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The map label for this gene is pduE [H]

Identifier: 238896313

GI number: 238896313

Start: 4291294

End: 4291818

Strand: Direct

Name: pduE [H]

Synonym: KP1_4475

Alternate gene names: 238896313

Gene position: 4291294-4291818 (Clockwise)

Preceding gene: 238896312

Following gene: 238896314

Centisome position: 81.76

GC content: 59.81

Gene sequence:

>525_bases
ATGAATACCGACGCAATTGAATCCATGGTACGCGACGTGCTGAGCCGGATGAACAGCCTACAGGACGGGATAACGCCCGC
GCCAGCCGCGCCGACAAACGACACCGTTCGCCAGCCAAAAGTTAGCGACTACCCGTTAGCGACCCGCCATCCGGAGTGGG
TCAAAACCGCTACCAATAAAACGCTCGATGATCTGACGCTGGAGAACGTATTAAGCGATCGCGTTACGGCGCAGGACATG
CGCATCACTCCGGAAACGCTGCGTATGCAGGCGGCGATCGCCCAGGATGCCGGACGCGATCGGCTGGCGATGAACTTTGA
GCGGGCCGCAGAGCTCACCGCGGTTCCCGACGACCGAATCCTTGAGATCTACAACGCCCTGCGCCCATACCGTTCCACCC
AGGCGGAGCTACTGGCGATCGCTGATGACCTCGAGCATCGCTACCAGGCACGACTCTGTGCCGCCTTTGTTCGGGAAGCG
GCCGGGCTGTACATCGAGCGTAAGAAACTCAAAGGCGACGATTAA

Upstream 100 bases:

>100_bases
AGGCGAAGTCGGCCATTTTGCACATTAAAGAGACCAAATACGTGGTGACGGGCAAAAACCCGCAGGAACTGCGCGTGGCG
CTTTAACAAAGGATATCCCG

Downstream 100 bases:

>100_bases
CAGGGGGGTAAGCATGCGCTATATCGCTGGCATTGATATTGGCAACTCCTCGACAGAAGTCGCCCTGGCGACGGTCGATG
ACGCAGGTGTGCTGAACATT

Product: propanediol dehydratase small subunit

Products: NA

Alternate protein names: Propanediol utilization protein pduE [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MNTDAIESMVRDVLSRMNSLQDGITPAPAAPTNDTVRQPKVSDYPLATRHPEWVKTATNKTLDDLTLENVLSDRVTAQDM
RITPETLRMQAAIAQDAGRDRLAMNFERAAELTAVPDDRILEIYNALRPYRSTQAELLAIADDLEHRYQARLCAAFVREA
AGLYIERKKLKGDD

Sequences:

>Translated_174_residues
MNTDAIESMVRDVLSRMNSLQDGITPAPAAPTNDTVRQPKVSDYPLATRHPEWVKTATNKTLDDLTLENVLSDRVTAQDM
RITPETLRMQAAIAQDAGRDRLAMNFERAAELTAVPDDRILEIYNALRPYRSTQAELLAIADDLEHRYQARLCAAFVREA
AGLYIERKKLKGDD
>Mature_174_residues
MNTDAIESMVRDVLSRMNSLQDGITPAPAAPTNDTVRQPKVSDYPLATRHPEWVKTATNKTLDDLTLENVLSDRVTAQDM
RITPETLRMQAAIAQDAGRDRLAMNFERAAELTAVPDDRILEIYNALRPYRSTQAELLAIADDLEHRYQARLCAAFVREA
AGLYIERKKLKGDD

Specific function: Part of the pduCDE complex that catalyzes the dehydration of 1,2-propanediol to propionaldehyde. Is required for S.typhimurium growth on 1,2-propanediol as the sole carbon and energy source [H]

COG id: COG4910

COG function: function code Q; Propanediol dehydratase, small subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diol/glycerol dehydratase small subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003207 [H]

Pfam domain/function: PF02287 Dehydratase_SU [H]

EC number: =4.2.1.28 [H]

Molecular weight: Translated: 19556; Mature: 19556

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTDAIESMVRDVLSRMNSLQDGITPAPAAPTNDTVRQPKVSDYPLATRHPEWVKTATNK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCC
TLDDLTLENVLSDRVTAQDMRITPETLRMQAAIAQDAGRDRLAMNFERAAELTAVPDDRI
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHH
LEIYNALRPYRSTQAELLAIADDLEHRYQARLCAAFVREAAGLYIERKKLKGDD
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNTDAIESMVRDVLSRMNSLQDGITPAPAAPTNDTVRQPKVSDYPLATRHPEWVKTATNK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCC
TLDDLTLENVLSDRVTAQDMRITPETLRMQAAIAQDAGRDRLAMNFERAAELTAVPDDRI
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHH
LEIYNALRPYRSTQAELLAIADDLEHRYQARLCAAFVREAAGLYIERKKLKGDD
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9352910; 10498708; 11677609 [H]