| Definition | Klebsiella pneumoniae NTUH-K2044 chromosome, complete genome. |
|---|---|
| Accession | NC_012731 |
| Length | 5,248,520 |
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The map label for this gene is dkgA [H]
Identifier: 238896525
GI number: 238896525
Start: 4504145
End: 4504972
Strand: Direct
Name: dkgA [H]
Synonym: KP1_4712
Alternate gene names: 238896525
Gene position: 4504145-4504972 (Clockwise)
Preceding gene: 238896524
Following gene: 238896533
Centisome position: 85.82
GC content: 57.37
Gene sequence:
>828_bases ATGACACATCCAACCGTTATAAAACTGCACGACGGCAACCTGATGCCGCAGCTGGGTCTCGGCGTGTGGAAAGCTGGCAA CGAGGAAGTCGTCTCCGCCATTCATAAGGCGCTGGAAGTCGGTTATCGCTCTTTTGACACCGCCGCCGCGTACCAAAATG AGACCGGCGTCGGCAATGCGCTGCACAGCGCTGGCGTCAACCGCGATGAGCTCTTCATCACCACCAAGCTGTGGAACGAC GATCAAAAACGGCCGCACGAGGCGCTGAAAGAGAGCCTCAGCAAACTGAAGCTGGACTATGTGGATCTCTATTTAATCCA CTGGCCGGTACCGGCTATCGGCCATTACGTCGAGGCCTGGCAGGCGCTGATCGAGCTGCAGCAGCAGGGGCTGACCAAAA GCATTGGCGTGTGTAATTTCCAGGTACCGCATCTGCAGAAGCTGATTGATGAAACCGGCGTCGCCCCGGTGATCAATCAG ATAGAACTCCATCCACTTATGCAGCAGCGTCAGCTGCACGCGTGGAACGCCACCCACAAGATCCAGACCGAATCCTGGAG CCCGCTGGCCCAGGGCGGCGAAGGCGTCTTCGATCAAAAAGTCATCCACCAGCTCGCCGATAAGTACGGCAAAACGCCGG CGCAAATCGTCATCCGCTGGCATCTTGATAGCGGTCTGGTGGTGATCCCGAAATCCGTCACGCCGTCGCGTATCGCGGAA AACTTTGACGTTTGGGACTTCCGTCTCGACAAAGATGAGCTGGGAGCGATCGCGAAGCTGGACCAGGGTAAACGTCTCGG TCCTGACCCGGATCAGTTCGGCGGATAA
Upstream 100 bases:
>100_bases GGGAGAGGTGTTTTTACTACCGTTTTTAGCTATTTCGTCCAGACTTAATGCTACCCCTCGGCGGATCCTGCTTCGCCGAG TTCATTAACAGGAGGAAGGC
Downstream 100 bases:
>100_bases TGCTCTCTCTTGCCATCGCCTTTAGCGCCTCGGCGTTAAAGGCGATGGCAGACCAGATGAATATCCTCTGGCTCCAGCGC AAACAGCGCCGGGTCGGGCT
Product: 2,5-diketo-D-gluconate reductase A
Products: NA
Alternate protein names: 2,5-DKG reductase A; 2,5-DKGR A; 25DKGR-A; AKR5C [H]
Number of amino acids: Translated: 275; Mature: 274
Protein sequence:
>275_residues MTHPTVIKLHDGNLMPQLGLGVWKAGNEEVVSAIHKALEVGYRSFDTAAAYQNETGVGNALHSAGVNRDELFITTKLWND DQKRPHEALKESLSKLKLDYVDLYLIHWPVPAIGHYVEAWQALIELQQQGLTKSIGVCNFQVPHLQKLIDETGVAPVINQ IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGEGVFDQKVIHQLADKYGKTPAQIVIRWHLDSGLVVIPKSVTPSRIAE NFDVWDFRLDKDELGAIAKLDQGKRLGPDPDQFGG
Sequences:
>Translated_275_residues MTHPTVIKLHDGNLMPQLGLGVWKAGNEEVVSAIHKALEVGYRSFDTAAAYQNETGVGNALHSAGVNRDELFITTKLWND DQKRPHEALKESLSKLKLDYVDLYLIHWPVPAIGHYVEAWQALIELQQQGLTKSIGVCNFQVPHLQKLIDETGVAPVINQ IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGEGVFDQKVIHQLADKYGKTPAQIVIRWHLDSGLVVIPKSVTPSRIAE NFDVWDFRLDKDELGAIAKLDQGKRLGPDPDQFGG >Mature_274_residues THPTVIKLHDGNLMPQLGLGVWKAGNEEVVSAIHKALEVGYRSFDTAAAYQNETGVGNALHSAGVNRDELFITTKLWNDD QKRPHEALKESLSKLKLDYVDLYLIHWPVPAIGHYVEAWQALIELQQQGLTKSIGVCNFQVPHLQKLIDETGVAPVINQI ELHPLMQQRQLHAWNATHKIQTESWSPLAQGGEGVFDQKVIHQLADKYGKTPAQIVIRWHLDSGLVVIPKSVTPSRIAEN FDVWDFRLDKDELGAIAKLDQGKRLGPDPDQFGG
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI24497577, Length=295, Percent_Identity=35.5932203389831, Blast_Score=191, Evalue=8e-49, Organism=Homo sapiens, GI5174391, Length=295, Percent_Identity=35.5932203389831, Blast_Score=191, Evalue=8e-49, Organism=Homo sapiens, GI93277124, Length=303, Percent_Identity=34.6534653465347, Blast_Score=188, Evalue=4e-48, Organism=Homo sapiens, GI223468663, Length=295, Percent_Identity=36.6101694915254, Blast_Score=187, Evalue=9e-48, Organism=Homo sapiens, GI310109922, Length=282, Percent_Identity=35.8156028368794, Blast_Score=166, Evalue=2e-41, Organism=Homo sapiens, GI5453543, Length=300, Percent_Identity=34, Blast_Score=164, Evalue=1e-40, Organism=Homo sapiens, GI310109920, Length=300, Percent_Identity=34, Blast_Score=163, Evalue=1e-40, Organism=Homo sapiens, GI45446745, Length=300, Percent_Identity=33.6666666666667, Blast_Score=162, Evalue=2e-40, Organism=Homo sapiens, GI4503285, Length=300, Percent_Identity=33.6666666666667, Blast_Score=162, Evalue=2e-40, Organism=Homo sapiens, GI4502049, Length=292, Percent_Identity=37.3287671232877, Blast_Score=160, Evalue=2e-39, Organism=Homo sapiens, GI291291012, Length=271, Percent_Identity=32.8413284132841, Blast_Score=159, Evalue=2e-39, Organism=Homo sapiens, GI24497583, Length=298, Percent_Identity=32.5503355704698, Blast_Score=153, Evalue=2e-37, Organism=Homo sapiens, GI5174695, Length=299, Percent_Identity=32.4414715719064, Blast_Score=152, Evalue=3e-37, Organism=Homo sapiens, GI24497585, Length=297, Percent_Identity=31.986531986532, Blast_Score=149, Evalue=3e-36, Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=32.8519855595668, Blast_Score=138, Evalue=5e-33, Organism=Homo sapiens, GI300116271, Length=281, Percent_Identity=29.1814946619217, Blast_Score=117, Evalue=1e-26, Organism=Homo sapiens, GI310109926, Length=116, Percent_Identity=42.2413793103448, Blast_Score=89, Evalue=6e-18, Organism=Homo sapiens, GI310109924, Length=152, Percent_Identity=33.5526315789474, Blast_Score=88, Evalue=1e-17, Organism=Homo sapiens, GI207028673, Length=116, Percent_Identity=41.3793103448276, Blast_Score=87, Evalue=1e-17, Organism=Escherichia coli, GI87082198, Length=275, Percent_Identity=85.4545454545455, Blast_Score=494, Evalue=1e-141, Organism=Escherichia coli, GI1786400, Length=256, Percent_Identity=42.1875, Blast_Score=199, Evalue=2e-52, Organism=Escherichia coli, GI1788081, Length=262, Percent_Identity=28.2442748091603, Blast_Score=95, Evalue=4e-21, Organism=Caenorhabditis elegans, GI17537075, Length=298, Percent_Identity=39.5973154362416, Blast_Score=189, Evalue=1e-48, Organism=Caenorhabditis elegans, GI17537077, Length=293, Percent_Identity=38.5665529010239, Blast_Score=186, Evalue=8e-48, Organism=Caenorhabditis elegans, GI17550248, Length=291, Percent_Identity=37.4570446735395, Blast_Score=169, Evalue=2e-42, Organism=Caenorhabditis elegans, GI17561298, Length=274, Percent_Identity=34.6715328467153, Blast_Score=165, Evalue=3e-41, Organism=Caenorhabditis elegans, GI17562292, Length=280, Percent_Identity=34.2857142857143, Blast_Score=160, Evalue=5e-40, Organism=Caenorhabditis elegans, GI17538386, Length=273, Percent_Identity=31.8681318681319, Blast_Score=159, Evalue=1e-39, Organism=Caenorhabditis elegans, GI17564128, Length=300, Percent_Identity=34, Blast_Score=159, Evalue=2e-39, Organism=Caenorhabditis elegans, GI17566692, Length=293, Percent_Identity=32.7645051194539, Blast_Score=155, Evalue=2e-38, Organism=Caenorhabditis elegans, GI17561300, Length=273, Percent_Identity=31.8681318681319, Blast_Score=150, Evalue=7e-37, Organism=Caenorhabditis elegans, GI71998625, Length=269, Percent_Identity=31.9702602230483, Blast_Score=150, Evalue=8e-37, Organism=Caenorhabditis elegans, GI17552492, Length=287, Percent_Identity=30.3135888501742, Blast_Score=150, Evalue=9e-37, Organism=Caenorhabditis elegans, GI17537079, Length=299, Percent_Identity=24.7491638795987, Blast_Score=125, Evalue=3e-29, Organism=Saccharomyces cerevisiae, GI6320576, Length=284, Percent_Identity=36.9718309859155, Blast_Score=202, Evalue=6e-53, Organism=Saccharomyces cerevisiae, GI6324694, Length=287, Percent_Identity=36.5853658536585, Blast_Score=193, Evalue=3e-50, Organism=Saccharomyces cerevisiae, GI6321896, Length=314, Percent_Identity=33.4394904458599, Blast_Score=155, Evalue=9e-39, Organism=Saccharomyces cerevisiae, GI6319625, Length=294, Percent_Identity=32.9931972789116, Blast_Score=149, Evalue=5e-37, Organism=Saccharomyces cerevisiae, GI6322556, Length=262, Percent_Identity=30.9160305343511, Blast_Score=145, Evalue=5e-36, Organism=Saccharomyces cerevisiae, GI6320079, Length=285, Percent_Identity=31.5789473684211, Blast_Score=119, Evalue=6e-28, Organism=Drosophila melanogaster, GI24644950, Length=307, Percent_Identity=36.4820846905537, Blast_Score=199, Evalue=2e-51, Organism=Drosophila melanogaster, GI221378297, Length=327, Percent_Identity=34.2507645259939, Blast_Score=191, Evalue=5e-49, Organism=Drosophila melanogaster, GI20129731, Length=290, Percent_Identity=36.8965517241379, Blast_Score=187, Evalue=6e-48, Organism=Drosophila melanogaster, GI24663317, Length=299, Percent_Identity=34.7826086956522, Blast_Score=181, Evalue=5e-46, Organism=Drosophila melanogaster, GI24657054, Length=293, Percent_Identity=35.8361774744027, Blast_Score=179, Evalue=2e-45, Organism=Drosophila melanogaster, GI24662789, Length=303, Percent_Identity=35.3135313531353, Blast_Score=178, Evalue=3e-45, Organism=Drosophila melanogaster, GI21356425, Length=294, Percent_Identity=33.3333333333333, Blast_Score=174, Evalue=7e-44, Organism=Drosophila melanogaster, GI45553081, Length=294, Percent_Identity=34.3537414965986, Blast_Score=161, Evalue=4e-40, Organism=Drosophila melanogaster, GI24662781, Length=304, Percent_Identity=37.171052631579, Blast_Score=157, Evalue=1e-38, Organism=Drosophila melanogaster, GI24662785, Length=301, Percent_Identity=37.8737541528239, Blast_Score=154, Evalue=6e-38, Organism=Drosophila melanogaster, GI281366140, Length=258, Percent_Identity=32.9457364341085, Blast_Score=136, Evalue=1e-32,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 30744; Mature: 30613
Theoretical pI: Translated: 6.45; Mature: 6.45
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 1.5 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 0.7 %Met (Mature Protein) 1.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTHPTVIKLHDGNLMPQLGLGVWKAGNEEVVSAIHKALEVGYRSFDTAAAYQNETGVGNA CCCCCEEEEECCCCCHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH LHSAGVNRDELFITTKLWNDDQKRPHEALKESLSKLKLDYVDLYLIHWPVPAIGHYVEAW HHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHH QALIELQQQGLTKSIGVCNFQVPHLQKLIDETGVAPVINQIELHPLMQQRQLHAWNATHK HHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCE IQTESWSPLAQGGEGVFDQKVIHQLADKYGKTPAQIVIRWHLDSGLVVIPKSVTPSRIAE ECCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEEECCCEEEEECCCCHHHHHC NFDVWDFRLDKDELGAIAKLDQGKRLGPDPDQFGG CCCCEEEEECHHHHHHHHHHCCCCCCCCCCHHCCC >Mature Secondary Structure THPTVIKLHDGNLMPQLGLGVWKAGNEEVVSAIHKALEVGYRSFDTAAAYQNETGVGNA CCCCEEEEECCCCCHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH LHSAGVNRDELFITTKLWNDDQKRPHEALKESLSKLKLDYVDLYLIHWPVPAIGHYVEAW HHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHH QALIELQQQGLTKSIGVCNFQVPHLQKLIDETGVAPVINQIELHPLMQQRQLHAWNATHK HHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCE IQTESWSPLAQGGEGVFDQKVIHQLADKYGKTPAQIVIRWHLDSGLVVIPKSVTPSRIAE ECCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEEECCCEEEEECCCCHHHHHC NFDVWDFRLDKDELGAIAKLDQGKRLGPDPDQFGG CCCCEEEEECHHHHHHHHHHCCCCCCCCCCHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]