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The map label for this gene is yfiF [H]

Identifier: 238896030

GI number: 238896030

Start: 3991636

End: 3992721

Strand: Reverse

Name: yfiF [H]

Synonym: KP1_4161

Alternate gene names: 238896030

Gene position: 3992721-3991636 (Counterclockwise)

Preceding gene: 238896036

Following gene: 238896028

Centisome position: 76.07

GC content: 59.58

Gene sequence:

>1086_bases
ATGAGCGACGAACTGAAAAATAAAAGCGGCAAGGTCAAAGTGATGTATGTCCGCAGTGATGATGATTCTGACAAACGCAC
CCATAACCCACGTACCGGGAAAGGGGGAGGTCGTCCAGGAAAATCTCGTGTTGACGGCCGCAGCCGCCCTGCGCGTGACG
AACGAAACAGCCGCGGCGACGATCGCAAACGTGACGACCGTAAGCGTGACGAGCGCAAACGCGATGATTTTCATCGCGAC
GATGCGTCTCCGTGGCGTACCGTTTCCCGCGCTCCTGGCGATGAGACGCCGGTAAAAGAAGATCACGGCGGGATCAGCGG
GAAGAGCTTTATCGATCCGGAAGTGCTGCGTCGTCAGCGTGCGGAAGAGACCCGCGTATATGGCGAAAACGCCTGCCAGG
CGCTGTTCCAGAGCCGTCCGGAAGCTATCGTTCGCGCCTGGTTTATTCAGAGCGTGACGCCGCGCTTTAAAGAGGCGCTG
CGCTGGATGGCGGCCAACCGTAAGGCCTATCACGTGGTGGACGAAGCCGAGCTGGCGAAAGCCTCCGGCACCGAGCACCA
CGGCGGCGTCTGCTTCCTCATCAAAAAACGCCACGGCACTTCTGTTGCTCAATGGGTCGCGAAGGCCGGGGAAGAAGATT
GCGTTCTGGCGCTGGAAGATGTGGGTAACCCACATAATCTCGGGGCGATCATGCGTAGCTGCGCGCACTTTGGCGTGAAG
GGCGTGGTGGTTCAGGATGCTGGCGTGCTGGAGTCCGGGGCGGCGATCCGTACGGCGGAGGGCGGTGCGGAGCATGTTGA
ACCGATCACCGGCGACAGCTTTATCGATACCCTCGATCAGTTCCGCAAAGCGGGCTACGCGATTGTCTCAACGTCCAGCC
ATAATGGTACCCCGCTGTTTAAAGCCGAGCTGCCGAAGAAAATGGTGCTGGCGTTAGGCCAGGAACGTGATGGTCTGTCC
GATGCGGCGATTTCCAGCGCCGATCTCAGCGTTGCCATCGACGGTACCGGTAATGTCGAAAGCCTGAACGTCTCTGTGGC
GACCGGCGTCCTGCTGGCGGAATGGTGGCGTCAGAACAAGGCGTGA

Upstream 100 bases:

>100_bases
CGATGAGTGCAATAGAGGCCGGCTTTTAAACGAAATCATGGCGAAGCGATGGCACATCGGGTAATCTGCGCGCTTCGCGC
AGCGCAGGTGGAGAAAATTG

Downstream 100 bases:

>100_bases
TTCGCCTGCCTGAATGCAAAAAAGCCAGCTGATGCTGGCTTTTTTTATGGCTGTCAATCACTCGCTTTCAGCGGGAAGGA
CCGGCATCCAGTCGATCGGC

Product: putative methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSDELKNKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRVDGRSRPARDERNSRGDDRKRDDRKRDERKRDDFHRD
DASPWRTVSRAPGDETPVKEDHGGISGKSFIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEAL
RWMAANRKAYHVVDEAELAKASGTEHHGGVCFLIKKRHGTSVAQWVAKAGEEDCVLALEDVGNPHNLGAIMRSCAHFGVK
GVVVQDAGVLESGAAIRTAEGGAEHVEPITGDSFIDTLDQFRKAGYAIVSTSSHNGTPLFKAELPKKMVLALGQERDGLS
DAAISSADLSVAIDGTGNVESLNVSVATGVLLAEWWRQNKA

Sequences:

>Translated_361_residues
MSDELKNKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRVDGRSRPARDERNSRGDDRKRDDRKRDERKRDDFHRD
DASPWRTVSRAPGDETPVKEDHGGISGKSFIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEAL
RWMAANRKAYHVVDEAELAKASGTEHHGGVCFLIKKRHGTSVAQWVAKAGEEDCVLALEDVGNPHNLGAIMRSCAHFGVK
GVVVQDAGVLESGAAIRTAEGGAEHVEPITGDSFIDTLDQFRKAGYAIVSTSSHNGTPLFKAELPKKMVLALGQERDGLS
DAAISSADLSVAIDGTGNVESLNVSVATGVLLAEWWRQNKA
>Mature_360_residues
SDELKNKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRVDGRSRPARDERNSRGDDRKRDDRKRDERKRDDFHRDD
ASPWRTVSRAPGDETPVKEDHGGISGKSFIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALR
WMAANRKAYHVVDEAELAKASGTEHHGGVCFLIKKRHGTSVAQWVAKAGEEDCVLALEDVGNPHNLGAIMRSCAHFGVKG
VVVQDAGVLESGAAIRTAEGGAEHVEPITGDSFIDTLDQFRKAGYAIVSTSSHNGTPLFKAELPKKMVLALGQERDGLSD
AAISSADLSVAIDGTGNVESLNVSVATGVLLAEWWRQNKA

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=361, Percent_Identity=78.393351800554, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1790623, Length=238, Percent_Identity=26.890756302521, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790083, Length=155, Percent_Identity=29.0322580645161, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 39608; Mature: 39476

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDELKNKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRVDGRSRPARDERNSRGD
CCCCCCCCCCCEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCC
DRKRDDRKRDERKRDDFHRDDASPWRTVSRAPGDETPVKEDHGGISGKSFIDPEVLRRQR
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEAELAK
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEECCHHHHHH
ASGTEHHGGVCFLIKKRHGTSVAQWVAKAGEEDCVLALEDVGNPHNLGAIMRSCAHFGVK
HCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEHHHCCCCHHHHHHHHHHHHCCCC
GVVVQDAGVLESGAAIRTAEGGAEHVEPITGDSFIDTLDQFRKAGYAIVSTSSHNGTPLF
EEEEECCCCCCCCCEEEECCCCHHHCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCEEH
KAELPKKMVLALGQERDGLSDAAISSADLSVAIDGTGNVESLNVSVATGVLLAEWWRQNK
HHHCCHHHHHHHCCCCCCCCHHHCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHCCC
A
C
>Mature Secondary Structure 
SDELKNKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRVDGRSRPARDERNSRGD
CCCCCCCCCCEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCC
DRKRDDRKRDERKRDDFHRDDASPWRTVSRAPGDETPVKEDHGGISGKSFIDPEVLRRQR
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEAELAK
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEECCHHHHHH
ASGTEHHGGVCFLIKKRHGTSVAQWVAKAGEEDCVLALEDVGNPHNLGAIMRSCAHFGVK
HCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEHHHCCCCHHHHHHHHHHHHCCCC
GVVVQDAGVLESGAAIRTAEGGAEHVEPITGDSFIDTLDQFRKAGYAIVSTSSHNGTPLF
EEEEECCCCCCCCCEEEECCCCHHHCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCEEH
KAELPKKMVLALGQERDGLSDAAISSADLSVAIDGTGNVESLNVSVATGVLLAEWWRQNK
HHHCCHHHHHHHCCCCCCCCHHHCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]