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The map label for this gene is pduD [H]

Identifier: 238896312

GI number: 238896312

Start: 4290590

End: 4291279

Strand: Direct

Name: pduD [H]

Synonym: KP1_4474

Alternate gene names: 238896312

Gene position: 4290590-4291279 (Clockwise)

Preceding gene: 238896311

Following gene: 238896313

Centisome position: 81.75

GC content: 58.84

Gene sequence:

>690_bases
ATGGAAATTAACGAAACGCTGCTGCGCCAGATTATCGAAGAGGTGCTGTCGGAGATGAAATCAGGCGCAGATAAGCCGGT
CTCCTTTAGCGCGCCTGCGGCTTCTGTCGCCTCTGCCGCACCGGTCGCCGTTGCGCCTGTGTCCGGCGACAGCTTCCTGA
CGGAAATCGGCGAAGCCAAACCCGGCACGCAGCAGGATGAAGTCATTATTGCCGTCGGGCCAGCGTTTGGTCTGGCGCAA
ACCGCCAATATCGTCGGCATTCCGCATAAAAATATTCTGCGCGAAGTGATCGCCGGCATTGAGGAAGAAGGCATCAAAGC
CCGGGTGATCCGCTGCTTTAAGTCATCTGACGTCGCCTTCGTGGCAGTGGAAGGCAACCGCCTGAGCGGCTCCGGCATCT
CGATCGGTATTCAGTCGAAAGGCACCACCGTCATCCACCAGCGCGGCCTGCCGCCGCTTTCCAACCTGGAACTCTTCCCG
CAGGCGCCGCTGCTGACGCTGGAAACCTACCGTCAGATTGGCAAAAACGCCGCGCGCTACGCCAAACGCGAGTCGCCGCA
GCCGGTGCCGACGCTTAACGATCAGATGGCTCGTCCCAAATACCAGGCGAAGTCGGCCATTTTGCACATTAAAGAGACCA
AATACGTGGTGACGGGCAAAAACCCGCAGGAACTGCGCGTGGCGCTTTAA

Upstream 100 bases:

>100_bases
GGTCCGGCAACAGGCTACCGCCTGCAAGGCGAGCGCTGGGAAGAGATTAAAAATATCCCGGGCGCGCTCGATCCCAATGA
ACTTGGCTAAGGGGTGAAAA

Downstream 100 bases:

>100_bases
CAAAGGATATCCCGATGAATACCGACGCAATTGAATCCATGGTACGCGACGTGCTGAGCCGGATGAACAGCCTACAGGAC
GGGATAACGCCCGCGCCAGC

Product: propanediol dehydratase medium subunit

Products: NA

Alternate protein names: Propanediol utilization protein pduD [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MEINETLLRQIIEEVLSEMKSGADKPVSFSAPAASVASAAPVAVAPVSGDSFLTEIGEAKPGTQQDEVIIAVGPAFGLAQ
TANIVGIPHKNILREVIAGIEEEGIKARVIRCFKSSDVAFVAVEGNRLSGSGISIGIQSKGTTVIHQRGLPPLSNLELFP
QAPLLTLETYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKNPQELRVAL

Sequences:

>Translated_229_residues
MEINETLLRQIIEEVLSEMKSGADKPVSFSAPAASVASAAPVAVAPVSGDSFLTEIGEAKPGTQQDEVIIAVGPAFGLAQ
TANIVGIPHKNILREVIAGIEEEGIKARVIRCFKSSDVAFVAVEGNRLSGSGISIGIQSKGTTVIHQRGLPPLSNLELFP
QAPLLTLETYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKNPQELRVAL
>Mature_229_residues
MEINETLLRQIIEEVLSEMKSGADKPVSFSAPAASVASAAPVAVAPVSGDSFLTEIGEAKPGTQQDEVIIAVGPAFGLAQ
TANIVGIPHKNILREVIAGIEEEGIKARVIRCFKSSDVAFVAVEGNRLSGSGISIGIQSKGTTVIHQRGLPPLSNLELFP
QAPLLTLETYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKNPQELRVAL

Specific function: Part of the pduCDE complex that catalyzes the dehydration of 1,2-propanediol to propionaldehyde. Is required for S.typhimurium growth on 1,2-propanediol as the sole carbon and energy source [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diol/glycerol dehydratase medium subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010254
- InterPro:   IPR003208 [H]

Pfam domain/function: PF02288 Dehydratase_MU [H]

EC number: =4.2.1.28 [H]

Molecular weight: Translated: 24472; Mature: 24472

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEINETLLRQIIEEVLSEMKSGADKPVSFSAPAASVASAAPVAVAPVSGDSFLTEIGEAK
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCEEEEECCCCHHHHHHHCCC
PGTQQDEVIIAVGPAFGLAQTANIVGIPHKNILREVIAGIEEEGIKARVIRCFKSSDVAF
CCCCCCCEEEEECCCCCCHHCCCEEECCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEE
VAVEGNRLSGSGISIGIQSKGTTVIHQRGLPPLSNLELFPQAPLLTLETYRQIGKNAARY
EEEECCEECCCCEEEEECCCCCEEEEECCCCCCCCCEECCCCCEEHHHHHHHHHHHHHHH
AKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKNPQELRVAL
HHHCCCCCCCCCCHHHCCCHHHHHHHEEEEECCEEEEECCCHHHEEEEC
>Mature Secondary Structure
MEINETLLRQIIEEVLSEMKSGADKPVSFSAPAASVASAAPVAVAPVSGDSFLTEIGEAK
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCEEEEECCCCHHHHHHHCCC
PGTQQDEVIIAVGPAFGLAQTANIVGIPHKNILREVIAGIEEEGIKARVIRCFKSSDVAF
CCCCCCCEEEEECCCCCCHHCCCEEECCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEE
VAVEGNRLSGSGISIGIQSKGTTVIHQRGLPPLSNLELFPQAPLLTLETYRQIGKNAARY
EEEECCEECCCCEEEEECCCCCEEEEECCCCCCCCCEECCCCCEEHHHHHHHHHHHHHHH
AKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKNPQELRVAL
HHHCCCCCCCCCCHHHCCCHHHHHHHEEEEECCEEEEECCCHHHEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9352910; 10498708; 11677609 [H]