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The map label for this gene is nuoI [H]

Identifier: 238895798

GI number: 238895798

Start: 3744392

End: 3744934

Strand: Reverse

Name: nuoI [H]

Synonym: KP1_3914

Alternate gene names: 238895798

Gene position: 3744934-3744392 (Counterclockwise)

Preceding gene: 238895799

Following gene: 238895797

Centisome position: 71.35

GC content: 56.91

Gene sequence:

>543_bases
ATGACCTTAAAAGAATTATTGGTGGGGTTTGGCACCCAGGTCCGTAGTATCTGGATGATTGGCCTGCATGCCTTCGCCAA
ACGTGAAACCCGGATGTATCCGGAAGAGCCGGTCTACCTGCCGCCGCGCTATCGCGGCCGCATCGTGCTGACCCGCGATC
CGGACGGTGCAGAGCGCTGCGTCGCCTGTAACCTGTGTGCGGTAGCCTGCCCGGTGGGCTGTATCTCGCTGCAGAAAGCA
GAGACGGTAGACGGCCGCTGGTATCCGGAGTTCTTCCGCATCAACTTCTCACGTTGCATCTTCTGCGGCCTGTGCGAAGA
AGCGTGCCCGACCACGGCGATCCAGTTGACCCCGGACTTCGAGCTGGGTGAATACAAGCGTCAGGATCTGGTGTACGAAA
AAGAGGATCTGCTGATTTCCGGTCCGGGCAAATACCCGGAATATAACTTCTACCGGATGGCGGGTATGGCAATCGACGGC
AAAGATAAGGGCGAAGCGGAAAATGAAGCTAAGCCAATCGACGTCAAGAGCCTGTTACCGTAA

Upstream 100 bases:

>100_bases
TGTCCTTTGGCTGGAAGGTATGCCTGCCGTTAACGCTGGTCAACTTGTTGGTAACGGCGGCTGTCATTCTCTGGCAGGCG
CAATAAGGGGTGAATTGAAC

Downstream 100 bases:

>100_bases
GGAGAGGGGCAATGGAATTCGCTTTTTATATCTGTGGCCTGATAGCCATCCTCGCAACCTTGCGGGTCGTCACCCACACC
AATCCGGTGCATGCGCTGCT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I; NUO9 [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRYRGRIVLTRDPDGAERCVACNLCAVACPVGCISLQKA
ETVDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFELGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
KDKGEAENEAKPIDVKSLLP

Sequences:

>Translated_180_residues
MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRYRGRIVLTRDPDGAERCVACNLCAVACPVGCISLQKA
ETVDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFELGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
KDKGEAENEAKPIDVKSLLP
>Mature_179_residues
TLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRYRGRIVLTRDPDGAERCVACNLCAVACPVGCISLQKAE
TVDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFELGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDGK
DKGEAENEAKPIDVKSLLP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=127, Percent_Identity=39.3700787401575, Blast_Score=100, Evalue=8e-22,
Organism=Escherichia coli, GI1788617, Length=180, Percent_Identity=98.3333333333333, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1789075, Length=146, Percent_Identity=28.7671232876712, Blast_Score=70, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17555194, Length=145, Percent_Identity=40, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17864306, Length=132, Percent_Identity=40.1515151515151, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 20433; Mature: 20302

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRYRGRIVLTRDPDGAERC
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEECCCCCCHH
VACNLCAVACPVGCISLQKAETVDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDF
HHHHHHHHHCCCCCEECCCCCCCCCCCCCHHEEECHHHEEEEEHHHHCCCCCEEEECCCC
ELGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDGKDKGEAENEAKPIDVKSLLP
CCCCHHHHCCEECCCCEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
TLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRYRGRIVLTRDPDGAERC
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEECCCCCCHH
VACNLCAVACPVGCISLQKAETVDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDF
HHHHHHHHHCCCCCEECCCCCCCCCCCCCHHEEECHHHEEEEEHHHHCCCCCEEEECCCC
ELGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDGKDKGEAENEAKPIDVKSLLP
CCCCHHHHCCEECCCCEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]