Click here to switch to the map view.

The map label for this gene is menE [H]

Identifier: 238895785

GI number: 238895785

Start: 3729889

End: 3731265

Strand: Reverse

Name: menE [H]

Synonym: KP1_3898

Alternate gene names: 238895785

Gene position: 3731265-3729889 (Counterclockwise)

Preceding gene: 238895786

Following gene: 238895783

Centisome position: 71.09

GC content: 62.75

Gene sequence:

>1377_bases
GTGACCTTTAACGACTGGCCCTGGCGGCACTGGCGTCAGCGGCGCGGAGAGGCCCTCGCCTTACGTCTGAATAACCAGCC
GCTGACCTGGCGGGAGCTATGCGCGCGGGTTGACGCGCTGGCGTCTGGTTTTGCCGCGCAGGGCGTAATGGAAGGGCAGG
GAGTGGCGCTCCGGGCCTATAACCAGCCGGAAACTCTGCTGGCATGGCTGGCGCTGCTACAGTGCGGGGCGCGGGTGCTG
CCGCTGAATCCGCAGCTGCCAGCGGTGTTGCTGCAGGCGTTGTTACCCGCGTTAACCATGCAGCATCAGCTGGTGCTTAA
CGGTGATACGCTGCCGGGGAATTTACCGGCGTTAACTCTGCAGGCGGCAGAAGGCGCTTGTGCCGTTTGTTGGCATGGCG
ATCGGTTGGTGTCGATGACGTTGACCTCCGGTTCAACCGGCCTGCCGAAAGCGGCGGTACATAGCGCCAACGCCCATCTG
GCCAGCGCGGCTGGCGTGCTGGCGCTGATGCCGTTTGCCGCAGGAGATGACTGGCTGCTGTCGCTGCCGTTGTTCCATGT
CTCCGGTCAGGGGATCGTCTGGCGCTGGCTGCTGGCCGGCGCGCGACTCACGGTACGTGACAAACAGCCGCTGGCGCAGA
TGCTGCACGGCTGTACCCATGCTTCGCTGGTGCCGACTCAGCTATGGCGCCTGCTGAATGATGATGCTGCGGTTTCCCTC
AAGGCGGTGCTGCTCGGCGGCGCGTCGATCCCGGTCGAATTGACTGAACGGGCGCGAAAGCAGGGCATACGCAGCTTCTG
CGGCTATGGCCTCACCGAATTCGCCTCCACCGTGTGTGCCAAAGAGGCCGACGGCGCTGCGGATGTTGGCGAAGCGCTGC
CGGGGCGAGAGGTGAAAATTGTCGCCGGCGAGATCTGGCTGCGGGCGTCGAGTATGGCCGCAGGCTACTGGCGGGATGGT
CAATTGCTGTCATTAACCAACAATGAAGGTTGGTTTGCGACGCGCGATCACGGAGCATTGCATAACGGTCGGCTGACGGT
TGTTGGGCGACTGGATAACCTCTTTTTTAGCGGTGGAGAAGGTATTCAACCGGAAGAGGTCGAGCGTGTGATACTCGCCC
ATCCACAGGTACAGCAGGTGTTTATCGTGCCGCTGGATGATGCGGAATATGGCCAGCGCCCGGTAGCGGTGGTGGAATGC
GATGACGGCTGCGAGTTGAGCGCGCTGGCGGCATGGAGCGCTGAGCGGTTAGCGCGTTTTCAGCGGCCGGTGCGTTGGCT
GCGGTTGCCGGAAACGTTAAAAAACGGCGGGATTAAGATTTCGCGCCGGGCGCTGTGCGAGTGGGTCAGGCAGCAGACGC
ATGCGACCGTATCGTAG

Upstream 100 bases:

>100_bases
CTGGGCTGGATACGCTGGCGCTGATGGGCGCCCAGCTGGTACGGCCGTGGCCCGAAAGTGCGCTGCCGGTGCTCAACATT
GATGCGCTGGAGCCGCTGCT

Downstream 100 bases:

>100_bases
CCACCCGCATTATCCCGCGGCATAACGCCGGGTGGGTTGAACGCGGTATGCTGGACTAAAGTAGCCCTTTCAGGCTGAGA
GTAACGCGGGTGGCGGCATT

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTFNDWPWRHWRQRRGEALALRLNNQPLTWRELCARVDALASGFAAQGVMEGQGVALRAYNQPETLLAWLALLQCGARVL
PLNPQLPAVLLQALLPALTMQHQLVLNGDTLPGNLPALTLQAAEGACAVCWHGDRLVSMTLTSGSTGLPKAAVHSANAHL
ASAAGVLALMPFAAGDDWLLSLPLFHVSGQGIVWRWLLAGARLTVRDKQPLAQMLHGCTHASLVPTQLWRLLNDDAAVSL
KAVLLGGASIPVELTERARKQGIRSFCGYGLTEFASTVCAKEADGAADVGEALPGREVKIVAGEIWLRASSMAAGYWRDG
QLLSLTNNEGWFATRDHGALHNGRLTVVGRLDNLFFSGGEGIQPEEVERVILAHPQVQQVFIVPLDDAEYGQRPVAVVEC
DDGCELSALAAWSAERLARFQRPVRWLRLPETLKNGGIKISRRALCEWVRQQTHATVS

Sequences:

>Translated_458_residues
MTFNDWPWRHWRQRRGEALALRLNNQPLTWRELCARVDALASGFAAQGVMEGQGVALRAYNQPETLLAWLALLQCGARVL
PLNPQLPAVLLQALLPALTMQHQLVLNGDTLPGNLPALTLQAAEGACAVCWHGDRLVSMTLTSGSTGLPKAAVHSANAHL
ASAAGVLALMPFAAGDDWLLSLPLFHVSGQGIVWRWLLAGARLTVRDKQPLAQMLHGCTHASLVPTQLWRLLNDDAAVSL
KAVLLGGASIPVELTERARKQGIRSFCGYGLTEFASTVCAKEADGAADVGEALPGREVKIVAGEIWLRASSMAAGYWRDG
QLLSLTNNEGWFATRDHGALHNGRLTVVGRLDNLFFSGGEGIQPEEVERVILAHPQVQQVFIVPLDDAEYGQRPVAVVEC
DDGCELSALAAWSAERLARFQRPVRWLRLPETLKNGGIKISRRALCEWVRQQTHATVS
>Mature_457_residues
TFNDWPWRHWRQRRGEALALRLNNQPLTWRELCARVDALASGFAAQGVMEGQGVALRAYNQPETLLAWLALLQCGARVLP
LNPQLPAVLLQALLPALTMQHQLVLNGDTLPGNLPALTLQAAEGACAVCWHGDRLVSMTLTSGSTGLPKAAVHSANAHLA
SAAGVLALMPFAAGDDWLLSLPLFHVSGQGIVWRWLLAGARLTVRDKQPLAQMLHGCTHASLVPTQLWRLLNDDAAVSLK
AVLLGGASIPVELTERARKQGIRSFCGYGLTEFASTVCAKEADGAADVGEALPGREVKIVAGEIWLRASSMAAGYWRDGQ
LLSLTNNEGWFATRDHGALHNGRLTVVGRLDNLFFSGGEGIQPEEVERVILAHPQVQQVFIVPLDDAEYGQRPVAVVECD
DGCELSALAAWSAERLARFQRPVRWLRLPETLKNGGIKISRRALCEWVRQQTHATVS

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=517, Percent_Identity=22.8239845261122, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI187761343, Length=517, Percent_Identity=22.8239845261122, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788595, Length=452, Percent_Identity=73.8938053097345, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=471, Percent_Identity=25.9023354564756, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788107, Length=350, Percent_Identity=25.7142857142857, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI32563687, Length=474, Percent_Identity=24.8945147679325, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17558820, Length=474, Percent_Identity=25.7383966244726, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=361, Percent_Identity=27.1468144044321, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17557194, Length=494, Percent_Identity=23.4817813765182, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17538037, Length=465, Percent_Identity=23.010752688172, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71994690, Length=330, Percent_Identity=26.0606060606061, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994703, Length=330, Percent_Identity=26.0606060606061, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994694, Length=330, Percent_Identity=26.0606060606061, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=294, Percent_Identity=26.1904761904762, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71996755, Length=295, Percent_Identity=24.7457627118644, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=243, Percent_Identity=25.1028806584362, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24581924, Length=512, Percent_Identity=23.4375, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI18859661, Length=352, Percent_Identity=27.8409090909091, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24653035, Length=323, Percent_Identity=25.077399380805, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 49790; Mature: 49659

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFNDWPWRHWRQRRGEALALRLNNQPLTWRELCARVDALASGFAAQGVMEGQGVALRAY
CCCCCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCEEEEEC
NQPETLLAWLALLQCGARVLPLNPQLPAVLLQALLPALTMQHQLVLNGDTLPGNLPALTL
CCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEE
QAAEGACAVCWHGDRLVSMTLTSGSTGLPKAAVHSANAHLASAAGVLALMPFAAGDDWLL
EECCCCEEEEEECCEEEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHEECCCCCCCEEE
SLPLFHVSGQGIVWRWLLAGARLTVRDKQPLAQMLHGCTHASLVPTQLWRLLNDDAAVSL
EEEEEEECCCCCEEEHHHCCCEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHEE
KAVLLGGASIPVELTERARKQGIRSFCGYGLTEFASTVCAKEADGAADVGEALPGREVKI
EEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHCCCCCEEEE
VAGEIWLRASSMAAGYWRDGQLLSLTNNEGWFATRDHGALHNGRLTVVGRLDNLFFSGGE
EEEEEEEEECCCCCCEECCCEEEEEECCCCEEEECCCCCEECCEEEEEEEECHHEECCCC
GIQPEEVERVILAHPQVQQVFIVPLDDAEYGQRPVAVVECDDGCELSALAAWSAERLARF
CCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
QRPVRWLRLPETLKNGGIKISRRALCEWVRQQTHATVS
HHHHHHHCCCHHHHCCCEEEHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TFNDWPWRHWRQRRGEALALRLNNQPLTWRELCARVDALASGFAAQGVMEGQGVALRAY
CCCCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCEEEEEC
NQPETLLAWLALLQCGARVLPLNPQLPAVLLQALLPALTMQHQLVLNGDTLPGNLPALTL
CCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEE
QAAEGACAVCWHGDRLVSMTLTSGSTGLPKAAVHSANAHLASAAGVLALMPFAAGDDWLL
EECCCCEEEEEECCEEEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHEECCCCCCCEEE
SLPLFHVSGQGIVWRWLLAGARLTVRDKQPLAQMLHGCTHASLVPTQLWRLLNDDAAVSL
EEEEEEECCCCCEEEHHHCCCEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHEE
KAVLLGGASIPVELTERARKQGIRSFCGYGLTEFASTVCAKEADGAADVGEALPGREVKI
EEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHCCCCCEEEE
VAGEIWLRASSMAAGYWRDGQLLSLTNNEGWFATRDHGALHNGRLTVVGRLDNLFFSGGE
EEEEEEEEECCCCCCEECCCEEEEEECCCCEEEECCCCCEECCEEEEEEEECHHEECCCC
GIQPEEVERVILAHPQVQQVFIVPLDDAEYGQRPVAVVECDDGCELSALAAWSAERLARF
CCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
QRPVRWLRLPETLKNGGIKISRRALCEWVRQQTHATVS
HHHHHHHCCCHHHHCCCEEEHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]