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The map label for this gene is cysJ

Identifier: 238896237

GI number: 238896237

Start: 4209646

End: 4211445

Strand: Reverse

Name: cysJ

Synonym: KP1_4389

Alternate gene names: 238896237

Gene position: 4211445-4209646 (Counterclockwise)

Preceding gene: 238896239

Following gene: 238896236

Centisome position: 80.24

GC content: 60.39

Gene sequence:

>1800_bases
ATGACGACACAGGCCCCACCTTCCAACTTGCTGCCGCTCAACCCGGAGCAACTGGCGCGCCTTCAGGCGGCCACCACTGA
CTTCACGCCCACTCAGCTCGCCTGGGTATCTGGCTATTTCTGGGGCGTGCTGAATCAGCAGTCCGGCGCGGCGGTAGCCG
CCCCCGCCCCGGCCGCCGAGGTACCGACGATCACGCTGATTTCCGCCTCGCAAACCGGCAACGCCCGCCGCGTCGCCGAA
GCGCTGCGTGACGATCTGCTGGCGGCGAAGCTGAACGTGAAGCTGGTGAATGCAGGCGATTATAAATTCAAACAAATTGC
TGCGGAAAAACTGCTGGTGGTAGTGACGTCCACCCAGGGGGAAGGAGAGCCGCCGGAAGAAGCGGTCGCATTGCATAAGT
TCCTGTTCTCTAAGAAAGCGCCGAAGCTGGACGGGACTGCGTTCGCCGTCTTTGGTCTCGGCGATACCTCTTATGAATTT
TTCTGTCAGTCCGGCAAAGATTTCGACAACAAGCTGGCCGAGCTCGGCGCGGAACGTCTGCTCGATCGCGTTGATGCCGA
CGTTGAATATCAGGCCGCGGCCGCTGAATGGCGCGCGCGCGTGGTTGAGGCGCTGAAGGCCCGTGCGCCGGTCGCTGCGC
CCGCTCAGCTGGCCACCAGCGGCGCGGTGAACGATATTCACACCAGCCCATATACCAAAGAAGCGCCCCTGACGGCGACC
CTGTCGGTCAATCAGAAAATCACCGGCCGCAATTCAGAAAAAGATGTGCGCCATATCGAAATAGACCTCGGTGATTCCGG
TCTGCGCTATCAGCCGGGCGACGCCCTGGGCGTCTGGTACCAGAACGATCCTCAGCTGGTGAAAGAGCTGGTGGAGCTGC
TGTGGCTGAAAGGCGACGAGCCGGTGACCGTTGAGGGCAAAACTCTGCCGCTGTCGGAAGCCCTGCAGTGGCATTTTGAG
CTGACGGTTAACACCGCGACTATCGTTGAAAACTACGCCACCCTGACGCGCAGCGAATCGCTGCTGCCGCTGGTGGGCGA
TAAAGCTCAGTTGCAGCAGTATGCCGCCGCGACGCCGATCGTCGATATGGTCCGTTTCTCTCCGGCGCAGCTGGACGCTG
AGGCGTTAATCGGTCTGCTGCGTCCGCTGACGCCGCGCCTGTACTCCATTGCTTCCTCACAGGCGGAAGTGGAGAGCGAG
GTGCATGTCACCGTGGGCGTGGTGCGCTATGAAATTGAAGGCCGCGCCCGGGCTGGCGGCGCCTCCAGCTTCCTCGCCGA
CCGCGTGGAAGAGGATGGCGAAGTCCGTGTCTTTATCGAACACAACGACAACTTCCGTCTGCCAGCCAACCCGGAGACCC
CGGTGATCATGATTGGCCCGGGCACCGGTATTGCGCCGTTCCGCGCCTTTATGCAGCAGCGCGCTGCGGACGGAGCGCAA
GGCAAGAACTGGTTGTTCTTCGGCAACCCGCACTTCACCGAAGATTTCCTCTATCAGGTGGAGTGGCAGAGCTATGTGAA
GGAAGGTCTGCTGACCCGTATCGATCTGGCCTGGTCTCGCGATCAACAACAAAAAATCTATGTGCAGGACAAACTGCGCG
AGCAGGGGGCCGAGCTGTGGCGCTGGATCAATGACGGCGCCCACATTTATGTCTGCGGCGACGCCAATCGTATGGCGAAG
GACGTGGAAAACACCTTACTGGAAGTGATTGCCGAATACGGCGCGATGGACGCCGAAGCGGCGGACGAATTTTTAAGTGA
GCTGCGCGTTGAGCGCCGTTATCAGCGAGATGTCTACTAA

Upstream 100 bases:

>100_bases
TTAATTGTTACTATGCCCGCCGTTAACCTTCTATTCTCTTCTGTTTTTGTCGCGCTGACAGGGTGCGAATAACAGCTTTG
CTTACTGGAACAAACGACGC

Downstream 100 bases:

>100_bases
TGAGCGAAAAACATCCGGGGCCTTTAGTGGTCGAAGGTAAACTGAGCGATGCCGAGCGCATGAAGCTTGAGAGCAACTAC
CTGCGCGGCACCATTGCGGA

Product: sulfite reductase subunit alpha

Products: NA

Alternate protein names: SiR-FP

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MTTQAPPSNLLPLNPEQLARLQAATTDFTPTQLAWVSGYFWGVLNQQSGAAVAAPAPAAEVPTITLISASQTGNARRVAE
ALRDDLLAAKLNVKLVNAGDYKFKQIAAEKLLVVVTSTQGEGEPPEEAVALHKFLFSKKAPKLDGTAFAVFGLGDTSYEF
FCQSGKDFDNKLAELGAERLLDRVDADVEYQAAAAEWRARVVEALKARAPVAAPAQLATSGAVNDIHTSPYTKEAPLTAT
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPQLVKELVELLWLKGDEPVTVEGKTLPLSEALQWHFE
LTVNTATIVENYATLTRSESLLPLVGDKAQLQQYAAATPIVDMVRFSPAQLDAEALIGLLRPLTPRLYSIASSQAEVESE
VHVTVGVVRYEIEGRARAGGASSFLADRVEEDGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADGAQ
GKNWLFFGNPHFTEDFLYQVEWQSYVKEGLLTRIDLAWSRDQQQKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAK
DVENTLLEVIAEYGAMDAEAADEFLSELRVERRYQRDVY

Sequences:

>Translated_599_residues
MTTQAPPSNLLPLNPEQLARLQAATTDFTPTQLAWVSGYFWGVLNQQSGAAVAAPAPAAEVPTITLISASQTGNARRVAE
ALRDDLLAAKLNVKLVNAGDYKFKQIAAEKLLVVVTSTQGEGEPPEEAVALHKFLFSKKAPKLDGTAFAVFGLGDTSYEF
FCQSGKDFDNKLAELGAERLLDRVDADVEYQAAAAEWRARVVEALKARAPVAAPAQLATSGAVNDIHTSPYTKEAPLTAT
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPQLVKELVELLWLKGDEPVTVEGKTLPLSEALQWHFE
LTVNTATIVENYATLTRSESLLPLVGDKAQLQQYAAATPIVDMVRFSPAQLDAEALIGLLRPLTPRLYSIASSQAEVESE
VHVTVGVVRYEIEGRARAGGASSFLADRVEEDGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADGAQ
GKNWLFFGNPHFTEDFLYQVEWQSYVKEGLLTRIDLAWSRDQQQKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAK
DVENTLLEVIAEYGAMDAEAADEFLSELRVERRYQRDVY
>Mature_598_residues
TTQAPPSNLLPLNPEQLARLQAATTDFTPTQLAWVSGYFWGVLNQQSGAAVAAPAPAAEVPTITLISASQTGNARRVAEA
LRDDLLAAKLNVKLVNAGDYKFKQIAAEKLLVVVTSTQGEGEPPEEAVALHKFLFSKKAPKLDGTAFAVFGLGDTSYEFF
CQSGKDFDNKLAELGAERLLDRVDADVEYQAAAAEWRARVVEALKARAPVAAPAQLATSGAVNDIHTSPYTKEAPLTATL
SVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPQLVKELVELLWLKGDEPVTVEGKTLPLSEALQWHFEL
TVNTATIVENYATLTRSESLLPLVGDKAQLQQYAAATPIVDMVRFSPAQLDAEALIGLLRPLTPRLYSIASSQAEVESEV
HVTVGVVRYEIEGRARAGGASSFLADRVEEDGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADGAQG
KNWLFFGNPHFTEDFLYQVEWQSYVKEGLLTRIDLAWSRDQQQKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKD
VENTLLEVIAEYGAMDAEAADEFLSELRVERRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain

Homologues:

Organism=Homo sapiens, GI127139033, Length=602, Percent_Identity=31.063122923588, Blast_Score=251, Evalue=2e-66,
Organism=Homo sapiens, GI24041029, Length=597, Percent_Identity=30.9882747068677, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI7657393, Length=595, Percent_Identity=29.5798319327731, Blast_Score=223, Evalue=6e-58,
Organism=Homo sapiens, GI221316709, Length=595, Percent_Identity=29.4117647058824, Blast_Score=216, Evalue=4e-56,
Organism=Homo sapiens, GI221316705, Length=604, Percent_Identity=29.1390728476821, Blast_Score=215, Evalue=1e-55,
Organism=Homo sapiens, GI10835173, Length=654, Percent_Identity=29.5107033639144, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI221316707, Length=524, Percent_Identity=29.9618320610687, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI40254422, Length=648, Percent_Identity=27.9320987654321, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI169790956, Length=416, Percent_Identity=28.6057692307692, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI169790958, Length=416, Percent_Identity=28.6057692307692, Blast_Score=159, Evalue=8e-39,
Organism=Escherichia coli, GI1789123, Length=599, Percent_Identity=90.3171953255426, Blast_Score=1111, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554134, Length=636, Percent_Identity=30.188679245283, Blast_Score=234, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17566446, Length=575, Percent_Identity=26.7826086956522, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17531441, Length=393, Percent_Identity=25.1908396946565, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321832, Length=640, Percent_Identity=28.59375, Blast_Score=207, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6321143, Length=392, Percent_Identity=31.6326530612245, Blast_Score=160, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6325305, Length=622, Percent_Identity=23.6334405144695, Blast_Score=137, Evalue=4e-33,
Organism=Drosophila melanogaster, GI17137192, Length=601, Percent_Identity=28.9517470881864, Blast_Score=222, Evalue=7e-58,
Organism=Drosophila melanogaster, GI24582192, Length=511, Percent_Identity=30.1369863013699, Blast_Score=202, Evalue=6e-52,
Organism=Drosophila melanogaster, GI78706872, Length=524, Percent_Identity=32.0610687022901, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24583543, Length=524, Percent_Identity=32.0610687022901, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI78706876, Length=524, Percent_Identity=32.0610687022901, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24660907, Length=593, Percent_Identity=26.3069139966273, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24660903, Length=593, Percent_Identity=26.3069139966273, Blast_Score=161, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSJ_KLEP7 (A6TD49)

Other databases:

- EMBL:   CP000647
- RefSeq:   YP_001336750.1
- ProteinModelPortal:   A6TD49
- SMR:   A6TD49
- STRING:   A6TD49
- GeneID:   5341895
- GenomeReviews:   CP000647_GR
- KEGG:   kpn:KPN_03119
- eggNOG:   COG0369
- HOGENOM:   HBG736048
- OMA:   HVEIDLG
- ProtClustDB:   PRK10953
- GO:   GO:0006810
- HAMAP:   MF_01541
- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938
- Gene3D:   G3DSA:1.20.990.10
- PIRSF:   PIRSF000207
- PRINTS:   PR00369
- PRINTS:   PR00371
- TIGRFAMs:   TIGR01931

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =1.8.1.2

Molecular weight: Translated: 66020; Mature: 65889

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS51384 FAD_FR; PS50902 FLAVODOXIN_LIKE

Important sites: BINDING 489-489

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQAPPSNLLPLNPEQLARLQAATTDFTPTQLAWVSGYFWGVLNQQSGAAVAAPAPAAE
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEECCCCCEEECCCCCCC
VPTITLISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYKFKQIAAEKLLVVVTSTQG
CCEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHCEEEEEEEECCC
EGEPPEEAVALHKFLFSKKAPKLDGTAFAVFGLGDTSYEFFCQSGKDFDNKLAELGAERL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHH
LDRVDADVEYQAAAAEWRARVVEALKARAPVAAPAQLATSGAVNDIHTSPYTKEAPLTAT
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEE
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPQLVKELVELLWLKGDE
EEECCEECCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVTVEGKTLPLSEALQWHFELTVNTATIVENYATLTRSESLLPLVGDKAQLQQYAAATPI
CEEECCCCCCHHHHCEEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCH
VDMVRFSPAQLDAEALIGLLRPLTPRLYSIASSQAEVESEVHVTVGVVRYEIEGRARAGG
HHHHHCCCHHCCHHHHHHHHHCCCHHHHHHHCCHHHHCCCEEEEEEEEEEEECCCCCCCC
ASSFLADRVEEDGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADGAQ
HHHHHHHHHCCCCCEEEEEEECCCEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
GKNWLFFGNPHFTEDFLYQVEWQSYVKEGLLTRIDLAWSRDQQQKIYVQDKLREQGAELW
CCCEEEECCCCCCHHHHEEEHHHHHHHHCHHEEEEEHCCCCCCCEEHHHHHHHHHHHHHH
RWINDGAHIYVCGDANRMAKDVENTLLEVIAEYGAMDAEAADEFLSELRVERRYQRDVY
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTQAPPSNLLPLNPEQLARLQAATTDFTPTQLAWVSGYFWGVLNQQSGAAVAAPAPAAE
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEECCCCCEEECCCCCCC
VPTITLISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYKFKQIAAEKLLVVVTSTQG
CCEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHCEEEEEEEECCC
EGEPPEEAVALHKFLFSKKAPKLDGTAFAVFGLGDTSYEFFCQSGKDFDNKLAELGAERL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHH
LDRVDADVEYQAAAAEWRARVVEALKARAPVAAPAQLATSGAVNDIHTSPYTKEAPLTAT
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEE
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPQLVKELVELLWLKGDE
EEECCEECCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVTVEGKTLPLSEALQWHFELTVNTATIVENYATLTRSESLLPLVGDKAQLQQYAAATPI
CEEECCCCCCHHHHCEEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCH
VDMVRFSPAQLDAEALIGLLRPLTPRLYSIASSQAEVESEVHVTVGVVRYEIEGRARAGG
HHHHHCCCHHCCHHHHHHHHHCCCHHHHHHHCCHHHHCCCEEEEEEEEEEEECCCCCCCC
ASSFLADRVEEDGEVRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQRAADGAQ
HHHHHHHHHCCCCCEEEEEEECCCEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
GKNWLFFGNPHFTEDFLYQVEWQSYVKEGLLTRIDLAWSRDQQQKIYVQDKLREQGAELW
CCCEEEECCCCCCHHHHEEEHHHHHHHHCHHEEEEEHCCCCCCCEEHHHHHHHHHHHHHH
RWINDGAHIYVCGDANRMAKDVENTLLEVIAEYGAMDAEAADEFLSELRVERRYQRDVY
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA