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The map label for this gene is gcvH [H]

Identifier: 238896449

GI number: 238896449

Start: 4429056

End: 4429445

Strand: Reverse

Name: gcvH [H]

Synonym: KP1_4626

Alternate gene names: 238896449

Gene position: 4429445-4429056 (Counterclockwise)

Preceding gene: 238896450

Following gene: 238896448

Centisome position: 84.39

GC content: 57.18

Gene sequence:

>390_bases
ATGAGCAACGTACCAGCAGAACTGAAATACAGCAAAGAACACGAGTGGCTGCGTAAAGAAGCGGACGGTACCTATACCGT
TGGGATCACCGAGCACGCGCAGGAGCTGCTTGGCGATATGGTTTTCGTTGACCTGCCGGAAGTGGGCGCCACCGTTGAAG
CGGGCGCCGATTGCGCGGTTGCCGAATCCGTGAAGGCGGCCTCCGATATCTACGCGCCGATTAGCGGTGAAATCGTTGCC
GTCAACGAAGAGCTGAACGACTCTCCGGAACTGGTCAACAGCGACCCGTATACCGACGGCTGGATCTTCAAGATCAAAGC
CAGCGACGAAGCGCAGGTTGCCGCGCTGCTGGATGCCGCCGCGTATGAAGCTTTACTGGAAGACGAATAA

Upstream 100 bases:

>100_bases
GCAAATCCGTAACCGTGAAATGCCGGTAAAAGTGACTAAACCTGGTTTTGTACGCAATGGCAAAGCCATTGTGTGATTTC
CCTTTTTTGGAGATGAATTG

Downstream 100 bases:

>100_bases
ATACGCTTCATCCTTCACGCTGCAGGGGCGTTGGCTTCCTGGCTCCCCCCGGTCACTTACTTTTGTAAGCTCCCGGGGAG
TCGCTGTGTCGCCGCCTTCC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVA
VNEELNDSPELVNSDPYTDGWIFKIKASDEAQVAALLDAAAYEALLEDE

Sequences:

>Translated_129_residues
MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVA
VNEELNDSPELVNSDPYTDGWIFKIKASDEAQVAALLDAAAYEALLEDE
>Mature_128_residues
SNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAVAESVKAASDIYAPISGEIVAV
NEELNDSPELVNSDPYTDGWIFKIKASDEAQVAALLDAAAYEALLEDE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=42.5, Blast_Score=106, Evalue=5e-24,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=41.6666666666667, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=88.3720930232558, Blast_Score=232, Evalue=7e-63,
Organism=Caenorhabditis elegans, GI17551294, Length=121, Percent_Identity=42.9752066115703, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17507493, Length=120, Percent_Identity=43.3333333333333, Blast_Score=105, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319272, Length=128, Percent_Identity=39.84375, Blast_Score=102, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=41.6666666666667, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13861; Mature: 13730

Theoretical pI: Translated: 3.72; Mature: 3.72

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAV
CCCCCCCCEECHHHHHHHHCCCCEEEEEHHHHHHHHHHCEEEEECCCCCCEECCCCCCHH
AESVKAASDIYAPISGEIVAVNEELNDSPELVNSDPYTDGWIFKIKASDEAQVAALLDAA
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHH
AYEALLEDE
HHHHHHCCC
>Mature Secondary Structure 
SNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVEAGADCAV
CCCCCCCEECHHHHHHHHCCCCEEEEEHHHHHHHHHHCEEEEECCCCCCEECCCCCCHH
AESVKAASDIYAPISGEIVAVNEELNDSPELVNSDPYTDGWIFKIKASDEAQVAALLDAA
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHH
AYEALLEDE
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA