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The map label for this gene is celB [H]

Identifier: 238896192

GI number: 238896192

Start: 4165849

End: 4167249

Strand: Reverse

Name: celB [H]

Synonym: KP1_4340

Alternate gene names: 238896192

Gene position: 4167249-4165849 (Counterclockwise)

Preceding gene: 238896202

Following gene: 238896191

Centisome position: 79.4

GC content: 54.53

Gene sequence:

>1401_bases
ATGCATTGCGGAAAGCGCTTTCCGTTTGTGGATTTATTAATTGTGACCATGATCACCTATGGCAGGCTTTCACTTTCCGG
TGTTGTGGGCGATCATCCCGGCGAGACAACCATTCGCAAAGGTAGGAGTATGAACATTCAGTTAATGAATGTGGCCGTGG
ACATCATCGAGCAGCGGCTGGCGCCGCTGGCGAACGTTCTGACGCGCAACAACCACATTACGGCGATGCGCGATAGCTTT
GCGCTGGCTATGCCCTTTGTGATTGTTGGCAGCCTGTTAGTTCCCATTTTGTTTCCGCCCGTTTCCATCGACGGCGCCTC
GCGTTTCGGCCAGGTCTATTTGCTGCTGCGGCCTATCCTGCTGCCGACCTTTCAGCTAACTATTGGCCTGGTGGCGCTGA
TTGTCGCCTTCGGCGCCAGCGCCAGTCTCGCCAAGCAGTACCGTCTGCCGGAACGGCTCTGCGGTTTAACCGGGTGTCTG
GCTTTTCTGCTGTTTATCGGCTTTCGCGAGACTGCGGTGAGCAATGTTTATCTCGGCGGTATGGGGATATTTACCGCCCT
GATATCCAGCACCTATTCCATTGAGTTAATTCGCTTTTTCTACAAAAAAGGCTGGTGCATTCGCTTGCCTGATGAAGTGC
CGCTGATGACCCGCAATGGCTTTCAACTGCTGATCCCGCTGCTGGTGGTGATGCTGTCGATAAGCGTGATGAATGCCATA
CTGCTGCAGACCACCGGACGCATCGTACCGGAGTTGATCAGCGAAGCGGTGCGGCCGCTGGTGCTGGCGTCCGACACGCT
GATGGCGGTACTTATCTCTTTATTTATCTGTAACTTACTCTGGTTTATCGGTATCCACGGCGCGCTGATCATCACCGGGA
TCATGAACCCCTTCTGGATGACCTATCTGTTTGAAAATCAGCAGGCGCTGGCGGCGGGTTCGCCAACGCTGCCGCATATT
TATCTGCAGGGGTTCTGGGATTTTTACCTGCTGATCGGCGGTATTGGCTCCACTTTGCCGCTGGTGCTGATGGCGATGCG
CAGCCGCTCGCGGCAGCTGAAAAGCGTGGCCAAGATTGGCCTGCTGCCGTCGCTGTTTAATATCAACGAGCCGATACTGT
TTGGTTTTCCGGTGATCATGAACCCGGTTTTTTTACTGCCGTTTCTCTTTGTTCCCCTGATAAACGCCTGTATCGCATGG
TATTTAACTCAGCTGGGTATTCTCGATCGCGCGGTGGCGATGCTGCCATGGTCGATGCCTTCCCCGCTGGGGGCGGCATG
GTCGGCGAACGGCAGCTGGAAAAATTTGTGCATGAGCCTGTTCGCGATGTTTAACGCCTGGATGCTCTATCGCCCCTTCT
TCAAAGTGTACGAACGCCAGCTCGCGGAAACCGAACGCTGA

Upstream 100 bases:

>100_bases
TCAGCAGGCTGGTGGACATGCCTGCCGAACAACACAACATAATTTTTTTCATAATTACCGCTCCTGATGAGGTTGGATGA
GCGGACTATAGTCAAAATGG

Downstream 100 bases:

>100_bases
CCCCCTCGCCGTCGGCGGCTTATCCCCCGGCGGCACCTGCTCATCTGCTCCTCCTTCCGCTCCACTGCCGGCTCCCGGCG
GCATTCTTGTAATTTTATGT

Product: PEP-dependent phosphotransferase enzyme II

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIC-Cel; PTS system cellobiose-specific EIIC component [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MHCGKRFPFVDLLIVTMITYGRLSLSGVVGDHPGETTIRKGRSMNIQLMNVAVDIIEQRLAPLANVLTRNNHITAMRDSF
ALAMPFVIVGSLLVPILFPPVSIDGASRFGQVYLLLRPILLPTFQLTIGLVALIVAFGASASLAKQYRLPERLCGLTGCL
AFLLFIGFRETAVSNVYLGGMGIFTALISSTYSIELIRFFYKKGWCIRLPDEVPLMTRNGFQLLIPLLVVMLSISVMNAI
LLQTTGRIVPELISEAVRPLVLASDTLMAVLISLFICNLLWFIGIHGALIITGIMNPFWMTYLFENQQALAAGSPTLPHI
YLQGFWDFYLLIGGIGSTLPLVLMAMRSRSRQLKSVAKIGLLPSLFNINEPILFGFPVIMNPVFLLPFLFVPLINACIAW
YLTQLGILDRAVAMLPWSMPSPLGAAWSANGSWKNLCMSLFAMFNAWMLYRPFFKVYERQLAETER

Sequences:

>Translated_466_residues
MHCGKRFPFVDLLIVTMITYGRLSLSGVVGDHPGETTIRKGRSMNIQLMNVAVDIIEQRLAPLANVLTRNNHITAMRDSF
ALAMPFVIVGSLLVPILFPPVSIDGASRFGQVYLLLRPILLPTFQLTIGLVALIVAFGASASLAKQYRLPERLCGLTGCL
AFLLFIGFRETAVSNVYLGGMGIFTALISSTYSIELIRFFYKKGWCIRLPDEVPLMTRNGFQLLIPLLVVMLSISVMNAI
LLQTTGRIVPELISEAVRPLVLASDTLMAVLISLFICNLLWFIGIHGALIITGIMNPFWMTYLFENQQALAAGSPTLPHI
YLQGFWDFYLLIGGIGSTLPLVLMAMRSRSRQLKSVAKIGLLPSLFNINEPILFGFPVIMNPVFLLPFLFVPLINACIAW
YLTQLGILDRAVAMLPWSMPSPLGAAWSANGSWKNLCMSLFAMFNAWMLYRPFFKVYERQLAETER
>Mature_466_residues
MHCGKRFPFVDLLIVTMITYGRLSLSGVVGDHPGETTIRKGRSMNIQLMNVAVDIIEQRLAPLANVLTRNNHITAMRDSF
ALAMPFVIVGSLLVPILFPPVSIDGASRFGQVYLLLRPILLPTFQLTIGLVALIVAFGASASLAKQYRLPERLCGLTGCL
AFLLFIGFRETAVSNVYLGGMGIFTALISSTYSIELIRFFYKKGWCIRLPDEVPLMTRNGFQLLIPLLVVMLSISVMNAI
LLQTTGRIVPELISEAVRPLVLASDTLMAVLISLFICNLLWFIGIHGALIITGIMNPFWMTYLFENQQALAAGSPTLPHI
YLQGFWDFYLLIGGIGSTLPLVLMAMRSRSRQLKSVAKIGLLPSLFNINEPILFGFPVIMNPVFLLPFLFVPLINACIAW
YLTQLGILDRAVAMLPWSMPSPLGAAWSANGSWKNLCMSLFAMFNAWMLYRPFFKVYERQLAETER

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=443, Percent_Identity=30.4740406320542, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 51850; Mature: 51850

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHCGKRFPFVDLLIVTMITYGRLSLSGVVGDHPGETTIRKGRSMNIQLMNVAVDIIEQRL
CCCCCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHCCCCCCEEHHHHHHHHHHHHH
APLANVLTRNNHITAMRDSFALAMPFVIVGSLLVPILFPPVSIDGASRFGQVYLLLRPIL
HHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LPTFQLTIGLVALIVAFGASASLAKQYRLPERLCGLTGCLAFLLFIGFRETAVSNVYLGG
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECH
MGIFTALISSTYSIELIRFFYKKGWCIRLPDEVPLMTRNGFQLLIPLLVVMLSISVMNAI
HHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LLQTTGRIVPELISEAVRPLVLASDTLMAVLISLFICNLLWFIGIHGALIITGIMNPFWM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYLFENQQALAAGSPTLPHIYLQGFWDFYLLIGGIGSTLPLVLMAMRSRSRQLKSVAKIG
HHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLPSLFNINEPILFGFPVIMNPVFLLPFLFVPLINACIAWYLTQLGILDRAVAMLPWSMP
CCHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SPLGAAWSANGSWKNLCMSLFAMFNAWMLYRPFFKVYERQLAETER
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHCGKRFPFVDLLIVTMITYGRLSLSGVVGDHPGETTIRKGRSMNIQLMNVAVDIIEQRL
CCCCCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHCCCCCCEEHHHHHHHHHHHHH
APLANVLTRNNHITAMRDSFALAMPFVIVGSLLVPILFPPVSIDGASRFGQVYLLLRPIL
HHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LPTFQLTIGLVALIVAFGASASLAKQYRLPERLCGLTGCLAFLLFIGFRETAVSNVYLGG
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECH
MGIFTALISSTYSIELIRFFYKKGWCIRLPDEVPLMTRNGFQLLIPLLVVMLSISVMNAI
HHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LLQTTGRIVPELISEAVRPLVLASDTLMAVLISLFICNLLWFIGIHGALIITGIMNPFWM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYLFENQQALAAGSPTLPHIYLQGFWDFYLLIGGIGSTLPLVLMAMRSRSRQLKSVAKIG
HHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLPSLFNINEPILFGFPVIMNPVFLLPFLFVPLINACIAWYLTQLGILDRAVAMLPWSMP
CCHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SPLGAAWSANGSWKNLCMSLFAMFNAWMLYRPFFKVYERQLAETER
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8407820 [H]