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The map label for this gene is dhaK [H]

Identifier: 238896591

GI number: 238896591

Start: 4571702

End: 4572772

Strand: Direct

Name: dhaK [H]

Synonym: KP1_4792

Alternate gene names: 238896591

Gene position: 4571702-4572772 (Clockwise)

Preceding gene: 238896590

Following gene: 238896592

Centisome position: 87.1

GC content: 60.69

Gene sequence:

>1071_bases
ATGAAAAAGCTGATTAACCGTGTTGAAGATGTACTGAATGAACAATTACAGGGGCTGGCTAAAGCCCATCCGCAGCTGAC
CCTGCATCAGGACCCGCTGTATGTGACCCGCACCGACGCGCCGGTGGCTGGCAAGGTGGCCCTGCTCTCCGGCGGCGGCA
GCGGACATGAACCGATGCACTGCGGCTATATCGGCCAGGGCATGCTCTCCGGCGCCTGTCCGGGGGAAATCTTCACCTCG
CCGACGCCGGACAAAATGTTTGAATGCGCGATGCAAATTGACGGCGGCGAAGGCGTCTTGCTGATTATCAAAAACTATAC
CGGCGACATCCTGAATTTTGAGACCGCCACCGAGCTGCTGCATGAGAGCGGCATTAAAGTCACCACCGTGGTGGTCGACG
ACGACGTGGCGGTGAAAGACAGCCTCTATACCGCCGGTCGTCGCGGCGTCGCCAACACCGTACTGATCGAAAAACTGGTC
GGCGCCGCCGCCGAGCGCGGCGACTCGCTGGAAGCCTGCGCTGAACTGGGCCGCCGCCTGAACAATCTCGGCCACTCGAT
TGGTATCGCCCTCGGCGCCTGCACCGTACCGGCTGCCGGGCAGCCCTCCTTTACGCTGAAAGATGACGAGATGGAGTTCG
GCGTCGGCATTCACGGCGAGCCAGGCATCGACCGCCGCCGCTTCAGCTCGCTGGACCAAACCGTCGATGAGATGTTCGAT
ACCCTGCTGGAAAACGGCGCTTACAGCCGCACGCTGCGCCAGTGGGATACCGTCAAGGGCGCGTGGCAGGAAGTGCAGCA
GAGCAAAACCGCGCTGCAAAACGGCGACCGGGTTATCGCGCTGGTTAATAACCTCGGCGCCACTCCGCTTTCCGAACTGT
ATGGCGTGTATAACCGCCTTGCCCAGCGCTGCGAAGCGTCCGGCATCATCATCGAACGCAACCTCATCGGCAGCTACTGT
ACTTCGCTGGATATGGCGGGCTTCTCCATCACTCTGCTGAAAGTGGATGACGAGACGCTGACGCTATGGGACGCCCCGGT
TCACACCCCGGCGCTGAACTGGGGAAACTAA

Upstream 100 bases:

>100_bases
ATCGCCAGGCACGCTCCACCGTCAGACGATACCCCTACGCGATGACCGCCTCCGCCAGCAGAGAGGCGGCATCGAACCTT
CTCCAACGGAGCACTGAATA

Downstream 100 bases:

>100_bases
GGAGCACGACATGTCACTGAACAGAACGCAAATCGTCGACTGGCTGTACCGCTGCGGCGATATTTTCACCAAAGAGAGCG
ATTTTCTCACCGGCCTCGAT

Product: dihydroxyacetone kinase subunit DhaK

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKKLINRVEDVLNEQLQGLAKAHPQLTLHQDPLYVTRTDAPVAGKVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTS
PTPDKMFECAMQIDGGEGVLLIIKNYTGDILNFETATELLHESGIKVTTVVVDDDVAVKDSLYTAGRRGVANTVLIEKLV
GAAAERGDSLEACAELGRRLNNLGHSIGIALGACTVPAAGQPSFTLKDDEMEFGVGIHGEPGIDRRRFSSLDQTVDEMFD
TLLENGAYSRTLRQWDTVKGAWQEVQQSKTALQNGDRVIALVNNLGATPLSELYGVYNRLAQRCEASGIIIERNLIGSYC
TSLDMAGFSITLLKVDDETLTLWDAPVHTPALNWGN

Sequences:

>Translated_356_residues
MKKLINRVEDVLNEQLQGLAKAHPQLTLHQDPLYVTRTDAPVAGKVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTS
PTPDKMFECAMQIDGGEGVLLIIKNYTGDILNFETATELLHESGIKVTTVVVDDDVAVKDSLYTAGRRGVANTVLIEKLV
GAAAERGDSLEACAELGRRLNNLGHSIGIALGACTVPAAGQPSFTLKDDEMEFGVGIHGEPGIDRRRFSSLDQTVDEMFD
TLLENGAYSRTLRQWDTVKGAWQEVQQSKTALQNGDRVIALVNNLGATPLSELYGVYNRLAQRCEASGIIIERNLIGSYC
TSLDMAGFSITLLKVDDETLTLWDAPVHTPALNWGN
>Mature_356_residues
MKKLINRVEDVLNEQLQGLAKAHPQLTLHQDPLYVTRTDAPVAGKVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTS
PTPDKMFECAMQIDGGEGVLLIIKNYTGDILNFETATELLHESGIKVTTVVVDDDVAVKDSLYTAGRRGVANTVLIEKLV
GAAAERGDSLEACAELGRRLNNLGHSIGIALGACTVPAAGQPSFTLKDDEMEFGVGIHGEPGIDRRRFSSLDQTVDEMFD
TLLENGAYSRTLRQWDTVKGAWQEVQQSKTALQNGDRVIALVNNLGATPLSELYGVYNRLAQRCEASGIIIERNLIGSYC
TSLDMAGFSITLLKVDDETLTLWDAPVHTPALNWGN

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=361, Percent_Identity=39.612188365651, Blast_Score=231, Evalue=1e-60,
Organism=Escherichia coli, GI87081857, Length=356, Percent_Identity=87.9213483146067, Blast_Score=655, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17565018, Length=351, Percent_Identity=35.042735042735, Blast_Score=204, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6321055, Length=355, Percent_Identity=34.3661971830986, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6323570, Length=347, Percent_Identity=36.0230547550432, Blast_Score=178, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 38328; Mature: 38328

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLINRVEDVLNEQLQGLAKAHPQLTLHQDPLYVTRTDAPVAGKVALLSGGGSGHEPMH
CHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCEEEEEECCCCCCCCEE
CGYIGQGMLSGACPGEIFTSPTPDKMFECAMQIDGGEGVLLIIKNYTGDILNFETATELL
CCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHEECCCCEEEEEEECCCCCEECHHHHHHHH
HESGIKVTTVVVDDDVAVKDSLYTAGRRGVANTVLIEKLVGAAAERGDSLEACAELGRRL
HHCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
NNLGHSIGIALGACTVPAAGQPSFTLKDDEMEFGVGIHGEPGIDRRRFSSLDQTVDEMFD
HHHCHHHHHHHHHEECCCCCCCCEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHH
TLLENGAYSRTLRQWDTVKGAWQEVQQSKTALQNGDRVIALVNNLGATPLSELYGVYNRL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHH
AQRCEASGIIIERNLIGSYCTSLDMAGFSITLLKVDDETLTLWDAPVHTPALNWGN
HHHHCCCCEEEEHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKKLINRVEDVLNEQLQGLAKAHPQLTLHQDPLYVTRTDAPVAGKVALLSGGGSGHEPMH
CHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCEEEEEECCCCCCCCEE
CGYIGQGMLSGACPGEIFTSPTPDKMFECAMQIDGGEGVLLIIKNYTGDILNFETATELL
CCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHEECCCCEEEEEEECCCCCEECHHHHHHHH
HESGIKVTTVVVDDDVAVKDSLYTAGRRGVANTVLIEKLVGAAAERGDSLEACAELGRRL
HHCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
NNLGHSIGIALGACTVPAAGQPSFTLKDDEMEFGVGIHGEPGIDRRRFSSLDQTVDEMFD
HHHCHHHHHHHHHEECCCCCCCCEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHH
TLLENGAYSRTLRQWDTVKGAWQEVQQSKTALQNGDRVIALVNNLGATPLSELYGVYNRL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHH
AQRCEASGIIIERNLIGSYCTSLDMAGFSITLLKVDDETLTLWDAPVHTPALNWGN
HHHHCCCCEEEEHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]