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The map label for this gene is gltD [H]

Identifier: 238896722

GI number: 238896722

Start: 4702144

End: 4703562

Strand: Direct

Name: gltD [H]

Synonym: KP1_4937

Alternate gene names: 238896722

Gene position: 4702144-4703562 (Clockwise)

Preceding gene: 238896721

Following gene: 238896729

Centisome position: 89.59

GC content: 60.04

Gene sequence:

>1419_bases
ATGAGTCAGAACGTTTATCAATTTATCGACCTGCAACGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGTAAAATTGA
GTTTGTGGAAATTTACGAGCCGTTTTCAGAAGGCCAGGCCAAGGCGCAGGCGGATCGCTGCCTCTCCTGTGGCAACCCGT
ACTGCGAATGGAAGTGCCCGGTGCATAACTACATCCCGAACTGGCTGAAGCTGGCCAACGAGGGGCGTATTTTTGAAGCG
GCCGAGCTGTCGCATCAAACCAACACTCTGCCGGAAGTCTGCGGGCGCGTCTGCCCGCAGGACCGCCTGTGTGAAGGCTC
CTGTACGCTGAACGATGAGTTCGGCGCGGTGACCATCGGCAACATCGAACGCTATATCAACGATAAGGCATTCGAGATGG
GCTGGCGTCCGGATCTCTCCGACGTGAAGCCGACCGGTAAAACCGTCGCGATCATCGGCGCTGGCCCGGCGGGCCTCGCC
TGCGCCGACGTGCTGACCCGTAACGGCGTCAAGGCGGTGGTATTTGACCGCCATCCGGAAATCGGCGGCCTGCTGACCTT
CGGTATCCCGGCGTTCAAGCTGGAAAAAGAGGTGATGACCCGCCGCCGGGAGATCTTCACCGGCATGGGCATTGAGTTCA
AACTCAATACCGAAGTGGGCCGCGACGTGCAGCTCGACGACCTGCTGAAGGAGTACGATGCGGTGTTCCTCGGCGTCGGG
ACCTATCAGTCGATGCGCGGTGGACTGGACAACGAAGACGCTCCGGGTGTTTACGACGCGCTGCCGTTCCTGATCGCTAA
CACTAAGCAGATCATGGGCTTTGGTGAAACCGCCGATGAGCCCTATGTCAGCATGGAAGGCAAACGCGTGGTGGTGCTCG
GCGGTGGCGATACCGCGATGGACTGCGTACGCACCTCCGTCCGTCAGAATGCGGCGCACGTGATCTGCGCCTATCGCCGT
GACGAAGAGAACATGCCCGGCTCAAAACGCGAAGTGAAAAACGCGCGCGAAGAAGGCGTTGAGTTCCAGTTTAACGTCCA
GCCGCTGGGCGTGGAAGTGAATGCCAATGGTAAGGTTTGCGGCGTGAAGATGGCGCGCACCGAAATGGGCCAGCCGGATG
CGAAGGGCCGCCGCCGCGCGGAGATCGTCCCCGGCTCTGAGCACGTGGTGCCGGCTGACGCCGTAGTCATGGCGTTTGGC
TTCCGTCCGCACAGCATGGAGTGGCTGGCGAAGCACAGCGTCGAACTGGACTCCCAGGGCCGTATTATCGCCCCGGAAGG
CAGCGAAAACGCCTTCCAGACCAGCAACCCGAAAATCTTCGCCGGTGGCGATATCGTTCGCGGTTCCGATCTGGTGGTCA
CCGCGATTGCCGAAGGCCGTAAAGCGGCGGACGGGATCCTCAACTACCTTGAAGTCTGA

Upstream 100 bases:

>100_bases
TGCGCTGGTTAAACCAAAGTCCAGCGATGTTAAAGCACTGTTGGGTCACCGTAGTCGTTCCGCAGCTGAGCTGCGGGTGC
AGGCGCAGTAAGGGGTTAAG

Downstream 100 bases:

>100_bases
TTAGGTCCGGAGCCTCACGCATAAAAGCCCGATGGTTAACGTCATCGGGCTTTTTTATTGCTCATTCGCCGGGCAAACCG
CCGCTTTCAGCGCCGTATTA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEA
AELSHQTNTLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDLSDVKPTGKTVAIIGAGPAGLA
CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLDDLLKEYDAVFLGVG
TYQSMRGGLDNEDAPGVYDALPFLIANTKQIMGFGETADEPYVSMEGKRVVVLGGGDTAMDCVRTSVRQNAAHVICAYRR
DEENMPGSKREVKNAREEGVEFQFNVQPLGVEVNANGKVCGVKMARTEMGQPDAKGRRRAEIVPGSEHVVPADAVVMAFG
FRPHSMEWLAKHSVELDSQGRIIAPEGSENAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGILNYLEV

Sequences:

>Translated_472_residues
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEA
AELSHQTNTLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDLSDVKPTGKTVAIIGAGPAGLA
CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLDDLLKEYDAVFLGVG
TYQSMRGGLDNEDAPGVYDALPFLIANTKQIMGFGETADEPYVSMEGKRVVVLGGGDTAMDCVRTSVRQNAAHVICAYRR
DEENMPGSKREVKNAREEGVEFQFNVQPLGVEVNANGKVCGVKMARTEMGQPDAKGRRRAEIVPGSEHVVPADAVVMAFG
FRPHSMEWLAKHSVELDSQGRIIAPEGSENAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGILNYLEV
>Mature_471_residues
SQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAA
ELSHQTNTLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDLSDVKPTGKTVAIIGAGPAGLAC
ADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLDDLLKEYDAVFLGVGT
YQSMRGGLDNEDAPGVYDALPFLIANTKQIMGFGETADEPYVSMEGKRVVVLGGGDTAMDCVRTSVRQNAAHVICAYRRD
EENMPGSKREVKNAREEGVEFQFNVQPLGVEVNANGKVCGVKMARTEMGQPDAKGRRRAEIVPGSEHVVPADAVVMAFGF
RPHSMEWLAKHSVELDSQGRIIAPEGSENAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGILNYLEV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=483, Percent_Identity=28.5714285714286, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1789606, Length=472, Percent_Identity=93.8559322033898, Blast_Score=924, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=461, Percent_Identity=66.8112798264642, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI87082180, Length=450, Percent_Identity=59.1111111111111, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI1788468, Length=442, Percent_Identity=30.7692307692308, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789243, Length=424, Percent_Identity=31.3679245283019, Blast_Score=143, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17570289, Length=492, Percent_Identity=33.130081300813, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI71984108, Length=466, Percent_Identity=30.0429184549356, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17543860, Length=172, Percent_Identity=29.6511627906977, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=481, Percent_Identity=32.2245322245322, Blast_Score=229, Evalue=7e-61,
Organism=Drosophila melanogaster, GI28574881, Length=486, Percent_Identity=38.6831275720165, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24665539, Length=486, Percent_Identity=38.6831275720165, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=38.9690721649485, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=38.9690721649485, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24640763, Length=460, Percent_Identity=27.1739130434783, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI18858217, Length=460, Percent_Identity=27.1739130434783, Blast_Score=148, Evalue=7e-36,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 51911; Mature: 51780

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCP
CCCCCEEHHHHCCCCCCCCCCEEEEEEEEEEECCHHCCHHHHHHHHHHHCCCCCEEEECC
VHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIG
HHHHCCHHHHHCCCCCEEEHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEECCCCCEEEEC
NIERYINDKAFEMGWRPDLSDVKPTGKTVAIIGAGPAGLACADVLTRNGVKAVVFDRHPE
CHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCC
IGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLDDLLKEYDAVFLGVG
CCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCEEEEECC
TYQSMRGGLDNEDAPGVYDALPFLIANTKQIMGFGETADEPYVSMEGKRVVVLGGGDTAM
CHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHEECCCCCCCCEEEECCCEEEEECCCCHHH
DCVRTSVRQNAAHVICAYRRDEENMPGSKREVKNAREEGVEFQFNVQPLGVEVNANGKVC
HHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEEEEEEEECCCCCEE
GVKMARTEMGQPDAKGRRRAEIVPGSEHVVPADAVVMAFGFRPHSMEWLAKHSVELDSQG
EEEEHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCC
RIIAPEGSENAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGILNYLEV
CEECCCCCCCCEECCCCEEEECCCEEECCCEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCP
CCCCEEHHHHCCCCCCCCCCEEEEEEEEEEECCHHCCHHHHHHHHHHHCCCCCEEEECC
VHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIG
HHHHCCHHHHHCCCCCEEEHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEECCCCCEEEEC
NIERYINDKAFEMGWRPDLSDVKPTGKTVAIIGAGPAGLACADVLTRNGVKAVVFDRHPE
CHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCC
IGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLDDLLKEYDAVFLGVG
CCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCEEEEECC
TYQSMRGGLDNEDAPGVYDALPFLIANTKQIMGFGETADEPYVSMEGKRVVVLGGGDTAM
CHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHEECCCCCCCCEEEECCCEEEEECCCCHHH
DCVRTSVRQNAAHVICAYRRDEENMPGSKREVKNAREEGVEFQFNVQPLGVEVNANGKVC
HHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEEEEEEEECCCCCEE
GVKMARTEMGQPDAKGRRRAEIVPGSEHVVPADAVVMAFGFRPHSMEWLAKHSVELDSQG
EEEEHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCC
RIIAPEGSENAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGILNYLEV
CEECCCCCCCCEECCCCEEEECCCEEECCCEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]