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The map label for this gene is hydN [H]

Identifier: 238896169

GI number: 238896169

Start: 4141280

End: 4141807

Strand: Reverse

Name: hydN [H]

Synonym: KP1_4315

Alternate gene names: 238896169

Gene position: 4141807-4141280 (Counterclockwise)

Preceding gene: 238896170

Following gene: 238896168

Centisome position: 78.91

GC content: 60.8

Gene sequence:

>528_bases
ATGAACCGTTTCATTATCGCCGATGCGAGCAAATGTATCGGCTGTCGCACTTGCGAAGTGGCCTGCGTGGTATCCCATCA
GGAACATCAGGACTGCGCCTCCCTGACGCCTCACACCTTTCAGCCGCGTATTCACGTGATCAAAGGGGTCAATGTTTCCA
CGGCGACCGCCTGCCGTCAGTGTGAAGACGCCCCCTGCGCCAATGTCTGCCCGAACGGCGCCATCAGCCGCGATAAAGGC
TTTGTCCATGTGATGCAGGAGCGTTGCATCGGCTGCAAAACCTGCGTCGTCGCCTGCCCGTACGGGGCGATGGAGGTGGT
GGTGCGCCCGGTGATCCGCCATAGCGGCGCCGGACTGAACGTCGTGGCGGAAAAAGCCGAAGCCAACAAGTGCGATCTCT
GCTTCCACCGCGAGAGCGGCCCGGCCTGCATGGAAGTGTGCCCGACGCACGCCCTCGTGTGCGTTGACCGCAACAAACTT
GAGCAGATGAATATCGAGAAACGTCGCCGTACGGCGCTGGCCTGGTAA

Upstream 100 bases:

>100_bases
GCAAACCGCAGCTATAATAATCTTTATAATCAATATATTAAAAACTTGGAACGTATTATGCATACTCCGGCGCGTAACCT
TCTTACTGCTGGAGAAATGG

Downstream 100 bases:

>100_bases
GTCAAGGCATGAACGGGGTGCAAATCCGCATTCGCGGTAAAGTGCAGGGGGTCGGTTTCCGCCCCTTTGTCTGGCAGCTG
GCCCGGGCGCAGGCCCGCTG

Product: electron transport protein HydN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MNRFIIADASKCIGCRTCEVACVVSHQEHQDCASLTPHTFQPRIHVIKGVNVSTATACRQCEDAPCANVCPNGAISRDKG
FVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRHSGAGLNVVAEKAEANKCDLCFHRESGPACMEVCPTHALVCVDRNKL
EQMNIEKRRRTALAW

Sequences:

>Translated_175_residues
MNRFIIADASKCIGCRTCEVACVVSHQEHQDCASLTPHTFQPRIHVIKGVNVSTATACRQCEDAPCANVCPNGAISRDKG
FVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRHSGAGLNVVAEKAEANKCDLCFHRESGPACMEVCPTHALVCVDRNKL
EQMNIEKRRRTALAW
>Mature_175_residues
MNRFIIADASKCIGCRTCEVACVVSHQEHQDCASLTPHTFQPRIHVIKGVNVSTATACRQCEDAPCANVCPNGAISRDKG
FVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRHSGAGLNVVAEKAEANKCDLCFHRESGPACMEVCPTHALVCVDRNKL
EQMNIEKRRRTALAW

Specific function: Electron transport from formate to hydrogen [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=175, Percent_Identity=89.7142857142857, Blast_Score=323, Evalue=4e-90,
Organism=Escherichia coli, GI2367245, Length=168, Percent_Identity=59.5238095238095, Blast_Score=194, Evalue=3e-51,
Organism=Escherichia coli, GI1788811, Length=172, Percent_Identity=50.5813953488372, Blast_Score=161, Evalue=2e-41,
Organism=Escherichia coli, GI87082180, Length=173, Percent_Identity=40.4624277456647, Blast_Score=133, Evalue=7e-33,
Organism=Escherichia coli, GI87082179, Length=167, Percent_Identity=39.5209580838323, Blast_Score=124, Evalue=3e-30,
Organism=Escherichia coli, GI1789079, Length=190, Percent_Identity=38.9473684210526, Blast_Score=120, Evalue=4e-29,
Organism=Escherichia coli, GI87082114, Length=197, Percent_Identity=34.010152284264, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1787872, Length=166, Percent_Identity=33.7349397590361, Blast_Score=83, Evalue=8e-18,
Organism=Escherichia coli, GI1787122, Length=166, Percent_Identity=33.7349397590361, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI2367345, Length=155, Percent_Identity=33.5483870967742, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI226510944, Length=161, Percent_Identity=33.5403726708075, Blast_Score=73, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 19153; Mature: 19153

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
13.1 %Cys+Met (Translated Protein)
10.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
13.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFIIADASKCIGCRTCEVACVVSHQEHQDCASLTPHTFQPRIHVIKGVNVSTATACRQ
CCEEEEECCCHHCCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHH
CEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRHSGAGLN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHHCCCCCE
VVAEKAEANKCDLCFHRESGPACMEVCPTHALVCVDRNKLEQMNIEKRRRTALAW
EEEECCCCCCCEEEEECCCCCHHHHHCCCCEEEEECCCHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MNRFIIADASKCIGCRTCEVACVVSHQEHQDCASLTPHTFQPRIHVIKGVNVSTATACRQ
CCEEEEECCCHHCCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHH
CEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRHSGAGLN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHHCCCCCE
VVAEKAEANKCDLCFHRESGPACMEVCPTHALVCVDRNKLEQMNIEKRRRTALAW
EEEECCCCCCCEEEEECCCCCHHHHHCCCCEEEEECCCHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]