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The map label for this gene is kefF [H]

Identifier: 238896423

GI number: 238896423

Start: 4401849

End: 4402412

Strand: Direct

Name: kefF [H]

Synonym: KP1_4598

Alternate gene names: 238896423

Gene position: 4401849-4402412 (Clockwise)

Preceding gene: 238896422

Following gene: 238896424

Centisome position: 83.87

GC content: 56.91

Gene sequence:

>564_bases
TTGGGAATGGCTCATATTCTTGTCGTTTCCGGGCATCCGGATCTTAACCATTCCATCGCCAACGCCACCATCCTCGATGA
GCTGGCGACCGCCCTGCCTGATGCGGAAATCCGTCGTCTGGACTGGCTCTATCCGGACGGCAAATTCAACATCGCCGCTG
AGCAGGAAAGCCTGCTGCAGGCGGATGTGATTGTCTGGCAGTTTCCCTTTAGCTGGTATGGGCTGCCCGGGTTAATGAAA
CAGTGGCTGGACGAGGTATTTGTCCACGGCTTCGCCCATGGCTCAACGGCGAAACTGGGTGGTAAAAAGCTGCTCCTCTC
CTTCACCACGGGAGCGCCACAGGCGCTCTATACCGCTGACGGTTTCTTTGGCCATGCCATTGAGGAGTATCTCCTTCCCT
TCGAGACCACGGCCAGGCTGTGCAATCTTGCGCTGCTGGCGCCGGTTTATACCTGCGGTATTAGCTACGCCGACCGGGAT
GCCGACAAAATCGCTCAGCAAAAAACGCTTGCCCGGGAACACGCGGCCAGACTGATCGCGCGGCTTAATACTCTGGTGGA
ATAA

Upstream 100 bases:

>100_bases
TGGCAAAAGCGGGTCTATTAACCTTCGAGTGTATTCCACTCGGCTCGTTTATTAGCTGGGAAAATCTCTTCTCCGCTGAA
TTTAAACATCAGGAGTAACG

Downstream 100 bases:

>100_bases
CCCAAAGGGAGAAAATAACGATGCAGTATACCCGGCTCGGGAAGAGTGACTTACTGGTCTCCCGTATCTGCATGGGATGT
ATGGGGTTTGGCGATCCGTC

Product: putative NAD(P)H oxidoreductase

Products: NAD(P); reduced acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MGMAHILVVSGHPDLNHSIANATILDELATALPDAEIRRLDWLYPDGKFNIAAEQESLLQADVIVWQFPFSWYGLPGLMK
QWLDEVFVHGFAHGSTAKLGGKKLLLSFTTGAPQALYTADGFFGHAIEEYLLPFETTARLCNLALLAPVYTCGISYADRD
ADKIAQQKTLAREHAARLIARLNTLVE

Sequences:

>Translated_187_residues
MGMAHILVVSGHPDLNHSIANATILDELATALPDAEIRRLDWLYPDGKFNIAAEQESLLQADVIVWQFPFSWYGLPGLMK
QWLDEVFVHGFAHGSTAKLGGKKLLLSFTTGAPQALYTADGFFGHAIEEYLLPFETTARLCNLALLAPVYTCGISYADRD
ADKIAQQKTLAREHAARLIARLNTLVE
>Mature_186_residues
GMAHILVVSGHPDLNHSIANATILDELATALPDAEIRRLDWLYPDGKFNIAAEQESLLQADVIVWQFPFSWYGLPGLMKQ
WLDEVFVHGFAHGSTAKLGGKKLLLSFTTGAPQALYTADGFFGHAIEEYLLPFETTARLCNLALLAPVYTCGISYADRDA
DKIAQQKTLAREHAARLIARLNTLVE

Specific function: Required for full activity of kefC [H]

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily [H]

Homologues:

Organism=Homo sapiens, GI4505415, Length=118, Percent_Identity=33.0508474576271, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI156564357, Length=105, Percent_Identity=35.2380952380952, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1786231, Length=145, Percent_Identity=44.8275862068966, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1789750, Length=147, Percent_Identity=36.0544217687075, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1789406, Length=166, Percent_Identity=30.1204819277108, Blast_Score=71, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.- [C]

Molecular weight: Translated: 20639; Mature: 20508

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMAHILVVSGHPDLNHSIANATILDELATALPDAEIRRLDWLYPDGKFNIAAEQESLLQ
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCEEEEHHHHHHHH
ADVIVWQFPFSWYGLPGLMKQWLDEVFVHGFAHGSTAKLGGKKLLLSFTTGAPQALYTAD
HHEEEEECCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEECC
GFFGHAIEEYLLPFETTARLCNLALLAPVYTCGISYADRDADKIAQQKTLAREHAARLIA
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RLNTLVE
HHHHHCC
>Mature Secondary Structure 
GMAHILVVSGHPDLNHSIANATILDELATALPDAEIRRLDWLYPDGKFNIAAEQESLLQ
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCEEEEHHHHHHHH
ADVIVWQFPFSWYGLPGLMKQWLDEVFVHGFAHGSTAKLGGKKLLLSFTTGAPQALYTAD
HHEEEEECCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEECC
GFFGHAIEEYLLPFETTARLCNLALLAPVYTCGISYADRDADKIAQQKTLAREHAARLIA
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RLNTLVE
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; acceptor [C]

Specific reaction: NAD(P)H2 + acceptor = NAD(P) + reduced acceptor [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA