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The map label for this gene is lepA [H]

Identifier: 238896020

GI number: 238896020

Start: 3981247

End: 3983046

Strand: Reverse

Name: lepA [H]

Synonym: KP1_4149

Alternate gene names: 238896020

Gene position: 3983046-3981247 (Counterclockwise)

Preceding gene: 238896021

Following gene: 238896019

Centisome position: 75.89

GC content: 58.33

Gene sequence:

>1800_bases
ATGAAGAATATACGTAACTTTTCGATCATCGCTCACATCGACCACGGTAAGTCGACGCTGTCTGACCGTATCATCCAGAT
TTGCGGTGGCCTTTCTGATCGTGAAATGGAAGCCCAGGTTCTGGACTCCATGGATCTTGAGCGCGAACGCGGGATCACCA
TCAAGGCGCAGAGCGTCACCCTGGACTATAAGGCCAGCGACGGTGAGACCTACCAGCTGAACTTTATCGACACCCCGGGC
CACGTGGACTTCTCTTATGAAGTTTCCCGTTCTCTTGCCGCGTGCGAAGGCGCGCTGCTGGTGGTAGACGCCGGGCAGGG
GGTAGAAGCCCAGACCCTGGCCAACTGCTACACCGCCATGGAGATGGATCTCGAAGTGGTTCCGGTACTCAACAAGATCG
ACCTGCCTGCGGCCGACCCTGAGCGCGTAGCGGACGAGATTGAAGATATCGTCGGGATCGACGCCCACGACGCGGTGCGC
TGCTCAGCGAAAACCGGCGTCGGCGTGACCGACGTGCTGGAGCGTCTGGTGCGCGATATCCCGCCGCCGGAAGGCGATCC
GGACGCGCCGCTGCAGGCGCTGATTATCGACTCCTGGTTCGATAACTACCTCGGCGTGGTGTCGCTGGTGCGGATTAAAA
ACGGCACCATGCGCAAGGGCGACAAGATCAAGGTCATGAGCACCGGCCAGGTCTATAACGCTGATCGTCTGGGCATCTTC
ACCCCGAAACAGGTTGACCGTACCGAGCTGAAATGCGGCGAGGTAGGCTGGCTGGTCTGCGCGATCAAAGACATCCTCGG
CGCGCCGGTGGGCGATACCCTGACCGCCGCCCGCAACCCGGCAGACAAAGCGCTGCCTGGCTTTAAGAAGGTGAAACCGC
AGGTGTATGCCGGCCTGTTCCCGGTGAGCTCCGATGATTATGAAGCTTTCCGCGACGCGCTGGGCAAACTGAGCCTTAAC
GATGCCTCTCTGTTCTACGAGCCGGAAAGCTCTACCGCGCTGGGCTTCGGCTTCCGCTGCGGCTTCCTCGGCCTGCTGCA
CATGGAGATCATTCAGGAGCGTCTGGAGCGTGAATACGATCTCGACCTGATCACCACCGCGCCGACGGTAGTCTACGAAG
TAGAGACCACCTCCAAAGAAGTTATCTACGTCGACAGCCCGTCCAAGCTGCCGCCGCTGAATAATATTCAGGAGCTGCGC
GAGCCGATCGCCGAGTGTCACATGCTGCTGCCGCAGGAGTTCCTCGGCAACGTGATTACCTTGTGTGTCGAGAAGCGTGG
GGTGCAGACCAACATGGTTTACCACGGTAAACAGGTGGCGCTGACCTATGAAATTCCGATGGCGGAAGTGGTTCTCGACT
TCTTCGACCGCCTGAAGTCCACCTCCCGCGGCTATGCGTCGCTGGACTACAACTTCAAACGCTTCCAGGCGTCGAACATG
GTACGCGTCGATGTGCTGATCAACGGTGAGCGCGTCGATGCGCTGGCGCTGATCACCCACAACGATAACGCGCCGTATCG
CGGCCGCGAGCTGGTGGAAAAGATGAAAGATCTGATTCCGCGTCAGCAGTTCGATATCGCGATTCAGGCGGCCATCGGTA
ACCACATTATCGCGCGCTCTACGGTGAAACAGCTGCGTAAAAACGTCCTGGCGAAATGCTACGGCGGCGACGTCAGCCGT
AAGAAAAAGCTGCTGCAGAAGCAGAAAGAAGGTAAGAAGCGTATGAAGCAGGTCGGTAACGTTGAGCTGCCGCAGGAAGC
GTTCCTCGCCATTCTGCACGTTGGTAAAGACGGCAAATAA

Upstream 100 bases:

>100_bases
TTTCGCGGATGTAGTGTAGAATGCGGCGTTTTCGGATTAAAAAACGTCAGGCACAGTACAGCGGCCTCATGATACTCGTA
AGGCATAATTAACTCTAAAT

Downstream 100 bases:

>100_bases
CCCTAAGGAGTTGGCATGGCGAACATGTTTGCCCTGATCCTGGTGATTGCAACCCTGGTGACGGGCGTTTTATGGTGCCT
GGACAAGTTCATTTTTGCAC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVTLDYKASDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVADEIEDIVGIDAHDAVR
CSAKTGVGVTDVLERLVRDIPPPEGDPDAPLQALIIDSWFDNYLGVVSLVRIKNGTMRKGDKIKVMSTGQVYNADRLGIF
TPKQVDRTELKCGEVGWLVCAIKDILGAPVGDTLTAARNPADKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLN
DASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSKEVIYVDSPSKLPPLNNIQELR
EPIAECHMLLPQEFLGNVITLCVEKRGVQTNMVYHGKQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASNM
VRVDVLINGERVDALALITHNDNAPYRGRELVEKMKDLIPRQQFDIAIQAAIGNHIIARSTVKQLRKNVLAKCYGGDVSR
KKKLLQKQKEGKKRMKQVGNVELPQEAFLAILHVGKDGK

Sequences:

>Translated_599_residues
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVTLDYKASDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVADEIEDIVGIDAHDAVR
CSAKTGVGVTDVLERLVRDIPPPEGDPDAPLQALIIDSWFDNYLGVVSLVRIKNGTMRKGDKIKVMSTGQVYNADRLGIF
TPKQVDRTELKCGEVGWLVCAIKDILGAPVGDTLTAARNPADKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLN
DASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSKEVIYVDSPSKLPPLNNIQELR
EPIAECHMLLPQEFLGNVITLCVEKRGVQTNMVYHGKQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASNM
VRVDVLINGERVDALALITHNDNAPYRGRELVEKMKDLIPRQQFDIAIQAAIGNHIIARSTVKQLRKNVLAKCYGGDVSR
KKKLLQKQKEGKKRMKQVGNVELPQEAFLAILHVGKDGK
>Mature_599_residues
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVTLDYKASDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVADEIEDIVGIDAHDAVR
CSAKTGVGVTDVLERLVRDIPPPEGDPDAPLQALIIDSWFDNYLGVVSLVRIKNGTMRKGDKIKVMSTGQVYNADRLGIF
TPKQVDRTELKCGEVGWLVCAIKDILGAPVGDTLTAARNPADKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLN
DASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSKEVIYVDSPSKLPPLNNIQELR
EPIAECHMLLPQEFLGNVITLCVEKRGVQTNMVYHGKQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASNM
VRVDVLINGERVDALALITHNDNAPYRGRELVEKMKDLIPRQQFDIAIQAAIGNHIIARSTVKQLRKNVLAKCYGGDVSR
KKKLLQKQKEGKKRMKQVGNVELPQEAFLAILHVGKDGK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=47.0881863560732, Blast_Score=592, Evalue=1e-169,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=45.8646616541353, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI25306283, Length=149, Percent_Identity=43.6241610738255, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI19923640, Length=152, Percent_Identity=42.7631578947368, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI25306287, Length=152, Percent_Identity=42.7631578947368, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=42.3611111111111, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI18390331, Length=184, Percent_Identity=33.1521739130435, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=43.6363636363636, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=43.6363636363636, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=43.6363636363636, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI53729339, Length=239, Percent_Identity=31.3807531380753, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI53729337, Length=239, Percent_Identity=31.3807531380753, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI217272894, Length=167, Percent_Identity=32.3353293413174, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI217272892, Length=167, Percent_Identity=32.3353293413174, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788922, Length=599, Percent_Identity=95.1585976627713, Blast_Score=1134, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=505, Percent_Identity=28.7128712871287, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=35.4838709677419, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=32.6923076923077, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789559, Length=229, Percent_Identity=28.82096069869, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1790412, Length=327, Percent_Identity=24.7706422018349, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1789737, Length=327, Percent_Identity=24.7706422018349, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=42.3202614379085, Blast_Score=493, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17556745, Length=468, Percent_Identity=25.2136752136752, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17533571, Length=161, Percent_Identity=35.4037267080745, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=157, Percent_Identity=36.9426751592357, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=35.8208955223881, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=35.8208955223881, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17552882, Length=169, Percent_Identity=32.5443786982249, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32566303, Length=246, Percent_Identity=27.2357723577236, Blast_Score=69, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71994658, Length=225, Percent_Identity=29.3333333333333, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25141371, Length=240, Percent_Identity=27.0833333333333, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=601, Percent_Identity=46.4226289517471, Blast_Score=535, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=43.0555555555556, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=43.0555555555556, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=182, Percent_Identity=36.2637362637363, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=38.2608695652174, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=39.5833333333333, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=241, Percent_Identity=26.1410788381743, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325337, Length=272, Percent_Identity=24.6323529411765, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319594, Length=272, Percent_Identity=24.6323529411765, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=42.7620632279534, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=36.0248447204969, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=43.4782608695652, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24585709, Length=149, Percent_Identity=36.241610738255, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585711, Length=149, Percent_Identity=36.241610738255, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24585713, Length=149, Percent_Identity=36.241610738255, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.9261744966443, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=35.3383458646617, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28572034, Length=244, Percent_Identity=29.0983606557377, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281363316, Length=281, Percent_Identity=25.9786476868327, Blast_Score=71, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17864358, Length=281, Percent_Identity=25.9786476868327, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66436; Mature: 66436

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAM
EEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPVLNKIDLPAADPERVADEIEDIVGIDAHDAVRCSAKTGVGVTDVLERLVRDI
HCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PPPEGDPDAPLQALIIDSWFDNYLGVVSLVRIKNGTMRKGDKIKVMSTGQVYNADRLGIF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEECCCCCCCC
TPKQVDRTELKCGEVGWLVCAIKDILGAPVGDTLTAARNPADKALPGFKKVKPQVYAGLF
CCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCHHHCCCCHHHCCCHHHEEEE
PVSSDDYEAFRDALGKLSLNDASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVETTSKEVIYVDSPSKLPPLNNIQELREPIAECHMLLPQEFLGNVIT
CEEEECCCEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
LCVEKRGVQTNMVYHGKQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASNM
HHHHHCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCE
VRVDVLINGERVDALALITHNDNAPYRGRELVEKMKDLIPRQQFDIAIQAAIGNHIIARS
EEEEEEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHCEEEEEHHCCHHHHHH
TVKQLRKNVLAKCYGGDVSRKKKLLQKQKEGKKRMKQVGNVELPQEAFLAILHVGKDGK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAM
EEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPVLNKIDLPAADPERVADEIEDIVGIDAHDAVRCSAKTGVGVTDVLERLVRDI
HCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PPPEGDPDAPLQALIIDSWFDNYLGVVSLVRIKNGTMRKGDKIKVMSTGQVYNADRLGIF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEECCCCCCCC
TPKQVDRTELKCGEVGWLVCAIKDILGAPVGDTLTAARNPADKALPGFKKVKPQVYAGLF
CCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCHHHCCCCHHHCCCHHHEEEE
PVSSDDYEAFRDALGKLSLNDASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVETTSKEVIYVDSPSKLPPLNNIQELREPIAECHMLLPQEFLGNVIT
CEEEECCCEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
LCVEKRGVQTNMVYHGKQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASNM
HHHHHCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCE
VRVDVLINGERVDALALITHNDNAPYRGRELVEKMKDLIPRQQFDIAIQAAIGNHIIARS
EEEEEEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHCEEEEEHHCCHHHHHH
TVKQLRKNVLAKCYGGDVSRKKKLLQKQKEGKKRMKQVGNVELPQEAFLAILHVGKDGK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA