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The map label for this gene is endA [H]

Identifier: 238896475

GI number: 238896475

Start: 4457563

End: 4458327

Strand: Direct

Name: endA [H]

Synonym: KP1_4653

Alternate gene names: 238896475

Gene position: 4457563-4458327 (Clockwise)

Preceding gene: 238896474

Following gene: 238896476

Centisome position: 84.93

GC content: 55.56

Gene sequence:

>765_bases
TTGCATCCGGCACCCTCATTCGCTACGTTGCGGGCTCGTTTTGACACGGAGTTAACGATGTCCCGTATGCATGTTTTAGC
CGTAGCGGTATTGAGCGCGGCAGTCAGTGGTCCGCTTGCGGCCGCTGGTATTAATAGTTTTTCCCAGGCCAAAGCCGCTG
GCGTCAAAGTGAATGCCGACGTTCCGGGCGATTTTTATTGCGGCTGTAAAATCGACTGGCAGGGGAAAAAAGGCGTTATC
GATTTGGAATCCTGCGGCTATAAAGTGCGTAAAAATGAGAACCGCGCCAGCCGGGTTGAATGGGAGCACGTAGTGCCGGC
ATGGCAGTTCGGCCATCAGCGTCAGTGCTGGCAGGAGGGTGGACGTAAAAACTGCGCCAAAGATCCGGAATACCGCAAGA
TGGAAAGCGATATGCACAACCTGCAGCCGGCGGTGGGAGAAGTGAATGGCGATCGCGGCAACTTTATGTACAGCCAGTGG
AACGGCGGTGAAGGCCAGTACGGCCAGTGCACCATGAAGGTCGATTTTAAAGATAAAATCGCCGAGCCCCCTGCCCGCGC
TCGCGGCGCCATCGCCCGCACCTACTTCTATATGCGCGACCGCTATCAGCTGAATCTTTCCCGCCAGCAAACTCAGCTGT
TTACCGCCTGGAATAAACAGTACCCGGTCACCGCCTGGGAATGTGAACGTGATGAGCGCATCGCGAAAGTGCAGGGCAAC
CATAATCCGTATGTCCAGCAGGCTTGCCAGGCGCAAAAGAGCTAA

Upstream 100 bases:

>100_bases
GTTCGCGGCGAGGCCACCTACCGCTGCGTTCGCTGCGGCGATCTGCTGGTGGCTGAAAAATAACCATCGGAATTAATTAG
AAACTTTCTGATCTGACTGA

Downstream 100 bases:

>100_bases
CCTACACTAGCGGCAATTGTTAATTTGCAGCCCTGTGCTTCCCGCCCGATGGCGGGAAGCTGGCATCTGCGCAACGAACG
GAATATCAAGCATGCGCATT

Product: DNA-specific endonuclease I

Products: NA

Alternate protein names: Endonuclease I; Endo I [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MHPAPSFATLRARFDTELTMSRMHVLAVAVLSAAVSGPLAAAGINSFSQAKAAGVKVNADVPGDFYCGCKIDWQGKKGVI
DLESCGYKVRKNENRASRVEWEHVVPAWQFGHQRQCWQEGGRKNCAKDPEYRKMESDMHNLQPAVGEVNGDRGNFMYSQW
NGGEGQYGQCTMKVDFKDKIAEPPARARGAIARTYFYMRDRYQLNLSRQQTQLFTAWNKQYPVTAWECERDERIAKVQGN
HNPYVQQACQAQKS

Sequences:

>Translated_254_residues
MHPAPSFATLRARFDTELTMSRMHVLAVAVLSAAVSGPLAAAGINSFSQAKAAGVKVNADVPGDFYCGCKIDWQGKKGVI
DLESCGYKVRKNENRASRVEWEHVVPAWQFGHQRQCWQEGGRKNCAKDPEYRKMESDMHNLQPAVGEVNGDRGNFMYSQW
NGGEGQYGQCTMKVDFKDKIAEPPARARGAIARTYFYMRDRYQLNLSRQQTQLFTAWNKQYPVTAWECERDERIAKVQGN
HNPYVQQACQAQKS
>Mature_254_residues
MHPAPSFATLRARFDTELTMSRMHVLAVAVLSAAVSGPLAAAGINSFSQAKAAGVKVNADVPGDFYCGCKIDWQGKKGVI
DLESCGYKVRKNENRASRVEWEHVVPAWQFGHQRQCWQEGGRKNCAKDPEYRKMESDMHNLQPAVGEVNGDRGNFMYSQW
NGGEGQYGQCTMKVDFKDKIAEPPARARGAIARTYFYMRDRYQLNLSRQQTQLFTAWNKQYPVTAWECERDERIAKVQGN
HNPYVQQACQAQKS

Specific function: Has double-strand break activity [H]

COG id: COG2356

COG function: function code L; Endonuclease I

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endA/nucM nuclease family [H]

Homologues:

Organism=Escherichia coli, GI1789314, Length=235, Percent_Identity=85.9574468085106, Blast_Score=414, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007346 [H]

Pfam domain/function: PF04231 Endonuclease_1 [H]

EC number: =3.1.21.1 [H]

Molecular weight: Translated: 28721; Mature: 28721

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPAPSFATLRARFDTELTMSRMHVLAVAVLSAAVSGPLAAAGINSFSQAKAAGVKVNAD
CCCCCCHHHEEEHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCEEEECC
VPGDFYCGCKIDWQGKKGVIDLESCGYKVRKNENRASRVEWEHVVPAWQFGHQRQCWQEG
CCCCEEEEEEEECCCCCCEEEHHHCCEEEECCCCCHHHCCHHHCCCHHHCCCHHHHHHCC
GRKNCAKDPEYRKMESDMHNLQPAVGEVNGDRGNFMYSQWNGGEGQYGQCTMKVDFKDKI
CCCCCCCCCHHHHHHHHHHHCCCHHCCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHH
AEPPARARGAIARTYFYMRDRYQLNLSRQQTQLFTAWNKQYPVTAWECERDERIAKVQGN
CCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEECCC
HNPYVQQACQAQKS
CCHHHHHHHHHCCC
>Mature Secondary Structure
MHPAPSFATLRARFDTELTMSRMHVLAVAVLSAAVSGPLAAAGINSFSQAKAAGVKVNAD
CCCCCCHHHEEEHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCEEEECC
VPGDFYCGCKIDWQGKKGVIDLESCGYKVRKNENRASRVEWEHVVPAWQFGHQRQCWQEG
CCCCEEEEEEEECCCCCCEEEHHHCCEEEECCCCCHHHCCHHHCCCHHHCCCHHHHHHCC
GRKNCAKDPEYRKMESDMHNLQPAVGEVNGDRGNFMYSQWNGGEGQYGQCTMKVDFKDKI
CCCCCCCCCHHHHHHHHHHHCCCHHCCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHH
AEPPARARGAIARTYFYMRDRYQLNLSRQQTQLFTAWNKQYPVTAWECERDERIAKVQGN
CCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEECCC
HNPYVQQACQAQKS
CCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7867949; 9278503; 4955462; 8113204 [H]