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The map label for this gene is gmuD [H]

Identifier: 238896358

GI number: 238896358

Start: 4337696

End: 4339123

Strand: Reverse

Name: gmuD [H]

Synonym: KP1_4523

Alternate gene names: 238896358

Gene position: 4339123-4337696 (Counterclockwise)

Preceding gene: 238896359

Following gene: 238896357

Centisome position: 82.67

GC content: 58.89

Gene sequence:

>1428_bases
ATGACTACCGTACGGATGACAATTCCCGATGATTTTATCCTTGGCGCGGCGGCTTCCGCCTGGCAGACCGAAGGCTGGAG
CGGGAAAAAACCAGGGCAGGACTCCTGGCCCGATCTCTGGTACAAAAACGATCGCCACGTCTGGCATAACGGCTATGGCC
CGGCGGTGGCCACCGATTTTATCAACCGCTTTCAGGAAGATGTGCAGCTGATGAAGCTGGCCGGGCTGACCCACTACCGC
ACCTCAATCAACTGGTCGCGCTTTCTGACCGACTACGAAAACGTCACGGTGGATGAGGAGTATGCGGCCTATTACGACCA
GCTGTTCGATGCGCTGCTGGCCAACGGCATCACGCCGATGATCTGCCTTGAGCATTATGAATTGCCCGGCTATCTGCTGG
AAAAGTATGGCGGCTGGGGGTCGAAAACGGTGGTTGAGCTGTTTGTGTGCTACGCGGAGAAGGTGTTTGCCCGCTACCAC
CCGAAGGTGACGCGCTGGTTTACCTTTAATGAGCCAATTGTGGTGCAGACCCGGGTCTATCTGGATGCTCTGCGCTGGCC
CTATGAGCAGAACACCAGCACCTGGATGCAGTGGAACTATCATAAGGTGCTGGCGACCGCCAGCGTCGTGAAGCGGTTTC
GCGAGCTCGGCTATCCCGGGACCGTCGGCTGCATCCTCAATCCGGAGGTGACCTACCCACGCTCACGCGCCCCCCATGAC
CTGCGCGCGGCGGAGATCTACGATCTGTTCTACAACCGCATGTTCCTCGACCCGCTGGTGCACGGCGTCTGGCCGCCGGA
GCTTCTCGCGCTGCTTGAGCAGCATCAGGTGACGTGGGAGACCAGCGAGGAGGATCTGGCGGTTATCCGCGAGCACACCG
TCGATGAGCTGGGGATTAATCTTTACTACCCGCACCGGGTCAAAGCCCCGTCGCGGGCCTGGCATCCGCACACCCCGTTC
CATCCGGCCTGGTATTATGAGCCGTTTGAGCTGCCCGGCCGGCGGATGAACGCCTCCCGCGGCTGGGAAATCTACCCGCA
AATCATCTTTGATATGGCGATGCGGATAAAAAATGACTATCGCAACATCCCGTGGTTCGTGGCGGAGAGCGGGATGGGGG
TGGAAAACGAAGGCCAGTTCCGTAACCGCGAAGGCGTCATCGATGACAGCTACCGCATTCGTTTTATCAGCGAACATCTG
TGGCACACCCTGCGCGCCCGCGAGGCAGGGGCCAACTGCCAGGGCTATATGCTGTGGGCGTTTACCGACAACGTATCGCC
GATGAACGCCTTTAAAAACCGCTACGGGCTTATCGAAATCGATCTGCAAAACCATCGCGCCCGGCGACCCAAAGCCTCGG
CGCACTGGTTTCGTCAACTGGGCGAACGGCGTGAACTGGTGCTGGATATCGATGATGAATACCGCTAG

Upstream 100 bases:

>100_bases
CTTATCTATCTGCCTTTCGTCAAAATCGCCTCCCGCCGTGCCGATGCCGCGCAGCGTCAGGTAGACAATCAACAAACCGC
CAACCCTGTCTGAGGAGCCT

Downstream 100 bases:

>100_bases
CCGGGGGCAATATTTGCTAAGATAGCGGCGTATTTGACTCGCAGGCATGTAACGCACAGGTGATTTTGTGGAAAAAGCGA
TAGTTCCGCCGGAAAAGAAG

Product: putative family 1 glycoside hydrolase

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTTVRMTIPDDFILGAAASAWQTEGWSGKKPGQDSWPDLWYKNDRHVWHNGYGPAVATDFINRFQEDVQLMKLAGLTHYR
TSINWSRFLTDYENVTVDEEYAAYYDQLFDALLANGITPMICLEHYELPGYLLEKYGGWGSKTVVELFVCYAEKVFARYH
PKVTRWFTFNEPIVVQTRVYLDALRWPYEQNTSTWMQWNYHKVLATASVVKRFRELGYPGTVGCILNPEVTYPRSRAPHD
LRAAEIYDLFYNRMFLDPLVHGVWPPELLALLEQHQVTWETSEEDLAVIREHTVDELGINLYYPHRVKAPSRAWHPHTPF
HPAWYYEPFELPGRRMNASRGWEIYPQIIFDMAMRIKNDYRNIPWFVAESGMGVENEGQFRNREGVIDDSYRIRFISEHL
WHTLRAREAGANCQGYMLWAFTDNVSPMNAFKNRYGLIEIDLQNHRARRPKASAHWFRQLGERRELVLDIDDEYR

Sequences:

>Translated_475_residues
MTTVRMTIPDDFILGAAASAWQTEGWSGKKPGQDSWPDLWYKNDRHVWHNGYGPAVATDFINRFQEDVQLMKLAGLTHYR
TSINWSRFLTDYENVTVDEEYAAYYDQLFDALLANGITPMICLEHYELPGYLLEKYGGWGSKTVVELFVCYAEKVFARYH
PKVTRWFTFNEPIVVQTRVYLDALRWPYEQNTSTWMQWNYHKVLATASVVKRFRELGYPGTVGCILNPEVTYPRSRAPHD
LRAAEIYDLFYNRMFLDPLVHGVWPPELLALLEQHQVTWETSEEDLAVIREHTVDELGINLYYPHRVKAPSRAWHPHTPF
HPAWYYEPFELPGRRMNASRGWEIYPQIIFDMAMRIKNDYRNIPWFVAESGMGVENEGQFRNREGVIDDSYRIRFISEHL
WHTLRAREAGANCQGYMLWAFTDNVSPMNAFKNRYGLIEIDLQNHRARRPKASAHWFRQLGERRELVLDIDDEYR
>Mature_474_residues
TTVRMTIPDDFILGAAASAWQTEGWSGKKPGQDSWPDLWYKNDRHVWHNGYGPAVATDFINRFQEDVQLMKLAGLTHYRT
SINWSRFLTDYENVTVDEEYAAYYDQLFDALLANGITPMICLEHYELPGYLLEKYGGWGSKTVVELFVCYAEKVFARYHP
KVTRWFTFNEPIVVQTRVYLDALRWPYEQNTSTWMQWNYHKVLATASVVKRFRELGYPGTVGCILNPEVTYPRSRAPHDL
RAAEIYDLFYNRMFLDPLVHGVWPPELLALLEQHQVTWETSEEDLAVIREHTVDELGINLYYPHRVKAPSRAWHPHTPFH
PAWYYEPFELPGRRMNASRGWEIYPQIIFDMAMRIKNDYRNIPWFVAESGMGVENEGQFRNREGVIDDSYRIRFISEHLW
HTLRAREAGANCQGYMLWAFTDNVSPMNAFKNRYGLIEIDLQNHRARRPKASAHWFRQLGERRELVLDIDDEYR

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=494, Percent_Identity=26.3157894736842, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI110681710, Length=484, Percent_Identity=26.0330578512397, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI13273313, Length=476, Percent_Identity=26.2605042016807, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI24497614, Length=482, Percent_Identity=23.2365145228216, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI28376633, Length=204, Percent_Identity=28.921568627451, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI2367174, Length=488, Percent_Identity=29.7131147540984, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI2367270, Length=476, Percent_Identity=29.2016806722689, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789070, Length=412, Percent_Identity=28.1553398058252, Blast_Score=152, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17539390, Length=467, Percent_Identity=27.8372591006424, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17552856, Length=472, Percent_Identity=25, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI21356577, Length=483, Percent_Identity=28.1573498964803, Blast_Score=145, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 56073; Mature: 55942

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVRMTIPDDFILGAAASAWQTEGWSGKKPGQDSWPDLWYKNDRHVWHNGYGPAVATDF
CCEEEEECCCCHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHH
INRFQEDVQLMKLAGLTHYRTSINWSRFLTDYENVTVDEEYAAYYDQLFDALLANGITPM
HHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCCEECHHHHHHHHHHHHHHHHCCCCCE
ICLEHYELPGYLLEKYGGWGSKTVVELFVCYAEKVFARYHPKVTRWFTFNEPIVVQTRVY
EEHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEHHHH
LDALRWPYEQNTSTWMQWNYHKVLATASVVKRFRELGYPGTVGCILNPEVTYPRSRAPHD
HHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
LRAAEIYDLFYNRMFLDPLVHGVWPPELLALLEQHQVTWETSEEDLAVIREHTVDELGIN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCE
LYYPHRVKAPSRAWHPHTPFHPAWYYEPFELPGRRMNASRGWEIYPQIIFDMAMRIKNDY
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
RNIPWFVAESGMGVENEGQFRNREGVIDDSYRIRFISEHLWHTLRAREAGANCQGYMLWA
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEEEE
FTDNVSPMNAFKNRYGLIEIDLQNHRARRPKASAHWFRQLGERRELVLDIDDEYR
EECCCCHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCC
>Mature Secondary Structure 
TTVRMTIPDDFILGAAASAWQTEGWSGKKPGQDSWPDLWYKNDRHVWHNGYGPAVATDF
CEEEEECCCCHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHH
INRFQEDVQLMKLAGLTHYRTSINWSRFLTDYENVTVDEEYAAYYDQLFDALLANGITPM
HHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCCEECHHHHHHHHHHHHHHHHCCCCCE
ICLEHYELPGYLLEKYGGWGSKTVVELFVCYAEKVFARYHPKVTRWFTFNEPIVVQTRVY
EEHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEHHHH
LDALRWPYEQNTSTWMQWNYHKVLATASVVKRFRELGYPGTVGCILNPEVTYPRSRAPHD
HHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
LRAAEIYDLFYNRMFLDPLVHGVWPPELLALLEQHQVTWETSEEDLAVIREHTVDELGIN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCE
LYYPHRVKAPSRAWHPHTPFHPAWYYEPFELPGRRMNASRGWEIYPQIIFDMAMRIKNDY
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
RNIPWFVAESGMGVENEGQFRNREGVIDDSYRIRFISEHLWHTLRAREAGANCQGYMLWA
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEEEE
FTDNVSPMNAFKNRYGLIEIDLQNHRARRPKASAHWFRQLGERRELVLDIDDEYR
EECCCCHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]