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The map label for this gene is rsmE [H]

Identifier: 238896476

GI number: 238896476

Start: 4458344

End: 4459150

Strand: Direct

Name: rsmE [H]

Synonym: KP1_4654

Alternate gene names: 238896476

Gene position: 4458344-4459150 (Clockwise)

Preceding gene: 238896475

Following gene: 238896477

Centisome position: 84.94

GC content: 58.49

Gene sequence:

>807_bases
TTGTTAATTTGCAGCCCTGTGCTTCCCGCCCGATGGCGGGAAGCTGGCATCTGCGCAACGAACGGAATATCAAGCATGCG
CATTCCTCGCATCCATCATCCAGAACGCCTCATTGTCGGCAGCCAGATCGCGCTGAGCGATGACGCGGCCAACCACGTCG
GCCGCGTCCTGCGCATGACCGCCGGTCAGCACCTACAGCTTTTTGACGGCAGTAACCAGGTGTTTGACGCGGTCATCACC
GAGGCCGGTAAGAAAAACGTTACGGTTGAGGTGCTCAGTGGCGAACCTGACGATCGGGAATCGCCGCTGCATATCCATTT
AGGCCAGGTGATGTCCCGCGGCGAAAAAATGGAATTCACCATCCAGAAATCGATCGAACTCGGGGTAAGCCTCATAACGC
CACTGTTTTCCGAGCGCTGCGGCGTTAAACTGGATGCCGAACGTTTGCAGAAAAAGATCCAGCAGTGGCAGAAAATCGCT
ATTGCCGCCTGCGAACAGAGCGGTCGTAACGTGGTACCGGAAATACGTCCGGCGATGCAGCTGGAGGCGTGGTGCGCCGA
GCAGGACAGCGGGCTGAAGCTCAACCTGCACCCGCGCGCCAGCGCCAGCATTAACACGCTGCCGCTGCCGGTTGAACGCG
TCCGTCTGCTGATTGGCCCGGAAGGCGGGCTGTCGGCTGAAGAGATAGCGATGACCGCGCAATACCAGTTTACTGATATC
CTGTTGGGACCGCGCGTTCTGCGTACAGAGACTACTGCGCTCACCGCCATCACCGCCCTACAGGTGCGTTTTGGCGACTT
AGGTTGA

Upstream 100 bases:

>100_bases
TGTGAACGTGATGAGCGCATCGCGAAAGTGCAGGGCAACCATAATCCGTATGTCCAGCAGGCTTGCCAGGCGCAAAAGAG
CTAACCTACACTAGCGGCAA

Downstream 100 bases:

>100_bases
GGCGTTAACGGAGAAGAACCATGATCAAGCTCGGCATCGTGATGGATCCCATCGCAACCATCAACATCAAGAAAGACACC
AGCTTCGCTATGTTGCTGGA

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MLICSPVLPARWREAGICATNGISSMRIPRIHHPERLIVGSQIALSDDAANHVGRVLRMTAGQHLQLFDGSNQVFDAVIT
EAGKKNVTVEVLSGEPDDRESPLHIHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDAERLQKKIQQWQKIA
IAACEQSGRNVVPEIRPAMQLEAWCAEQDSGLKLNLHPRASASINTLPLPVERVRLLIGPEGGLSAEEIAMTAQYQFTDI
LLGPRVLRTETTALTAITALQVRFGDLG

Sequences:

>Translated_268_residues
MLICSPVLPARWREAGICATNGISSMRIPRIHHPERLIVGSQIALSDDAANHVGRVLRMTAGQHLQLFDGSNQVFDAVIT
EAGKKNVTVEVLSGEPDDRESPLHIHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDAERLQKKIQQWQKIA
IAACEQSGRNVVPEIRPAMQLEAWCAEQDSGLKLNLHPRASASINTLPLPVERVRLLIGPEGGLSAEEIAMTAQYQFTDI
LLGPRVLRTETTALTAITALQVRFGDLG
>Mature_268_residues
MLICSPVLPARWREAGICATNGISSMRIPRIHHPERLIVGSQIALSDDAANHVGRVLRMTAGQHLQLFDGSNQVFDAVIT
EAGKKNVTVEVLSGEPDDRESPLHIHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDAERLQKKIQQWQKIA
IAACEQSGRNVVPEIRPAMQLEAWCAEQDSGLKLNLHPRASASINTLPLPVERVRLLIGPEGGLSAEEIAMTAQYQFTDI
LLGPRVLRTETTALTAITALQVRFGDLG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=87.2427983539095, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 29378; Mature: 29378

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLICSPVLPARWREAGICATNGISSMRIPRIHHPERLIVGSQIALSDDAANHVGRVLRMT
CEEECCCCCCCHHCCCCEEECCCCCCCCCCCCCCCEEEECCEEEECCHHHHHHHHHHHHC
AGQHLQLFDGSNQVFDAVITEAGKKNVTVEVLSGEPDDRESPLHIHLGQVMSRGEKMEFT
CCCEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEHHHHHCCCCEEEEE
IQKSIELGVSLITPLFSERCGVKLDAERLQKKIQQWQKIAIAACEQSGRNVVPEIRPAMQ
EEHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LEAWCAEQDSGLKLNLHPRASASINTLPLPVERVRLLIGPEGGLSAEEIAMTAQYQFTDI
HHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHEEEEECCCCCCCHHHHEEEEEEEEEEE
LLGPRVLRTETTALTAITALQVRFGDLG
EECCEEEEEHHHHHHHHHEEEEEECCCC
>Mature Secondary Structure
MLICSPVLPARWREAGICATNGISSMRIPRIHHPERLIVGSQIALSDDAANHVGRVLRMT
CEEECCCCCCCHHCCCCEEECCCCCCCCCCCCCCCEEEECCEEEECCHHHHHHHHHHHHC
AGQHLQLFDGSNQVFDAVITEAGKKNVTVEVLSGEPDDRESPLHIHLGQVMSRGEKMEFT
CCCEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEHHHHHCCCCEEEEE
IQKSIELGVSLITPLFSERCGVKLDAERLQKKIQQWQKIAIAACEQSGRNVVPEIRPAMQ
EEHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LEAWCAEQDSGLKLNLHPRASASINTLPLPVERVRLLIGPEGGLSAEEIAMTAQYQFTDI
HHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHEEEEECCCCCCCHHHHEEEEEEEEEEE
LLGPRVLRTETTALTAITALQVRFGDLG
EECCEEEEEHHHHHHHHHEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]