFCNVVDAPRQASFIMPSIIDRS
HHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
PLMVAVSSGGTSPVLARLLREKLESILPLHLGQLARYAGHLRARVKQQFATVGERRRFWE
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLFVNDRLAQSLANDDRQAVADTTEQLLTEPLEHRGEVVLVGAGPGDAGLLTLKGLQQIQ
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEHHHHHHHH
QADVVVYDRLVSDDIMNLVRRDADRVFVGKRSGYHCVPQEEINQILLREAQKGRRVVRLK
HCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
GGDPFIFGRGGEELETLCEAGIPFSVVPGITAASGCSAYSGIPLTHRDFAQGVRLVTGHL
CCCEEEECCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHCCHHEEEEEC
KTGGELDWANLAVEKQTLVFYMGLNQAPAIREKLIAHGMAEDMPAAIVENGTAVTQKVVS
CCCCCCCHHHHEEEEEEEEEEECCCCCHHHHHHHHHHCCHHCCCHHHHCCCHHHHHHHHH
GTLGQLDILAQQMASPALIIVGRVVGLRDKLNWFSNH
CCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA