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The map label for this gene is mazG [H]

Identifier: 238896244

GI number: 238896244

Start: 4217787

End: 4218578

Strand: Reverse

Name: mazG [H]

Synonym: KP1_4397

Alternate gene names: 238896244

Gene position: 4218578-4217787 (Counterclockwise)

Preceding gene: 238896245

Following gene: 238896243

Centisome position: 80.38

GC content: 57.58

Gene sequence:

>792_bases
ATGACTCAAATTGACCGCTTGCTCGGCATCATGCGCCGCCTGCGCGACCCGGAAAACGGCTGCCCATGGGATAAAGAGCA
GACCTTTGCCACTATCGCCCCTTACACCCTTGAGGAGACCTACGAGGTCCTCGATGCTATCCAGCGGGAAGATTTTGACG
ATCTGCGCGGTGAACTGGGCGATCTGCTGTTCCAGGTGGTGTTCTATGCGCAAATGGCGCAGGAAGAGGGACGCTTTGAC
TTTGACGATATCTGCGCCGCCATCAGCGACAAGCTGGAGCGTCGCCACCCGCATATCTTCGGCGAGGCATCGGCGGGCAA
TAGCACGGAAGTCCTGGCCCGCTGGGAGCAAATCAAAAGCGCGGAGCGAGCGGAAAAAGCCCAGCATTCGGCACTGGACG
ATATTCCCCATAGCCTGCCGGCGCTGATGCGCGCGCATAAAATCCAGCGCCGCTGCTCGGCAGTGGGTTTCGACTGGACC
TCGCTGGGCCCGGTGCTCGACAAAGTGCATGAAGAAATTGATGAAGTGATGCATGAAGCGCAGCAGGCGGTGGTGGATGA
AGCAAAACTGGAAGAGGAAGTGGGCGATCTGCTGTTTGCCACGGTGAATTTATCCCGTCATTTGGGCGTCAAAGCCGAGG
TGGCGCTGCAGAAAGCAAACCTCAAGTTTGAGCGTCGTTTCCGCGAGGTCGAGCGTATTGTCGCCGCCCGAGGCCTGGAA
ATGACCGGCGTCGACCTCGATACCATGGAAGAAGTGTGGCAGGAAGTAAAACGCCAGGAAACTGATCTCTAA

Upstream 100 bases:

>100_bases
GCCGGATAAGGCGAAGCCGCCATCCGGCAATTTCGCAACGTCGGATAAGGTCTAAACGCCTTATCCGGCCGACTATTTTT
TAAATTTCCAGGATTGTCAC

Downstream 100 bases:

>100_bases
CGGATTTTCGTGATCAAGCGCGATTTGTGTGATTTTATAAATAACAAGCGCTTGATTTACGTCAAAAACATTTCGCCAAA
AGCGTCTATTTTCTCTTTCC

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTQIDRLLGIMRRLRDPENGCPWDKEQTFATIAPYTLEETYEVLDAIQREDFDDLRGELGDLLFQVVFYAQMAQEEGRFD
FDDICAAISDKLERRHPHIFGEASAGNSTEVLARWEQIKSAERAEKAQHSALDDIPHSLPALMRAHKIQRRCSAVGFDWT
SLGPVLDKVHEEIDEVMHEAQQAVVDEAKLEEEVGDLLFATVNLSRHLGVKAEVALQKANLKFERRFREVERIVAARGLE
MTGVDLDTMEEVWQEVKRQETDL

Sequences:

>Translated_263_residues
MTQIDRLLGIMRRLRDPENGCPWDKEQTFATIAPYTLEETYEVLDAIQREDFDDLRGELGDLLFQVVFYAQMAQEEGRFD
FDDICAAISDKLERRHPHIFGEASAGNSTEVLARWEQIKSAERAEKAQHSALDDIPHSLPALMRAHKIQRRCSAVGFDWT
SLGPVLDKVHEEIDEVMHEAQQAVVDEAKLEEEVGDLLFATVNLSRHLGVKAEVALQKANLKFERRFREVERIVAARGLE
MTGVDLDTMEEVWQEVKRQETDL
>Mature_262_residues
TQIDRLLGIMRRLRDPENGCPWDKEQTFATIAPYTLEETYEVLDAIQREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDF
DDICAAISDKLERRHPHIFGEASAGNSTEVLARWEQIKSAERAEKAQHSALDDIPHSLPALMRAHKIQRRCSAVGFDWTS
LGPVLDKVHEEIDEVMHEAQQAVVDEAKLEEEVGDLLFATVNLSRHLGVKAEVALQKANLKFERRFREVERIVAARGLEM
TGVDLDTMEEVWQEVKRQETDL

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=263, Percent_Identity=87.4524714828897, Blast_Score=483, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 30149; Mature: 30018

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQIDRLLGIMRRLRDPENGCPWDKEQTFATIAPYTLEETYEVLDAIQREDFDDLRGELG
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHH
DLLFQVVFYAQMAQEEGRFDFDDICAAISDKLERRHPHIFGEASAGNSTEVLARWEQIKS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
AERAEKAQHSALDDIPHSLPALMRAHKIQRRCSAVGFDWTSLGPVLDKVHEEIDEVMHEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
QQAVVDEAKLEEEVGDLLFATVNLSRHLGVKAEVALQKANLKFERRFREVERIVAARGLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
MTGVDLDTMEEVWQEVKRQETDL
CCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQIDRLLGIMRRLRDPENGCPWDKEQTFATIAPYTLEETYEVLDAIQREDFDDLRGELG
CHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHH
DLLFQVVFYAQMAQEEGRFDFDDICAAISDKLERRHPHIFGEASAGNSTEVLARWEQIKS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
AERAEKAQHSALDDIPHSLPALMRAHKIQRRCSAVGFDWTSLGPVLDKVHEEIDEVMHEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
QQAVVDEAKLEEEVGDLLFATVNLSRHLGVKAEVALQKANLKFERRFREVERIVAARGLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
MTGVDLDTMEEVWQEVKRQETDL
CCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]