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The map label for this gene is kgtP [H]

Identifier: 238896036

GI number: 238896036

Start: 3998649

End: 3999947

Strand: Reverse

Name: kgtP [H]

Synonym: KP1_4167

Alternate gene names: 238896036

Gene position: 3999947-3998649 (Counterclockwise)

Preceding gene: 238896038

Following gene: 238896030

Centisome position: 76.21

GC content: 57.04

Gene sequence:

>1299_bases
ATGACAGAAAGTAGCATTACCGAACGGGGCGCGCCGGAACTGGCCGATACCCGTCGTCGTATTTGGGCAATTGTTGGCGC
TTCCTCGGGGAATCTGGTTGAATGGTTTGATTTTTACGTTTACTCATTCTGCTCGCTCTATTTCGCGCACATCTTCTTTC
CATCCGGAAATACCACCACCCAACTGCTGCAAACGGCAGGCGTATTTGCCGCCGGATTTCTGATGCGTCCTATCGGTGGA
TGGCTGTTTGGTCGGATCGCTGACCGCCGGGGACGTAAAACGTCCATGCTTATCTCGGTCTGCATGATGTGCTTTGGCTC
ACTGGTGATTGCCTGTCTGCCGGGTTATGCGGTGATCGGCACCTGGGCCCCGGCGCTGCTGCTGCTGGCGCGTCTGTTCC
AGGGGCTGTCTGTTGGCGGAGAATACGGCACCAGTGCGACCTATATGAGTGAAGTCGCGGTGGAGGGTAAGAAAGGCTTC
TATGCCTCCTTCCAGTACGTTACGCTGATTGGTGGTCAACTGCTGGCTGTATTAGTGGTTGTGGCGCTCCAGCAGGTGCT
TAGCGATGAAGATCTCCATGCCTGGGGATGGCGTATTCCTTTTGCCCTCGGGGCGGTACTGGCTATCGTCGCGCTGTGGC
TGCGTCGCCAGCTGGACGAAACCTCGAAGCAGGAGACCCGGGCGCTGAAAGAGGCTGGCTCATTTAAAGGGTTGTGGCGC
AATCGCCGGGCCTTTGCGATGGTGTTAGGCTTTACCGCCGCCGGATCGCTGACCTTCTACACCTTCACCACCTATATGCA
AAAGTATCTGGTCAACACGGCGGGCATGACGGCCAGCACCGCCAGCGTGATCATGACCGCGGCGCTGTTCGTTTATATGC
TGGTTCAGCCGCTATTCGGCGCCTTCTCGGATAAAGTTGGCCGTCGGACCTCCATGCTGTGCTTTGGCGTGCTGGCGACG
TTATTTACCGTGCCTATTCTCAGCGCCTTGCAAAAAGTGAGCTCACCCTATGCCGCCTTCGGCCTGGTGATCTGCGCCTT
ACTGATTGTCAGTTTCTATACCTCGATCAGCGGCATCCTGAAAGCAGAGATGTTCCCGGCTCAGGTTCGCGCGCTTGGGG
TCGGGTTGTCCTATGCCGTCGCGAACGCATTGTTCGGCGGCTCTGCGGAGTACGTCGCGCTGTCGCTTAAGTCGGCAGGG
ATAGAGCACGCGTTCTACTGGTATGTGACGGTGATGGCCGCCATTGCCTTCCTTGTCTCCCTGATGTTGCATCGCAAAGG
GAAGGGGTTGCGTCTGTAA

Upstream 100 bases:

>100_bases
ATATAACAATTATATAACATTTAGTTTACTAATTAATTTGTCTGCGTGACGTGGTACGCCCGACAAGACAGATTGCAGTC
TTTGCTGGAGCAATATAACA

Downstream 100 bases:

>100_bases
CGCTTACTGATGAGCCAACTGCCATACAGCGTAGCCGGTGGTCGCGCCGGCGACATCCCAGGCAAAATCCTTCCAGCTCC
AGCCGCTCCCAGCCGGGCGG

Product: alpha-ketoglutarate transporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTESSITERGAPELADTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGG
WLFGRIADRRGRKTSMLISVCMMCFGSLVIACLPGYAVIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGKKGF
YASFQYVTLIGGQLLAVLVVVALQQVLSDEDLHAWGWRIPFALGAVLAIVALWLRRQLDETSKQETRALKEAGSFKGLWR
NRRAFAMVLGFTAAGSLTFYTFTTYMQKYLVNTAGMTASTASVIMTAALFVYMLVQPLFGAFSDKVGRRTSMLCFGVLAT
LFTVPILSALQKVSSPYAAFGLVICALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANALFGGSAEYVALSLKSAG
IEHAFYWYVTVMAAIAFLVSLMLHRKGKGLRL

Sequences:

>Translated_432_residues
MTESSITERGAPELADTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGG
WLFGRIADRRGRKTSMLISVCMMCFGSLVIACLPGYAVIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGKKGF
YASFQYVTLIGGQLLAVLVVVALQQVLSDEDLHAWGWRIPFALGAVLAIVALWLRRQLDETSKQETRALKEAGSFKGLWR
NRRAFAMVLGFTAAGSLTFYTFTTYMQKYLVNTAGMTASTASVIMTAALFVYMLVQPLFGAFSDKVGRRTSMLCFGVLAT
LFTVPILSALQKVSSPYAAFGLVICALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANALFGGSAEYVALSLKSAG
IEHAFYWYVTVMAAIAFLVSLMLHRKGKGLRL
>Mature_431_residues
TESSITERGAPELADTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGW
LFGRIADRRGRKTSMLISVCMMCFGSLVIACLPGYAVIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGKKGFY
ASFQYVTLIGGQLLAVLVVVALQQVLSDEDLHAWGWRIPFALGAVLAIVALWLRRQLDETSKQETRALKEAGSFKGLWRN
RRAFAMVLGFTAAGSLTFYTFTTYMQKYLVNTAGMTASTASVIMTAALFVYMLVQPLFGAFSDKVGRRTSMLCFGVLATL
FTVPILSALQKVSSPYAAFGLVICALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANALFGGSAEYVALSLKSAGI
EHAFYWYVTVMAAIAFLVSLMLHRKGKGLRL

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=432, Percent_Identity=87.037037037037, Blast_Score=730, Evalue=0.0,
Organism=Escherichia coli, GI1790550, Length=380, Percent_Identity=31.8421052631579, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1788292, Length=434, Percent_Identity=30.6451612903226, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789941, Length=441, Percent_Identity=32.4263038548753, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87082231, Length=315, Percent_Identity=27.3015873015873, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082404, Length=371, Percent_Identity=23.4501347708895, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46986; Mature: 46855

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESSITERGAPELADTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTT
CCCCCCCCCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
QLLQTAGVFAAGFLMRPIGGWLFGRIADRRGRKTSMLISVCMMCFGSLVIACLPGYAVIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
TWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGKKGFYASFQYVTLIGGQLLAVLVV
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VALQQVLSDEDLHAWGWRIPFALGAVLAIVALWLRRQLDETSKQETRALKEAGSFKGLWR
HHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
NRRAFAMVLGFTAAGSLTFYTFTTYMQKYLVNTAGMTASTASVIMTAALFVYMLVQPLFG
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AFSDKVGRRTSMLCFGVLATLFTVPILSALQKVSSPYAAFGLVICALLIVSFYTSISGIL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KAEMFPAQVRALGVGLSYAVANALFGGSAEYVALSLKSAGIEHAFYWYVTVMAAIAFLVS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEHHHCCCHHHHHHHHHHHHHHHHHHH
LMLHRKGKGLRL
HHHHCCCCCCCC
>Mature Secondary Structure 
TESSITERGAPELADTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTT
CCCCCCCCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH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HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]