Click here to switch to the map view.

The map label for this gene is queF [H]

Identifier: 238896256

GI number: 238896256

Start: 4233929

End: 4234774

Strand: Direct

Name: queF [H]

Synonym: KP1_4409

Alternate gene names: 238896256

Gene position: 4233929-4234774 (Clockwise)

Preceding gene: 238896247

Following gene: 238896257

Centisome position: 80.67

GC content: 58.75

Gene sequence:

>846_bases
ATGTCTTCTTACGATAATCATCAGGCGCTGGCCGGCCTGACGCTCGGTAAATCAACCGATTACCGCGATACCTATGACGC
CTCGCTGCTGCAGGGCGTGCCGCGCAGTTTAAACCGCGACCCGCTGGGCCTGCATGCCGATAATCTGCCGTTTCACGGCG
CCGATATCTGGACGCTGTACGAACTCTCCTGGCTGAACGGCAAAGGTCTGCCGCAGGTGGCTGTCGGCCACGTCGAGCTG
CCCGATACCAGCCTCAATCTGGTGGAGTCGAAAAGCTTTAAGCTCTATCTCAACAGCTTTAACCAGACCCGTTTCGCCAG
CTGGCAGGACGTTGCAGAGACGCTGACTCGCGATCTCAGCGCCTGCGCGCAGGGGAAGGTTAAGGTATCGCTCTACCGCC
TGGATGAACTGGAAGGCCAGCCGATTGCCCGCCTGCACGGCACCTGCATCGACGATCAGGATATCGAGATCGATAACTAT
CAGTTCAGCGCCGATTACCTGCAGGGCGCCGCCAGCGGCAAAATCGTGGAGGAGACGCTGGTCAGCCACCTGCTGAAGTC
CAATTGCCTTATCACTCATCAGCCGGACTGGGGCTCGGTGCAGATCCAGTATCGGGGGGCGAAAATCGACCGCGAGCAGC
TCCTGCGCTATCTGGTTTCGTTCCGCCACCATAACGAATTCCATGAGCAGTGCGTCGAACGTATCTTTAACGATATTCTC
CGTTTCTGCCAGCCGGAATCGCTTTCCGTTTACGCCCGCTATACGCGACGCGGCGGTCTGGATATCAACCCCTGGCGCAG
CAATGGCGACTTTTCCCCCGCCACCGGCCGCCTCGCCCGTCAATAA

Upstream 100 bases:

>100_bases
GCGCGTAGCGCTTCTGCTGTCTGATGGTCCACCGGTTTCTCTCTTCACGTTAGACAGGATACACTGGAGCAATAGTGTAC
CCGCTTTACTATGGTGAAAC

Downstream 100 bases:

>100_bases
TAAACAATTTCACAATTTGCGTGCGTTTAGGCGCGCGCAAGGTTGTGGAAACGCCGTTCGCAGGGCTATTGTAATCACAG
GAAGACCATAAGCGTCCTGT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSSYDNHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLHADNLPFHGADIWTLYELSWLNGKGLPQVAVGHVEL
PDTSLNLVESKSFKLYLNSFNQTRFASWQDVAETLTRDLSACAQGKVKVSLYRLDELEGQPIARLHGTCIDDQDIEIDNY
QFSADYLQGAASGKIVEETLVSHLLKSNCLITHQPDWGSVQIQYRGAKIDREQLLRYLVSFRHHNEFHEQCVERIFNDIL
RFCQPESLSVYARYTRRGGLDINPWRSNGDFSPATGRLARQ

Sequences:

>Translated_281_residues
MSSYDNHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLHADNLPFHGADIWTLYELSWLNGKGLPQVAVGHVEL
PDTSLNLVESKSFKLYLNSFNQTRFASWQDVAETLTRDLSACAQGKVKVSLYRLDELEGQPIARLHGTCIDDQDIEIDNY
QFSADYLQGAASGKIVEETLVSHLLKSNCLITHQPDWGSVQIQYRGAKIDREQLLRYLVSFRHHNEFHEQCVERIFNDIL
RFCQPESLSVYARYTRRGGLDINPWRSNGDFSPATGRLARQ
>Mature_280_residues
SSYDNHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLHADNLPFHGADIWTLYELSWLNGKGLPQVAVGHVELP
DTSLNLVESKSFKLYLNSFNQTRFASWQDVAETLTRDLSACAQGKVKVSLYRLDELEGQPIARLHGTCIDDQDIEIDNYQ
FSADYLQGAASGKIVEETLVSHLLKSNCLITHQPDWGSVQIQYRGAKIDREQLLRYLVSFRHHNEFHEQCVERIFNDILR
FCQPESLSVYARYTRRGGLDINPWRSNGDFSPATGRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=282, Percent_Identity=84.3971631205674, Blast_Score=503, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 31876; Mature: 31745

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYDNHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLHADNLPFHGADIWTLY
CCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCEEEEE
ELSWLNGKGLPQVAVGHVELPDTSLNLVESKSFKLYLNSFNQTRFASWQDVAETLTRDLS
EEEECCCCCCCCEEECEEECCCCCCCCEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
ACAQGKVKVSLYRLDELEGQPIARLHGTCIDDQDIEIDNYQFSADYLQGAASGKIVEETL
HHHCCCEEEEEEEECCCCCCCCHHHCCCCCCCCCCEECCEEECHHHHHCCCCCCHHHHHH
VSHLLKSNCLITHQPDWGSVQIQYRGAKIDREQLLRYLVSFRHHNEFHEQCVERIFNDIL
HHHHHHCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
RFCQPESLSVYARYTRRGGLDINPWRSNGDFSPATGRLARQ
HHCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
SSYDNHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLHADNLPFHGADIWTLY
CCCCCCHHHEEEEECCCCCCCCHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCEEEEE
ELSWLNGKGLPQVAVGHVELPDTSLNLVESKSFKLYLNSFNQTRFASWQDVAETLTRDLS
EEEECCCCCCCCEEECEEECCCCCCCCEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
ACAQGKVKVSLYRLDELEGQPIARLHGTCIDDQDIEIDNYQFSADYLQGAASGKIVEETL
HHHCCCEEEEEEEECCCCCCCCHHHCCCCCCCCCCEECCEEECHHHHHCCCCCCHHHHHH
VSHLLKSNCLITHQPDWGSVQIQYRGAKIDREQLLRYLVSFRHHNEFHEQCVERIFNDIL
HHHHHHCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
RFCQPESLSVYARYTRRGGLDINPWRSNGDFSPATGRLARQ
HHCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA