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The map label for this gene is dhaM [H]

Identifier: 238896593

GI number: 238896593

Start: 4573426

End: 4574847

Strand: Direct

Name: dhaM [H]

Synonym: KP1_4794

Alternate gene names: 238896593

Gene position: 4573426-4574847 (Clockwise)

Preceding gene: 238896592

Following gene: 238896594

Centisome position: 87.14

GC content: 63.29

Gene sequence:

>1422_bases
ATGGTAAACCTGGTTATTGTTTCTCATAGCGCCCGGCTGGGCGAAGGTGTCGGAGAACTGGCCCGGCAGATGTTAATCAA
CGATGGCTGCAAACTGGCGATCGCCGCCGGGATCGACGATCCCGACAGCCCGATCGGCACCGATCCGCTTAAAGTCATGG
AGGCGATCGAATCCGTGGCCGATACCGATCACGTGCTGGTGATGATGGATATCGGCAGCGCGTTGCTCAGCGCCGAAACC
GCCCTCGATCTGCTCGATCCGGCGATTGCCGCGAAGGTGCGACTGTGCGCCGCGCCGCTGGTGGAAGGGACGCTGGCGGC
CACCGTCAGCGCTGCCTCCGGCGCCGGGATCGACAAGGTGATCGCCGACGCCATGAGCGCCCTGGAGGCCAAGCGGGTTC
AGCTGGGTTTACCTTCGCCAACTTCTGACGCCGCGCCGGCGCCGATGCTTGCCGACGACGGCGACACAAAATCGGTGTCA
GTCATTATTAACAATCATAACGGCCTGCACGTGCGTCCGGCGTCGAAGCTGGTCGCCGCGCTGGCGGGCTTTAACGCCGA
TCTGCTGCTGGAGAAAAACGGGAAGTGCGTCAAACCGGACAGCCTCAACCAAATCGCGCTATTGCAGGTCCGCCGCAACG
ATAAGCTGCGCCTGCTGGCCCGCGGTCCGGATGCCGACGCGGCGCTGGAGGCGTTTCAGGCGCTGGCCGCCGATAACTTC
GGTGAATCGCCTGCGGCGCAACCGGCAGCGGCTCCGGCAACGCCCGAGCGCGTCGAAGGCGCTGCCCTGCGCTATCCGCT
GGCCCTGATCCAGCCGCTCCGCCCGGCTGCCGCCGATGCCGCCCGCGAGCAGCAGCGCCTGCGCCAGGCCATTGACCAGA
CGCTGGCGGATCTTATCGCCCTGACCGAGCTGGCGGAAAATAAATTTAACGCCGATATCGCGGCGATCTTCGCCGGGCAC
CACACCCTGCTCGACGATGACGATCTGTTTGATGCCGCCAACGATCGTCTGCTCACCGAACAATGCACGGCGGAATGGGC
ATGGCATCAGGTGTTGATGGAGCTCAGCCAGCAGTATCGCCAGCTGGATGACCCCTACCTGCAGGCGCGCTATATCGATA
TCGAAGATATTTTGCAGCGCACCCTGCGCCATCTGCAGGGCGCTCAGGAGAGAGTGCCGACGCCCGGCGAGCCAACAATT
ATTATTGCCGACAATATTTATCCCTCAACCGTCTTACAGCTGGATGCCAGCTTCGTTAAAGGCCTGTGCCTGCGCGACGG
CAGCGAACAGGCTCACGGCGCGATTATCGCCCGCGCGGCGGGGATCGCCTGGCTGAGCCAGCAGGGCGAGGCGCTGAACA
GCGTGCAGCCCGGCGAGACTATCGTGCTCGATATGCGCCACCAGCGCCTTATCCGTGACTAA

Upstream 100 bases:

>100_bases
CTCGGCGAGCGCAGCATCGGACATCAGGATCCCGGCGCGACCTCGGTGCTGTTTATGGTTCAGATGCTGGCCGCCGCCGC
CAAAGAGTAAGGAAATCGCG

Downstream 100 bases:

>100_bases
AATTCACGTTGACCGTTACCGCTCCGGGGGCCTGCCTCCGGAGGGTGAGTTTTCGCTTACTCTTCAGGAACCACTATGTC
TCAATTCTTTTTTAACCAGC

Product: dihydroxyacetone kinase subunit M

Products: NA

Alternate protein names: Phosphocarrier protein HPr; Protein H; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MVNLVIVSHSARLGEGVGELARQMLINDGCKLAIAAGIDDPDSPIGTDPLKVMEAIESVADTDHVLVMMDIGSALLSAET
ALDLLDPAIAAKVRLCAAPLVEGTLAATVSAASGAGIDKVIADAMSALEAKRVQLGLPSPTSDAAPAPMLADDGDTKSVS
VIINNHNGLHVRPASKLVAALAGFNADLLLEKNGKCVKPDSLNQIALLQVRRNDKLRLLARGPDADAALEAFQALAADNF
GESPAAQPAAAPATPERVEGAALRYPLALIQPLRPAAADAAREQQRLRQAIDQTLADLIALTELAENKFNADIAAIFAGH
HTLLDDDDLFDAANDRLLTEQCTAEWAWHQVLMELSQQYRQLDDPYLQARYIDIEDILQRTLRHLQGAQERVPTPGEPTI
IIADNIYPSTVLQLDASFVKGLCLRDGSEQAHGAIIARAAGIAWLSQQGEALNSVQPGETIVLDMRHQRLIRD

Sequences:

>Translated_473_residues
MVNLVIVSHSARLGEGVGELARQMLINDGCKLAIAAGIDDPDSPIGTDPLKVMEAIESVADTDHVLVMMDIGSALLSAET
ALDLLDPAIAAKVRLCAAPLVEGTLAATVSAASGAGIDKVIADAMSALEAKRVQLGLPSPTSDAAPAPMLADDGDTKSVS
VIINNHNGLHVRPASKLVAALAGFNADLLLEKNGKCVKPDSLNQIALLQVRRNDKLRLLARGPDADAALEAFQALAADNF
GESPAAQPAAAPATPERVEGAALRYPLALIQPLRPAAADAAREQQRLRQAIDQTLADLIALTELAENKFNADIAAIFAGH
HTLLDDDDLFDAANDRLLTEQCTAEWAWHQVLMELSQQYRQLDDPYLQARYIDIEDILQRTLRHLQGAQERVPTPGEPTI
IIADNIYPSTVLQLDASFVKGLCLRDGSEQAHGAIIARAAGIAWLSQQGEALNSVQPGETIVLDMRHQRLIRD
>Mature_473_residues
MVNLVIVSHSARLGEGVGELARQMLINDGCKLAIAAGIDDPDSPIGTDPLKVMEAIESVADTDHVLVMMDIGSALLSAET
ALDLLDPAIAAKVRLCAAPLVEGTLAATVSAASGAGIDKVIADAMSALEAKRVQLGLPSPTSDAAPAPMLADDGDTKSVS
VIINNHNGLHVRPASKLVAALAGFNADLLLEKNGKCVKPDSLNQIALLQVRRNDKLRLLARGPDADAALEAFQALAADNF
GESPAAQPAAAPATPERVEGAALRYPLALIQPLRPAAADAAREQQRLRQAIDQTLADLIALTELAENKFNADIAAIFAGH
HTLLDDDDLFDAANDRLLTEQCTAEWAWHQVLMELSQQYRQLDDPYLQARYIDIEDILQRTLRHLQGAQERVPTPGEPTI
IIADNIYPSTVLQLDASFVKGLCLRDGSEQAHGAIIARAAGIAWLSQQGEALNSVQPGETIVLDMRHQRLIRD

Specific function: Phosphotransferase subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. DhaM serves as the phosphoryl donor. It is phosphorylated by HPr, then it donates its phosphoryl group to dhaL-ADP, which eventually tran

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI226510935, Length=476, Percent_Identity=65.3361344537815, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI1789193, Length=211, Percent_Identity=29.8578199052133, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI48994992, Length=91, Percent_Identity=35.1648351648352, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788726, Length=312, Percent_Identity=25.9615384615385, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012844
- InterPro:   IPR008279
- InterPro:   IPR004701
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR008731 [H]

Pfam domain/function: PF03610 EIIA-man; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF00381 PTS-HPr [H]

EC number: NA

Molecular weight: Translated: 50386; Mature: 50386

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00369 PTS_HPR_HIS ; PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNLVIVSHSARLGEGVGELARQMLINDGCKLAIAAGIDDPDSPIGTDPLKVMEAIESVA
CEEEEEEECCCHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHC
DTDHVLVMMDIGSALLSAETALDLLDPAIAAKVRLCAAPLVEGTLAATVSAASGAGIDKV
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHH
IADAMSALEAKRVQLGLPSPTSDAAPAPMLADDGDTKSVSVIINNHNGLHVRPASKLVAA
HHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEECHHHHHHHH
LAGFNADLLLEKNGKCVKPDSLNQIALLQVRRNDKLRLLARGPDADAALEAFQALAADNF
HHCCCCEEEEECCCCEECCCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCC
GESPAAQPAAAPATPERVEGAALRYPLALIQPLRPAAADAAREQQRLRQAIDQTLADLIA
CCCCCCCCCCCCCCHHHHCCHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LTELAENKFNADIAAIFAGHHTLLDDDDLFDAANDRLLTEQCTAEWAWHQVLMELSQQYR
HHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QLDDPYLQARYIDIEDILQRTLRHLQGAQERVPTPGEPTIIIADNIYPSTVLQLDASFVK
HCCCCHHEEEECCHHHHHHHHHHHHCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHH
GLCLRDGSEQAHGAIIARAAGIAWLSQQGEALNSVQPGETIVLDMRHQRLIRD
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEECHHHHHCCC
>Mature Secondary Structure
MVNLVIVSHSARLGEGVGELARQMLINDGCKLAIAAGIDDPDSPIGTDPLKVMEAIESVA
CEEEEEEECCCHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHC
DTDHVLVMMDIGSALLSAETALDLLDPAIAAKVRLCAAPLVEGTLAATVSAASGAGIDKV
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHH
IADAMSALEAKRVQLGLPSPTSDAAPAPMLADDGDTKSVSVIINNHNGLHVRPASKLVAA
HHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEECHHHHHHHH
LAGFNADLLLEKNGKCVKPDSLNQIALLQVRRNDKLRLLARGPDADAALEAFQALAADNF
HHCCCCEEEEECCCCEECCCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCC
GESPAAQPAAAPATPERVEGAALRYPLALIQPLRPAAADAAREQQRLRQAIDQTLADLIA
CCCCCCCCCCCCCCHHHHCCHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LTELAENKFNADIAAIFAGHHTLLDDDDLFDAANDRLLTEQCTAEWAWHQVLMELSQQYR
HHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QLDDPYLQARYIDIEDILQRTLRHLQGAQERVPTPGEPTIIIADNIYPSTVLQLDASFVK
HCCCCHHEEEECCHHHHHHHHHHHHCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHH
GLCLRDGSEQAHGAIIARAAGIAWLSQQGEALNSVQPGETIVLDMRHQRLIRD
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEECHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 2671658; 7984428 [H]